Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates
The global landscape of Candida infections has seen a significant shift. Previously, Candida albicans was the predominant species. However, there has been an emergence of non‐albicans Candida species, which are often less susceptible to antifungal treatment. Candida kefyr, in particular, has been in...
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| Veröffentlicht in: | Environmental microbiology reports 2024-06, Vol.16 (3), p.e13282-n/a |
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| creator | Nouraei, Hasti Zare, Samira Nemati, Maryam Amirzadeh, Neda Motamedi, Marjan Shabanzadeh, Shafigheh Zomorodian, Kamiar Pakshir, Keyvan |
| description | The global landscape of Candida infections has seen a significant shift. Previously, Candida albicans was the predominant species. However, there has been an emergence of non‐albicans Candida species, which are often less susceptible to antifungal treatment. Candida kefyr, in particular, has been increasingly associated with infections. This study aimed to investigate the profiles of enzymatic activity and biofilm formation in both clinical and non‐clinical isolates of C. kefyr. A total of 66 C. kefyr isolates were analysed. The activities of proteinase and phospholipase were assessed using bovine serum albumin and egg yolk agar, respectively. Haemolysin, caseinolytic and esterase activities were evaluated using specific methods. Biofilm formation was investigated using crystal violet staining. The findings indicated that biofilm and proteinase activity were detected in 81.8% and 93.9% of all the isolates, respectively. Haemolysin activity was observed with the highest occurrence (95.5%) among normal microbiota isolates. Esterase activity was predominantly identified in dairy samples and was absent in hospital samples. Caseinase production was found with the highest occurrence (18.2%) in normal microbiota and hospital samples. Phospholipase activity was limited, found in only 3% of all the isolates. These findings reveal variations in enzyme activity between clinical and non‐clinical C. kefyr isolates. This sheds light on their pathogenic potential and has implications for therapeutic strategies.
This study investigates the enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates. Findings reveal significant biofilm, proteinase and haemolysin activities across isolates. Esterase activity was mainly found in dairy samples, while caseinase production was prevalent in normal microbiota and hospital samples. Phospholipase activity was minimal. The study highlights the pathogenic potential of C. kefyr and its implications for therapeutic strategies. |
| doi_str_mv | 10.1111/1758-2229.13282 |
| format | Article |
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This study investigates the enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates. Findings reveal significant biofilm, proteinase and haemolysin activities across isolates. Esterase activity was mainly found in dairy samples, while caseinase production was prevalent in normal microbiota and hospital samples. Phospholipase activity was minimal. The study highlights the pathogenic potential of C. kefyr and its implications for therapeutic strategies.</description><identifier>ISSN: 1758-2229</identifier><identifier>EISSN: 1758-2229</identifier><identifier>DOI: 10.1111/1758-2229.13282</identifier><identifier>PMID: 38923398</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Biofilms - growth & development ; Brief Report ; Brief Reports ; Candida - classification ; Candida - enzymology ; Candida - isolation & purification ; Candida - physiology ; Candidiasis - microbiology ; Environmental Microbiology ; Esterases - metabolism ; Hemolysin Proteins - metabolism ; Humans ; Peptide Hydrolases - metabolism ; Phospholipases - metabolism</subject><ispartof>Environmental microbiology reports, 2024-06, Vol.16 (3), p.e13282-n/a</ispartof><rights>2024 The Author(s). published by John Wiley & Sons Ltd.</rights><rights>2024 The Author(s). Environmental Microbiology Reports published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3252-16e9a4678d21f87ae5fa41891298d752bce9862463896df97e402869c6f3500b3</cites><orcidid>0000-0001-7809-4690</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194042/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194042/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,1418,11567,27929,27930,45579,45580,46057,46481,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38923398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nouraei, Hasti</creatorcontrib><creatorcontrib>Zare, Samira</creatorcontrib><creatorcontrib>Nemati, Maryam</creatorcontrib><creatorcontrib>Amirzadeh, Neda</creatorcontrib><creatorcontrib>Motamedi, Marjan</creatorcontrib><creatorcontrib>Shabanzadeh, Shafigheh</creatorcontrib><creatorcontrib>Zomorodian, Kamiar</creatorcontrib><creatorcontrib>Pakshir, Keyvan</creatorcontrib><title>Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates</title><title>Environmental microbiology reports</title><addtitle>Environ Microbiol Rep</addtitle><description>The global landscape of Candida infections has seen a significant shift. Previously, Candida albicans was the predominant species. However, there has been an emergence of non‐albicans Candida species, which are often less susceptible to antifungal treatment. Candida kefyr, in particular, has been increasingly associated with infections. This study aimed to investigate the profiles of enzymatic activity and biofilm formation in both clinical and non‐clinical isolates of C. kefyr. A total of 66 C. kefyr isolates were analysed. The activities of proteinase and phospholipase were assessed using bovine serum albumin and egg yolk agar, respectively. Haemolysin, caseinolytic and esterase activities were evaluated using specific methods. Biofilm formation was investigated using crystal violet staining. The findings indicated that biofilm and proteinase activity were detected in 81.8% and 93.9% of all the isolates, respectively. Haemolysin activity was observed with the highest occurrence (95.5%) among normal microbiota isolates. Esterase activity was predominantly identified in dairy samples and was absent in hospital samples. Caseinase production was found with the highest occurrence (18.2%) in normal microbiota and hospital samples. Phospholipase activity was limited, found in only 3% of all the isolates. These findings reveal variations in enzyme activity between clinical and non‐clinical C. kefyr isolates. This sheds light on their pathogenic potential and has implications for therapeutic strategies.
This study investigates the enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates. Findings reveal significant biofilm, proteinase and haemolysin activities across isolates. Esterase activity was mainly found in dairy samples, while caseinase production was prevalent in normal microbiota and hospital samples. Phospholipase activity was minimal. The study highlights the pathogenic potential of C. kefyr and its implications for therapeutic strategies.</description><subject>Biofilms - growth & development</subject><subject>Brief Report</subject><subject>Brief Reports</subject><subject>Candida - classification</subject><subject>Candida - enzymology</subject><subject>Candida - isolation & purification</subject><subject>Candida - physiology</subject><subject>Candidiasis - microbiology</subject><subject>Environmental Microbiology</subject><subject>Esterases - metabolism</subject><subject>Hemolysin Proteins - metabolism</subject><subject>Humans</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Phospholipases - metabolism</subject><issn>1758-2229</issn><issn>1758-2229</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNqFkUtvGyEUhVHVqHm06-4qlt04AYZhYFVVlvOQEmWTrBFmLi0tAy7Yjia_PkycWOkqbIBzDx8XDkJfKTmldZzRrpUzxpg6pQ2T7AM62isf36wP0XEpfwgRXBH2CR02UrGmUfIIbeZpWJls1n4L2EQTxuILTg5DfByHKlu8ysn5AKWWe7z002bALuWpmiL2Edvgo7cmPDsgbn1OcYC4rsq8Sr43-C-4MWNfUjBrKJ_RgTOhwJeX-QTdny_u5pez69uLq_nP65ltWMtmVIAyXHSyZ9TJzkDrDKdSUaZk37VsaUFJwbio7xG9Ux1wwqRQVrimJWTZnKAfO-5qsxygt7WnbIJeZT-YPOpkvP6_Ev1v_Sttdf1bxQlnlfD9hZDTvw2UtR58sRCCiZA2RTekY52SvJusZzurzamUDG5_DyUTkOopDz3loZ_Tqie-vW1v73-NpxrEzvBQAxjf4-nFzRXfkZ8AMtShig</recordid><startdate>202406</startdate><enddate>202406</enddate><creator>Nouraei, Hasti</creator><creator>Zare, Samira</creator><creator>Nemati, Maryam</creator><creator>Amirzadeh, Neda</creator><creator>Motamedi, Marjan</creator><creator>Shabanzadeh, Shafigheh</creator><creator>Zomorodian, Kamiar</creator><creator>Pakshir, Keyvan</creator><general>John Wiley & Sons, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7809-4690</orcidid></search><sort><creationdate>202406</creationdate><title>Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates</title><author>Nouraei, Hasti ; Zare, Samira ; Nemati, Maryam ; Amirzadeh, Neda ; Motamedi, Marjan ; Shabanzadeh, Shafigheh ; Zomorodian, Kamiar ; Pakshir, Keyvan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3252-16e9a4678d21f87ae5fa41891298d752bce9862463896df97e402869c6f3500b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biofilms - growth & development</topic><topic>Brief Report</topic><topic>Brief Reports</topic><topic>Candida - classification</topic><topic>Candida - enzymology</topic><topic>Candida - isolation & purification</topic><topic>Candida - physiology</topic><topic>Candidiasis - microbiology</topic><topic>Environmental Microbiology</topic><topic>Esterases - metabolism</topic><topic>Hemolysin Proteins - metabolism</topic><topic>Humans</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Phospholipases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nouraei, Hasti</creatorcontrib><creatorcontrib>Zare, Samira</creatorcontrib><creatorcontrib>Nemati, Maryam</creatorcontrib><creatorcontrib>Amirzadeh, Neda</creatorcontrib><creatorcontrib>Motamedi, Marjan</creatorcontrib><creatorcontrib>Shabanzadeh, Shafigheh</creatorcontrib><creatorcontrib>Zomorodian, Kamiar</creatorcontrib><creatorcontrib>Pakshir, Keyvan</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Environmental microbiology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nouraei, Hasti</au><au>Zare, Samira</au><au>Nemati, Maryam</au><au>Amirzadeh, Neda</au><au>Motamedi, Marjan</au><au>Shabanzadeh, Shafigheh</au><au>Zomorodian, Kamiar</au><au>Pakshir, Keyvan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates</atitle><jtitle>Environmental microbiology reports</jtitle><addtitle>Environ Microbiol Rep</addtitle><date>2024-06</date><risdate>2024</risdate><volume>16</volume><issue>3</issue><spage>e13282</spage><epage>n/a</epage><pages>e13282-n/a</pages><issn>1758-2229</issn><eissn>1758-2229</eissn><abstract>The global landscape of Candida infections has seen a significant shift. Previously, Candida albicans was the predominant species. However, there has been an emergence of non‐albicans Candida species, which are often less susceptible to antifungal treatment. Candida kefyr, in particular, has been increasingly associated with infections. This study aimed to investigate the profiles of enzymatic activity and biofilm formation in both clinical and non‐clinical isolates of C. kefyr. A total of 66 C. kefyr isolates were analysed. The activities of proteinase and phospholipase were assessed using bovine serum albumin and egg yolk agar, respectively. Haemolysin, caseinolytic and esterase activities were evaluated using specific methods. Biofilm formation was investigated using crystal violet staining. The findings indicated that biofilm and proteinase activity were detected in 81.8% and 93.9% of all the isolates, respectively. Haemolysin activity was observed with the highest occurrence (95.5%) among normal microbiota isolates. Esterase activity was predominantly identified in dairy samples and was absent in hospital samples. Caseinase production was found with the highest occurrence (18.2%) in normal microbiota and hospital samples. Phospholipase activity was limited, found in only 3% of all the isolates. These findings reveal variations in enzyme activity between clinical and non‐clinical C. kefyr isolates. This sheds light on their pathogenic potential and has implications for therapeutic strategies.
This study investigates the enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates. Findings reveal significant biofilm, proteinase and haemolysin activities across isolates. Esterase activity was mainly found in dairy samples, while caseinase production was prevalent in normal microbiota and hospital samples. Phospholipase activity was minimal. The study highlights the pathogenic potential of C. kefyr and its implications for therapeutic strategies.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>38923398</pmid><doi>10.1111/1758-2229.13282</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-7809-4690</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley-Blackwell Open Access Titles; Wiley Online Library All Journals; PubMed Central |
| subjects | Biofilms - growth & development Brief Report Brief Reports Candida - classification Candida - enzymology Candida - isolation & purification Candida - physiology Candidiasis - microbiology Environmental Microbiology Esterases - metabolism Hemolysin Proteins - metabolism Humans Peptide Hydrolases - metabolism Phospholipases - metabolism |
| title | Comparative analysis of enzymatic profiles and biofilm formation in clinical and environmental Candida kefyr isolates |
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