Brassica rapa CURLY LEAF is a major H3K27 methyltransferase regulating flowering time
Main conclusion In Brassica rapa , the epigenetic modifier BraA.CLF orchestrates flowering by modulating H3K27me3 levels at the floral integrator genes FT , SOC1 , and SEP3 , thereby influencing their expression. CURLY LEAF (CLF) is the catalytic subunit of the plant Polycomb Repressive Complex 2 th...
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| creator | Poza-Viejo, Laura Payá-Milans, Miriam Wilkinson, Mark D. Piñeiro, Manuel Jarillo, José A. Crevillén, Pedro |
| description | Main conclusion
In
Brassica rapa
, the epigenetic modifier BraA.CLF orchestrates flowering by modulating H3K27me3 levels at the floral integrator genes
FT
,
SOC1
, and
SEP3
, thereby influencing their expression.
CURLY LEAF (CLF) is the catalytic subunit of the plant Polycomb Repressive Complex 2 that mediates the trimethylation of histone H3 lysine 27 (H3K27me3), an epigenetic modification that leads to gene silencing. While the function of CURLY LEAF (CLF) has been extensively studied in
Arabidopsis thaliana
, its role in Brassica crops is barely known. In this study, we focused on the
Brassica rapa
homolog of
CLF
and found that the loss-of-function mutant
braA.clf-1
exhibits an accelerated flowering together with pleiotropic phenotypic alterations compared to wild-type plants. In addition, we carried out transcriptomic and H3K27me3 genome-wide analyses to identify the genes regulated by BraA.CLF. Interestingly, we observed that several floral regulatory genes, including the
B. rapa
homologs of
FT
,
SOC1
and
SEP3
, show reduced H3K27me3 levels and increased transcript levels compared to wild-type plants, suggesting that they are direct targets of BraA.CLF and key players in regulating flowering time in this crop. In addition, the results obtained will enhance our understanding of the epigenetic mechanisms regulating key developmental traits and will aid to increase crop yield by engineering new Brassica varieties with different flowering time requirements. |
| doi_str_mv | 10.1007/s00425-024-04454-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11169032</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3068754513</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-dff59557fc29eb2aa77eff6c5fa2a596cd10ac589062f20f1b4ed86fabb4b0d63</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EokvhD3BAlrhwCYy_kxMqq5ZWrISE2AMna-LY26zysdgJqP8ehy2lcICTR5rnfT0zLyHPGbxmAOZNApBcFcBlAVIqWZgHZMWk4AUHWT4kK4BcQyXUCXmS0h4gN415TE5EWWoFrFyR7buIKbUOacQD0vX20-YL3ZyfXdA2UaQ97sdIL8UHbmjvp-ubboo4pOCzytPod3OHUzvsaOjG7z4u1dT2_il5FLBL_tnte0q2F-ef15fF5uP7q_XZpnBSVVPRhKAqpUxwvPI1RzTGh6CdCshRVdo1DNCpsgLNA4fAaumbUgesa1lDo8UpeXv0Pcx17xvnhzxeZw-x7THe2BFb-2dnaK_tbvxmGWO6ytfJDq9uHeL4dfZpsn2bnO86HPw4JyuYEloJBfr_KOjSKKmYyOjLv9D9OMchn2KhDHDNtckUP1IujilFH-4GZ2CXhO0xYZsTtj8Ttovoxf2V7yS_Is2AOALpsOTh4--__2H7A3aLsOM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3067026267</pqid></control><display><type>article</type><title>Brassica rapa CURLY LEAF is a major H3K27 methyltransferase regulating flowering time</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Poza-Viejo, Laura ; Payá-Milans, Miriam ; Wilkinson, Mark D. ; Piñeiro, Manuel ; Jarillo, José A. ; Crevillén, Pedro</creator><creatorcontrib>Poza-Viejo, Laura ; Payá-Milans, Miriam ; Wilkinson, Mark D. ; Piñeiro, Manuel ; Jarillo, José A. ; Crevillén, Pedro</creatorcontrib><description>Main conclusion
In
Brassica rapa
, the epigenetic modifier BraA.CLF orchestrates flowering by modulating H3K27me3 levels at the floral integrator genes
FT
,
SOC1
, and
SEP3
, thereby influencing their expression.
CURLY LEAF (CLF) is the catalytic subunit of the plant Polycomb Repressive Complex 2 that mediates the trimethylation of histone H3 lysine 27 (H3K27me3), an epigenetic modification that leads to gene silencing. While the function of CURLY LEAF (CLF) has been extensively studied in
Arabidopsis thaliana
, its role in Brassica crops is barely known. In this study, we focused on the
Brassica rapa
homolog of
CLF
and found that the loss-of-function mutant
braA.clf-1
exhibits an accelerated flowering together with pleiotropic phenotypic alterations compared to wild-type plants. In addition, we carried out transcriptomic and H3K27me3 genome-wide analyses to identify the genes regulated by BraA.CLF. Interestingly, we observed that several floral regulatory genes, including the
B. rapa
homologs of
FT
,
SOC1
and
SEP3
, show reduced H3K27me3 levels and increased transcript levels compared to wild-type plants, suggesting that they are direct targets of BraA.CLF and key players in regulating flowering time in this crop. In addition, the results obtained will enhance our understanding of the epigenetic mechanisms regulating key developmental traits and will aid to increase crop yield by engineering new Brassica varieties with different flowering time requirements.</description><identifier>ISSN: 0032-0935</identifier><identifier>ISSN: 1432-2048</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-024-04454-7</identifier><identifier>PMID: 38865018</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agriculture ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biomedical and Life Sciences ; Brassica ; Brassica rapa ; Brassica rapa - genetics ; Brassica rapa - growth & development ; Brassica rapa - physiology ; Crop yield ; Ecology ; Epigenesis, Genetic ; Epigenetics ; Flowering ; Flowers - genetics ; Flowers - growth & development ; Flowers - physiology ; Forestry ; Gene Expression Regulation, Plant ; Gene silencing ; Genes ; Histone H3 ; Histone-Lysine N-Methyltransferase - genetics ; Histone-Lysine N-Methyltransferase - metabolism ; Histones ; Histones - genetics ; Histones - metabolism ; Leaves ; Life Sciences ; loss-of-function mutation ; Lysine ; Methylation ; Methyltransferase ; methyltransferases ; mutants ; New varieties ; Original ; Original Article ; phenotype ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; Plants (botany) ; Polycomb group proteins ; protein subunits ; Transcriptomics</subject><ispartof>Planta, 2024-07, Vol.260 (1), p.27-27, Article 27</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c459t-dff59557fc29eb2aa77eff6c5fa2a596cd10ac589062f20f1b4ed86fabb4b0d63</cites><orcidid>0000-0003-1276-9792</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00425-024-04454-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00425-024-04454-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38865018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Poza-Viejo, Laura</creatorcontrib><creatorcontrib>Payá-Milans, Miriam</creatorcontrib><creatorcontrib>Wilkinson, Mark D.</creatorcontrib><creatorcontrib>Piñeiro, Manuel</creatorcontrib><creatorcontrib>Jarillo, José A.</creatorcontrib><creatorcontrib>Crevillén, Pedro</creatorcontrib><title>Brassica rapa CURLY LEAF is a major H3K27 methyltransferase regulating flowering time</title><title>Planta</title><addtitle>Planta</addtitle><addtitle>Planta</addtitle><description>Main conclusion
In
Brassica rapa
, the epigenetic modifier BraA.CLF orchestrates flowering by modulating H3K27me3 levels at the floral integrator genes
FT
,
SOC1
, and
SEP3
, thereby influencing their expression.
CURLY LEAF (CLF) is the catalytic subunit of the plant Polycomb Repressive Complex 2 that mediates the trimethylation of histone H3 lysine 27 (H3K27me3), an epigenetic modification that leads to gene silencing. While the function of CURLY LEAF (CLF) has been extensively studied in
Arabidopsis thaliana
, its role in Brassica crops is barely known. In this study, we focused on the
Brassica rapa
homolog of
CLF
and found that the loss-of-function mutant
braA.clf-1
exhibits an accelerated flowering together with pleiotropic phenotypic alterations compared to wild-type plants. In addition, we carried out transcriptomic and H3K27me3 genome-wide analyses to identify the genes regulated by BraA.CLF. Interestingly, we observed that several floral regulatory genes, including the
B. rapa
homologs of
FT
,
SOC1
and
SEP3
, show reduced H3K27me3 levels and increased transcript levels compared to wild-type plants, suggesting that they are direct targets of BraA.CLF and key players in regulating flowering time in this crop. In addition, the results obtained will enhance our understanding of the epigenetic mechanisms regulating key developmental traits and will aid to increase crop yield by engineering new Brassica varieties with different flowering time requirements.</description><subject>Agriculture</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biomedical and Life Sciences</subject><subject>Brassica</subject><subject>Brassica rapa</subject><subject>Brassica rapa - genetics</subject><subject>Brassica rapa - growth & development</subject><subject>Brassica rapa - physiology</subject><subject>Crop yield</subject><subject>Ecology</subject><subject>Epigenesis, Genetic</subject><subject>Epigenetics</subject><subject>Flowering</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Flowers - physiology</subject><subject>Forestry</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>Histone H3</subject><subject>Histone-Lysine N-Methyltransferase - genetics</subject><subject>Histone-Lysine N-Methyltransferase - metabolism</subject><subject>Histones</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>loss-of-function mutation</subject><subject>Lysine</subject><subject>Methylation</subject><subject>Methyltransferase</subject><subject>methyltransferases</subject><subject>mutants</subject><subject>New varieties</subject><subject>Original</subject><subject>Original Article</subject><subject>phenotype</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Sciences</subject><subject>Plants (botany)</subject><subject>Polycomb group proteins</subject><subject>protein subunits</subject><subject>Transcriptomics</subject><issn>0032-0935</issn><issn>1432-2048</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhD3BAlrhwCYy_kxMqq5ZWrISE2AMna-LY26zysdgJqP8ehy2lcICTR5rnfT0zLyHPGbxmAOZNApBcFcBlAVIqWZgHZMWk4AUHWT4kK4BcQyXUCXmS0h4gN415TE5EWWoFrFyR7buIKbUOacQD0vX20-YL3ZyfXdA2UaQ97sdIL8UHbmjvp-ubboo4pOCzytPod3OHUzvsaOjG7z4u1dT2_il5FLBL_tnte0q2F-ef15fF5uP7q_XZpnBSVVPRhKAqpUxwvPI1RzTGh6CdCshRVdo1DNCpsgLNA4fAaumbUgesa1lDo8UpeXv0Pcx17xvnhzxeZw-x7THe2BFb-2dnaK_tbvxmGWO6ytfJDq9uHeL4dfZpsn2bnO86HPw4JyuYEloJBfr_KOjSKKmYyOjLv9D9OMchn2KhDHDNtckUP1IujilFH-4GZ2CXhO0xYZsTtj8Ttovoxf2V7yS_Is2AOALpsOTh4--__2H7A3aLsOM</recordid><startdate>20240701</startdate><enddate>20240701</enddate><creator>Poza-Viejo, Laura</creator><creator>Payá-Milans, Miriam</creator><creator>Wilkinson, Mark D.</creator><creator>Piñeiro, Manuel</creator><creator>Jarillo, José A.</creator><creator>Crevillén, Pedro</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1276-9792</orcidid></search><sort><creationdate>20240701</creationdate><title>Brassica rapa CURLY LEAF is a major H3K27 methyltransferase regulating flowering time</title><author>Poza-Viejo, Laura ; Payá-Milans, Miriam ; Wilkinson, Mark D. ; Piñeiro, Manuel ; Jarillo, José A. ; Crevillén, Pedro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-dff59557fc29eb2aa77eff6c5fa2a596cd10ac589062f20f1b4ed86fabb4b0d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Agriculture</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biomedical and Life Sciences</topic><topic>Brassica</topic><topic>Brassica rapa</topic><topic>Brassica rapa - genetics</topic><topic>Brassica rapa - growth & development</topic><topic>Brassica rapa - physiology</topic><topic>Crop yield</topic><topic>Ecology</topic><topic>Epigenesis, Genetic</topic><topic>Epigenetics</topic><topic>Flowering</topic><topic>Flowers - genetics</topic><topic>Flowers - growth & development</topic><topic>Flowers - physiology</topic><topic>Forestry</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene silencing</topic><topic>Genes</topic><topic>Histone H3</topic><topic>Histone-Lysine N-Methyltransferase - genetics</topic><topic>Histone-Lysine N-Methyltransferase - metabolism</topic><topic>Histones</topic><topic>Histones - genetics</topic><topic>Histones - metabolism</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>loss-of-function mutation</topic><topic>Lysine</topic><topic>Methylation</topic><topic>Methyltransferase</topic><topic>methyltransferases</topic><topic>mutants</topic><topic>New varieties</topic><topic>Original</topic><topic>Original Article</topic><topic>phenotype</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Sciences</topic><topic>Plants (botany)</topic><topic>Polycomb group proteins</topic><topic>protein subunits</topic><topic>Transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poza-Viejo, Laura</creatorcontrib><creatorcontrib>Payá-Milans, Miriam</creatorcontrib><creatorcontrib>Wilkinson, Mark D.</creatorcontrib><creatorcontrib>Piñeiro, Manuel</creatorcontrib><creatorcontrib>Jarillo, José A.</creatorcontrib><creatorcontrib>Crevillén, Pedro</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poza-Viejo, Laura</au><au>Payá-Milans, Miriam</au><au>Wilkinson, Mark D.</au><au>Piñeiro, Manuel</au><au>Jarillo, José A.</au><au>Crevillén, Pedro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Brassica rapa CURLY LEAF is a major H3K27 methyltransferase regulating flowering time</atitle><jtitle>Planta</jtitle><stitle>Planta</stitle><addtitle>Planta</addtitle><date>2024-07-01</date><risdate>2024</risdate><volume>260</volume><issue>1</issue><spage>27</spage><epage>27</epage><pages>27-27</pages><artnum>27</artnum><issn>0032-0935</issn><issn>1432-2048</issn><eissn>1432-2048</eissn><abstract>Main conclusion
In
Brassica rapa
, the epigenetic modifier BraA.CLF orchestrates flowering by modulating H3K27me3 levels at the floral integrator genes
FT
,
SOC1
, and
SEP3
, thereby influencing their expression.
CURLY LEAF (CLF) is the catalytic subunit of the plant Polycomb Repressive Complex 2 that mediates the trimethylation of histone H3 lysine 27 (H3K27me3), an epigenetic modification that leads to gene silencing. While the function of CURLY LEAF (CLF) has been extensively studied in
Arabidopsis thaliana
, its role in Brassica crops is barely known. In this study, we focused on the
Brassica rapa
homolog of
CLF
and found that the loss-of-function mutant
braA.clf-1
exhibits an accelerated flowering together with pleiotropic phenotypic alterations compared to wild-type plants. In addition, we carried out transcriptomic and H3K27me3 genome-wide analyses to identify the genes regulated by BraA.CLF. Interestingly, we observed that several floral regulatory genes, including the
B. rapa
homologs of
FT
,
SOC1
and
SEP3
, show reduced H3K27me3 levels and increased transcript levels compared to wild-type plants, suggesting that they are direct targets of BraA.CLF and key players in regulating flowering time in this crop. In addition, the results obtained will enhance our understanding of the epigenetic mechanisms regulating key developmental traits and will aid to increase crop yield by engineering new Brassica varieties with different flowering time requirements.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38865018</pmid><doi>10.1007/s00425-024-04454-7</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1276-9792</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 2024-07, Vol.260 (1), p.27-27, Article 27 |
| issn | 0032-0935 1432-2048 1432-2048 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Agriculture Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biomedical and Life Sciences Brassica Brassica rapa Brassica rapa - genetics Brassica rapa - growth & development Brassica rapa - physiology Crop yield Ecology Epigenesis, Genetic Epigenetics Flowering Flowers - genetics Flowers - growth & development Flowers - physiology Forestry Gene Expression Regulation, Plant Gene silencing Genes Histone H3 Histone-Lysine N-Methyltransferase - genetics Histone-Lysine N-Methyltransferase - metabolism Histones Histones - genetics Histones - metabolism Leaves Life Sciences loss-of-function mutation Lysine Methylation Methyltransferase methyltransferases mutants New varieties Original Original Article phenotype Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences Plants (botany) Polycomb group proteins protein subunits Transcriptomics |
| title | Brassica rapa CURLY LEAF is a major H3K27 methyltransferase regulating flowering time |
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