Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma
Expression of the Wilms' tumor gene WT1 was examined in 59 cases of colorectal adenocarcinoma to examine the involvement of WT1 in tumorigenesis. Quantitative real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) showed that WT1 mRNA was expressed in the range from 7.2x10−5 to 4.9x...
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| Veröffentlicht in: | Cancer science 2003-08, Vol.94 (8), p.712-717 |
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| creator | Oji, Yusuke Yamamoto, Hirofumi Nomura, Masaya Nakano, Yoko Ikeba, Ai Nakatsuka, Shin‐ichi Abeno, Sakie Kiyotoh, Eiji Jomgeow, Tanyarat Sekimoto, Mitsugu Nezu, Riichiro Yoshikawa, Yukinobu Inoue, Yoshifumi Hosen, Naoki Kawakami, Manabu Tsuboi, Akihiro Oka, Yoshihiro Ogawa, Hiroyasu Souda, Shigeo Aozasa, Katsuyuki Monden, Morito Sugiyama, Haruo |
| description | Expression of the Wilms' tumor gene WT1 was examined in 59 cases of colorectal adenocarcinoma to examine the involvement of WT1 in tumorigenesis. Quantitative real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) showed that WT1 mRNA was expressed in the range from 7.2x10−5 to 4.9x10−1 levels (WT1 expression level in K562 leukemic cells was defined as 1.0) in all (100%) of the 28 cases of colorectal adenocarcinoma examined, and that the WT1 mRNA expression levels were higher in 20 (71%) of the 28 cases compared to those of normal‐appearing mucosal tissues examined. Immunohistochemical analysis using an anti‐WT1 antibody was performed on 46 cases of colorectal adenocarcinoma (15 of the 28 cases with WT1 mRNA expression and 31 newly collected cases), and the expression of WT1 protein was detected in 41 (89%) of the 46 cases. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of the 10 exons of the WT1 gene in any of 5 different colorectal ade‐nocarcinomas. These results may indicate an important role of the wild‐type WT1 gene in tumorigenesis of colorectal adenocarcinoma. |
| doi_str_mv | 10.1111/j.1349-7006.2003.tb01507.x |
| format | Article |
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Quantitative real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) showed that WT1 mRNA was expressed in the range from 7.2x10−5 to 4.9x10−1 levels (WT1 expression level in K562 leukemic cells was defined as 1.0) in all (100%) of the 28 cases of colorectal adenocarcinoma examined, and that the WT1 mRNA expression levels were higher in 20 (71%) of the 28 cases compared to those of normal‐appearing mucosal tissues examined. Immunohistochemical analysis using an anti‐WT1 antibody was performed on 46 cases of colorectal adenocarcinoma (15 of the 28 cases with WT1 mRNA expression and 31 newly collected cases), and the expression of WT1 protein was detected in 41 (89%) of the 46 cases. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of the 10 exons of the WT1 gene in any of 5 different colorectal ade‐nocarcinomas. These results may indicate an important role of the wild‐type WT1 gene in tumorigenesis of colorectal adenocarcinoma.</description><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>DOI: 10.1111/j.1349-7006.2003.tb01507.x</identifier><identifier>PMID: 12901797</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenocarcinoma ; Adenocarcinoma - genetics ; Adenocarcinoma - pathology ; Adult ; Aged ; Aged, 80 and over ; Base Sequence ; Biological and medical sciences ; Colonic Neoplasms - genetics ; Colonic Neoplasms - pathology ; DNA Primers ; DNA sequencing ; Exons ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic - genetics ; Genes, Wilms Tumor ; Genomics ; Humans ; Leukemia ; Male ; Medical sciences ; Middle Aged ; Mucosa ; Neoplasm Staging ; Polymerase chain reaction ; Rectal Neoplasms - genetics ; Rectal Neoplasms - pathology ; Reverse Transcriptase Polymerase Chain Reaction ; Reverse transcription ; Sequence analysis ; Tumorigenesis ; Tumors ; Wilms' tumor ; WT1 protein ; WT1 Proteins - genetics</subject><ispartof>Cancer science, 2003-08, Vol.94 (8), p.712-717</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright John Wiley & Sons, Inc. Aug 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6592-38a83ad225b13d4d9a3c584e462c6a84ff05bbe0ee38ac10a1d04cd78907b65e3</citedby><cites>FETCH-LOGICAL-c6592-38a83ad225b13d4d9a3c584e462c6a84ff05bbe0ee38ac10a1d04cd78907b65e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11160268/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11160268/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1349-7006.2003.tb01507.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15368994$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12901797$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oji, Yusuke</creatorcontrib><creatorcontrib>Yamamoto, Hirofumi</creatorcontrib><creatorcontrib>Nomura, Masaya</creatorcontrib><creatorcontrib>Nakano, Yoko</creatorcontrib><creatorcontrib>Ikeba, Ai</creatorcontrib><creatorcontrib>Nakatsuka, Shin‐ichi</creatorcontrib><creatorcontrib>Abeno, Sakie</creatorcontrib><creatorcontrib>Kiyotoh, Eiji</creatorcontrib><creatorcontrib>Jomgeow, Tanyarat</creatorcontrib><creatorcontrib>Sekimoto, Mitsugu</creatorcontrib><creatorcontrib>Nezu, Riichiro</creatorcontrib><creatorcontrib>Yoshikawa, Yukinobu</creatorcontrib><creatorcontrib>Inoue, Yoshifumi</creatorcontrib><creatorcontrib>Hosen, Naoki</creatorcontrib><creatorcontrib>Kawakami, Manabu</creatorcontrib><creatorcontrib>Tsuboi, Akihiro</creatorcontrib><creatorcontrib>Oka, Yoshihiro</creatorcontrib><creatorcontrib>Ogawa, Hiroyasu</creatorcontrib><creatorcontrib>Souda, Shigeo</creatorcontrib><creatorcontrib>Aozasa, Katsuyuki</creatorcontrib><creatorcontrib>Monden, Morito</creatorcontrib><creatorcontrib>Sugiyama, Haruo</creatorcontrib><title>Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma</title><title>Cancer science</title><addtitle>Cancer Sci</addtitle><description>Expression of the Wilms' tumor gene WT1 was examined in 59 cases of colorectal adenocarcinoma to examine the involvement of WT1 in tumorigenesis. Quantitative real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) showed that WT1 mRNA was expressed in the range from 7.2x10−5 to 4.9x10−1 levels (WT1 expression level in K562 leukemic cells was defined as 1.0) in all (100%) of the 28 cases of colorectal adenocarcinoma examined, and that the WT1 mRNA expression levels were higher in 20 (71%) of the 28 cases compared to those of normal‐appearing mucosal tissues examined. Immunohistochemical analysis using an anti‐WT1 antibody was performed on 46 cases of colorectal adenocarcinoma (15 of the 28 cases with WT1 mRNA expression and 31 newly collected cases), and the expression of WT1 protein was detected in 41 (89%) of the 46 cases. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of the 10 exons of the WT1 gene in any of 5 different colorectal ade‐nocarcinomas. These results may indicate an important role of the wild‐type WT1 gene in tumorigenesis of colorectal adenocarcinoma.</description><subject>Adenocarcinoma</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - pathology</subject><subject>DNA Primers</subject><subject>DNA sequencing</subject><subject>Exons</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Genes, Wilms Tumor</subject><subject>Genomics</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mucosa</subject><subject>Neoplasm Staging</subject><subject>Polymerase chain reaction</subject><subject>Rectal Neoplasms - genetics</subject><subject>Rectal Neoplasms - pathology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Reverse transcription</subject><subject>Sequence analysis</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>Wilms' tumor</subject><subject>WT1 protein</subject><subject>WT1 Proteins - genetics</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkU9v1DAQxSMEoqXwFVAEopwS_Ce2Yw6gagUUqVIPbcXRcpxJ61Vib-2k3X57HDZqSy8IX2zN_ObpeV6WvcOoxOl8WpeYVrIQCPGSIETLsUGYIVFun2X7963nf96ikIiSvexVjOuE8kpWL7M9TCTCQor97Pj0BgJsNwFitN7lvsvHK8h_2X6IH_NxGnzIL8GlyjnOrcuN730AM-o-1y04b3Qw1vlBv85edLqP8Ga5D7KL79_OV8fFyemPn6ujk8JwJklBa11T3RLCGkzbqpWaGlZXUHFiuK6rrkOsaQABJNJgpHGLKtOKWiLRcAb0IPu6091MzQCtATcG3atNsIMOd8prq_7uOHulLv2NSpvjiPA6KRwuCsFfTxBHNdhooO-1Az9FJSgjjIrqnyCWhHHBcALfPwHXfgourUERKmWNEapnuc87ygQfY4Du3jRGszus1mpOT83pqTlYtQSrtmn47eNvP4wuSSbgwwLoaHTfBe2MjQ8co7yWcnbxZcfd2h7u_sOCWh2dCUzobx8Rv8E</recordid><startdate>200308</startdate><enddate>200308</enddate><creator>Oji, Yusuke</creator><creator>Yamamoto, Hirofumi</creator><creator>Nomura, Masaya</creator><creator>Nakano, Yoko</creator><creator>Ikeba, Ai</creator><creator>Nakatsuka, Shin‐ichi</creator><creator>Abeno, Sakie</creator><creator>Kiyotoh, Eiji</creator><creator>Jomgeow, Tanyarat</creator><creator>Sekimoto, Mitsugu</creator><creator>Nezu, Riichiro</creator><creator>Yoshikawa, Yukinobu</creator><creator>Inoue, Yoshifumi</creator><creator>Hosen, Naoki</creator><creator>Kawakami, Manabu</creator><creator>Tsuboi, Akihiro</creator><creator>Oka, Yoshihiro</creator><creator>Ogawa, Hiroyasu</creator><creator>Souda, Shigeo</creator><creator>Aozasa, Katsuyuki</creator><creator>Monden, Morito</creator><creator>Sugiyama, Haruo</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>John Wiley & Sons, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200308</creationdate><title>Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma</title><author>Oji, Yusuke ; Yamamoto, Hirofumi ; Nomura, Masaya ; Nakano, Yoko ; Ikeba, Ai ; Nakatsuka, Shin‐ichi ; Abeno, Sakie ; Kiyotoh, Eiji ; Jomgeow, Tanyarat ; Sekimoto, Mitsugu ; Nezu, Riichiro ; Yoshikawa, Yukinobu ; Inoue, Yoshifumi ; Hosen, Naoki ; Kawakami, Manabu ; Tsuboi, Akihiro ; Oka, Yoshihiro ; Ogawa, Hiroyasu ; Souda, Shigeo ; Aozasa, Katsuyuki ; Monden, Morito ; Sugiyama, Haruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6592-38a83ad225b13d4d9a3c584e462c6a84ff05bbe0ee38ac10a1d04cd78907b65e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adenocarcinoma</topic><topic>Adenocarcinoma - 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genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oji, Yusuke</creatorcontrib><creatorcontrib>Yamamoto, Hirofumi</creatorcontrib><creatorcontrib>Nomura, Masaya</creatorcontrib><creatorcontrib>Nakano, Yoko</creatorcontrib><creatorcontrib>Ikeba, Ai</creatorcontrib><creatorcontrib>Nakatsuka, Shin‐ichi</creatorcontrib><creatorcontrib>Abeno, Sakie</creatorcontrib><creatorcontrib>Kiyotoh, Eiji</creatorcontrib><creatorcontrib>Jomgeow, Tanyarat</creatorcontrib><creatorcontrib>Sekimoto, Mitsugu</creatorcontrib><creatorcontrib>Nezu, Riichiro</creatorcontrib><creatorcontrib>Yoshikawa, Yukinobu</creatorcontrib><creatorcontrib>Inoue, Yoshifumi</creatorcontrib><creatorcontrib>Hosen, Naoki</creatorcontrib><creatorcontrib>Kawakami, Manabu</creatorcontrib><creatorcontrib>Tsuboi, Akihiro</creatorcontrib><creatorcontrib>Oka, Yoshihiro</creatorcontrib><creatorcontrib>Ogawa, Hiroyasu</creatorcontrib><creatorcontrib>Souda, Shigeo</creatorcontrib><creatorcontrib>Aozasa, Katsuyuki</creatorcontrib><creatorcontrib>Monden, Morito</creatorcontrib><creatorcontrib>Sugiyama, Haruo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Oji, Yusuke</au><au>Yamamoto, Hirofumi</au><au>Nomura, Masaya</au><au>Nakano, Yoko</au><au>Ikeba, Ai</au><au>Nakatsuka, Shin‐ichi</au><au>Abeno, Sakie</au><au>Kiyotoh, Eiji</au><au>Jomgeow, Tanyarat</au><au>Sekimoto, Mitsugu</au><au>Nezu, Riichiro</au><au>Yoshikawa, Yukinobu</au><au>Inoue, Yoshifumi</au><au>Hosen, Naoki</au><au>Kawakami, Manabu</au><au>Tsuboi, Akihiro</au><au>Oka, Yoshihiro</au><au>Ogawa, Hiroyasu</au><au>Souda, Shigeo</au><au>Aozasa, Katsuyuki</au><au>Monden, Morito</au><au>Sugiyama, Haruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2003-08</date><risdate>2003</risdate><volume>94</volume><issue>8</issue><spage>712</spage><epage>717</epage><pages>712-717</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>Expression of the Wilms' tumor gene WT1 was examined in 59 cases of colorectal adenocarcinoma to examine the involvement of WT1 in tumorigenesis. Quantitative real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) showed that WT1 mRNA was expressed in the range from 7.2x10−5 to 4.9x10−1 levels (WT1 expression level in K562 leukemic cells was defined as 1.0) in all (100%) of the 28 cases of colorectal adenocarcinoma examined, and that the WT1 mRNA expression levels were higher in 20 (71%) of the 28 cases compared to those of normal‐appearing mucosal tissues examined. Immunohistochemical analysis using an anti‐WT1 antibody was performed on 46 cases of colorectal adenocarcinoma (15 of the 28 cases with WT1 mRNA expression and 31 newly collected cases), and the expression of WT1 protein was detected in 41 (89%) of the 46 cases. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of the 10 exons of the WT1 gene in any of 5 different colorectal ade‐nocarcinomas. These results may indicate an important role of the wild‐type WT1 gene in tumorigenesis of colorectal adenocarcinoma.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>12901797</pmid><doi>10.1111/j.1349-7006.2003.tb01507.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cancer science, 2003-08, Vol.94 (8), p.712-717 |
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| source | Wiley Online Library Open Access |
| subjects | Adenocarcinoma Adenocarcinoma - genetics Adenocarcinoma - pathology Adult Aged Aged, 80 and over Base Sequence Biological and medical sciences Colonic Neoplasms - genetics Colonic Neoplasms - pathology DNA Primers DNA sequencing Exons Female Gene expression Gene Expression Regulation, Neoplastic - genetics Genes, Wilms Tumor Genomics Humans Leukemia Male Medical sciences Middle Aged Mucosa Neoplasm Staging Polymerase chain reaction Rectal Neoplasms - genetics Rectal Neoplasms - pathology Reverse Transcriptase Polymerase Chain Reaction Reverse transcription Sequence analysis Tumorigenesis Tumors Wilms' tumor WT1 protein WT1 Proteins - genetics |
| title | Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma |
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