Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development
Atypical adenomatous hyperplasia (AAH) is classified as a precancerous lesion of lung adenocarcinoma. We established an immortalized AAH cell line (PL16T) and a human non‐neoplastic bronchial epithelial cell line (PL16B) from the same patient by transfection with the gene for SV40 large T antigen. T...
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| Veröffentlicht in: | Cancer science 2005-10, Vol.96 (10), p.668-675 |
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| creator | Shimada, Aki Kano, Junko Ishiyama, Tadashi Okubo, Chigusa Iijima, Tatsuo Morishita, Yukio Minami, Yuko Inadome, Yukinori Shu, Yujian Sugita, Shintaro Takeuchi, Tomoyo Noguchi, Masayuki |
| description | Atypical adenomatous hyperplasia (AAH) is classified as a precancerous lesion of lung adenocarcinoma. We established an immortalized AAH cell line (PL16T) and a human non‐neoplastic bronchial epithelial cell line (PL16B) from the same patient by transfection with the gene for SV40 large T antigen. The expression profile of PL16T was compared with that of PL16B by the suppression subtractive hybridization method. From 704 selectively hybridized clones, we finally selected 25 fragments of mRNA that showed transcription levels more than three times higher in PL16T than in PL16B. Thirteen (52%) and eight (32%) of them encoded tumor‐associated calcium signal transducer 2 (TACSTD2) and S100 calcium binding protein A2 (S100A2), respectively. The high transcription of TACSTD2 and S100A2 in PL16T was confirmed by in situ hybridization. In normal lung tissue, both TACSTD2 and S100A2 were expressed at very low levels, but seven and five of 14 AAH were positive for TACSTD2 and S100A2, respectively. The frequency of TACSTD2 positivity was increased in 16 of 22 bronchioloalveolar carcinomas (BAC) and adenocarcinoma with mixed subtype with BAC component (mixed BAC). Positivity for S100A2 occurred in four of 22 BAC and mixed BAC. The abnormal transcription of TACSTD2 and S100A2 are thought to be unique molecular markers of the preinvasive stage of lung adenocarcinoma.(Cancer Sci 2005; 96: 668 – 675) |
| doi_str_mv | 10.1111/j.1349-7006.2005.00100.x |
| format | Article |
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We established an immortalized AAH cell line (PL16T) and a human non‐neoplastic bronchial epithelial cell line (PL16B) from the same patient by transfection with the gene for SV40 large T antigen. The expression profile of PL16T was compared with that of PL16B by the suppression subtractive hybridization method. From 704 selectively hybridized clones, we finally selected 25 fragments of mRNA that showed transcription levels more than three times higher in PL16T than in PL16B. Thirteen (52%) and eight (32%) of them encoded tumor‐associated calcium signal transducer 2 (TACSTD2) and S100 calcium binding protein A2 (S100A2), respectively. The high transcription of TACSTD2 and S100A2 in PL16T was confirmed by in situ hybridization. In normal lung tissue, both TACSTD2 and S100A2 were expressed at very low levels, but seven and five of 14 AAH were positive for TACSTD2 and S100A2, respectively. The frequency of TACSTD2 positivity was increased in 16 of 22 bronchioloalveolar carcinomas (BAC) and adenocarcinoma with mixed subtype with BAC component (mixed BAC). Positivity for S100A2 occurred in four of 22 BAC and mixed BAC. The abnormal transcription of TACSTD2 and S100A2 are thought to be unique molecular markers of the preinvasive stage of lung adenocarcinoma.(Cancer Sci 2005; 96: 668 – 675)</description><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>DOI: 10.1111/j.1349-7006.2005.00100.x</identifier><identifier>PMID: 16232198</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Pty</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - pathology ; Antigens, Neoplasm - analysis ; Antigens, Neoplasm - biosynthesis ; Antigens, Neoplasm - genetics ; Biological and medical sciences ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - pathology ; Cell Adhesion Molecules - analysis ; Cell Adhesion Molecules - biosynthesis ; Cell Adhesion Molecules - genetics ; Chemotactic Factors - analysis ; Chemotactic Factors - biosynthesis ; Chemotactic Factors - genetics ; Female ; Gene Expression Profiling ; Humans ; Hyperplasia ; Lung - pathology ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Medical sciences ; Middle Aged ; Neoplasm Staging ; Nucleic Acid Hybridization ; Original ; Pneumology ; Precancerous Conditions - genetics ; Precancerous Conditions - pathology ; S100 Proteins - analysis ; S100 Proteins - biosynthesis ; S100 Proteins - genetics ; Simian virus 40 ; Tumor Cells, Cultured ; Tumors ; Tumors of the respiratory system and mediastinum</subject><ispartof>Cancer science, 2005-10, Vol.96 (10), p.668-675</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6520-8a8f5a79968323aff4b4b5fece5e5169386a71df700d44b2fbf5cc932b6fccea3</citedby><cites>FETCH-LOGICAL-c6520-8a8f5a79968323aff4b4b5fece5e5169386a71df700d44b2fbf5cc932b6fccea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11159238/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11159238/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1349-7006.2005.00100.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17267723$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16232198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimada, Aki</creatorcontrib><creatorcontrib>Kano, Junko</creatorcontrib><creatorcontrib>Ishiyama, Tadashi</creatorcontrib><creatorcontrib>Okubo, Chigusa</creatorcontrib><creatorcontrib>Iijima, Tatsuo</creatorcontrib><creatorcontrib>Morishita, Yukio</creatorcontrib><creatorcontrib>Minami, Yuko</creatorcontrib><creatorcontrib>Inadome, Yukinori</creatorcontrib><creatorcontrib>Shu, Yujian</creatorcontrib><creatorcontrib>Sugita, Shintaro</creatorcontrib><creatorcontrib>Takeuchi, Tomoyo</creatorcontrib><creatorcontrib>Noguchi, Masayuki</creatorcontrib><title>Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development</title><title>Cancer science</title><addtitle>Cancer Sci</addtitle><description>Atypical adenomatous hyperplasia (AAH) is classified as a precancerous lesion of lung adenocarcinoma. We established an immortalized AAH cell line (PL16T) and a human non‐neoplastic bronchial epithelial cell line (PL16B) from the same patient by transfection with the gene for SV40 large T antigen. The expression profile of PL16T was compared with that of PL16B by the suppression subtractive hybridization method. From 704 selectively hybridized clones, we finally selected 25 fragments of mRNA that showed transcription levels more than three times higher in PL16T than in PL16B. Thirteen (52%) and eight (32%) of them encoded tumor‐associated calcium signal transducer 2 (TACSTD2) and S100 calcium binding protein A2 (S100A2), respectively. The high transcription of TACSTD2 and S100A2 in PL16T was confirmed by in situ hybridization. In normal lung tissue, both TACSTD2 and S100A2 were expressed at very low levels, but seven and five of 14 AAH were positive for TACSTD2 and S100A2, respectively. The frequency of TACSTD2 positivity was increased in 16 of 22 bronchioloalveolar carcinomas (BAC) and adenocarcinoma with mixed subtype with BAC component (mixed BAC). Positivity for S100A2 occurred in four of 22 BAC and mixed BAC. The abnormal transcription of TACSTD2 and S100A2 are thought to be unique molecular markers of the preinvasive stage of lung adenocarcinoma.(Cancer Sci 2005; 96: 668 – 675)</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - pathology</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Neoplasm - biosynthesis</subject><subject>Antigens, Neoplasm - genetics</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Cell Adhesion Molecules - biosynthesis</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Chemotactic Factors - analysis</subject><subject>Chemotactic Factors - biosynthesis</subject><subject>Chemotactic Factors - genetics</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Hyperplasia</subject><subject>Lung - pathology</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Nucleic Acid Hybridization</subject><subject>Original</subject><subject>Pneumology</subject><subject>Precancerous Conditions - genetics</subject><subject>Precancerous Conditions - pathology</subject><subject>S100 Proteins - analysis</subject><subject>S100 Proteins - biosynthesis</subject><subject>S100 Proteins - genetics</subject><subject>Simian virus 40</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt-LEzEQxxdRvPP0X5C86JOt-bGb3YAgRzl_wIEP6nOYzU7alGxSk_Zs_af8F81ey52CoHnJkPnMdyYzU1WE0Tkr5_V6zkStZi2lcs4pbeaUMkrn-wfV-Z3j4a3dzhQV_Kx6kvOaUiFrVT-uzpjkgjPVnVc_r_IWeu_yasSwJdESCMSNY0xb8O4HDsSg98S7gMSmOBIgm4QGgsEUd5l4zC6GKc7vwpLAgCEaSMaFOMKrIjaQJQbMZOWWK38guC_hORddF8h2hQQhledSxLJAf5chA96gj5upwqfVIws-47PTfVF9fXf1ZfFhdv3p_cfF5fXMyIbTWQedbaBVSnaCC7C27uu-sWiwwYZJJToJLRtsadRQ1z23vW2MUYL30hqDIC6qt0fdza4fcTAldQKvN8mNkA46gtN_eoJb6WW80WU8jeKiKwovTwopftth3urR5amZELB0TstOKqWa5p8gZ6xlXLECdkfQpJhzQntXDqNTXqbXehq_nsavp73Qt3uh9yX0-e_fuQ88LUIBXpwAyAa8TWXCLt9zLZdty0Xh3hy5787j4b8L0IvLz8UQvwDItdlR</recordid><startdate>200510</startdate><enddate>200510</enddate><creator>Shimada, Aki</creator><creator>Kano, Junko</creator><creator>Ishiyama, Tadashi</creator><creator>Okubo, Chigusa</creator><creator>Iijima, Tatsuo</creator><creator>Morishita, Yukio</creator><creator>Minami, Yuko</creator><creator>Inadome, Yukinori</creator><creator>Shu, Yujian</creator><creator>Sugita, Shintaro</creator><creator>Takeuchi, Tomoyo</creator><creator>Noguchi, Masayuki</creator><general>Blackwell Science Pty</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200510</creationdate><title>Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development</title><author>Shimada, Aki ; Kano, Junko ; Ishiyama, Tadashi ; Okubo, Chigusa ; Iijima, Tatsuo ; Morishita, Yukio ; Minami, Yuko ; Inadome, Yukinori ; Shu, Yujian ; Sugita, Shintaro ; Takeuchi, Tomoyo ; Noguchi, Masayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6520-8a8f5a79968323aff4b4b5fece5e5169386a71df700d44b2fbf5cc932b6fccea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - pathology</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Neoplasm - biosynthesis</topic><topic>Antigens, Neoplasm - genetics</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Chemotactic Factors - analysis</topic><topic>Chemotactic Factors - biosynthesis</topic><topic>Chemotactic Factors - genetics</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Hyperplasia</topic><topic>Lung - pathology</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>Nucleic Acid Hybridization</topic><topic>Original</topic><topic>Pneumology</topic><topic>Precancerous Conditions - genetics</topic><topic>Precancerous Conditions - pathology</topic><topic>S100 Proteins - analysis</topic><topic>S100 Proteins - biosynthesis</topic><topic>S100 Proteins - genetics</topic><topic>Simian virus 40</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimada, Aki</creatorcontrib><creatorcontrib>Kano, Junko</creatorcontrib><creatorcontrib>Ishiyama, Tadashi</creatorcontrib><creatorcontrib>Okubo, Chigusa</creatorcontrib><creatorcontrib>Iijima, Tatsuo</creatorcontrib><creatorcontrib>Morishita, Yukio</creatorcontrib><creatorcontrib>Minami, Yuko</creatorcontrib><creatorcontrib>Inadome, Yukinori</creatorcontrib><creatorcontrib>Shu, Yujian</creatorcontrib><creatorcontrib>Sugita, Shintaro</creatorcontrib><creatorcontrib>Takeuchi, Tomoyo</creatorcontrib><creatorcontrib>Noguchi, Masayuki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Shimada, Aki</au><au>Kano, Junko</au><au>Ishiyama, Tadashi</au><au>Okubo, Chigusa</au><au>Iijima, Tatsuo</au><au>Morishita, Yukio</au><au>Minami, Yuko</au><au>Inadome, Yukinori</au><au>Shu, Yujian</au><au>Sugita, Shintaro</au><au>Takeuchi, Tomoyo</au><au>Noguchi, Masayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2005-10</date><risdate>2005</risdate><volume>96</volume><issue>10</issue><spage>668</spage><epage>675</epage><pages>668-675</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>Atypical adenomatous hyperplasia (AAH) is classified as a precancerous lesion of lung adenocarcinoma. We established an immortalized AAH cell line (PL16T) and a human non‐neoplastic bronchial epithelial cell line (PL16B) from the same patient by transfection with the gene for SV40 large T antigen. The expression profile of PL16T was compared with that of PL16B by the suppression subtractive hybridization method. From 704 selectively hybridized clones, we finally selected 25 fragments of mRNA that showed transcription levels more than three times higher in PL16T than in PL16B. Thirteen (52%) and eight (32%) of them encoded tumor‐associated calcium signal transducer 2 (TACSTD2) and S100 calcium binding protein A2 (S100A2), respectively. The high transcription of TACSTD2 and S100A2 in PL16T was confirmed by in situ hybridization. In normal lung tissue, both TACSTD2 and S100A2 were expressed at very low levels, but seven and five of 14 AAH were positive for TACSTD2 and S100A2, respectively. The frequency of TACSTD2 positivity was increased in 16 of 22 bronchioloalveolar carcinomas (BAC) and adenocarcinoma with mixed subtype with BAC component (mixed BAC). Positivity for S100A2 occurred in four of 22 BAC and mixed BAC. The abnormal transcription of TACSTD2 and S100A2 are thought to be unique molecular markers of the preinvasive stage of lung adenocarcinoma.(Cancer Sci 2005; 96: 668 – 675)</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Pty</pub><pmid>16232198</pmid><doi>10.1111/j.1349-7006.2005.00100.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - pathology Antigens, Neoplasm - analysis Antigens, Neoplasm - biosynthesis Antigens, Neoplasm - genetics Biological and medical sciences Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - pathology Cell Adhesion Molecules - analysis Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - genetics Chemotactic Factors - analysis Chemotactic Factors - biosynthesis Chemotactic Factors - genetics Female Gene Expression Profiling Humans Hyperplasia Lung - pathology Lung Neoplasms - genetics Lung Neoplasms - pathology Medical sciences Middle Aged Neoplasm Staging Nucleic Acid Hybridization Original Pneumology Precancerous Conditions - genetics Precancerous Conditions - pathology S100 Proteins - analysis S100 Proteins - biosynthesis S100 Proteins - genetics Simian virus 40 Tumor Cells, Cultured Tumors Tumors of the respiratory system and mediastinum |
| title | Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development |
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