Aberrant maspin expression in human endometrial cancer
Maspin, a mammary serine protease inhibitor, was originally reported as a tumor suppressor gene in breast cancer. The purpose of the present study was to examine maspin expression and evaluate its clinicopathological significance in endometrial cancer. We examined maspin expression immunohistochemic...
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| Veröffentlicht in: | Cancer science 2006-09, Vol.97 (9), p.883-888 |
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| creator | Murai, Shinya Maesawa, Chihaya Masuda, Tomoyuki Sugiyama, Toru |
| description | Maspin, a mammary serine protease inhibitor, was originally reported as a tumor suppressor gene in breast cancer. The purpose of the present study was to examine maspin expression and evaluate its clinicopathological significance in endometrial cancer. We examined maspin expression immunohistochemically in 41 cases with endometrioid adenocarcinoma. DNA methylation status at the maspin promoter region was determined by the methylation‐specific polymerase chain reaction method. Aberrant maspin expression was observed in 27 (66%) of 41 endometrioid adenocarcinomas but not in normal endometrial glands. Maspin immunoreactivity of the tumor cells varied in incidence and density among tumors. Positive staining was correlated significantly with the presence of squamous differentiation (presence vs absence = 11/11 [100%] vs 16/30 [53%], P |
| doi_str_mv | 10.1111/j.1349-7006.2006.00266.x |
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The purpose of the present study was to examine maspin expression and evaluate its clinicopathological significance in endometrial cancer. We examined maspin expression immunohistochemically in 41 cases with endometrioid adenocarcinoma. DNA methylation status at the maspin promoter region was determined by the methylation‐specific polymerase chain reaction method. Aberrant maspin expression was observed in 27 (66%) of 41 endometrioid adenocarcinomas but not in normal endometrial glands. Maspin immunoreactivity of the tumor cells varied in incidence and density among tumors. Positive staining was correlated significantly with the presence of squamous differentiation (presence vs absence = 11/11 [100%] vs 16/30 [53%], P < 0.05), and nuclear subcellular localization of maspin protein was also significantly associated with squamous differentiation (nuclear positive vs nuclear negative = 6/11 [54%] vs 2/30 [6.7%], P < 0.05). An inverse correlation between their immunoreactivity and methylation status was observed (P < 0.01). Three of the four cell lines established from endometrioid adenocarcinomas overexpressed maspin mRNA and its protein product. In a maspin‐negative cell line, maspin expression was induced by treatment with 5‐aza‐2′‐deoxycytidine, a DNA demethylating agent. There was no significant correlation between maspin expression and any clinicopathlogical data. These findings suggest that maspin induced by DNA demethylation at the promoter region may contribute to squamous differentiation of tumor cells in endometrioid adenocarcinomas. 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The purpose of the present study was to examine maspin expression and evaluate its clinicopathological significance in endometrial cancer. We examined maspin expression immunohistochemically in 41 cases with endometrioid adenocarcinoma. DNA methylation status at the maspin promoter region was determined by the methylation‐specific polymerase chain reaction method. Aberrant maspin expression was observed in 27 (66%) of 41 endometrioid adenocarcinomas but not in normal endometrial glands. Maspin immunoreactivity of the tumor cells varied in incidence and density among tumors. Positive staining was correlated significantly with the presence of squamous differentiation (presence vs absence = 11/11 [100%] vs 16/30 [53%], P < 0.05), and nuclear subcellular localization of maspin protein was also significantly associated with squamous differentiation (nuclear positive vs nuclear negative = 6/11 [54%] vs 2/30 [6.7%], P < 0.05). An inverse correlation between their immunoreactivity and methylation status was observed (P < 0.01). Three of the four cell lines established from endometrioid adenocarcinomas overexpressed maspin mRNA and its protein product. In a maspin‐negative cell line, maspin expression was induced by treatment with 5‐aza‐2′‐deoxycytidine, a DNA demethylating agent. There was no significant correlation between maspin expression and any clinicopathlogical data. These findings suggest that maspin induced by DNA demethylation at the promoter region may contribute to squamous differentiation of tumor cells in endometrioid adenocarcinomas. (Cancer Sci 2006; 97: 883–888)</description><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Line, Tumor</subject><subject>DNA Methylation</subject><subject>Endometrial Neoplasms - metabolism</subject><subject>Endometrial Neoplasms - pathology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Genes, Tumor Suppressor</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Original</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>Serpins - biosynthesis</subject><subject>Serpins - genetics</subject><subject>Tumors</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAQRS0EoqXwCygb2KX4kTixhISqipdUiQWwtiaOQ13lUewE2r_HaasWdnhhjz1nrq8uQgHBY-LXzWJMWCTCBGM-pv2GMeV8vDpCw33jeFMnocCMDtCZcwuMGY9EdIoGhKeUUsGHiE8ybS3UbVCBW5o60Kul1c6Zpg78bd5V4N_qvKl0aw2UgYJaaXuOTgoonb7YnSP0_nD_Nn0KZy-Pz9PJLFRxHPMQigyrnBGaA4-AJzmJ00RDliQcF4C1wIXSIspIInIliAAqCGPeJhEZzWLFRuhuq7vsskrnStethVIuranArmUDRv7t1GYuP5ov6VOK0yjCXuF6p2Cbz067VlbGKV2WUOumc5Kn3kscMQ-mW1DZxjmri_0vBPdyRC5kH67sw5V96nKTulz50cvfLg-Du5g9cLUDwCkoCx-4Mu7ApThNSNpzt1vu25R6_W8Dcjp59QX7AYqEnfw</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>Murai, Shinya</creator><creator>Maesawa, Chihaya</creator><creator>Masuda, Tomoyuki</creator><creator>Sugiyama, Toru</creator><general>Blackwell Publishing Asia</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200609</creationdate><title>Aberrant maspin expression in human endometrial cancer</title><author>Murai, Shinya ; Maesawa, Chihaya ; Masuda, Tomoyuki ; Sugiyama, Toru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5556-afb0cd312da64a67d1587eab7760fa0e90fce94b179dc919a2913303619b2b5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Line, Tumor</topic><topic>DNA Methylation</topic><topic>Endometrial Neoplasms - metabolism</topic><topic>Endometrial Neoplasms - pathology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Genes, Tumor Suppressor</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Original</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Serpins - biosynthesis</topic><topic>Serpins - genetics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murai, Shinya</creatorcontrib><creatorcontrib>Maesawa, Chihaya</creatorcontrib><creatorcontrib>Masuda, Tomoyuki</creatorcontrib><creatorcontrib>Sugiyama, Toru</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Murai, Shinya</au><au>Maesawa, Chihaya</au><au>Masuda, Tomoyuki</au><au>Sugiyama, Toru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aberrant maspin expression in human endometrial cancer</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2006-09</date><risdate>2006</risdate><volume>97</volume><issue>9</issue><spage>883</spage><epage>888</epage><pages>883-888</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>Maspin, a mammary serine protease inhibitor, was originally reported as a tumor suppressor gene in breast cancer. The purpose of the present study was to examine maspin expression and evaluate its clinicopathological significance in endometrial cancer. We examined maspin expression immunohistochemically in 41 cases with endometrioid adenocarcinoma. DNA methylation status at the maspin promoter region was determined by the methylation‐specific polymerase chain reaction method. Aberrant maspin expression was observed in 27 (66%) of 41 endometrioid adenocarcinomas but not in normal endometrial glands. Maspin immunoreactivity of the tumor cells varied in incidence and density among tumors. Positive staining was correlated significantly with the presence of squamous differentiation (presence vs absence = 11/11 [100%] vs 16/30 [53%], P < 0.05), and nuclear subcellular localization of maspin protein was also significantly associated with squamous differentiation (nuclear positive vs nuclear negative = 6/11 [54%] vs 2/30 [6.7%], P < 0.05). An inverse correlation between their immunoreactivity and methylation status was observed (P < 0.01). Three of the four cell lines established from endometrioid adenocarcinomas overexpressed maspin mRNA and its protein product. In a maspin‐negative cell line, maspin expression was induced by treatment with 5‐aza‐2′‐deoxycytidine, a DNA demethylating agent. There was no significant correlation between maspin expression and any clinicopathlogical data. These findings suggest that maspin induced by DNA demethylation at the promoter region may contribute to squamous differentiation of tumor cells in endometrioid adenocarcinomas. (Cancer Sci 2006; 97: 883–888)</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>16822296</pmid><doi>10.1111/j.1349-7006.2006.00266.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma - metabolism Adenocarcinoma - pathology Biological and medical sciences Blotting, Western Cell Line, Tumor DNA Methylation Endometrial Neoplasms - metabolism Endometrial Neoplasms - pathology Female Female genital diseases Genes, Tumor Suppressor Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Medical sciences Original Polymerase Chain Reaction Promoter Regions, Genetic - genetics RNA, Messenger - analysis Serpins - biosynthesis Serpins - genetics Tumors |
| title | Aberrant maspin expression in human endometrial cancer |
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