Mixed-phase weak anion-exchange/reversed-phase LC–MS/MS for analysis of nucleotide sugars in human fibroblasts
Nucleotide sugars (NS) fulfil important roles in all living organisms and in humans, related defects result in severe clinical syndromes. NS can be seen as the “activated” sugars used for biosynthesis of a wide range of glycoconjugates and serve as substrates themselves for the synthesis of other nu...
Gespeichert in:
Veröffentlicht in: | Analytical and bioanalytical chemistry 2024-06, Vol.416 (15), p.3595-3604 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 3604 |
---|---|
container_issue | 15 |
container_start_page | 3595 |
container_title | Analytical and bioanalytical chemistry |
container_volume | 416 |
creator | Rahm, Moritz Kwast, Hanneke Wessels, Hans J. C. T. Noga, Marek J. Lefeber, Dirk J. |
description | Nucleotide sugars (NS) fulfil important roles in all living organisms and in humans, related defects result in severe clinical syndromes. NS can be seen as the “activated” sugars used for biosynthesis of a wide range of glycoconjugates and serve as substrates themselves for the synthesis of other nucleotide sugars. NS analysis is complicated by the presence of multiple stereoisomers without diagnostic transition ions, therefore requiring separation by liquid chromatography. In this paper, we explored weak anion-exchange/reversed-phase chromatography on a hybrid column for the separation of 17 nucleotide sugars that can occur in humans. A robust and reproducible method was established with intra- and inter-day coefficients of variation below 10% and a linear range spanning three orders of magnitude. Application to patient fibroblasts with genetic defects in mannose-1-phosphate guanylyltransferase beta, CDP-
l
-ribitol pyrophosphorylase A, and UDP-
N
-acetylglucosamine 2-epimerase/
N
-acetylmannosamine kinase showed abnormal levels of guanosine-5′-diphosphate-α-
d
-mannose (GDP-Man), cytidine-5′-diphosphate-
l
-ribitol (CDP-ribitol), and cytidine-5′-monophosphate-
N
-acetyl-β-
d
-neuraminic acid (CMP-Neu5Ac), respectively, in consonance with expectations based on the diagnosis. In conclusion, a novel, semi-quantitative method was established for the analysis of nucleotide sugars that can be applied to diagnose several genetic glycosylation disorders in fibroblasts and beyond. |
doi_str_mv | 10.1007/s00216-024-05313-w |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11156716</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3065093731</sourcerecordid><originalsourceid>FETCH-LOGICAL-c426t-5f37383063770b369bf0b6b9b361cb5c2b897bacc56c75c0c608f1f60e96e0243</originalsourceid><addsrcrecordid>eNp9kctuEzEUhi1ERS_wAiyQJTZshvgyY8-sEIpKQUrURWFt2c5x4jKxgz3TtDvegTfkSXBIGwoLVudI5zv_ufwIvaTkLSVETjIhjIqKsLoiDae82j5BJ1TQtmKiIU8Pec2O0WnO14TQpqXiGTrmrZCiZfwEbeb-FhbVZqUz4C3or1gHH0MFt3alwxImCW4g5QMym_78_mN-NZlfYRdTgXV_l33G0eEw2h7i4BeA87jUKWMf8Gpc64CdNymaXuchP0dHTvcZXtzHM_Tlw_nn6cdqdnnxafp-VtmaiaFqHJe85URwKYnhojOOGGG6klJrGstM20mjrW2ElY0lVpDWUScIdALKR_gZerfX3YxmDQsLYUi6V5vk1zrdqai9-rsS_Eot442ilDZCUlEU3twrpPhthDyotc8W-l4HiGNWnNSyqxnvdujrf9DrOKbymx1VvOjKMbRQbE_ZFHNO4A7bUKJ2jqq9o6rsr347qral6dXjOw4tDxYWgO-BXErFsfRn9n9kfwFCea5s</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3065093731</pqid></control><display><type>article</type><title>Mixed-phase weak anion-exchange/reversed-phase LC–MS/MS for analysis of nucleotide sugars in human fibroblasts</title><source>SpringerNature Journals</source><creator>Rahm, Moritz ; Kwast, Hanneke ; Wessels, Hans J. C. T. ; Noga, Marek J. ; Lefeber, Dirk J.</creator><creatorcontrib>Rahm, Moritz ; Kwast, Hanneke ; Wessels, Hans J. C. T. ; Noga, Marek J. ; Lefeber, Dirk J.</creatorcontrib><description>Nucleotide sugars (NS) fulfil important roles in all living organisms and in humans, related defects result in severe clinical syndromes. NS can be seen as the “activated” sugars used for biosynthesis of a wide range of glycoconjugates and serve as substrates themselves for the synthesis of other nucleotide sugars. NS analysis is complicated by the presence of multiple stereoisomers without diagnostic transition ions, therefore requiring separation by liquid chromatography. In this paper, we explored weak anion-exchange/reversed-phase chromatography on a hybrid column for the separation of 17 nucleotide sugars that can occur in humans. A robust and reproducible method was established with intra- and inter-day coefficients of variation below 10% and a linear range spanning three orders of magnitude. Application to patient fibroblasts with genetic defects in mannose-1-phosphate guanylyltransferase beta, CDP-
l
-ribitol pyrophosphorylase A, and UDP-
N
-acetylglucosamine 2-epimerase/
N
-acetylmannosamine kinase showed abnormal levels of guanosine-5′-diphosphate-α-
d
-mannose (GDP-Man), cytidine-5′-diphosphate-
l
-ribitol (CDP-ribitol), and cytidine-5′-monophosphate-
N
-acetyl-β-
d
-neuraminic acid (CMP-Neu5Ac), respectively, in consonance with expectations based on the diagnosis. In conclusion, a novel, semi-quantitative method was established for the analysis of nucleotide sugars that can be applied to diagnose several genetic glycosylation disorders in fibroblasts and beyond.</description><identifier>ISSN: 1618-2642</identifier><identifier>ISSN: 1618-2650</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-024-05313-w</identifier><identifier>PMID: 38676823</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Anion exchanging ; Biochemistry ; Biosynthesis ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Coefficient of variation ; Column chromatography ; Defects ; Epimerase ; Fibroblasts ; Food Science ; Glycoconjugates ; Glycosylation ; Kinases ; Laboratory Medicine ; Liquid chromatography ; Mannose ; Mannose-1-phosphate guanylyltransferase ; Monitoring/Environmental Analysis ; N-Acetylglucosamine ; Nucleotides ; Recent Advances in Ultrasensitive Omics Techniques ; Research Paper ; Separation ; Stereoisomerism ; Stereoisomers ; Substrates ; Sugar ; UDP-N-acetylglucosamine 2-epimerase</subject><ispartof>Analytical and bioanalytical chemistry, 2024-06, Vol.416 (15), p.3595-3604</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c426t-5f37383063770b369bf0b6b9b361cb5c2b897bacc56c75c0c608f1f60e96e0243</cites><orcidid>0000-0001-5957-3127 ; 0009-0008-8519-4794 ; 0000-0001-8352-611X ; 0000-0001-7770-8398</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-024-05313-w$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-024-05313-w$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38676823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahm, Moritz</creatorcontrib><creatorcontrib>Kwast, Hanneke</creatorcontrib><creatorcontrib>Wessels, Hans J. C. T.</creatorcontrib><creatorcontrib>Noga, Marek J.</creatorcontrib><creatorcontrib>Lefeber, Dirk J.</creatorcontrib><title>Mixed-phase weak anion-exchange/reversed-phase LC–MS/MS for analysis of nucleotide sugars in human fibroblasts</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Nucleotide sugars (NS) fulfil important roles in all living organisms and in humans, related defects result in severe clinical syndromes. NS can be seen as the “activated” sugars used for biosynthesis of a wide range of glycoconjugates and serve as substrates themselves for the synthesis of other nucleotide sugars. NS analysis is complicated by the presence of multiple stereoisomers without diagnostic transition ions, therefore requiring separation by liquid chromatography. In this paper, we explored weak anion-exchange/reversed-phase chromatography on a hybrid column for the separation of 17 nucleotide sugars that can occur in humans. A robust and reproducible method was established with intra- and inter-day coefficients of variation below 10% and a linear range spanning three orders of magnitude. Application to patient fibroblasts with genetic defects in mannose-1-phosphate guanylyltransferase beta, CDP-
l
-ribitol pyrophosphorylase A, and UDP-
N
-acetylglucosamine 2-epimerase/
N
-acetylmannosamine kinase showed abnormal levels of guanosine-5′-diphosphate-α-
d
-mannose (GDP-Man), cytidine-5′-diphosphate-
l
-ribitol (CDP-ribitol), and cytidine-5′-monophosphate-
N
-acetyl-β-
d
-neuraminic acid (CMP-Neu5Ac), respectively, in consonance with expectations based on the diagnosis. In conclusion, a novel, semi-quantitative method was established for the analysis of nucleotide sugars that can be applied to diagnose several genetic glycosylation disorders in fibroblasts and beyond.</description><subject>Analytical Chemistry</subject><subject>Anion exchanging</subject><subject>Biochemistry</subject><subject>Biosynthesis</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Coefficient of variation</subject><subject>Column chromatography</subject><subject>Defects</subject><subject>Epimerase</subject><subject>Fibroblasts</subject><subject>Food Science</subject><subject>Glycoconjugates</subject><subject>Glycosylation</subject><subject>Kinases</subject><subject>Laboratory Medicine</subject><subject>Liquid chromatography</subject><subject>Mannose</subject><subject>Mannose-1-phosphate guanylyltransferase</subject><subject>Monitoring/Environmental Analysis</subject><subject>N-Acetylglucosamine</subject><subject>Nucleotides</subject><subject>Recent Advances in Ultrasensitive Omics Techniques</subject><subject>Research Paper</subject><subject>Separation</subject><subject>Stereoisomerism</subject><subject>Stereoisomers</subject><subject>Substrates</subject><subject>Sugar</subject><subject>UDP-N-acetylglucosamine 2-epimerase</subject><issn>1618-2642</issn><issn>1618-2650</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kctuEzEUhi1ERS_wAiyQJTZshvgyY8-sEIpKQUrURWFt2c5x4jKxgz3TtDvegTfkSXBIGwoLVudI5zv_ufwIvaTkLSVETjIhjIqKsLoiDae82j5BJ1TQtmKiIU8Pec2O0WnO14TQpqXiGTrmrZCiZfwEbeb-FhbVZqUz4C3or1gHH0MFt3alwxImCW4g5QMym_78_mN-NZlfYRdTgXV_l33G0eEw2h7i4BeA87jUKWMf8Gpc64CdNymaXuchP0dHTvcZXtzHM_Tlw_nn6cdqdnnxafp-VtmaiaFqHJe85URwKYnhojOOGGG6klJrGstM20mjrW2ElY0lVpDWUScIdALKR_gZerfX3YxmDQsLYUi6V5vk1zrdqai9-rsS_Eot442ilDZCUlEU3twrpPhthDyotc8W-l4HiGNWnNSyqxnvdujrf9DrOKbymx1VvOjKMbRQbE_ZFHNO4A7bUKJ2jqq9o6rsr347qral6dXjOw4tDxYWgO-BXErFsfRn9n9kfwFCea5s</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Rahm, Moritz</creator><creator>Kwast, Hanneke</creator><creator>Wessels, Hans J. C. T.</creator><creator>Noga, Marek J.</creator><creator>Lefeber, Dirk J.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5957-3127</orcidid><orcidid>https://orcid.org/0009-0008-8519-4794</orcidid><orcidid>https://orcid.org/0000-0001-8352-611X</orcidid><orcidid>https://orcid.org/0000-0001-7770-8398</orcidid></search><sort><creationdate>20240601</creationdate><title>Mixed-phase weak anion-exchange/reversed-phase LC–MS/MS for analysis of nucleotide sugars in human fibroblasts</title><author>Rahm, Moritz ; Kwast, Hanneke ; Wessels, Hans J. C. T. ; Noga, Marek J. ; Lefeber, Dirk J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-5f37383063770b369bf0b6b9b361cb5c2b897bacc56c75c0c608f1f60e96e0243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analytical Chemistry</topic><topic>Anion exchanging</topic><topic>Biochemistry</topic><topic>Biosynthesis</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Coefficient of variation</topic><topic>Column chromatography</topic><topic>Defects</topic><topic>Epimerase</topic><topic>Fibroblasts</topic><topic>Food Science</topic><topic>Glycoconjugates</topic><topic>Glycosylation</topic><topic>Kinases</topic><topic>Laboratory Medicine</topic><topic>Liquid chromatography</topic><topic>Mannose</topic><topic>Mannose-1-phosphate guanylyltransferase</topic><topic>Monitoring/Environmental Analysis</topic><topic>N-Acetylglucosamine</topic><topic>Nucleotides</topic><topic>Recent Advances in Ultrasensitive Omics Techniques</topic><topic>Research Paper</topic><topic>Separation</topic><topic>Stereoisomerism</topic><topic>Stereoisomers</topic><topic>Substrates</topic><topic>Sugar</topic><topic>UDP-N-acetylglucosamine 2-epimerase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahm, Moritz</creatorcontrib><creatorcontrib>Kwast, Hanneke</creatorcontrib><creatorcontrib>Wessels, Hans J. C. T.</creatorcontrib><creatorcontrib>Noga, Marek J.</creatorcontrib><creatorcontrib>Lefeber, Dirk J.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahm, Moritz</au><au>Kwast, Hanneke</au><au>Wessels, Hans J. C. T.</au><au>Noga, Marek J.</au><au>Lefeber, Dirk J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mixed-phase weak anion-exchange/reversed-phase LC–MS/MS for analysis of nucleotide sugars in human fibroblasts</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>416</volume><issue>15</issue><spage>3595</spage><epage>3604</epage><pages>3595-3604</pages><issn>1618-2642</issn><issn>1618-2650</issn><eissn>1618-2650</eissn><abstract>Nucleotide sugars (NS) fulfil important roles in all living organisms and in humans, related defects result in severe clinical syndromes. NS can be seen as the “activated” sugars used for biosynthesis of a wide range of glycoconjugates and serve as substrates themselves for the synthesis of other nucleotide sugars. NS analysis is complicated by the presence of multiple stereoisomers without diagnostic transition ions, therefore requiring separation by liquid chromatography. In this paper, we explored weak anion-exchange/reversed-phase chromatography on a hybrid column for the separation of 17 nucleotide sugars that can occur in humans. A robust and reproducible method was established with intra- and inter-day coefficients of variation below 10% and a linear range spanning three orders of magnitude. Application to patient fibroblasts with genetic defects in mannose-1-phosphate guanylyltransferase beta, CDP-
l
-ribitol pyrophosphorylase A, and UDP-
N
-acetylglucosamine 2-epimerase/
N
-acetylmannosamine kinase showed abnormal levels of guanosine-5′-diphosphate-α-
d
-mannose (GDP-Man), cytidine-5′-diphosphate-
l
-ribitol (CDP-ribitol), and cytidine-5′-monophosphate-
N
-acetyl-β-
d
-neuraminic acid (CMP-Neu5Ac), respectively, in consonance with expectations based on the diagnosis. In conclusion, a novel, semi-quantitative method was established for the analysis of nucleotide sugars that can be applied to diagnose several genetic glycosylation disorders in fibroblasts and beyond.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38676823</pmid><doi>10.1007/s00216-024-05313-w</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5957-3127</orcidid><orcidid>https://orcid.org/0009-0008-8519-4794</orcidid><orcidid>https://orcid.org/0000-0001-8352-611X</orcidid><orcidid>https://orcid.org/0000-0001-7770-8398</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1618-2642 |
ispartof | Analytical and bioanalytical chemistry, 2024-06, Vol.416 (15), p.3595-3604 |
issn | 1618-2642 1618-2650 1618-2650 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11156716 |
source | SpringerNature Journals |
subjects | Analytical Chemistry Anion exchanging Biochemistry Biosynthesis Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatography Coefficient of variation Column chromatography Defects Epimerase Fibroblasts Food Science Glycoconjugates Glycosylation Kinases Laboratory Medicine Liquid chromatography Mannose Mannose-1-phosphate guanylyltransferase Monitoring/Environmental Analysis N-Acetylglucosamine Nucleotides Recent Advances in Ultrasensitive Omics Techniques Research Paper Separation Stereoisomerism Stereoisomers Substrates Sugar UDP-N-acetylglucosamine 2-epimerase |
title | Mixed-phase weak anion-exchange/reversed-phase LC–MS/MS for analysis of nucleotide sugars in human fibroblasts |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T10%3A42%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mixed-phase%20weak%20anion-exchange/reversed-phase%20LC%E2%80%93MS/MS%20for%20analysis%20of%20nucleotide%20sugars%20in%20human%20fibroblasts&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Rahm,%20Moritz&rft.date=2024-06-01&rft.volume=416&rft.issue=15&rft.spage=3595&rft.epage=3604&rft.pages=3595-3604&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-024-05313-w&rft_dat=%3Cproquest_pubme%3E3065093731%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3065093731&rft_id=info:pmid/38676823&rfr_iscdi=true |