Evidence for essential catalytic determinants for human erythrocyte pyrimidine 5'-nucleotidase

Human erythrocyte pyrimidine 5'-nucleotidase, PN-I, catalyzes the dephosphorylation of pyrimidine nucleoside monophosphates. The enzyme also possesses phosphotransferase activity, transferring phosphate groups between pyrimidine nucleoside monophosphates and various pyrimidine nucleosides. Defi...

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Veröffentlicht in:	Cellular and molecular life sciences : CMLS 2005-07, Vol.62 (14), p.1613-1620
Hauptverfasser:	Amici, A, Ciccioli, K, Naponelli, V, Raffaelli, N, Magni, G
Format:	Artikel
Sprache:	eng
Schlagworte:	5'-Nucleotidase - genetics 5'-Nucleotidase - isolation & purification 5'-Nucleotidase - metabolism Amino Acid Sequence Bioinformatics Catalysis Cloning, Molecular E coli Electrophoresis, Polyacrylamide Gel Enzymatic activity Erythrocytes Erythrocytes - enzymology Escherichia coli Humans Kinetics Mutagenesis, Site-Directed Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Alignment Sequence Homology, Amino Acid Substrate Specificity
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