Olfactory ensheathing cell phenotype following implantation in the lesioned spinal cord
Although olfactory ensheathing cells (OECs) are used to promote repair in the injured spinal cord, little is known of their phenotype in this environment. In this study, using quantitative reverse transcriptase-polymerase chain reaction RT-PCR, expression of neuregulin-1 mitogen/survival factors and...
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| Veröffentlicht in: | Cellular and molecular life sciences : CMLS 2003-10, Vol.60 (10), p.2241-2253 |
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| creator | Woodhall, E West, A K Vickers, J C Chuah, M I |
| description | Although olfactory ensheathing cells (OECs) are used to promote repair in the injured spinal cord, little is known of their phenotype in this environment. In this study, using quantitative reverse transcriptase-polymerase chain reaction RT-PCR, expression of neuregulin-1 mitogen/survival factors and the axonal growth regulator Nogo was quantified in OECs and compared with other non-neuronal cells. Their expression was also compared with OECs which had previously been encapsulated in a porous polymer tube and implanted into the injured spinal cord. Similar to astrocytes and fibroblasts, OECs expressed various neuregulin subtypes including neu differentiation factor, glial growth factor and sensory and motorneuron-derived factor. Implanted OECs upregulated neu differentiation factor and secreted neuregulin, but downregulated expression of all other variants. OECs and oligodendrocytes expressed Nogo-A, -B and -ABC and were immunopositive for Nogo-A protein. The Nogo-A protein in OECs was found to be cytoplasmic rather than nuclear or cell surface associated. Unlike oligodendrocytes, OECs expressed Nogo-66 receptor (NgR) mRNA. Implanted OECs upregulated Nogo-A and -B, but downregulated Nogo-ABC and NgR. |
| doi_str_mv | 10.1007/s00018-003-3265-7 |
| format | Article |
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In this study, using quantitative reverse transcriptase-polymerase chain reaction RT-PCR, expression of neuregulin-1 mitogen/survival factors and the axonal growth regulator Nogo was quantified in OECs and compared with other non-neuronal cells. Their expression was also compared with OECs which had previously been encapsulated in a porous polymer tube and implanted into the injured spinal cord. Similar to astrocytes and fibroblasts, OECs expressed various neuregulin subtypes including neu differentiation factor, glial growth factor and sensory and motorneuron-derived factor. Implanted OECs upregulated neu differentiation factor and secreted neuregulin, but downregulated expression of all other variants. OECs and oligodendrocytes expressed Nogo-A, -B and -ABC and were immunopositive for Nogo-A protein. The Nogo-A protein in OECs was found to be cytoplasmic rather than nuclear or cell surface associated. Unlike oligodendrocytes, OECs expressed Nogo-66 receptor (NgR) mRNA. Implanted OECs upregulated Nogo-A and -B, but downregulated Nogo-ABC and NgR.</description><identifier>ISSN: 1420-682X</identifier><identifier>EISSN: 1420-9071</identifier><identifier>DOI: 10.1007/s00018-003-3265-7</identifier><identifier>PMID: 14618270</identifier><language>eng</language><publisher>Switzerland: Springer Nature B.V</publisher><subject>Growth regulators ; Myelin Proteins - genetics ; Myelin Proteins - metabolism ; Neuregulin-1 - genetics ; Neuregulin-1 - metabolism ; Nogo Proteins ; Olfactory Mucosa - metabolism ; Olfactory Mucosa - transplantation ; Polymers ; Spinal Cord - metabolism ; Spinal cord injuries ; Spinal Cord Injuries - therapy ; Wound Healing - physiology</subject><ispartof>Cellular and molecular life sciences : CMLS, 2003-10, Vol.60 (10), p.2241-2253</ispartof><rights>Birkhäuser-Verlag Basel 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-df1574b559f131c11cf02c51ce8e9f8e4a58a66eb963a5bacb70959cb59acba13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11138761/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11138761/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14618270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woodhall, E</creatorcontrib><creatorcontrib>West, A K</creatorcontrib><creatorcontrib>Vickers, J C</creatorcontrib><creatorcontrib>Chuah, M I</creatorcontrib><title>Olfactory ensheathing cell phenotype following implantation in the lesioned spinal cord</title><title>Cellular and molecular life sciences : CMLS</title><addtitle>Cell Mol Life Sci</addtitle><description>Although olfactory ensheathing cells (OECs) are used to promote repair in the injured spinal cord, little is known of their phenotype in this environment. In this study, using quantitative reverse transcriptase-polymerase chain reaction RT-PCR, expression of neuregulin-1 mitogen/survival factors and the axonal growth regulator Nogo was quantified in OECs and compared with other non-neuronal cells. Their expression was also compared with OECs which had previously been encapsulated in a porous polymer tube and implanted into the injured spinal cord. Similar to astrocytes and fibroblasts, OECs expressed various neuregulin subtypes including neu differentiation factor, glial growth factor and sensory and motorneuron-derived factor. Implanted OECs upregulated neu differentiation factor and secreted neuregulin, but downregulated expression of all other variants. OECs and oligodendrocytes expressed Nogo-A, -B and -ABC and were immunopositive for Nogo-A protein. The Nogo-A protein in OECs was found to be cytoplasmic rather than nuclear or cell surface associated. Unlike oligodendrocytes, OECs expressed Nogo-66 receptor (NgR) mRNA. Implanted OECs upregulated Nogo-A and -B, but downregulated Nogo-ABC and NgR.</description><subject>Growth regulators</subject><subject>Myelin Proteins - genetics</subject><subject>Myelin Proteins - metabolism</subject><subject>Neuregulin-1 - genetics</subject><subject>Neuregulin-1 - metabolism</subject><subject>Nogo Proteins</subject><subject>Olfactory Mucosa - metabolism</subject><subject>Olfactory Mucosa - transplantation</subject><subject>Polymers</subject><subject>Spinal Cord - metabolism</subject><subject>Spinal cord injuries</subject><subject>Spinal Cord Injuries - therapy</subject><subject>Wound Healing - physiology</subject><issn>1420-682X</issn><issn>1420-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkktv1DAQxyMEoqXwAbggi0M5pXji-HVCqCoPqVIvILhZjnfSpPLawfaC9tvjaFe8DuXkseY3f83j3zTPgV4ApfJ1ppSCaillLesEb-WD5hT6jraaSnh4jIXqvp40T3K-qzBXnXjcnEAvQHWSnjZfbvxoXYlpTzDkCW2Z5nBLHHpPlglDLPsFyRi9jz_WxLxdvA3FljkGMgdSJiQec_3hhuRlDtYTF9PmafNotD7js-N71nx-d_Xp8kN7ffP-4-Xb69b1PSvtZgQu-4FzPQIDB-BG2jkODhXqUWFvubJC4KAFs3ywbpBUc-0GrmtsgZ01bw66y27Y4sZhKMl6s6R5a9PeRDubvzNhnsxt_G4AgCkpVoVXR4UUv-0wF7Od8zq_DRh32SjNOtlDJyt5fi8pgYme107_B4Ku1xKwKr78B7yLu1R3WMUE67vaoKoQHCCXYs4Jx1_TATWrD8zBB6b6wKw-MKvwiz_X8rvieHj2E0anr_k</recordid><startdate>200310</startdate><enddate>200310</enddate><creator>Woodhall, E</creator><creator>West, A K</creator><creator>Vickers, J C</creator><creator>Chuah, M I</creator><general>Springer Nature B.V</general><general>Birkhäuser-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7QO</scope><scope>5PM</scope></search><sort><creationdate>200310</creationdate><title>Olfactory ensheathing cell phenotype following implantation in the lesioned spinal cord</title><author>Woodhall, E ; West, A K ; Vickers, J C ; Chuah, M I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-df1574b559f131c11cf02c51ce8e9f8e4a58a66eb963a5bacb70959cb59acba13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Growth regulators</topic><topic>Myelin Proteins - genetics</topic><topic>Myelin Proteins - metabolism</topic><topic>Neuregulin-1 - genetics</topic><topic>Neuregulin-1 - metabolism</topic><topic>Nogo Proteins</topic><topic>Olfactory Mucosa - metabolism</topic><topic>Olfactory Mucosa - transplantation</topic><topic>Polymers</topic><topic>Spinal Cord - metabolism</topic><topic>Spinal cord injuries</topic><topic>Spinal Cord Injuries - therapy</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woodhall, E</creatorcontrib><creatorcontrib>West, A K</creatorcontrib><creatorcontrib>Vickers, J C</creatorcontrib><creatorcontrib>Chuah, M I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cellular and molecular life sciences : CMLS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woodhall, E</au><au>West, A K</au><au>Vickers, J C</au><au>Chuah, M I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Olfactory ensheathing cell phenotype following implantation in the lesioned spinal cord</atitle><jtitle>Cellular and molecular life sciences : CMLS</jtitle><addtitle>Cell Mol Life Sci</addtitle><date>2003-10</date><risdate>2003</risdate><volume>60</volume><issue>10</issue><spage>2241</spage><epage>2253</epage><pages>2241-2253</pages><issn>1420-682X</issn><eissn>1420-9071</eissn><abstract>Although olfactory ensheathing cells (OECs) are used to promote repair in the injured spinal cord, little is known of their phenotype in this environment. In this study, using quantitative reverse transcriptase-polymerase chain reaction RT-PCR, expression of neuregulin-1 mitogen/survival factors and the axonal growth regulator Nogo was quantified in OECs and compared with other non-neuronal cells. Their expression was also compared with OECs which had previously been encapsulated in a porous polymer tube and implanted into the injured spinal cord. Similar to astrocytes and fibroblasts, OECs expressed various neuregulin subtypes including neu differentiation factor, glial growth factor and sensory and motorneuron-derived factor. Implanted OECs upregulated neu differentiation factor and secreted neuregulin, but downregulated expression of all other variants. OECs and oligodendrocytes expressed Nogo-A, -B and -ABC and were immunopositive for Nogo-A protein. The Nogo-A protein in OECs was found to be cytoplasmic rather than nuclear or cell surface associated. Unlike oligodendrocytes, OECs expressed Nogo-66 receptor (NgR) mRNA. Implanted OECs upregulated Nogo-A and -B, but downregulated Nogo-ABC and NgR.</abstract><cop>Switzerland</cop><pub>Springer Nature B.V</pub><pmid>14618270</pmid><doi>10.1007/s00018-003-3265-7</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cellular and molecular life sciences : CMLS, 2003-10, Vol.60 (10), p.2241-2253 |
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| source | MEDLINE; SpringerNature Journals; PubMed Central |
| subjects | Growth regulators Myelin Proteins - genetics Myelin Proteins - metabolism Neuregulin-1 - genetics Neuregulin-1 - metabolism Nogo Proteins Olfactory Mucosa - metabolism Olfactory Mucosa - transplantation Polymers Spinal Cord - metabolism Spinal cord injuries Spinal Cord Injuries - therapy Wound Healing - physiology |
| title | Olfactory ensheathing cell phenotype following implantation in the lesioned spinal cord |
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