A morphological and immunohistochemical study of the effects of prednisolone or ursodeoxycholic acid on liver histology in feline lymphocytic cholangitis
Feline lymphocytic cholangitis (LC) has been commonly treated with prednisolone, and more recently with ursodeoxycholic acid (UDCA). Previously, we found that prednisolone treatment resulted in a statistically longer survival time than treatment with UDCA. In order to explain this difference, we com...
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| Veröffentlicht in: | Journal of feline medicine and surgery 2014-10, Vol.16 (10), p.796-804 |
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| creator | Otte, Corma MA Rothuizen, Jan Favier, Robert P Penning, Louis C Vreman, Sandra |
| description | Feline lymphocytic cholangitis (LC) has been commonly treated with prednisolone, and more recently with ursodeoxycholic acid (UDCA). Previously, we found that prednisolone treatment resulted in a statistically longer survival time than treatment with UDCA. In order to explain this difference, we compared the effects of prednisolone and UDCA treatment on hepatic tissue by evaluating consecutive liver biopsies. Archival serial biopsy materials from cats with LC treated with prednisolone (n = 5) or UDCA (n = 4) were evaluated. We employed haematoxylin and eosin staining to evaluate inflammation, and reticulin staining for fibrosis. Immunohistochemical stainings for Ki-67, K19 (Cytokeratin 19) and α-smooth muscle actin were used to evaluate cell type-specific proliferation and activation of hepatic stellate cells. Inflammation decreased more in the group treated with prednisolone, while the number of cholangiocytes, progenitor cells and fibroblasts did not differ between the treatment groups. Additionally, no difference was found for the amount of fibrosis in both treatment groups. |
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Previously, we found that prednisolone treatment resulted in a statistically longer survival time than treatment with UDCA. In order to explain this difference, we compared the effects of prednisolone and UDCA treatment on hepatic tissue by evaluating consecutive liver biopsies. Archival serial biopsy materials from cats with LC treated with prednisolone (n = 5) or UDCA (n = 4) were evaluated. We employed haematoxylin and eosin staining to evaluate inflammation, and reticulin staining for fibrosis. Immunohistochemical stainings for Ki-67, K19 (Cytokeratin 19) and α-smooth muscle actin were used to evaluate cell type-specific proliferation and activation of hepatic stellate cells. Inflammation decreased more in the group treated with prednisolone, while the number of cholangiocytes, progenitor cells and fibroblasts did not differ between the treatment groups. 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Previously, we found that prednisolone treatment resulted in a statistically longer survival time than treatment with UDCA. In order to explain this difference, we compared the effects of prednisolone and UDCA treatment on hepatic tissue by evaluating consecutive liver biopsies. Archival serial biopsy materials from cats with LC treated with prednisolone (n = 5) or UDCA (n = 4) were evaluated. We employed haematoxylin and eosin staining to evaluate inflammation, and reticulin staining for fibrosis. Immunohistochemical stainings for Ki-67, K19 (Cytokeratin 19) and α-smooth muscle actin were used to evaluate cell type-specific proliferation and activation of hepatic stellate cells. Inflammation decreased more in the group treated with prednisolone, while the number of cholangiocytes, progenitor cells and fibroblasts did not differ between the treatment groups. 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Rothuizen, Jan ; Favier, Robert P ; Penning, Louis C ; Vreman, Sandra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-7ecb09920702da1003a2a63efc5fd44d36acea6c83c421df4a96f7619c0883173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>actin</topic><topic>Animals</topic><topic>Biopsy</topic><topic>Cat Diseases - drug therapy</topic><topic>Cat Diseases - pathology</topic><topic>Cats</topic><topic>Cell Proliferation - drug effects</topic><topic>Cholangitis - drug therapy</topic><topic>Cholangitis - pathology</topic><topic>Cholangitis - veterinary</topic><topic>eosin</topic><topic>Female</topic><topic>fibroblasts</topic><topic>fibrosis</topic><topic>Immunohistochemistry</topic><topic>inflammation</topic><topic>liver</topic><topic>Liver - drug effects</topic><topic>Liver - pathology</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - pathology</topic><topic>Male</topic><topic>medicine</topic><topic>muscles</topic><topic>Original</topic><topic>prednisolone</topic><topic>Prednisolone - pharmacology</topic><topic>Prednisolone - therapeutic use</topic><topic>reticulin</topic><topic>ursodeoxycholic acid</topic><topic>Ursodeoxycholic Acid - pharmacology</topic><topic>Ursodeoxycholic Acid - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Otte, Corma MA</creatorcontrib><creatorcontrib>Rothuizen, Jan</creatorcontrib><creatorcontrib>Favier, Robert P</creatorcontrib><creatorcontrib>Penning, Louis C</creatorcontrib><creatorcontrib>Vreman, Sandra</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of feline medicine and surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Otte, Corma MA</au><au>Rothuizen, Jan</au><au>Favier, Robert P</au><au>Penning, Louis C</au><au>Vreman, Sandra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A morphological and immunohistochemical study of the effects of prednisolone or ursodeoxycholic acid on liver histology in feline lymphocytic cholangitis</atitle><jtitle>Journal of feline medicine and surgery</jtitle><addtitle>J Feline Med Surg</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>16</volume><issue>10</issue><spage>796</spage><epage>804</epage><pages>796-804</pages><issn>1098-612X</issn><issn>1532-2750</issn><eissn>1532-2750</eissn><abstract>Feline lymphocytic cholangitis (LC) has been commonly treated with prednisolone, and more recently with ursodeoxycholic acid (UDCA). Previously, we found that prednisolone treatment resulted in a statistically longer survival time than treatment with UDCA. In order to explain this difference, we compared the effects of prednisolone and UDCA treatment on hepatic tissue by evaluating consecutive liver biopsies. Archival serial biopsy materials from cats with LC treated with prednisolone (n = 5) or UDCA (n = 4) were evaluated. We employed haematoxylin and eosin staining to evaluate inflammation, and reticulin staining for fibrosis. Immunohistochemical stainings for Ki-67, K19 (Cytokeratin 19) and α-smooth muscle actin were used to evaluate cell type-specific proliferation and activation of hepatic stellate cells. Inflammation decreased more in the group treated with prednisolone, while the number of cholangiocytes, progenitor cells and fibroblasts did not differ between the treatment groups. 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| subjects | actin Animals Biopsy Cat Diseases - drug therapy Cat Diseases - pathology Cats Cell Proliferation - drug effects Cholangitis - drug therapy Cholangitis - pathology Cholangitis - veterinary eosin Female fibroblasts fibrosis Immunohistochemistry inflammation liver Liver - drug effects Liver - pathology Lymphocytes - drug effects Lymphocytes - pathology Male medicine muscles Original prednisolone Prednisolone - pharmacology Prednisolone - therapeutic use reticulin ursodeoxycholic acid Ursodeoxycholic Acid - pharmacology Ursodeoxycholic Acid - therapeutic use |
| title | A morphological and immunohistochemical study of the effects of prednisolone or ursodeoxycholic acid on liver histology in feline lymphocytic cholangitis |
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