Enhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene

Laccase, a copper-containing polyphenol oxidase, is an important green biocatalyst. In this study, Laccase Lcc5 was homologous recombinantly expressed in Coprinopsis cinerea and a novel strategy of silencing chitinase gene expression was used to enhance recombinant Lcc5 extracellular yield. Two crit...

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Veröffentlicht in:	Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.324-324, Article 324
Hauptverfasser:	Yao, Dongbang, Ma, Yuting, Ran, Jie, Wang, Jiaxiu, Kües, Ursula, Liu, Juanjuan, Zhou, Danya, Zhang, Xuecheng, Fang, Zemin, Xiao, Yazhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Agaricales - enzymology Agaricales - genetics Agitation biocatalysts Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology catechol oxidase Cell culture Cell Wall - genetics Cell Wall - metabolism Cell walls Chitinase Chitinases - biosynthesis Chitinases - genetics Chitinases - metabolism Coprinopsis cinerea Fermentation fermenters Fungal Proteins - genetics Fungal Proteins - metabolism Gene expression Gene Silencing Genes Genomes Homology Industrial production Laccase Laccase - biosynthesis Laccase - genetics Laccase - metabolism Life Sciences Microbial Genetics and Genomics Microbiology mycelium Mycelium - enzymology Mycelium - genetics Mycelium - growth & development Polyphenol oxidase Recombinant Proteins - genetics Recombinant Proteins - metabolism RNA Interference RNA-mediated interference Shear forces Sporulation transcriptome Transcriptomes
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