Two novel human and mouse DNA polymerases of the polX family

We describe here two novel mouse and human DNA polymerases: one (pol lambda) has homology with DNA polymerase beta while the other one (pol mu) is closer to terminal deoxynucleotidyltransferase. However both have DNA polymerase activity in vitro and share similar structural organization, including a...

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Veröffentlicht in:Nucleic acids research 2000-09, Vol.28 (18), p.3684-3693
Hauptverfasser: Aoufouchi, S, Flatter, E, Dahan, A, Faili, A, Bertocci, B, Storck, S, Delbos, F, Cocea, L, Gupta, N, Weill, J C, Reynaud, C A
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container_end_page 3693
container_issue 18
container_start_page 3684
container_title Nucleic acids research
container_volume 28
creator Aoufouchi, S
Flatter, E
Dahan, A
Faili, A
Bertocci, B
Storck, S
Delbos, F
Cocea, L
Gupta, N
Weill, J C
Reynaud, C A
description We describe here two novel mouse and human DNA polymerases: one (pol lambda) has homology with DNA polymerase beta while the other one (pol mu) is closer to terminal deoxynucleotidyltransferase. However both have DNA polymerase activity in vitro and share similar structural organization, including a BRCT domain, helix-loop-helix DNA-binding motifs and polymerase X domain. mRNA expression of pol lambda is highest in testis and fetal liver, while expression of pol mu is more lymphoid, with highest expression both in thymus and tonsillar B cells. An unusually large number of splice variants is observed for the pol mu gene, most of which affect the polymerase domain. Expression of mRNA of both polymerases is down-regulated upon treatment by DNA damaging agents (UV light, gamma-rays or H(2)O(2)). This suggests that their biological function may differ from DNA translesion synthesis, for which several DNA polymerase activities have been recently described. Possible functions are discussed.
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subjects Alternative Splicing
Amino Acid Sequence
Animals
Cell Line
Cloning, Molecular
DNA Damage
DNA Polymerase beta - chemistry
DNA Polymerase beta - classification
DNA, Complementary - isolation & purification
DNA-Directed DNA Polymerase - chemistry
DNA-Directed DNA Polymerase - classification
DNA-Directed DNA Polymerase - isolation & purification
Escherichia coli
Gene Expression Regulation, Enzymologic
Humans
Mice
Molecular Sequence Data
Phylogeny
Protein Conformation
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Tissue Distribution
Tumor Cells, Cultured
title Two novel human and mouse DNA polymerases of the polX family
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