Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast
Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the cent...
Gespeichert in:
| Veröffentlicht in: | Cellular and molecular life sciences : CMLS 2021-01, Vol.78 (1), p.373-384 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 384 |
|---|---|
| container_issue | 1 |
| container_start_page | 373 |
| container_title | Cellular and molecular life sciences : CMLS |
| container_volume | 78 |
| creator | Zhang, Min Zheng, Fan Xiong, Yujie Shao, Chen Wang, Chengliang Wu, Minhao Niu, Xiaojia Dong, Fenfen Zhang, Xuan Fu, Chuanhai Zang, Jianye |
| description | Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (
sp
Mis18,
sp
Mis16 and
sp
Mis19) is located to the centromere to license the recruitment of Cnp1
CENP-A
in
Schizosaccharomyces pombe
. We found that
sp
Mis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A–H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of the extreme C-terminus significantly impairs the localization of
sp
Mis18 and Cnp1 to the centromere and delays chromosome segregation during mitosis. Our findings illustrate that the interaction of
sp
Mis18 with histone H2A–H2B and DNA plays important roles in the recruitment of
sp
Mis18 and Cnp1 to the centromere in fission yeast. |
| doi_str_mv | 10.1007/s00018-020-03502-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11073290</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2486883592</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-f5b8429c54dcced60cb47673f6b42e0a238836a19ba3efc610bef748cb4c0e33</originalsourceid><addsrcrecordid>eNp9kbtOAzEQRS0E4hH4AQpkiXphbO_DW6EQHkHi0VBQYXmd2cSI7ILtgNLxD_whX4JDQoCGajyaM_eOdQnZZXDAAIpDDwBMJsAhAZEBT9gK2WRpbEso2OrinUt-t0G2vH-IdCZ5vk42BBdMFpncJPc9bIJrx-iQBu2GGGwzpG1Nr6xnkjp8nliHnoYRUtsEdNoE2zb01YYRPbnuUt0MaJ93P97e-_w4IrS23s-IKWoftslarR897ixqh9yend72-snlzflFr3uZmLTIQlJnlUx5abJ0YAwOcjBVWuSFqPMq5QiaCylFrllZaYG1yRlUWBepjJgBFKJDjuayT5NqjAMz-5N-VE_OjrWbqlZb9XfS2JEati-KMSgELyEq7C8UXPs8QR_UQztxTbxZ8VTm0T4reaT4nDKu9d5hvbRgoGaZqHkmKmaivjJRLC7t_T5uufIdQgTEHPBx1AzR_Xj_I_sJsKuYkw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2486883592</pqid></control><display><type>article</type><title>Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast</title><source>Open Access: PubMed Central</source><source>MEDLINE</source><source>SpringerLink (Online service)</source><creator>Zhang, Min ; Zheng, Fan ; Xiong, Yujie ; Shao, Chen ; Wang, Chengliang ; Wu, Minhao ; Niu, Xiaojia ; Dong, Fenfen ; Zhang, Xuan ; Fu, Chuanhai ; Zang, Jianye</creator><creatorcontrib>Zhang, Min ; Zheng, Fan ; Xiong, Yujie ; Shao, Chen ; Wang, Chengliang ; Wu, Minhao ; Niu, Xiaojia ; Dong, Fenfen ; Zhang, Xuan ; Fu, Chuanhai ; Zang, Jianye</creatorcontrib><description>Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (
sp
Mis18,
sp
Mis16 and
sp
Mis19) is located to the centromere to license the recruitment of Cnp1
CENP-A
in
Schizosaccharomyces pombe
. We found that
sp
Mis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A–H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of the extreme C-terminus significantly impairs the localization of
sp
Mis18 and Cnp1 to the centromere and delays chromosome segregation during mitosis. Our findings illustrate that the interaction of
sp
Mis18 with histone H2A–H2B and DNA plays important roles in the recruitment of
sp
Mis18 and Cnp1 to the centromere in fission yeast.</description><identifier>ISSN: 1420-682X</identifier><identifier>EISSN: 1420-9071</identifier><identifier>DOI: 10.1007/s00018-020-03502-1</identifier><identifier>PMID: 32318758</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Assembly ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; C-Terminus ; Carrier Proteins - chemistry ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cell Biology ; Centromere - metabolism ; Centromere protein A ; Chromatin ; Chromosomal Proteins, Non-Histone - genetics ; Chromosomal Proteins, Non-Histone - metabolism ; Chromosome Segregation ; Chromosomes ; Crystallography, X-Ray ; Deoxyribonucleic acid ; Dimerization ; Dimers ; DNA ; DNA - chemistry ; DNA - metabolism ; Fission ; Gene deletion ; Histone H2A ; Histone H3 ; Histones ; Histones - genetics ; Histones - metabolism ; Life Sciences ; Localization ; Microscopy, Fluorescence ; Mitosis ; Molecular Dynamics Simulation ; Multiprotein Complexes - chemistry ; Multiprotein Complexes - genetics ; Multiprotein Complexes - metabolism ; Mutagenesis ; Mutation ; Original ; Original Article ; Protein Binding ; Protein Domains ; Protein Structure, Tertiary ; Recruitment ; Schizosaccharomyces - genetics ; Schizosaccharomyces - metabolism ; Schizosaccharomyces pombe Proteins - chemistry ; Schizosaccharomyces pombe Proteins - genetics ; Schizosaccharomyces pombe Proteins - metabolism ; Time-Lapse Imaging ; Yeast ; Yeasts</subject><ispartof>Cellular and molecular life sciences : CMLS, 2021-01, Vol.78 (1), p.373-384</ispartof><rights>Springer Nature Switzerland AG 2020</rights><rights>Springer Nature Switzerland AG 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-f5b8429c54dcced60cb47673f6b42e0a238836a19ba3efc610bef748cb4c0e33</citedby><cites>FETCH-LOGICAL-c475t-f5b8429c54dcced60cb47673f6b42e0a238836a19ba3efc610bef748cb4c0e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11073290/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11073290/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32318758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Zheng, Fan</creatorcontrib><creatorcontrib>Xiong, Yujie</creatorcontrib><creatorcontrib>Shao, Chen</creatorcontrib><creatorcontrib>Wang, Chengliang</creatorcontrib><creatorcontrib>Wu, Minhao</creatorcontrib><creatorcontrib>Niu, Xiaojia</creatorcontrib><creatorcontrib>Dong, Fenfen</creatorcontrib><creatorcontrib>Zhang, Xuan</creatorcontrib><creatorcontrib>Fu, Chuanhai</creatorcontrib><creatorcontrib>Zang, Jianye</creatorcontrib><title>Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast</title><title>Cellular and molecular life sciences : CMLS</title><addtitle>Cell. Mol. Life Sci</addtitle><addtitle>Cell Mol Life Sci</addtitle><description>Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (
sp
Mis18,
sp
Mis16 and
sp
Mis19) is located to the centromere to license the recruitment of Cnp1
CENP-A
in
Schizosaccharomyces pombe
. We found that
sp
Mis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A–H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of the extreme C-terminus significantly impairs the localization of
sp
Mis18 and Cnp1 to the centromere and delays chromosome segregation during mitosis. Our findings illustrate that the interaction of
sp
Mis18 with histone H2A–H2B and DNA plays important roles in the recruitment of
sp
Mis18 and Cnp1 to the centromere in fission yeast.</description><subject>Assembly</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>C-Terminus</subject><subject>Carrier Proteins - chemistry</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Biology</subject><subject>Centromere - metabolism</subject><subject>Centromere protein A</subject><subject>Chromatin</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Chromosome Segregation</subject><subject>Chromosomes</subject><subject>Crystallography, X-Ray</subject><subject>Deoxyribonucleic acid</subject><subject>Dimerization</subject><subject>Dimers</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>Fission</subject><subject>Gene deletion</subject><subject>Histone H2A</subject><subject>Histone H3</subject><subject>Histones</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Microscopy, Fluorescence</subject><subject>Mitosis</subject><subject>Molecular Dynamics Simulation</subject><subject>Multiprotein Complexes - chemistry</subject><subject>Multiprotein Complexes - genetics</subject><subject>Multiprotein Complexes - metabolism</subject><subject>Mutagenesis</subject><subject>Mutation</subject><subject>Original</subject><subject>Original Article</subject><subject>Protein Binding</subject><subject>Protein Domains</subject><subject>Protein Structure, Tertiary</subject><subject>Recruitment</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - metabolism</subject><subject>Schizosaccharomyces pombe Proteins - chemistry</subject><subject>Schizosaccharomyces pombe Proteins - genetics</subject><subject>Schizosaccharomyces pombe Proteins - metabolism</subject><subject>Time-Lapse Imaging</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>1420-682X</issn><issn>1420-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kbtOAzEQRS0E4hH4AQpkiXphbO_DW6EQHkHi0VBQYXmd2cSI7ILtgNLxD_whX4JDQoCGajyaM_eOdQnZZXDAAIpDDwBMJsAhAZEBT9gK2WRpbEso2OrinUt-t0G2vH-IdCZ5vk42BBdMFpncJPc9bIJrx-iQBu2GGGwzpG1Nr6xnkjp8nliHnoYRUtsEdNoE2zb01YYRPbnuUt0MaJ93P97e-_w4IrS23s-IKWoftslarR897ixqh9yend72-snlzflFr3uZmLTIQlJnlUx5abJ0YAwOcjBVWuSFqPMq5QiaCylFrllZaYG1yRlUWBepjJgBFKJDjuayT5NqjAMz-5N-VE_OjrWbqlZb9XfS2JEati-KMSgELyEq7C8UXPs8QR_UQztxTbxZ8VTm0T4reaT4nDKu9d5hvbRgoGaZqHkmKmaivjJRLC7t_T5uufIdQgTEHPBx1AzR_Xj_I_sJsKuYkw</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Zhang, Min</creator><creator>Zheng, Fan</creator><creator>Xiong, Yujie</creator><creator>Shao, Chen</creator><creator>Wang, Chengliang</creator><creator>Wu, Minhao</creator><creator>Niu, Xiaojia</creator><creator>Dong, Fenfen</creator><creator>Zhang, Xuan</creator><creator>Fu, Chuanhai</creator><creator>Zang, Jianye</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20210101</creationdate><title>Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast</title><author>Zhang, Min ; Zheng, Fan ; Xiong, Yujie ; Shao, Chen ; Wang, Chengliang ; Wu, Minhao ; Niu, Xiaojia ; Dong, Fenfen ; Zhang, Xuan ; Fu, Chuanhai ; Zang, Jianye</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-f5b8429c54dcced60cb47673f6b42e0a238836a19ba3efc610bef748cb4c0e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Assembly</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>C-Terminus</topic><topic>Carrier Proteins - chemistry</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Biology</topic><topic>Centromere - metabolism</topic><topic>Centromere protein A</topic><topic>Chromatin</topic><topic>Chromosomal Proteins, Non-Histone - genetics</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>Chromosome Segregation</topic><topic>Chromosomes</topic><topic>Crystallography, X-Ray</topic><topic>Deoxyribonucleic acid</topic><topic>Dimerization</topic><topic>Dimers</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>DNA - metabolism</topic><topic>Fission</topic><topic>Gene deletion</topic><topic>Histone H2A</topic><topic>Histone H3</topic><topic>Histones</topic><topic>Histones - genetics</topic><topic>Histones - metabolism</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Microscopy, Fluorescence</topic><topic>Mitosis</topic><topic>Molecular Dynamics Simulation</topic><topic>Multiprotein Complexes - chemistry</topic><topic>Multiprotein Complexes - genetics</topic><topic>Multiprotein Complexes - metabolism</topic><topic>Mutagenesis</topic><topic>Mutation</topic><topic>Original</topic><topic>Original Article</topic><topic>Protein Binding</topic><topic>Protein Domains</topic><topic>Protein Structure, Tertiary</topic><topic>Recruitment</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - metabolism</topic><topic>Schizosaccharomyces pombe Proteins - chemistry</topic><topic>Schizosaccharomyces pombe Proteins - genetics</topic><topic>Schizosaccharomyces pombe Proteins - metabolism</topic><topic>Time-Lapse Imaging</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Zheng, Fan</creatorcontrib><creatorcontrib>Xiong, Yujie</creatorcontrib><creatorcontrib>Shao, Chen</creatorcontrib><creatorcontrib>Wang, Chengliang</creatorcontrib><creatorcontrib>Wu, Minhao</creatorcontrib><creatorcontrib>Niu, Xiaojia</creatorcontrib><creatorcontrib>Dong, Fenfen</creatorcontrib><creatorcontrib>Zhang, Xuan</creatorcontrib><creatorcontrib>Fu, Chuanhai</creatorcontrib><creatorcontrib>Zang, Jianye</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest_Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cellular and molecular life sciences : CMLS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Min</au><au>Zheng, Fan</au><au>Xiong, Yujie</au><au>Shao, Chen</au><au>Wang, Chengliang</au><au>Wu, Minhao</au><au>Niu, Xiaojia</au><au>Dong, Fenfen</au><au>Zhang, Xuan</au><au>Fu, Chuanhai</au><au>Zang, Jianye</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast</atitle><jtitle>Cellular and molecular life sciences : CMLS</jtitle><stitle>Cell. Mol. Life Sci</stitle><addtitle>Cell Mol Life Sci</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>78</volume><issue>1</issue><spage>373</spage><epage>384</epage><pages>373-384</pages><issn>1420-682X</issn><eissn>1420-9071</eissn><abstract>Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (
sp
Mis18,
sp
Mis16 and
sp
Mis19) is located to the centromere to license the recruitment of Cnp1
CENP-A
in
Schizosaccharomyces pombe
. We found that
sp
Mis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A–H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of the extreme C-terminus significantly impairs the localization of
sp
Mis18 and Cnp1 to the centromere and delays chromosome segregation during mitosis. Our findings illustrate that the interaction of
sp
Mis18 with histone H2A–H2B and DNA plays important roles in the recruitment of
sp
Mis18 and Cnp1 to the centromere in fission yeast.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32318758</pmid><doi>10.1007/s00018-020-03502-1</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1420-682X |
| ispartof | Cellular and molecular life sciences : CMLS, 2021-01, Vol.78 (1), p.373-384 |
| issn | 1420-682X 1420-9071 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11073290 |
| source | Open Access: PubMed Central; MEDLINE; SpringerLink (Online service) |
| subjects | Assembly Biochemistry Biomedical and Life Sciences Biomedicine C-Terminus Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - metabolism Cell Biology Centromere - metabolism Centromere protein A Chromatin Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism Chromosome Segregation Chromosomes Crystallography, X-Ray Deoxyribonucleic acid Dimerization Dimers DNA DNA - chemistry DNA - metabolism Fission Gene deletion Histone H2A Histone H3 Histones Histones - genetics Histones - metabolism Life Sciences Localization Microscopy, Fluorescence Mitosis Molecular Dynamics Simulation Multiprotein Complexes - chemistry Multiprotein Complexes - genetics Multiprotein Complexes - metabolism Mutagenesis Mutation Original Original Article Protein Binding Protein Domains Protein Structure, Tertiary Recruitment Schizosaccharomyces - genetics Schizosaccharomyces - metabolism Schizosaccharomyces pombe Proteins - chemistry Schizosaccharomyces pombe Proteins - genetics Schizosaccharomyces pombe Proteins - metabolism Time-Lapse Imaging Yeast Yeasts |
| title | Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T11%3A24%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Centromere%20targeting%20of%20Mis18%20requires%20the%20interaction%20with%20DNA%20and%20H2A%E2%80%93H2B%20in%20fission%20yeast&rft.jtitle=Cellular%20and%20molecular%20life%20sciences%20:%20CMLS&rft.au=Zhang,%20Min&rft.date=2021-01-01&rft.volume=78&rft.issue=1&rft.spage=373&rft.epage=384&rft.pages=373-384&rft.issn=1420-682X&rft.eissn=1420-9071&rft_id=info:doi/10.1007/s00018-020-03502-1&rft_dat=%3Cproquest_pubme%3E2486883592%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2486883592&rft_id=info:pmid/32318758&rfr_iscdi=true |