Quorum-sensing regulation of phenazine production heightens Pseudomonas aeruginosa resistance to ciprofloxacin
Quorum sensing is a type of cell-cell communication that modulates various biological activities of bacteria. Previous studies indicate that quorum sensing contributes to the evolution of bacterial resistance to antibiotics, but the underlying mechanisms are not fully understood. In this study, we g...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2024-05, Vol.68 (5), p.e0011824 |
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| container_title | Antimicrobial agents and chemotherapy |
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| creator | Xia, Lexin Li, Yue Wang, Yufan Zhou, Hui Dandekar, Ajai A Wang, Meizhen Xu, Feng |
| description | Quorum sensing is a type of cell-cell communication that modulates various biological activities of bacteria. Previous studies indicate that quorum sensing contributes to the evolution of bacterial resistance to antibiotics, but the underlying mechanisms are not fully understood. In this study, we grew
in the presence of sub-lethal concentrations of ciprofloxacin, resulting in a large increase in ciprofloxacin minimal inhibitory concentration. We discovered that quorum sensing-mediated phenazine biosynthesis was significantly enhanced in the resistant isolates, where the quinolone circuit was the predominant contributor to this phenomenon. We found that production of pyocyanin changed carbon flux and showed that the effect can be partially inhibited by the addition of pyruvate to cultures. This study illustrates the role of quorum sensing-mediated phenotypic resistance and suggests a strategy for its prevention. |
| doi_str_mv | 10.1128/aac.00118-24 |
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This study illustrates the role of quorum sensing-mediated phenotypic resistance and suggests a strategy for its prevention.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial Chemotherapy</subject><subject>Ciprofloxacin - pharmacology</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Gene Expression Regulation, Bacterial - drug effects</subject><subject>Mechanisms of Resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>Phenazines - metabolism</subject><subject>Phenazines - pharmacology</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pyocyanine - biosynthesis</subject><subject>Quinolones - pharmacology</subject><subject>Quorum Sensing - drug effects</subject><issn>0066-4804</issn><issn>1098-6596</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFPFTEQhxsikSd642z2KIkLbbfb154MISgkJEqi52a2nd1Xsts-260R_noXHhI5eJp0-vWbTH-EHDF6whhXpwD2hFLGVM3FHlkxqlUtWy1fkRWlUtZCUXFA3uR8S5dzq-lrctColksq1IqEmxJTmeqMIfswVAmHMsLsY6hiX203GODeB6y2KbpiH_sb9MNmXvjqW8bi4hQD5AowlcGHmGFxZJ9nCBarOVbWL2_7Mf4G68Nbst_DmPHdUz0kPz5ffD-_rK-_frk6P7uuoWnUXDPLnOoVW6-RCccdY32nUKrG8bXsbOOs1l3ToqYt1a6jPSiBICxXDDptVXNIPu2829JN6CyGOcFotslPkO5MBG9e3gS_MUP8ZRijUgjFFsOHJ0OKPwvm2Uw-WxxHCBhLNlyrdq2lEmJBP-5Qm2LOCfvnOYyah4zMkpF5zMjwB_x4h0OeuLmNJYXlK_7Hvv93j2fx3wCbP2zcnok</recordid><startdate>20240502</startdate><enddate>20240502</enddate><creator>Xia, Lexin</creator><creator>Li, Yue</creator><creator>Wang, Yufan</creator><creator>Zhou, Hui</creator><creator>Dandekar, Ajai A</creator><creator>Wang, Meizhen</creator><creator>Xu, Feng</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3169-0510</orcidid><orcidid>https://orcid.org/0000-0001-9867-8984</orcidid><orcidid>https://orcid.org/0000-0003-1034-8193</orcidid><orcidid>https://orcid.org/0009-0000-4574-508X</orcidid><orcidid>https://orcid.org/0009-0000-0799-6988</orcidid><orcidid>https://orcid.org/0000-0001-5764-6628</orcidid><orcidid>https://orcid.org/0000-0002-2992-6523</orcidid></search><sort><creationdate>20240502</creationdate><title>Quorum-sensing regulation of phenazine production heightens Pseudomonas aeruginosa resistance to ciprofloxacin</title><author>Xia, Lexin ; 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in the presence of sub-lethal concentrations of ciprofloxacin, resulting in a large increase in ciprofloxacin minimal inhibitory concentration. We discovered that quorum sensing-mediated phenazine biosynthesis was significantly enhanced in the resistant isolates, where the quinolone circuit was the predominant contributor to this phenomenon. We found that production of pyocyanin changed carbon flux and showed that the effect can be partially inhibited by the addition of pyruvate to cultures. This study illustrates the role of quorum sensing-mediated phenotypic resistance and suggests a strategy for its prevention.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>38526048</pmid><doi>10.1128/aac.00118-24</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-3169-0510</orcidid><orcidid>https://orcid.org/0000-0001-9867-8984</orcidid><orcidid>https://orcid.org/0000-0003-1034-8193</orcidid><orcidid>https://orcid.org/0009-0000-4574-508X</orcidid><orcidid>https://orcid.org/0009-0000-0799-6988</orcidid><orcidid>https://orcid.org/0000-0001-5764-6628</orcidid><orcidid>https://orcid.org/0000-0002-2992-6523</orcidid></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Antimicrobial Chemotherapy Ciprofloxacin - pharmacology Drug Resistance, Bacterial - genetics Gene Expression Regulation, Bacterial - drug effects Mechanisms of Resistance Microbial Sensitivity Tests Phenazines - metabolism Phenazines - pharmacology Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - genetics Pyocyanine - biosynthesis Quinolones - pharmacology Quorum Sensing - drug effects |
| title | Quorum-sensing regulation of phenazine production heightens Pseudomonas aeruginosa resistance to ciprofloxacin |
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