OptoChamber: A Low-cost, Easy-to-Make, Customizable, and Multi-Chambered Electronic Device for Applying Optogenetic Stimulation to Larval Drosophila melanogaster
Optogenetics is a powerful tool used to manipulate physiological processes in animals through cell-specific expression of genetically modified channelrhodopsins. In Drosophila melanogaster, optogenetics is frequently used for temporal control of neuronal activation or silencing through light-depende...
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| creator | Kann, Michael Ryan Ackerman, Martin K. Ackerman, Sarah D. |
| description | Optogenetics is a powerful tool used to manipulate physiological processes in animals through cell-specific expression of genetically modified channelrhodopsins. In Drosophila melanogaster, optogenetics is frequently used for temporal control of neuronal activation or silencing through light-dependent actuation of cation and anion channelrhodopsins, respectively. The high setup costs and complexity associated with commercially available optogenetic systems prevents many investigators from exploring the use of this technology. We developed a low-cost, customizable, and easy-to-make optogenetics chamber (OptoChamber) and verified its functionality in a robust cellular assay: activity-dependent remodeling of larval motor neurons in Drosophila embryos. |
| doi_str_mv | 10.17912/micropub.biology.001082 |
| format | Article |
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We developed a low-cost, customizable, and easy-to-make optogenetics chamber (OptoChamber) and verified its functionality in a robust cellular assay: activity-dependent remodeling of larval motor neurons in Drosophila embryos.</description><subject>Drosophila</subject><subject>Materials and Reagents</subject><subject>Methods</subject><subject>Phenotype Data</subject><subject>Successful</subject><issn>2578-9430</issn><issn>2578-9430</issn><fulltext>false</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PQ8</sourceid><recordid>eNpVUsFu2zAMNYYNa9H1FwYdd6gzybJla5chSLN1QIoeup0FWpYdbbLpSXKK7G_6p3WXtGhPJMHH90DyJQlhdMFKybLPvdUex6le1BYddvsFpYxW2ZvkNCvKKpU5p29f5CfJeQi_KaUZY2XJivfJCa9ExUSZnyb3N2PE1Rb62vgvZEk2eJdqDPGCrCHs04jpNfwxF2Q1hYi9_Qe1mysYGnI9uWjT46hpyNoZHT0OVpNLs7PakBY9WY6j29uhI486nRlMnPu30faTg2hxIBHJBvwOHLn0GHDcWgekNw4G7CBE4z8k71pwwZwf41ny69v65-oq3dx8_7FabtKGCZanbUtBgARazXUroJaFyKqK5XlDaybrTGqZQdM2Agqe1ZnQddEwaXjJtTZa8LPk64F3vmxvGm2G6MGp0dse_F4hWPW6M9it6nCnGKOFoCyfGT4dGTz-nUyIqrdBGzfvYnAKitO8yiWXnM_Qjy_FnlWeHjMDqgOggQjaRvMMYVT9t4F6soE62kAdbMAfAE-GrKM</recordid><startdate>2024</startdate><enddate>2024</enddate><creator>Kann, Michael Ryan</creator><creator>Ackerman, Martin K.</creator><creator>Ackerman, Sarah D.</creator><general>microPublication Biology</general><general>Caltech Library</general><scope>PQ8</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2024</creationdate><title>OptoChamber: A Low-cost, Easy-to-Make, Customizable, and Multi-Chambered Electronic Device for Applying Optogenetic Stimulation to Larval Drosophila melanogaster</title><author>Kann, Michael Ryan ; Ackerman, Martin K. ; Ackerman, Sarah D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d1614-ff0a6a9a08d16f6ab956288144d0b19b29c92adfd6a532b26cb5d19e373ccec63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Drosophila</topic><topic>Materials and Reagents</topic><topic>Methods</topic><topic>Phenotype Data</topic><topic>Successful</topic><toplevel>peer_reviewed</toplevel><creatorcontrib>Kann, Michael Ryan</creatorcontrib><creatorcontrib>Ackerman, Martin K.</creatorcontrib><creatorcontrib>Ackerman, Sarah D.</creatorcontrib><collection>DataCite</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>microPublication biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>no_fulltext_linktorsrc</fulltext></delivery><addata><au>Kann, Michael Ryan</au><au>Ackerman, Martin K.</au><au>Ackerman, Sarah D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>OptoChamber: A Low-cost, Easy-to-Make, Customizable, and Multi-Chambered Electronic Device for Applying Optogenetic Stimulation to Larval Drosophila melanogaster</atitle><jtitle>microPublication biology</jtitle><addtitle>MicroPubl Biol</addtitle><date>2024</date><risdate>2024</risdate><volume>2024</volume><issn>2578-9430</issn><eissn>2578-9430</eissn><abstract>Optogenetics is a powerful tool used to manipulate physiological processes in animals through cell-specific expression of genetically modified channelrhodopsins. 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| subjects | Drosophila Materials and Reagents Methods Phenotype Data Successful |
| title | OptoChamber: A Low-cost, Easy-to-Make, Customizable, and Multi-Chambered Electronic Device for Applying Optogenetic Stimulation to Larval Drosophila melanogaster |
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