Immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane
Purpose The main purpose of this study was to perform an immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane (ERM). Methods Twenty-four specimens of idiopathic ERM from 24 consecutive patients who underwent 23 G pars plana vitrectomy for ERM and...
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| creator | Martín, Julio Cesar Molina Sánchez, Laura Fernández Piñero, David P. Navarro, Nicolás Cuenca |
| description | Purpose
The main purpose of this study was to perform an immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane (ERM).
Methods
Twenty-four specimens of idiopathic ERM from 24 consecutive patients who underwent 23 G pars plana vitrectomy for ERM and internal limiting membrane (ILM) peeling at the San Juan University Hospital in Alicante (Spain) in 2019 were analyzed. All patients underwent a complete ophthalmological examination including measurement of best corrected visual acuity (BCVA) and macular analysis by spectral-domain optical coherence tomography (SD-OCT) at the time of diagnosis and 3 months after surgery. Specific glial fibrillar acid protein antibodies (GFAP) and S100 calcium-binding protein β (S100β) immunostaining markers were used to identify the macroglial component of the ERM, Müller cells, and astrocytes. Ionized calcium-binding adapter molecule 1 protein (Iba1) antibodies were used as specific markers for inflammatory cells, such as microglia and macrophages.
Results
Mean preoperative BCVA measured with Snellen chart was 0.3 and 0.6 preoperatively and at 3 months after surgery, respectively. SD-OCT identified 15 patients (62.5%) with a disruption of the outer retinal hyperreflective bands. The immunohistochemical study showed the presence of Müller cells in almost all cases (91.6%), as well of abundant microglia and macrophages. Microglia and macrophages were more frequently present in earlier stages of ERM. Microglia were present in ERM independently of the outer retinal hyperreflective bands integrity as measured by SD-OCT. A greater presence of macrophages was found in those ERMs with no outer retinal hyperreflective band disruption.
Conclusions
Müller cells seem to be the most frequent cell group in ERMs, with also presence of microglia cells and macrophages. Astrocytes were more frequently found in early stages of ERMs. Microglia and macrophages were most frequent in ERMs with early stage (1, 2, or 3) than in advanced stages (4). |
| doi_str_mv | 10.1007/s00417-023-06366-w |
| format | Article |
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The main purpose of this study was to perform an immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane (ERM).
Methods
Twenty-four specimens of idiopathic ERM from 24 consecutive patients who underwent 23 G pars plana vitrectomy for ERM and internal limiting membrane (ILM) peeling at the San Juan University Hospital in Alicante (Spain) in 2019 were analyzed. All patients underwent a complete ophthalmological examination including measurement of best corrected visual acuity (BCVA) and macular analysis by spectral-domain optical coherence tomography (SD-OCT) at the time of diagnosis and 3 months after surgery. Specific glial fibrillar acid protein antibodies (GFAP) and S100 calcium-binding protein β (S100β) immunostaining markers were used to identify the macroglial component of the ERM, Müller cells, and astrocytes. Ionized calcium-binding adapter molecule 1 protein (Iba1) antibodies were used as specific markers for inflammatory cells, such as microglia and macrophages.
Results
Mean preoperative BCVA measured with Snellen chart was 0.3 and 0.6 preoperatively and at 3 months after surgery, respectively. SD-OCT identified 15 patients (62.5%) with a disruption of the outer retinal hyperreflective bands. The immunohistochemical study showed the presence of Müller cells in almost all cases (91.6%), as well of abundant microglia and macrophages. Microglia and macrophages were more frequently present in earlier stages of ERM. Microglia were present in ERM independently of the outer retinal hyperreflective bands integrity as measured by SD-OCT. A greater presence of macrophages was found in those ERMs with no outer retinal hyperreflective band disruption.
Conclusions
Müller cells seem to be the most frequent cell group in ERMs, with also presence of microglia cells and macrophages. Astrocytes were more frequently found in early stages of ERMs. Microglia and macrophages were most frequent in ERMs with early stage (1, 2, or 3) than in advanced stages (4).</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-023-06366-w</identifier><identifier>PMID: 38197992</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acuity ; Antibodies ; Astrocytes ; Calcium-binding protein ; Glial fibrillary acidic protein ; Immunohistochemistry ; Inflammation ; Macrophages ; Medicine ; Medicine & Public Health ; Microglia ; Mueller cells ; Ophthalmology ; Patients ; Proteins ; Retina ; Retinal Disorders ; Surgery</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2024-05, Vol.262 (5), p.1443-1453</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c426t-d74b8ec4a811dbc08e03147da6a982d8b7fda1e7a67f93020a2823b9f50451963</cites><orcidid>0000-0002-1546-4807</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00417-023-06366-w$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00417-023-06366-w$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,27926,27927,41490,42559,51321</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38197992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martín, Julio Cesar Molina</creatorcontrib><creatorcontrib>Sánchez, Laura Fernández</creatorcontrib><creatorcontrib>Piñero, David P.</creatorcontrib><creatorcontrib>Navarro, Nicolás Cuenca</creatorcontrib><title>Immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Purpose
The main purpose of this study was to perform an immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane (ERM).
Methods
Twenty-four specimens of idiopathic ERM from 24 consecutive patients who underwent 23 G pars plana vitrectomy for ERM and internal limiting membrane (ILM) peeling at the San Juan University Hospital in Alicante (Spain) in 2019 were analyzed. All patients underwent a complete ophthalmological examination including measurement of best corrected visual acuity (BCVA) and macular analysis by spectral-domain optical coherence tomography (SD-OCT) at the time of diagnosis and 3 months after surgery. Specific glial fibrillar acid protein antibodies (GFAP) and S100 calcium-binding protein β (S100β) immunostaining markers were used to identify the macroglial component of the ERM, Müller cells, and astrocytes. Ionized calcium-binding adapter molecule 1 protein (Iba1) antibodies were used as specific markers for inflammatory cells, such as microglia and macrophages.
Results
Mean preoperative BCVA measured with Snellen chart was 0.3 and 0.6 preoperatively and at 3 months after surgery, respectively. SD-OCT identified 15 patients (62.5%) with a disruption of the outer retinal hyperreflective bands. The immunohistochemical study showed the presence of Müller cells in almost all cases (91.6%), as well of abundant microglia and macrophages. Microglia and macrophages were more frequently present in earlier stages of ERM. Microglia were present in ERM independently of the outer retinal hyperreflective bands integrity as measured by SD-OCT. A greater presence of macrophages was found in those ERMs with no outer retinal hyperreflective band disruption.
Conclusions
Müller cells seem to be the most frequent cell group in ERMs, with also presence of microglia cells and macrophages. Astrocytes were more frequently found in early stages of ERMs. Microglia and macrophages were most frequent in ERMs with early stage (1, 2, or 3) than in advanced stages (4).</description><subject>Acuity</subject><subject>Antibodies</subject><subject>Astrocytes</subject><subject>Calcium-binding protein</subject><subject>Glial fibrillary acidic protein</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Macrophages</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microglia</subject><subject>Mueller cells</subject><subject>Ophthalmology</subject><subject>Patients</subject><subject>Proteins</subject><subject>Retina</subject><subject>Retinal Disorders</subject><subject>Surgery</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kU1P3DAQhq2qqLvQ_oEeqkhcOBAY20nsnFC14ktC6qVI3CzHcTZeJfHWTlj13zO7CxQ49GB55HnmnRm_hHyncEYBxHkEyKhIgfEUCl4U6eYTmdOM56kA9vCZzEEwmkrOHmbkMMYVIM9z-oXMuKSlKEs2J6vbvp8G37o4etPa3hndnSbNNJjR-WEb66HGo0e_yyX2UXeT3iYT3yRrjOwwxmTjxjZxtfP40jqT2LULdnSokPS2r4Ie7Fdy0Ogu2m_P9xG5v7r8vbhJ735d3y5-3qUmY8WY1iKrpDWZlpTWlQFpgdNM1LrQpWS1rERTa2qFLkRTcmCgmWS8KpscspyWBT8iF3vd9VT1tjY4X9CdWgfX6_BXee3U-8zgWrX0j4rSbaeSosLJs0LwfyYbR9W7aGzX4RZ-ioohAxJkliN6_AFd-Sng2lFxdKfA32eAFNtTJvgYg21ep6Ggtl6qvZcKvVQ7L9UGi3683eO15MU8BPgeiJgaljb86_0f2SeCuq09</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Martín, Julio Cesar Molina</creator><creator>Sánchez, Laura Fernández</creator><creator>Piñero, David P.</creator><creator>Navarro, Nicolás Cuenca</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1546-4807</orcidid></search><sort><creationdate>20240501</creationdate><title>Immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane</title><author>Martín, Julio Cesar Molina ; Sánchez, Laura Fernández ; Piñero, David P. ; Navarro, Nicolás Cuenca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-d74b8ec4a811dbc08e03147da6a982d8b7fda1e7a67f93020a2823b9f50451963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acuity</topic><topic>Antibodies</topic><topic>Astrocytes</topic><topic>Calcium-binding protein</topic><topic>Glial fibrillary acidic protein</topic><topic>Immunohistochemistry</topic><topic>Inflammation</topic><topic>Macrophages</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microglia</topic><topic>Mueller cells</topic><topic>Ophthalmology</topic><topic>Patients</topic><topic>Proteins</topic><topic>Retina</topic><topic>Retinal Disorders</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martín, Julio Cesar Molina</creatorcontrib><creatorcontrib>Sánchez, Laura Fernández</creatorcontrib><creatorcontrib>Piñero, David P.</creatorcontrib><creatorcontrib>Navarro, Nicolás Cuenca</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martín, Julio Cesar Molina</au><au>Sánchez, Laura Fernández</au><au>Piñero, David P.</au><au>Navarro, Nicolás Cuenca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><stitle>Graefes Arch Clin Exp Ophthalmol</stitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2024-05-01</date><risdate>2024</risdate><volume>262</volume><issue>5</issue><spage>1443</spage><epage>1453</epage><pages>1443-1453</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><abstract>Purpose
The main purpose of this study was to perform an immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane (ERM).
Methods
Twenty-four specimens of idiopathic ERM from 24 consecutive patients who underwent 23 G pars plana vitrectomy for ERM and internal limiting membrane (ILM) peeling at the San Juan University Hospital in Alicante (Spain) in 2019 were analyzed. All patients underwent a complete ophthalmological examination including measurement of best corrected visual acuity (BCVA) and macular analysis by spectral-domain optical coherence tomography (SD-OCT) at the time of diagnosis and 3 months after surgery. Specific glial fibrillar acid protein antibodies (GFAP) and S100 calcium-binding protein β (S100β) immunostaining markers were used to identify the macroglial component of the ERM, Müller cells, and astrocytes. Ionized calcium-binding adapter molecule 1 protein (Iba1) antibodies were used as specific markers for inflammatory cells, such as microglia and macrophages.
Results
Mean preoperative BCVA measured with Snellen chart was 0.3 and 0.6 preoperatively and at 3 months after surgery, respectively. SD-OCT identified 15 patients (62.5%) with a disruption of the outer retinal hyperreflective bands. The immunohistochemical study showed the presence of Müller cells in almost all cases (91.6%), as well of abundant microglia and macrophages. Microglia and macrophages were more frequently present in earlier stages of ERM. Microglia were present in ERM independently of the outer retinal hyperreflective bands integrity as measured by SD-OCT. A greater presence of macrophages was found in those ERMs with no outer retinal hyperreflective band disruption.
Conclusions
Müller cells seem to be the most frequent cell group in ERMs, with also presence of microglia cells and macrophages. Astrocytes were more frequently found in early stages of ERMs. Microglia and macrophages were most frequent in ERMs with early stage (1, 2, or 3) than in advanced stages (4).</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38197992</pmid><doi>10.1007/s00417-023-06366-w</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1546-4807</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acuity Antibodies Astrocytes Calcium-binding protein Glial fibrillary acidic protein Immunohistochemistry Inflammation Macrophages Medicine Medicine & Public Health Microglia Mueller cells Ophthalmology Patients Proteins Retina Retinal Disorders Surgery |
| title | Immunohistochemical, functional, and anatomical evaluation of patients with idiopathic epiretinal membrane |
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