Human tankyrases are aberrantly expressed in colon tumors and contain multiple epitopes that induce humoral and cellular immune responses in cancer patients
Purpose Tankyrases 1 and 2 are telomere-associated poly(ADP-ribose) polymerases (PARP) that can positively regulate telomere elongation and interact with multiple cellular proteins. Recent reports implicated tankyrases as tumor antigens and potential targets of anticancer treatment. We examined expr...
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| Veröffentlicht in: | Cancer Immunology, Immunotherapy Immunotherapy, 2008-06, Vol.57 (6), p.871-881 |
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| creator | Shebzukhov, Yuriy V. Lavrik, Inna N. Karbach, Julia Khlgatian, Svetlana V. Koroleva, Ekaterina P. Belousov, Pavel V. Kashkin, Kirill N. Knuth, Alexander Jager, Elke Chi, Nai-Wen Kuprash, Dmitry V. Nedospasov, Sergei A. |
| description | Purpose
Tankyrases 1 and 2 are telomere-associated poly(ADP-ribose) polymerases (PARP) that can positively regulate telomere elongation and interact with multiple cellular proteins. Recent reports implicated tankyrases as tumor antigens and potential targets of anticancer treatment. We examined expression of tankyrases in colon tumors and immune response to these enzymes in patients with different types of cancer.
Methods
mRNA and protein expression was evaluated by quantitative real-time RT-PCR and Western blotting, respectively. Humoral immune response to recombinant tankyrases was investigated by modified enzyme-linked immunoassays. Cellular immune response was analysed by ELISPOT and
51
Cr release assays.
Results
We found that both mRNA and protein levels of tankyrase 2 (TNKL) are upregulated in colon tumors. In contrast, protein level of tankyrase 1 (TNKS) is downregulated, while mRNA level shows variable changes. More than a quarter of colon cancer patients develop humoral immune response to at least one of the two tankyrases. In this study we mapped common and unique B-cell epitopes located in different domains of the two proteins. Additionally, we present evidence for T-cell responses both to epitopes that are unique for TNKL and to those shared between TNKL and TNKS.
Conclusion
Our study favors a biomarker usage of antibody response to tankyrases. Spontaneous CD8
+
T-cell responses to these enzymes are rare and further investigation is needed to evaluate tankyrases as potential targets for cancer immunotherapy. |
| doi_str_mv | 10.1007/s00262-007-0423-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11030928</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1457589911</sourcerecordid><originalsourceid>FETCH-LOGICAL-c517t-fadf2d323a2c4c346972bed1549e524ba19bc53f14d72d210b6ce137761678dc3</originalsourceid><addsrcrecordid>eNqNks1u1TAQhSMEopfCA7BBFhLsAv5LnKwQqoAiVWIDa8txJr0pjh1sp-L2WXhYJspVC0gIVh55vnPGP6conjL6ilGqXidKec1LLEsquShv7hU7JgXuNBW7X-yokLRUlMqT4lFKV1hw2rYPixPWoLCt2K74cb5MxpNs_NdDNAkSMRGI6SBG47M7EPg-R0gJejJ6YoMLCC9TiAj6Hjd8NtiYFpfH2QGBecxhRpu8Nxkl_WKB7FeBcZsCnFuciWScpsUDQfM5-HXw6m-8hUhmk0fwOT0uHgzGJXhyXE-LL-_ffT47Ly8-ffh49vaitBVTuRxMP_BecGG4lVbIulW8g55VsoWKy86wtrOVGJjsFe85o11tgQmlalarprfitHiz-c5LN0FvcTYeV89xnEw86GBG_XvHj3t9Ga41Y1TQljfo8PLoEMO3BVLW05jWqxoPYUlaUYl_0db_BDlVjWhq9R9grSpereDzP8CrsESP76U5ExUTtFrHsg2yMaQUYbi9HKN6zZLesqTXcs2SvkHNs19f5U5xDA8CL46ASda4ARNjx3TLccqVZC1Hjm9cwpa_hHh3wr9P_wlPRuYD</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213513056</pqid></control><display><type>article</type><title>Human tankyrases are aberrantly expressed in colon tumors and contain multiple epitopes that induce humoral and cellular immune responses in cancer patients</title><source>MEDLINE</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Shebzukhov, Yuriy V. ; Lavrik, Inna N. ; Karbach, Julia ; Khlgatian, Svetlana V. ; Koroleva, Ekaterina P. ; Belousov, Pavel V. ; Kashkin, Kirill N. ; Knuth, Alexander ; Jager, Elke ; Chi, Nai-Wen ; Kuprash, Dmitry V. ; Nedospasov, Sergei A.</creator><creatorcontrib>Shebzukhov, Yuriy V. ; Lavrik, Inna N. ; Karbach, Julia ; Khlgatian, Svetlana V. ; Koroleva, Ekaterina P. ; Belousov, Pavel V. ; Kashkin, Kirill N. ; Knuth, Alexander ; Jager, Elke ; Chi, Nai-Wen ; Kuprash, Dmitry V. ; Nedospasov, Sergei A.</creatorcontrib><description>Purpose
Tankyrases 1 and 2 are telomere-associated poly(ADP-ribose) polymerases (PARP) that can positively regulate telomere elongation and interact with multiple cellular proteins. Recent reports implicated tankyrases as tumor antigens and potential targets of anticancer treatment. We examined expression of tankyrases in colon tumors and immune response to these enzymes in patients with different types of cancer.
Methods
mRNA and protein expression was evaluated by quantitative real-time RT-PCR and Western blotting, respectively. Humoral immune response to recombinant tankyrases was investigated by modified enzyme-linked immunoassays. Cellular immune response was analysed by ELISPOT and
51
Cr release assays.
Results
We found that both mRNA and protein levels of tankyrase 2 (TNKL) are upregulated in colon tumors. In contrast, protein level of tankyrase 1 (TNKS) is downregulated, while mRNA level shows variable changes. More than a quarter of colon cancer patients develop humoral immune response to at least one of the two tankyrases. In this study we mapped common and unique B-cell epitopes located in different domains of the two proteins. Additionally, we present evidence for T-cell responses both to epitopes that are unique for TNKL and to those shared between TNKL and TNKS.
Conclusion
Our study favors a biomarker usage of antibody response to tankyrases. Spontaneous CD8
+
T-cell responses to these enzymes are rare and further investigation is needed to evaluate tankyrases as potential targets for cancer immunotherapy.</description><identifier>ISSN: 0340-7004</identifier><identifier>EISSN: 1432-0851</identifier><identifier>DOI: 10.1007/s00262-007-0423-z</identifier><identifier>PMID: 18026951</identifier><identifier>CODEN: CIIMDN</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Amino Acid Sequence ; Antibody Formation ; Antigens ; Biomarkers, Tumor - metabolism ; Cancer ; Cancer Research ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - metabolism ; Cell division ; Colon ; Colonic Neoplasms - enzymology ; Colonic Neoplasms - pathology ; Enzymes ; Epitope Mapping - methods ; Epitopes - chemistry ; Humans ; Immunity, Cellular ; Immunology ; Immunotherapy - methods ; Investigations ; Leukemia ; Medicine ; Medicine & Public Health ; Molecular Sequence Data ; Neoplasms - immunology ; Neoplasms - therapy ; Oncology ; Original ; Original Article ; Proteins ; Sequence Homology, Amino Acid ; Serology ; Tankyrases - biosynthesis ; Tankyrases - metabolism ; Telomerase ; Tumors</subject><ispartof>Cancer Immunology, Immunotherapy, 2008-06, Vol.57 (6), p.871-881</ispartof><rights>Springer-Verlag 2007</rights><rights>2008 INIST-CNRS</rights><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-fadf2d323a2c4c346972bed1549e524ba19bc53f14d72d210b6ce137761678dc3</citedby><cites>FETCH-LOGICAL-c517t-fadf2d323a2c4c346972bed1549e524ba19bc53f14d72d210b6ce137761678dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11030928/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11030928/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20274192$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18026951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shebzukhov, Yuriy V.</creatorcontrib><creatorcontrib>Lavrik, Inna N.</creatorcontrib><creatorcontrib>Karbach, Julia</creatorcontrib><creatorcontrib>Khlgatian, Svetlana V.</creatorcontrib><creatorcontrib>Koroleva, Ekaterina P.</creatorcontrib><creatorcontrib>Belousov, Pavel V.</creatorcontrib><creatorcontrib>Kashkin, Kirill N.</creatorcontrib><creatorcontrib>Knuth, Alexander</creatorcontrib><creatorcontrib>Jager, Elke</creatorcontrib><creatorcontrib>Chi, Nai-Wen</creatorcontrib><creatorcontrib>Kuprash, Dmitry V.</creatorcontrib><creatorcontrib>Nedospasov, Sergei A.</creatorcontrib><title>Human tankyrases are aberrantly expressed in colon tumors and contain multiple epitopes that induce humoral and cellular immune responses in cancer patients</title><title>Cancer Immunology, Immunotherapy</title><addtitle>Cancer Immunol Immunother</addtitle><addtitle>Cancer Immunol Immunother</addtitle><description>Purpose
Tankyrases 1 and 2 are telomere-associated poly(ADP-ribose) polymerases (PARP) that can positively regulate telomere elongation and interact with multiple cellular proteins. Recent reports implicated tankyrases as tumor antigens and potential targets of anticancer treatment. We examined expression of tankyrases in colon tumors and immune response to these enzymes in patients with different types of cancer.
Methods
mRNA and protein expression was evaluated by quantitative real-time RT-PCR and Western blotting, respectively. Humoral immune response to recombinant tankyrases was investigated by modified enzyme-linked immunoassays. Cellular immune response was analysed by ELISPOT and
51
Cr release assays.
Results
We found that both mRNA and protein levels of tankyrase 2 (TNKL) are upregulated in colon tumors. In contrast, protein level of tankyrase 1 (TNKS) is downregulated, while mRNA level shows variable changes. More than a quarter of colon cancer patients develop humoral immune response to at least one of the two tankyrases. In this study we mapped common and unique B-cell epitopes located in different domains of the two proteins. Additionally, we present evidence for T-cell responses both to epitopes that are unique for TNKL and to those shared between TNKL and TNKS.
Conclusion
Our study favors a biomarker usage of antibody response to tankyrases. Spontaneous CD8
+
T-cell responses to these enzymes are rare and further investigation is needed to evaluate tankyrases as potential targets for cancer immunotherapy.</description><subject>Amino Acid Sequence</subject><subject>Antibody Formation</subject><subject>Antigens</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Cancer</subject><subject>Cancer Research</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cell division</subject><subject>Colon</subject><subject>Colonic Neoplasms - enzymology</subject><subject>Colonic Neoplasms - pathology</subject><subject>Enzymes</subject><subject>Epitope Mapping - methods</subject><subject>Epitopes - chemistry</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Immunology</subject><subject>Immunotherapy - methods</subject><subject>Investigations</subject><subject>Leukemia</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Molecular Sequence Data</subject><subject>Neoplasms - immunology</subject><subject>Neoplasms - therapy</subject><subject>Oncology</subject><subject>Original</subject><subject>Original Article</subject><subject>Proteins</subject><subject>Sequence Homology, Amino Acid</subject><subject>Serology</subject><subject>Tankyrases - biosynthesis</subject><subject>Tankyrases - metabolism</subject><subject>Telomerase</subject><subject>Tumors</subject><issn>0340-7004</issn><issn>1432-0851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks1u1TAQhSMEopfCA7BBFhLsAv5LnKwQqoAiVWIDa8txJr0pjh1sp-L2WXhYJspVC0gIVh55vnPGP6conjL6ilGqXidKec1LLEsquShv7hU7JgXuNBW7X-yokLRUlMqT4lFKV1hw2rYPixPWoLCt2K74cb5MxpNs_NdDNAkSMRGI6SBG47M7EPg-R0gJejJ6YoMLCC9TiAj6Hjd8NtiYFpfH2QGBecxhRpu8Nxkl_WKB7FeBcZsCnFuciWScpsUDQfM5-HXw6m-8hUhmk0fwOT0uHgzGJXhyXE-LL-_ffT47Ly8-ffh49vaitBVTuRxMP_BecGG4lVbIulW8g55VsoWKy86wtrOVGJjsFe85o11tgQmlalarprfitHiz-c5LN0FvcTYeV89xnEw86GBG_XvHj3t9Ga41Y1TQljfo8PLoEMO3BVLW05jWqxoPYUlaUYl_0db_BDlVjWhq9R9grSpereDzP8CrsESP76U5ExUTtFrHsg2yMaQUYbi9HKN6zZLesqTXcs2SvkHNs19f5U5xDA8CL46ASda4ARNjx3TLccqVZC1Hjm9cwpa_hHh3wr9P_wlPRuYD</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Shebzukhov, Yuriy V.</creator><creator>Lavrik, Inna N.</creator><creator>Karbach, Julia</creator><creator>Khlgatian, Svetlana V.</creator><creator>Koroleva, Ekaterina P.</creator><creator>Belousov, Pavel V.</creator><creator>Kashkin, Kirill N.</creator><creator>Knuth, Alexander</creator><creator>Jager, Elke</creator><creator>Chi, Nai-Wen</creator><creator>Kuprash, Dmitry V.</creator><creator>Nedospasov, Sergei A.</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080601</creationdate><title>Human tankyrases are aberrantly expressed in colon tumors and contain multiple epitopes that induce humoral and cellular immune responses in cancer patients</title><author>Shebzukhov, Yuriy V. ; Lavrik, Inna N. ; Karbach, Julia ; Khlgatian, Svetlana V. ; Koroleva, Ekaterina P. ; Belousov, Pavel V. ; Kashkin, Kirill N. ; Knuth, Alexander ; Jager, Elke ; Chi, Nai-Wen ; Kuprash, Dmitry V. ; Nedospasov, Sergei A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-fadf2d323a2c4c346972bed1549e524ba19bc53f14d72d210b6ce137761678dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amino Acid Sequence</topic><topic>Antibody Formation</topic><topic>Antigens</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Cancer</topic><topic>Cancer Research</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cell division</topic><topic>Colon</topic><topic>Colonic Neoplasms - enzymology</topic><topic>Colonic Neoplasms - pathology</topic><topic>Enzymes</topic><topic>Epitope Mapping - methods</topic><topic>Epitopes - chemistry</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Immunology</topic><topic>Immunotherapy - methods</topic><topic>Investigations</topic><topic>Leukemia</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Molecular Sequence Data</topic><topic>Neoplasms - immunology</topic><topic>Neoplasms - therapy</topic><topic>Oncology</topic><topic>Original</topic><topic>Original Article</topic><topic>Proteins</topic><topic>Sequence Homology, Amino Acid</topic><topic>Serology</topic><topic>Tankyrases - biosynthesis</topic><topic>Tankyrases - metabolism</topic><topic>Telomerase</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shebzukhov, Yuriy V.</creatorcontrib><creatorcontrib>Lavrik, Inna N.</creatorcontrib><creatorcontrib>Karbach, Julia</creatorcontrib><creatorcontrib>Khlgatian, Svetlana V.</creatorcontrib><creatorcontrib>Koroleva, Ekaterina P.</creatorcontrib><creatorcontrib>Belousov, Pavel V.</creatorcontrib><creatorcontrib>Kashkin, Kirill N.</creatorcontrib><creatorcontrib>Knuth, Alexander</creatorcontrib><creatorcontrib>Jager, Elke</creatorcontrib><creatorcontrib>Chi, Nai-Wen</creatorcontrib><creatorcontrib>Kuprash, Dmitry V.</creatorcontrib><creatorcontrib>Nedospasov, Sergei A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer Immunology, Immunotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shebzukhov, Yuriy V.</au><au>Lavrik, Inna N.</au><au>Karbach, Julia</au><au>Khlgatian, Svetlana V.</au><au>Koroleva, Ekaterina P.</au><au>Belousov, Pavel V.</au><au>Kashkin, Kirill N.</au><au>Knuth, Alexander</au><au>Jager, Elke</au><au>Chi, Nai-Wen</au><au>Kuprash, Dmitry V.</au><au>Nedospasov, Sergei A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human tankyrases are aberrantly expressed in colon tumors and contain multiple epitopes that induce humoral and cellular immune responses in cancer patients</atitle><jtitle>Cancer Immunology, Immunotherapy</jtitle><stitle>Cancer Immunol Immunother</stitle><addtitle>Cancer Immunol Immunother</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>57</volume><issue>6</issue><spage>871</spage><epage>881</epage><pages>871-881</pages><issn>0340-7004</issn><eissn>1432-0851</eissn><coden>CIIMDN</coden><abstract>Purpose
Tankyrases 1 and 2 are telomere-associated poly(ADP-ribose) polymerases (PARP) that can positively regulate telomere elongation and interact with multiple cellular proteins. Recent reports implicated tankyrases as tumor antigens and potential targets of anticancer treatment. We examined expression of tankyrases in colon tumors and immune response to these enzymes in patients with different types of cancer.
Methods
mRNA and protein expression was evaluated by quantitative real-time RT-PCR and Western blotting, respectively. Humoral immune response to recombinant tankyrases was investigated by modified enzyme-linked immunoassays. Cellular immune response was analysed by ELISPOT and
51
Cr release assays.
Results
We found that both mRNA and protein levels of tankyrase 2 (TNKL) are upregulated in colon tumors. In contrast, protein level of tankyrase 1 (TNKS) is downregulated, while mRNA level shows variable changes. More than a quarter of colon cancer patients develop humoral immune response to at least one of the two tankyrases. In this study we mapped common and unique B-cell epitopes located in different domains of the two proteins. Additionally, we present evidence for T-cell responses both to epitopes that are unique for TNKL and to those shared between TNKL and TNKS.
Conclusion
Our study favors a biomarker usage of antibody response to tankyrases. Spontaneous CD8
+
T-cell responses to these enzymes are rare and further investigation is needed to evaluate tankyrases as potential targets for cancer immunotherapy.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18026951</pmid><doi>10.1007/s00262-007-0423-z</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cancer Immunology, Immunotherapy, 2008-06, Vol.57 (6), p.871-881 |
| issn | 0340-7004 1432-0851 |
| language | eng |
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| source | MEDLINE; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Amino Acid Sequence Antibody Formation Antigens Biomarkers, Tumor - metabolism Cancer Cancer Research CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism Cell division Colon Colonic Neoplasms - enzymology Colonic Neoplasms - pathology Enzymes Epitope Mapping - methods Epitopes - chemistry Humans Immunity, Cellular Immunology Immunotherapy - methods Investigations Leukemia Medicine Medicine & Public Health Molecular Sequence Data Neoplasms - immunology Neoplasms - therapy Oncology Original Original Article Proteins Sequence Homology, Amino Acid Serology Tankyrases - biosynthesis Tankyrases - metabolism Telomerase Tumors |
| title | Human tankyrases are aberrantly expressed in colon tumors and contain multiple epitopes that induce humoral and cellular immune responses in cancer patients |
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