An introduction to mass cytometry: fundamentals and applications
Mass cytometry addresses the analytical challenges of polychromatic flow cytometry by using metal atoms as tags rather than fluorophores and atomic mass spectrometry as the detector rather than photon optics. The many available enriched stable isotopes of the transition elements can provide up to 10...
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| Veröffentlicht in: | Cancer Immunology, Immunotherapy Immunotherapy, 2013-05, Vol.62 (5), p.955-965 |
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| container_title | Cancer Immunology, Immunotherapy |
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| creator | Tanner, Scott D. Baranov, Vladimir I. Ornatsky, Olga I. Bandura, Dmitry R. George, Thaddeus C. |
| description | Mass cytometry addresses the analytical challenges of polychromatic flow cytometry by using metal atoms as tags rather than fluorophores and atomic mass spectrometry as the detector rather than photon optics. The many available enriched stable isotopes of the transition elements can provide up to 100 distinguishable reporting tags, which can be measured simultaneously because of the essential independence of detection provided by the mass spectrometer. We discuss the adaptation of traditional inductively coupled plasma mass spectrometry to cytometry applications. We focus on the generation of cytometry-compatible data and on approaches to unsupervised multivariate clustering analysis. Finally, we provide a high-level review of some recent benchmark reports that highlight the potential for massively multi-parameter mass cytometry. |
| doi_str_mv | 10.1007/s00262-013-1416-8 |
| format | Article |
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| subjects | Cancer Research Cell Separation - methods Cluster Analysis Computational Biology - methods Equipment Design Experiments Flow Cytometry - methods Fluorescent Dyes Focussed Research Review Hematopoiesis Humans Immunologic Memory Immunology Isotopes - chemistry Leukocytes, Mononuclear - cytology Mass spectrometry Mass Spectrometry - methods Medical research Medicine Medicine & Public Health Metals Molecular Weight Multivariate Analysis Neural Networks, Computer Oncology Studies T-Lymphocytes - cytology |
| title | An introduction to mass cytometry: fundamentals and applications |
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