Age-associated impairment of antitumor immunity in carcinoma-bearing mice and restoration by oral administration of Lentinula edodes mycelia extract
Because cancer is associated with aging, immunological features in the aged should be considered in anticancer immunotherapy. In this study, we investigated antitumor immunity in aged mice using a CT26 colon carcinoma model. The tumor growth of CT26 was accelerated in aged mice compared with that in...
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description | Because cancer is associated with aging, immunological features in the aged should be considered in anticancer immunotherapy. In this study, we investigated antitumor immunity in aged mice using a CT26 colon carcinoma model. The tumor growth of CT26 was accelerated in aged mice compared with that in young mice, but this difference was not observed in nude mice. The serum levels of IL-6 and TNF-α were higher in aged mice than those in young mice, irrespective of the CT26-bearing state. The in vitro induction of CT26-specific CTLs from aged mice that were vaccinated with doxorubicin (DTX)-treated CT26 cells was impaired. In vivo neutralization of IL-6, but not TNF-α, showed a tendency to restore the in vitro induction of CT26-specific CTLs from vaccinated aged mice. Analyses on tumor-infiltrating immune cells as early as day 5 after CT26 inoculation revealed that monocytic and granulocytic MDSCs preferentially infiltrated into tumor sites in aged mice compared with young mice. Alternatively, oral administration of
Lentinula edodes
mycelia (L.E.M.) extract, which has the potential to suppress inflammation in tumor-bearing hosts, decreased the serum levels of IL-6 in aged mice. When administration of L.E.M. extract was started 1 week earlier, CT26 growth was retarded in aged mice and the in vivo priming of tumor-specific CTLs was improved in CT26-vaccinated aged mice. These results indicate early infiltration of MDSCs is related to impaired immunity of aged hosts and that oral administration of L.E.M. extract can mitigate the impairment. |
doi_str_mv | 10.1007/s00262-016-1857-y |
format | Article |
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Lentinula edodes
mycelia (L.E.M.) extract, which has the potential to suppress inflammation in tumor-bearing hosts, decreased the serum levels of IL-6 in aged mice. When administration of L.E.M. extract was started 1 week earlier, CT26 growth was retarded in aged mice and the in vivo priming of tumor-specific CTLs was improved in CT26-vaccinated aged mice. These results indicate early infiltration of MDSCs is related to impaired immunity of aged hosts and that oral administration of L.E.M. extract can mitigate the impairment.</description><identifier>ISSN: 0340-7004</identifier><identifier>EISSN: 1432-0851</identifier><identifier>DOI: 10.1007/s00262-016-1857-y</identifier><identifier>PMID: 27312060</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Administration, Oral ; Age Factors ; Aging ; Animals ; Cancer ; Cancer Research ; Cell Line, Tumor ; Colonic Neoplasms - immunology ; Cytokines ; Drug dosages ; Female ; Humans ; Immunology ; Immunotherapy ; Lentinula edodes ; Lymphocytes ; Medicine ; Medicine & Public Health ; Melanoma ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Oncology ; Oral administration ; Original ; Original Article ; Peptides ; Reagents ; Shiitake Mushrooms - metabolism ; Shiitake Mushrooms - therapeutic use ; University faculty ; Vaccines</subject><ispartof>Cancer Immunology, Immunotherapy, 2016-08, Vol.65 (8), p.961-972</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c614t-9e4c43346a932c36882e6e3fc3c4f11b765fb847225c739cc6146a11251a013d3</citedby><cites>FETCH-LOGICAL-c614t-9e4c43346a932c36882e6e3fc3c4f11b765fb847225c739cc6146a11251a013d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11028864/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11028864/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,41493,42562,51324,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27312060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishikawa, Satoru</creatorcontrib><creatorcontrib>Matsui, Yasunori</creatorcontrib><creatorcontrib>Wachi, Satoshi</creatorcontrib><creatorcontrib>Yamaguchi, Hiroshi</creatorcontrib><creatorcontrib>Harashima, Nanae</creatorcontrib><creatorcontrib>Harada, Mamoru</creatorcontrib><title>Age-associated impairment of antitumor immunity in carcinoma-bearing mice and restoration by oral administration of Lentinula edodes mycelia extract</title><title>Cancer Immunology, Immunotherapy</title><addtitle>Cancer Immunol Immunother</addtitle><addtitle>Cancer Immunol Immunother</addtitle><description>Because cancer is associated with aging, immunological features in the aged should be considered in anticancer immunotherapy. In this study, we investigated antitumor immunity in aged mice using a CT26 colon carcinoma model. The tumor growth of CT26 was accelerated in aged mice compared with that in young mice, but this difference was not observed in nude mice. The serum levels of IL-6 and TNF-α were higher in aged mice than those in young mice, irrespective of the CT26-bearing state. The in vitro induction of CT26-specific CTLs from aged mice that were vaccinated with doxorubicin (DTX)-treated CT26 cells was impaired. In vivo neutralization of IL-6, but not TNF-α, showed a tendency to restore the in vitro induction of CT26-specific CTLs from vaccinated aged mice. Analyses on tumor-infiltrating immune cells as early as day 5 after CT26 inoculation revealed that monocytic and granulocytic MDSCs preferentially infiltrated into tumor sites in aged mice compared with young mice. Alternatively, oral administration of
Lentinula edodes
mycelia (L.E.M.) extract, which has the potential to suppress inflammation in tumor-bearing hosts, decreased the serum levels of IL-6 in aged mice. When administration of L.E.M. extract was started 1 week earlier, CT26 growth was retarded in aged mice and the in vivo priming of tumor-specific CTLs was improved in CT26-vaccinated aged mice. These results indicate early infiltration of MDSCs is related to impaired immunity of aged hosts and that oral administration of L.E.M. extract can mitigate the impairment.</description><subject>Administration, Oral</subject><subject>Age Factors</subject><subject>Aging</subject><subject>Animals</subject><subject>Cancer</subject><subject>Cancer Research</subject><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms - immunology</subject><subject>Cytokines</subject><subject>Drug dosages</subject><subject>Female</subject><subject>Humans</subject><subject>Immunology</subject><subject>Immunotherapy</subject><subject>Lentinula edodes</subject><subject>Lymphocytes</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Melanoma</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Oncology</subject><subject>Oral administration</subject><subject>Original</subject><subject>Original Article</subject><subject>Peptides</subject><subject>Reagents</subject><subject>Shiitake Mushrooms - metabolism</subject><subject>Shiitake Mushrooms - therapeutic use</subject><subject>University faculty</subject><subject>Vaccines</subject><issn>0340-7004</issn><issn>1432-0851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks-OFCEQxonRuOPqA3gxJF68tFJA090ns9n4L5nEi54JTdMjmwFGoI39Hj6wNc64WU1MPFEUv_qogo-Qp8BeAmPdq8IYV7xhoBro265Z75ENSIGZvoX7ZMOEZE3HmLwgj0q5wYCzYXhILngngDPFNuTH1c41ppRkvaluoj4cjM_BxUrTTE2svi4hZcyHJfq6Uh-pNdn6mIJpRmeyjzsavHUITzS7UlM21adIx5ViuKdmCj76Us9plN2ivI_L3lA3pckVGlbr9h6335Gy9TF5MJt9cU_O6yX5_PbNp-v3zfbjuw_XV9vGKpC1GZy0UgipzCC4FarvuVNOzFZYOQOMnWrnsZcd563txGCPVcoA8BYMAzGJS_L6pHtYxuAmi21hw_qQfTB51cl4_edJ9F_0Ln3TAIz3vZKo8OKskNPXBYfXwRecZW-iS0vR0AMMDKDj_4EyJaWQg0D0-V_oTVpyxKf4JQhC9HxACk6UzamU7ObbxoHpoz_0yR8a_aGP_tAr1jy7O_FtxW9DIMBPQDkcf9blO1f_U_UnSN3I6g</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Ishikawa, Satoru</creator><creator>Matsui, Yasunori</creator><creator>Wachi, Satoshi</creator><creator>Yamaguchi, Hiroshi</creator><creator>Harashima, Nanae</creator><creator>Harada, Mamoru</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20160801</creationdate><title>Age-associated impairment of antitumor immunity in carcinoma-bearing mice and restoration by oral administration of Lentinula edodes mycelia extract</title><author>Ishikawa, Satoru ; Matsui, Yasunori ; Wachi, Satoshi ; Yamaguchi, Hiroshi ; Harashima, Nanae ; Harada, Mamoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c614t-9e4c43346a932c36882e6e3fc3c4f11b765fb847225c739cc6146a11251a013d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Administration, Oral</topic><topic>Age Factors</topic><topic>Aging</topic><topic>Animals</topic><topic>Cancer</topic><topic>Cancer Research</topic><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms - immunology</topic><topic>Cytokines</topic><topic>Drug dosages</topic><topic>Female</topic><topic>Humans</topic><topic>Immunology</topic><topic>Immunotherapy</topic><topic>Lentinula edodes</topic><topic>Lymphocytes</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Melanoma</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Oncology</topic><topic>Oral administration</topic><topic>Original</topic><topic>Original Article</topic><topic>Peptides</topic><topic>Reagents</topic><topic>Shiitake Mushrooms - metabolism</topic><topic>Shiitake Mushrooms - therapeutic use</topic><topic>University faculty</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishikawa, Satoru</creatorcontrib><creatorcontrib>Matsui, Yasunori</creatorcontrib><creatorcontrib>Wachi, Satoshi</creatorcontrib><creatorcontrib>Yamaguchi, Hiroshi</creatorcontrib><creatorcontrib>Harashima, Nanae</creatorcontrib><creatorcontrib>Harada, Mamoru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer Immunology, Immunotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishikawa, Satoru</au><au>Matsui, Yasunori</au><au>Wachi, Satoshi</au><au>Yamaguchi, Hiroshi</au><au>Harashima, Nanae</au><au>Harada, Mamoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-associated impairment of antitumor immunity in carcinoma-bearing mice and restoration by oral administration of Lentinula edodes mycelia extract</atitle><jtitle>Cancer Immunology, Immunotherapy</jtitle><stitle>Cancer Immunol Immunother</stitle><addtitle>Cancer Immunol Immunother</addtitle><date>2016-08-01</date><risdate>2016</risdate><volume>65</volume><issue>8</issue><spage>961</spage><epage>972</epage><pages>961-972</pages><issn>0340-7004</issn><eissn>1432-0851</eissn><abstract>Because cancer is associated with aging, immunological features in the aged should be considered in anticancer immunotherapy. In this study, we investigated antitumor immunity in aged mice using a CT26 colon carcinoma model. The tumor growth of CT26 was accelerated in aged mice compared with that in young mice, but this difference was not observed in nude mice. The serum levels of IL-6 and TNF-α were higher in aged mice than those in young mice, irrespective of the CT26-bearing state. The in vitro induction of CT26-specific CTLs from aged mice that were vaccinated with doxorubicin (DTX)-treated CT26 cells was impaired. In vivo neutralization of IL-6, but not TNF-α, showed a tendency to restore the in vitro induction of CT26-specific CTLs from vaccinated aged mice. Analyses on tumor-infiltrating immune cells as early as day 5 after CT26 inoculation revealed that monocytic and granulocytic MDSCs preferentially infiltrated into tumor sites in aged mice compared with young mice. Alternatively, oral administration of
Lentinula edodes
mycelia (L.E.M.) extract, which has the potential to suppress inflammation in tumor-bearing hosts, decreased the serum levels of IL-6 in aged mice. When administration of L.E.M. extract was started 1 week earlier, CT26 growth was retarded in aged mice and the in vivo priming of tumor-specific CTLs was improved in CT26-vaccinated aged mice. These results indicate early infiltration of MDSCs is related to impaired immunity of aged hosts and that oral administration of L.E.M. extract can mitigate the impairment.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27312060</pmid><doi>10.1007/s00262-016-1857-y</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Administration, Oral Age Factors Aging Animals Cancer Cancer Research Cell Line, Tumor Colonic Neoplasms - immunology Cytokines Drug dosages Female Humans Immunology Immunotherapy Lentinula edodes Lymphocytes Medicine Medicine & Public Health Melanoma Mice Mice, Inbred BALB C Mice, Nude Oncology Oral administration Original Original Article Peptides Reagents Shiitake Mushrooms - metabolism Shiitake Mushrooms - therapeutic use University faculty Vaccines |
title | Age-associated impairment of antitumor immunity in carcinoma-bearing mice and restoration by oral administration of Lentinula edodes mycelia extract |
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