Direct Reprogramming of Somatic Skin Cells from a Patient with Huntington’s Disease into Striatal Neurons to Create Models of Pathology
A new in vitro model of Huntington’s disease (HD) was developed via a direct reprogramming of dermal fibroblasts from HD patients into striatal neurons. A reprogramming into induced pluripotent stem (iPS) cells is obviated in the case of direct reprogramming, which thus yields neurons that preserve...
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| Veröffentlicht in: | Doklady. Biological sciences 2024-04, Vol.515 (1), p.15-19 |
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| creator | Kraskovskaya, N. A. Khotin, M. G. Tomilin, A. N. Mikhailova, N. A. |
| description | A new in vitro model of Huntington’s disease (HD) was developed via a direct reprogramming of dermal fibroblasts from HD patients into striatal neurons. A reprogramming into induced pluripotent stem (iPS) cells is obviated in the case of direct reprogramming, which thus yields neurons that preserve the epigenetic information inherent in cells of a particular donor and, consequently, the age-associated disease phenotype. A main histopathological feature of HD was reproduced in the new model; i.e., aggregates of mutant huntingtin accumulated in striatal neurons derived from a patient’s fibroblasts. Experiments with cultured neurons obtained via direct reprogramming make it possible to individually assess the progression of neuropathology and to implement a personalized approach to choosing the treatment strategy and drugs for therapy. The in vitro model of HD can be used in preclinical drug studies. |
| doi_str_mv | 10.1134/S0012496623700849 |
| format | Article |
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Experiments with cultured neurons obtained via direct reprogramming make it possible to individually assess the progression of neuropathology and to implement a personalized approach to choosing the treatment strategy and drugs for therapy. 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| subjects | Animals Biomedical and Life Sciences Corpus Striatum - pathology Disease Models, Animal Epigenetics Evolutionary Biology Fibroblasts Humans Huntingtin Huntington Disease - genetics Huntington Disease - pathology Huntington's disease Huntingtons disease Induced Pluripotent Stem Cells - pathology Life Sciences Neostriatum Neurons Phenotypes Plant Sciences Pluripotency |
| title | Direct Reprogramming of Somatic Skin Cells from a Patient with Huntington’s Disease into Striatal Neurons to Create Models of Pathology |
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