Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil
is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61 spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38....
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2024-04, Vol.68 (4), p.e0162023 |
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| creator | Ribeiro Dos Santos, Amanda Gade, Lalitha Misas, Elizabeth Litvintseva, Anastasia P Nunnally, Natalie S Parnell, Lindsay A Rajeev, Malavika de Souza Carvalho Melhem, Marcia Takahashi, Juliana Possato Fernandes Oliboni, Gabriel Manzi Bonfieti, Lucas Xavier Araujo, Lisandra Siufi Cappellano, Paola Venturini, James Lockhart, Shawn R Sexton, D Joseph |
| description | is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61
spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of
isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the
resistant isolates were not clonal and were distributed across three different
clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of
resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene,
, corresponding to the well-known G448S substitution inducing azole resistance in
. SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26
resistant isolates. |
| doi_str_mv | 10.1128/aac.01620-23 |
| format | Article |
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spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of
isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the
resistant isolates were not clonal and were distributed across three different
clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of
resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene,
, corresponding to the well-known G448S substitution inducing azole resistance in
. SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26
resistant isolates.</description><identifier>ISSN: 0066-4804</identifier><identifier>ISSN: 1098-6596</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/aac.01620-23</identifier><identifier>PMID: 38385701</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Antimicrobial Chemotherapy ; Susceptibility</subject><ispartof>Antimicrobial agents and chemotherapy, 2024-04, Vol.68 (4), p.e0162023</ispartof><rights>This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a376t-2b2e88d69352be68352b0bce17e44245f8c2a734e9c97bff5b98aa1127167e0c3</cites><orcidid>0000-0003-3099-7334 ; 0000-0002-2551-015X ; 0000-0002-5577-4498 ; 0000-0003-1086-8041 ; 0000-0002-4383-5994 ; 0000-0001-6243-7716</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989022/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989022/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38385701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Krysan, Damian J.</contributor><creatorcontrib>Ribeiro Dos Santos, Amanda</creatorcontrib><creatorcontrib>Gade, Lalitha</creatorcontrib><creatorcontrib>Misas, Elizabeth</creatorcontrib><creatorcontrib>Litvintseva, Anastasia P</creatorcontrib><creatorcontrib>Nunnally, Natalie S</creatorcontrib><creatorcontrib>Parnell, Lindsay A</creatorcontrib><creatorcontrib>Rajeev, Malavika</creatorcontrib><creatorcontrib>de Souza Carvalho Melhem, Marcia</creatorcontrib><creatorcontrib>Takahashi, Juliana Possato Fernandes</creatorcontrib><creatorcontrib>Oliboni, Gabriel Manzi</creatorcontrib><creatorcontrib>Bonfieti, Lucas Xavier</creatorcontrib><creatorcontrib>Araujo, Lisandra Siufi</creatorcontrib><creatorcontrib>Cappellano, Paola</creatorcontrib><creatorcontrib>Venturini, James</creatorcontrib><creatorcontrib>Lockhart, Shawn R</creatorcontrib><creatorcontrib>Sexton, D Joseph</creatorcontrib><title>Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil</title><title>Antimicrobial agents and chemotherapy</title><addtitle>Antimicrob Agents Chemother</addtitle><addtitle>Antimicrob Agents Chemother</addtitle><description>is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61
spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of
isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the
resistant isolates were not clonal and were distributed across three different
clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of
resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene,
, corresponding to the well-known G448S substitution inducing azole resistance in
. SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26
resistant isolates.</description><subject>Antimicrobial Chemotherapy</subject><subject>Susceptibility</subject><issn>0066-4804</issn><issn>1098-6596</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kc1LxDAQxYMouq7ePEuPClbz0abJSVzxCwQP6sVLmHRTjaTNmqSi_vV23VX04GmYmR9vePMQ2iH4kBAqjgDqQ0w4xTllK2hEsBQ5LyVfRSOMOc8LgYsNtBnjMx76UuJ1tMEEE2WFyQg9TGzrp-CyqY0pWN0n67vMNxl8eGey2MfazJLV1tn0ntkuu5354NNTsG-ZDhCHuemijZmN3kEycc5MAnxYt4XWGnDRbC_rGN2fn92dXubXNxdXpyfXObCKp5xqaoSYcslKqg0X84J1bUhlioIWZSNqChUrjKxlpZum1FIADNYrwiuDazZGxwvdWa9bM61NlwI4NQu2hfCuPFj1d9PZJ_XoX9X8VRJTOijsLRWCf-lNTKq1g2_noDO-j4pKhouKMsoG9GCB1sHHGEzzc4dgNc9DDXmorzzUF76_wCG2VD37PnTDK_5jd3_7-BH-Dot9AqzMlY0</recordid><startdate>20240403</startdate><enddate>20240403</enddate><creator>Ribeiro Dos Santos, Amanda</creator><creator>Gade, Lalitha</creator><creator>Misas, Elizabeth</creator><creator>Litvintseva, Anastasia P</creator><creator>Nunnally, Natalie S</creator><creator>Parnell, Lindsay A</creator><creator>Rajeev, Malavika</creator><creator>de Souza Carvalho Melhem, Marcia</creator><creator>Takahashi, Juliana Possato Fernandes</creator><creator>Oliboni, Gabriel Manzi</creator><creator>Bonfieti, Lucas Xavier</creator><creator>Araujo, Lisandra Siufi</creator><creator>Cappellano, Paola</creator><creator>Venturini, James</creator><creator>Lockhart, Shawn R</creator><creator>Sexton, D Joseph</creator><general>American Society for Microbiology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3099-7334</orcidid><orcidid>https://orcid.org/0000-0002-2551-015X</orcidid><orcidid>https://orcid.org/0000-0002-5577-4498</orcidid><orcidid>https://orcid.org/0000-0003-1086-8041</orcidid><orcidid>https://orcid.org/0000-0002-4383-5994</orcidid><orcidid>https://orcid.org/0000-0001-6243-7716</orcidid></search><sort><creationdate>20240403</creationdate><title>Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil</title><author>Ribeiro Dos Santos, Amanda ; 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Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61
spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of
isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the
resistant isolates were not clonal and were distributed across three different
clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of
resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene,
, corresponding to the well-known G448S substitution inducing azole resistance in
. SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26
resistant isolates.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>38385701</pmid><doi>10.1128/aac.01620-23</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3099-7334</orcidid><orcidid>https://orcid.org/0000-0002-2551-015X</orcidid><orcidid>https://orcid.org/0000-0002-5577-4498</orcidid><orcidid>https://orcid.org/0000-0003-1086-8041</orcidid><orcidid>https://orcid.org/0000-0002-4383-5994</orcidid><orcidid>https://orcid.org/0000-0001-6243-7716</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Antimicrobial agents and chemotherapy, 2024-04, Vol.68 (4), p.e0162023 |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antimicrobial Chemotherapy Susceptibility |
| title | Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil |
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