Short-Term Storage of Mobilized Peripheral Blood Stem Cells in a Closed System Changes the Microenvironment and May Affect the Quantity of CD34+ and CD34+CD38-CD45RA-CD90+ Cells
•Storage of peripheral blood stem cell samples in airtight blood collection tubes results in a hypoxic microenvironment.•The concentrations of CD34+, CD34+CD38-, CD34+CD38+, and CD34+CD38-CD45RA-CD90+ immunophenotypically defined stem cells were increased after 8 hours of storage in airtight blood c...
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| Veröffentlicht in: | Transplantation and cellular therapy 2023-02, Vol.29 (2), p.112.e1-112.e9 |
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| creator | Elshoury, Amro Maguire, Orla Conway, Alexis Tario, Joseph Soh, Kah Teong Ross, Maureen Hahn, Theresa Becker, Joanne Wallace, Paul McCarthy, Philip L. Minderman, Hans Chen, George L. |
| description | •Storage of peripheral blood stem cell samples in airtight blood collection tubes results in a hypoxic microenvironment.•The concentrations of CD34+, CD34+CD38-, CD34+CD38+, and CD34+CD38-CD45RA-CD90+ immunophenotypically defined stem cells were increased after 8 hours of storage in airtight blood collection tubes.
Hypoxic conditions preserve the multipotency and self-renewing capacity of murine bone marrow and human cord blood stem cells. Blood samples stored in sealed blood gas tubes become hypoxic as leukocytes metabolize and consume oxygen. Taken together, these observations suggest that peripheral blood stem cell (PBSC) samples stored under airtight conditions become hypoxic, and that the stem cells contained may undergo qualitative or quantitative changes. This study aimed to determine the effect of storage for 8 hours in a sealed system on PBSC samples. Granulocyte colony-stimulating factor-mobilized PBSC samples were collected prospectively from 9 patients with myeloma or amyloidosis prior to apheresis, followed by measurement of CO2, O2, hydrogen ion (pH), lactate, and glucose concentrations in the blood and immunophenotyping of stem cell and multipotent progenitor cell populations before and after 8 hours of storage in sealed blood collection tubes. Blood concentrations of O2 and glucose and pH measurements were significantly decreased, whereas concentrations of CO2 and lactate were significantly increased after storage. Significantly higher concentrations of CD34+ cells (552 ± 84 cells/106 total nucleated cells [TNCs] versus 985 ± 143 cells/106 TNCs; P = .03), CD34+CD38- cells (98 ± 32 cells/106 TNCs versus 158 ± 52 cells/106 TNCs; P = .03), CD34+CD38+ cells (444 ± 92 cells/106 TNCs versus 789 ± 153 cells/106 TNCs; P = .03), and CD34+CD38-CD45RA-CD90+ cells (55 ± 17 cells/106 TNCs versus 89 ± 25 cells/106 TNCs; P = .02) were detected after 8 hours of storage. The changes in concentrations of CD34+CD38+ cells and CD34+ cells were inversely associated with the change in glucose concentration (P = .003 and P < .001, respectively) and positively associated with the change in lactate concentration (P = .01 and P |
| doi_str_mv | 10.1016/j.jtct.2022.11.021 |
| format | Article |
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Hypoxic conditions preserve the multipotency and self-renewing capacity of murine bone marrow and human cord blood stem cells. Blood samples stored in sealed blood gas tubes become hypoxic as leukocytes metabolize and consume oxygen. Taken together, these observations suggest that peripheral blood stem cell (PBSC) samples stored under airtight conditions become hypoxic, and that the stem cells contained may undergo qualitative or quantitative changes. This study aimed to determine the effect of storage for 8 hours in a sealed system on PBSC samples. Granulocyte colony-stimulating factor-mobilized PBSC samples were collected prospectively from 9 patients with myeloma or amyloidosis prior to apheresis, followed by measurement of CO2, O2, hydrogen ion (pH), lactate, and glucose concentrations in the blood and immunophenotyping of stem cell and multipotent progenitor cell populations before and after 8 hours of storage in sealed blood collection tubes. Blood concentrations of O2 and glucose and pH measurements were significantly decreased, whereas concentrations of CO2 and lactate were significantly increased after storage. Significantly higher concentrations of CD34+ cells (552 ± 84 cells/106 total nucleated cells [TNCs] versus 985 ± 143 cells/106 TNCs; P = .03), CD34+CD38- cells (98 ± 32 cells/106 TNCs versus 158 ± 52 cells/106 TNCs; P = .03), CD34+CD38+ cells (444 ± 92 cells/106 TNCs versus 789 ± 153 cells/106 TNCs; P = .03), and CD34+CD38-CD45RA-CD90+ cells (55 ± 17 cells/106 TNCs versus 89 ± 25 cells/106 TNCs; P = .02) were detected after 8 hours of storage. The changes in concentrations of CD34+CD38+ cells and CD34+ cells were inversely associated with the change in glucose concentration (P = .003 and P < .001, respectively) and positively associated with the change in lactate concentration (P = .01 and P <.001, respectively) after 8 hours of airtight storage. Storage of PBSC samples in a sealed, airtight environment is associated with microenvironmental changes consistent with hypoxia and increased concentrations of immunophenotypically defined stem cells. These results may have clinical implications with regard to the collection and processing of stem cell products and warrant confirmation with functional and mechanistic studies.</description><identifier>ISSN: 2666-6367</identifier><identifier>ISSN: 2666-6375</identifier><identifier>EISSN: 2666-6367</identifier><identifier>DOI: 10.1016/j.jtct.2022.11.021</identifier><identifier>PMID: 36436782</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antigens, CD34 - metabolism ; Carbon Dioxide ; Cell Adhesion Molecules ; Graft collection ; Graft processing ; Hematopoiesis ; Humans ; Hypoxia ; Lactates ; Leukocyte Common Antigens - metabolism ; Mice ; Peripheral Blood Stem Cells - metabolism ; Thy-1 Antigens - metabolism</subject><ispartof>Transplantation and cellular therapy, 2023-02, Vol.29 (2), p.112.e1-112.e9</ispartof><rights>2022 The American Society for Transplantation and Cellular Therapy</rights><rights>Copyright © 2022 The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c337t-1f216721279138ec96b4c369c5a33f0e31445508c3de37beca4fd5054d81bd173</cites><orcidid>0000-0002-3835-8855 ; 0000-0002-7036-2109</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36436782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elshoury, Amro</creatorcontrib><creatorcontrib>Maguire, Orla</creatorcontrib><creatorcontrib>Conway, Alexis</creatorcontrib><creatorcontrib>Tario, Joseph</creatorcontrib><creatorcontrib>Soh, Kah Teong</creatorcontrib><creatorcontrib>Ross, Maureen</creatorcontrib><creatorcontrib>Hahn, Theresa</creatorcontrib><creatorcontrib>Becker, Joanne</creatorcontrib><creatorcontrib>Wallace, Paul</creatorcontrib><creatorcontrib>McCarthy, Philip L.</creatorcontrib><creatorcontrib>Minderman, Hans</creatorcontrib><creatorcontrib>Chen, George L.</creatorcontrib><title>Short-Term Storage of Mobilized Peripheral Blood Stem Cells in a Closed System Changes the Microenvironment and May Affect the Quantity of CD34+ and CD34+CD38-CD45RA-CD90+ Cells</title><title>Transplantation and cellular therapy</title><addtitle>Transplant Cell Ther</addtitle><description>•Storage of peripheral blood stem cell samples in airtight blood collection tubes results in a hypoxic microenvironment.•The concentrations of CD34+, CD34+CD38-, CD34+CD38+, and CD34+CD38-CD45RA-CD90+ immunophenotypically defined stem cells were increased after 8 hours of storage in airtight blood collection tubes.
Hypoxic conditions preserve the multipotency and self-renewing capacity of murine bone marrow and human cord blood stem cells. Blood samples stored in sealed blood gas tubes become hypoxic as leukocytes metabolize and consume oxygen. Taken together, these observations suggest that peripheral blood stem cell (PBSC) samples stored under airtight conditions become hypoxic, and that the stem cells contained may undergo qualitative or quantitative changes. This study aimed to determine the effect of storage for 8 hours in a sealed system on PBSC samples. Granulocyte colony-stimulating factor-mobilized PBSC samples were collected prospectively from 9 patients with myeloma or amyloidosis prior to apheresis, followed by measurement of CO2, O2, hydrogen ion (pH), lactate, and glucose concentrations in the blood and immunophenotyping of stem cell and multipotent progenitor cell populations before and after 8 hours of storage in sealed blood collection tubes. Blood concentrations of O2 and glucose and pH measurements were significantly decreased, whereas concentrations of CO2 and lactate were significantly increased after storage. Significantly higher concentrations of CD34+ cells (552 ± 84 cells/106 total nucleated cells [TNCs] versus 985 ± 143 cells/106 TNCs; P = .03), CD34+CD38- cells (98 ± 32 cells/106 TNCs versus 158 ± 52 cells/106 TNCs; P = .03), CD34+CD38+ cells (444 ± 92 cells/106 TNCs versus 789 ± 153 cells/106 TNCs; P = .03), and CD34+CD38-CD45RA-CD90+ cells (55 ± 17 cells/106 TNCs versus 89 ± 25 cells/106 TNCs; P = .02) were detected after 8 hours of storage. The changes in concentrations of CD34+CD38+ cells and CD34+ cells were inversely associated with the change in glucose concentration (P = .003 and P < .001, respectively) and positively associated with the change in lactate concentration (P = .01 and P <.001, respectively) after 8 hours of airtight storage. Storage of PBSC samples in a sealed, airtight environment is associated with microenvironmental changes consistent with hypoxia and increased concentrations of immunophenotypically defined stem cells. These results may have clinical implications with regard to the collection and processing of stem cell products and warrant confirmation with functional and mechanistic studies.</description><subject>Animals</subject><subject>Antigens, CD34 - metabolism</subject><subject>Carbon Dioxide</subject><subject>Cell Adhesion Molecules</subject><subject>Graft collection</subject><subject>Graft processing</subject><subject>Hematopoiesis</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Lactates</subject><subject>Leukocyte Common Antigens - metabolism</subject><subject>Mice</subject><subject>Peripheral Blood Stem Cells - metabolism</subject><subject>Thy-1 Antigens - metabolism</subject><issn>2666-6367</issn><issn>2666-6375</issn><issn>2666-6367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kduK2zAQhk1p6S7bfYFeFF0WFqc6WbahUFJvT7Chh2yvhSyPYwVbykpKIH2rvmGVZLtsb3qjGZhv_tHMn2UvCZ4RTMSb9WwddZxRTOmMkBmm5El2ToUQuWCifPooP8suQ1hjjClnmDD8PDtjgqdCRc-z38vB-Zjfgp_QMjqvVoBcjxauNaP5BR36Bt5sBvBqRO9H57pEwYQaGMeAjEUKNaMLiVvuw7EwKLuCgOIAaGG0d2B3xjs7gY1I2Q4t1B7N-x50PDLft8pGE_eHoc0141dH6Jilp8qba178mKdQ46vT1BfZs16NAS7v40X28-OH2-ZzfvP105dmfpNrxsqYk54SUVJCy5qwCnQtWq6ZqHWhGOsxMMJ5UeBKsw5Y2YJWvO8KXPCuIm1HSnaRvTvpbrbtBJ1OC6QjyI03k_J76ZSR_1asGeTK7STBtRBVzZPC63sF7-62EKKcTNBpB2XBbYOkJcc1ruqSJZSe0HSxEDz0D3MIlge_5Voe_JYHvyUhMvmdml49_uFDy193E_D2BEC6086Al0EbsBo645MBsnPmf_p_AG4euoU</recordid><startdate>20230201</startdate><enddate>20230201</enddate><creator>Elshoury, Amro</creator><creator>Maguire, Orla</creator><creator>Conway, Alexis</creator><creator>Tario, Joseph</creator><creator>Soh, Kah Teong</creator><creator>Ross, Maureen</creator><creator>Hahn, Theresa</creator><creator>Becker, Joanne</creator><creator>Wallace, Paul</creator><creator>McCarthy, Philip L.</creator><creator>Minderman, Hans</creator><creator>Chen, George L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3835-8855</orcidid><orcidid>https://orcid.org/0000-0002-7036-2109</orcidid></search><sort><creationdate>20230201</creationdate><title>Short-Term Storage of Mobilized Peripheral Blood Stem Cells in a Closed System Changes the Microenvironment and May Affect the Quantity of CD34+ and CD34+CD38-CD45RA-CD90+ Cells</title><author>Elshoury, Amro ; Maguire, Orla ; Conway, Alexis ; Tario, Joseph ; Soh, Kah Teong ; Ross, Maureen ; Hahn, Theresa ; Becker, Joanne ; Wallace, Paul ; McCarthy, Philip L. ; Minderman, Hans ; Chen, George L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-1f216721279138ec96b4c369c5a33f0e31445508c3de37beca4fd5054d81bd173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Antigens, CD34 - metabolism</topic><topic>Carbon Dioxide</topic><topic>Cell Adhesion Molecules</topic><topic>Graft collection</topic><topic>Graft processing</topic><topic>Hematopoiesis</topic><topic>Humans</topic><topic>Hypoxia</topic><topic>Lactates</topic><topic>Leukocyte Common Antigens - metabolism</topic><topic>Mice</topic><topic>Peripheral Blood Stem Cells - metabolism</topic><topic>Thy-1 Antigens - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elshoury, Amro</creatorcontrib><creatorcontrib>Maguire, Orla</creatorcontrib><creatorcontrib>Conway, Alexis</creatorcontrib><creatorcontrib>Tario, Joseph</creatorcontrib><creatorcontrib>Soh, Kah Teong</creatorcontrib><creatorcontrib>Ross, Maureen</creatorcontrib><creatorcontrib>Hahn, Theresa</creatorcontrib><creatorcontrib>Becker, Joanne</creatorcontrib><creatorcontrib>Wallace, Paul</creatorcontrib><creatorcontrib>McCarthy, Philip L.</creatorcontrib><creatorcontrib>Minderman, Hans</creatorcontrib><creatorcontrib>Chen, George L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Transplantation and cellular therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elshoury, Amro</au><au>Maguire, Orla</au><au>Conway, Alexis</au><au>Tario, Joseph</au><au>Soh, Kah Teong</au><au>Ross, Maureen</au><au>Hahn, Theresa</au><au>Becker, Joanne</au><au>Wallace, Paul</au><au>McCarthy, Philip L.</au><au>Minderman, Hans</au><au>Chen, George L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short-Term Storage of Mobilized Peripheral Blood Stem Cells in a Closed System Changes the Microenvironment and May Affect the Quantity of CD34+ and CD34+CD38-CD45RA-CD90+ Cells</atitle><jtitle>Transplantation and cellular therapy</jtitle><addtitle>Transplant Cell Ther</addtitle><date>2023-02-01</date><risdate>2023</risdate><volume>29</volume><issue>2</issue><spage>112.e1</spage><epage>112.e9</epage><pages>112.e1-112.e9</pages><issn>2666-6367</issn><issn>2666-6375</issn><eissn>2666-6367</eissn><abstract>•Storage of peripheral blood stem cell samples in airtight blood collection tubes results in a hypoxic microenvironment.•The concentrations of CD34+, CD34+CD38-, CD34+CD38+, and CD34+CD38-CD45RA-CD90+ immunophenotypically defined stem cells were increased after 8 hours of storage in airtight blood collection tubes.
Hypoxic conditions preserve the multipotency and self-renewing capacity of murine bone marrow and human cord blood stem cells. Blood samples stored in sealed blood gas tubes become hypoxic as leukocytes metabolize and consume oxygen. Taken together, these observations suggest that peripheral blood stem cell (PBSC) samples stored under airtight conditions become hypoxic, and that the stem cells contained may undergo qualitative or quantitative changes. This study aimed to determine the effect of storage for 8 hours in a sealed system on PBSC samples. Granulocyte colony-stimulating factor-mobilized PBSC samples were collected prospectively from 9 patients with myeloma or amyloidosis prior to apheresis, followed by measurement of CO2, O2, hydrogen ion (pH), lactate, and glucose concentrations in the blood and immunophenotyping of stem cell and multipotent progenitor cell populations before and after 8 hours of storage in sealed blood collection tubes. Blood concentrations of O2 and glucose and pH measurements were significantly decreased, whereas concentrations of CO2 and lactate were significantly increased after storage. Significantly higher concentrations of CD34+ cells (552 ± 84 cells/106 total nucleated cells [TNCs] versus 985 ± 143 cells/106 TNCs; P = .03), CD34+CD38- cells (98 ± 32 cells/106 TNCs versus 158 ± 52 cells/106 TNCs; P = .03), CD34+CD38+ cells (444 ± 92 cells/106 TNCs versus 789 ± 153 cells/106 TNCs; P = .03), and CD34+CD38-CD45RA-CD90+ cells (55 ± 17 cells/106 TNCs versus 89 ± 25 cells/106 TNCs; P = .02) were detected after 8 hours of storage. The changes in concentrations of CD34+CD38+ cells and CD34+ cells were inversely associated with the change in glucose concentration (P = .003 and P < .001, respectively) and positively associated with the change in lactate concentration (P = .01 and P <.001, respectively) after 8 hours of airtight storage. Storage of PBSC samples in a sealed, airtight environment is associated with microenvironmental changes consistent with hypoxia and increased concentrations of immunophenotypically defined stem cells. These results may have clinical implications with regard to the collection and processing of stem cell products and warrant confirmation with functional and mechanistic studies.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>36436782</pmid><doi>10.1016/j.jtct.2022.11.021</doi><orcidid>https://orcid.org/0000-0002-3835-8855</orcidid><orcidid>https://orcid.org/0000-0002-7036-2109</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Transplantation and cellular therapy, 2023-02, Vol.29 (2), p.112.e1-112.e9 |
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| subjects | Animals Antigens, CD34 - metabolism Carbon Dioxide Cell Adhesion Molecules Graft collection Graft processing Hematopoiesis Humans Hypoxia Lactates Leukocyte Common Antigens - metabolism Mice Peripheral Blood Stem Cells - metabolism Thy-1 Antigens - metabolism |
| title | Short-Term Storage of Mobilized Peripheral Blood Stem Cells in a Closed System Changes the Microenvironment and May Affect the Quantity of CD34+ and CD34+CD38-CD45RA-CD90+ Cells |
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