Paradoxical effects of ZIM3, a CRISPRi effector, on human induced pluripotent stem-cell-derived cardiomyocyte electrophysiology

We show that zinc finger imprinted 3 (Zim3), when used as Zim3-KRAB-dCas9 effector in interference CRISPR, without any guide RNAs, paradoxically up-regulates key cardiac ion channel genes in human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), responsible for healthy resting membra...

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Veröffentlicht in:	PNAS nexus 2024-03, Vol.3 (3), p.pgae027-pgae027
Hauptverfasser:	Han, Julie L, Heinson, Yuli W, Pozo, Maria R, Li, Weizhen, Entcheva, Emilia
Format:	Artikel
Sprache:	eng
Schlagworte:	Brief Report Chromatin DNA binding proteins Electric properties Gene expression Genes Genetic aspects Genetic transcription Heart Heart cells Muscles Physiological aspects RNA sequencing Stem cells
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container_title	PNAS nexus
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creator	Han, Julie L Heinson, Yuli W Pozo, Maria R Li, Weizhen Entcheva, Emilia
description	We show that zinc finger imprinted 3 (Zim3), when used as Zim3-KRAB-dCas9 effector in interference CRISPR, without any guide RNAs, paradoxically up-regulates key cardiac ion channel genes in human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), responsible for healthy resting membrane potential, repolarization of the action potential, and electrical transmission of signals. These were found to yield expected functional enhancements consistent with a more mature iPSC-CM phenotype, with potentially desirable properties.
doi_str_mv	10.1093/pnasnexus/pgae027
format	Article
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source	DOAJ Directory of Open Access Journals; Access via Oxford University Press (Open Access Collection); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects	Brief Report Chromatin DNA binding proteins Electric properties Gene expression Genes Genetic aspects Genetic transcription Heart Heart cells Muscles Physiological aspects RNA sequencing Stem cells
title	Paradoxical effects of ZIM3, a CRISPRi effector, on human induced pluripotent stem-cell-derived cardiomyocyte electrophysiology
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