Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II
Blocking the interaction between the SARS-CoV-2 spike protein and the human angiotensin-converting enzyme II (hACE2) protein serves as a therapeutic strategy for treating COVID-19. Traditional Chinese medicine (TCM) treatments containing bioactive products could alleviate the symptoms of severe COVI...
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| Veröffentlicht in: | International journal of molecular sciences 2024-03, Vol.25 (5), p.2935 |
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| creator | Lin, Cheng-Han Chang, Ho-Ju Lin, Meng-Wei Yang, Xin-Rui Lee, Che-Hsiung Lin, Chih-Sheng |
| description | Blocking the interaction between the SARS-CoV-2 spike protein and the human angiotensin-converting enzyme II (hACE2) protein serves as a therapeutic strategy for treating COVID-19. Traditional Chinese medicine (TCM) treatments containing bioactive products could alleviate the symptoms of severe COVID-19. However, the emergence of SARS-CoV-2 variants has complicated the process of developing broad-spectrum drugs. As such, the aim of this study was to explore the efficacy of TCM treatments against SARS-CoV-2 variants through targeting the interaction of the viral spike protein with the hACE2 receptor. Antiviral activity was systematically evaluated using a pseudovirus system.
(
) was found to be effective against SARS-CoV-2 infection, as it mediated the interaction between the viral spike protein and the hACE2 protein. Moreover, the active molecules of
were identified and analyzed. Baicalein and baicalin, a flavone and a flavone glycoside found in
, respectively, exhibited strong inhibitory activities targeting the viral spike protein and the hACE2 protein, respectively. Under optimized conditions, virus infection was inhibited by 98% via baicalein-treated pseudovirus and baicalin-treated hACE2. In summary, we identified the potential SARS-CoV-2 inhibitors from
that mediate the interaction between the Omicron spike protein and the hACE2 receptor. Future studies on the therapeutic application of baicalein and baicalin against SARS-CoV-2 variants are needed. |
| doi_str_mv | 10.3390/ijms25052935 |
| format | Article |
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(
) was found to be effective against SARS-CoV-2 infection, as it mediated the interaction between the viral spike protein and the hACE2 protein. Moreover, the active molecules of
were identified and analyzed. Baicalein and baicalin, a flavone and a flavone glycoside found in
, respectively, exhibited strong inhibitory activities targeting the viral spike protein and the hACE2 protein, respectively. Under optimized conditions, virus infection was inhibited by 98% via baicalein-treated pseudovirus and baicalin-treated hACE2. In summary, we identified the potential SARS-CoV-2 inhibitors from
that mediate the interaction between the Omicron spike protein and the hACE2 receptor. Future studies on the therapeutic application of baicalein and baicalin against SARS-CoV-2 variants are needed.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25052935</identifier><identifier>PMID: 38474182</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Chinese medicine ; COVID-19 ; Enzymes ; Infections ; Pandemics ; Protein expression ; Proteins ; Severe acute respiratory syndrome coronavirus 2</subject><ispartof>International journal of molecular sciences, 2024-03, Vol.25 (5), p.2935</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-391ebdb849d11b34789cd9f837e13e3cafd104fe13ab5155c9d9c839f00c71b83</citedby><cites>FETCH-LOGICAL-c413t-391ebdb849d11b34789cd9f837e13e3cafd104fe13ab5155c9d9c839f00c71b83</cites><orcidid>0000-0002-6164-4884 ; 0000-0003-1140-7933</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10932139/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10932139/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38474182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Cheng-Han</creatorcontrib><creatorcontrib>Chang, Ho-Ju</creatorcontrib><creatorcontrib>Lin, Meng-Wei</creatorcontrib><creatorcontrib>Yang, Xin-Rui</creatorcontrib><creatorcontrib>Lee, Che-Hsiung</creatorcontrib><creatorcontrib>Lin, Chih-Sheng</creatorcontrib><title>Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Blocking the interaction between the SARS-CoV-2 spike protein and the human angiotensin-converting enzyme II (hACE2) protein serves as a therapeutic strategy for treating COVID-19. Traditional Chinese medicine (TCM) treatments containing bioactive products could alleviate the symptoms of severe COVID-19. However, the emergence of SARS-CoV-2 variants has complicated the process of developing broad-spectrum drugs. As such, the aim of this study was to explore the efficacy of TCM treatments against SARS-CoV-2 variants through targeting the interaction of the viral spike protein with the hACE2 receptor. Antiviral activity was systematically evaluated using a pseudovirus system.
(
) was found to be effective against SARS-CoV-2 infection, as it mediated the interaction between the viral spike protein and the hACE2 protein. Moreover, the active molecules of
were identified and analyzed. Baicalein and baicalin, a flavone and a flavone glycoside found in
, respectively, exhibited strong inhibitory activities targeting the viral spike protein and the hACE2 protein, respectively. Under optimized conditions, virus infection was inhibited by 98% via baicalein-treated pseudovirus and baicalin-treated hACE2. In summary, we identified the potential SARS-CoV-2 inhibitors from
that mediate the interaction between the Omicron spike protein and the hACE2 receptor. Future studies on the therapeutic application of baicalein and baicalin against SARS-CoV-2 variants are needed.</description><subject>Chinese medicine</subject><subject>COVID-19</subject><subject>Enzymes</subject><subject>Infections</subject><subject>Pandemics</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU9v0zAYhy0EYmNw44wsceFANv9JmviEqqqwSkNDFLhGtvO6dUnsznaGytfhi87RxlTwxX79Pnrkn1-EXlNyzrkgF3Y3RFaRiglePUGntGSsIGRWPz06n6AXMe4IYZxV4jk64U1Zl7Rhp-jPym2tssmHA14aY7XUB-wN_iytwws_7L0Dl-J0tdZjgr6XwUqsZCZ7cNHmlsNpC3jlEgSpk821gvQLwOH13v4E_CX4BFk3OeZf18XC_ygYlq7Dl-MgHZ67jc1Elrncc7cQknUbvHS_D0P2rl6iZ0b2EV497Gfo-8flt8VlcXX9abWYXxW6pDwVXFBQnWpK0VGqeFk3QnfCNLwGyoFraTpKSpMLqSpaVVp0QjdcGEJ0TVXDz9CHe-9-VAN0OgcPsm_3wQ4yHFovbftvx9ltu_G3LSWCM8pFNrx7MAR_M0JM7WCjnj7NgR9jy0Q1mzUlEzSjb_9Dd34MLuebqLxqUdWZen9P6eBjDGAeX0NJO42_PR5_xt8cJ3iE_86b3wE5Iq31</recordid><startdate>20240302</startdate><enddate>20240302</enddate><creator>Lin, Cheng-Han</creator><creator>Chang, Ho-Ju</creator><creator>Lin, Meng-Wei</creator><creator>Yang, Xin-Rui</creator><creator>Lee, Che-Hsiung</creator><creator>Lin, Chih-Sheng</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6164-4884</orcidid><orcidid>https://orcid.org/0000-0003-1140-7933</orcidid></search><sort><creationdate>20240302</creationdate><title>Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II</title><author>Lin, Cheng-Han ; Chang, Ho-Ju ; Lin, Meng-Wei ; Yang, Xin-Rui ; Lee, Che-Hsiung ; Lin, Chih-Sheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-391ebdb849d11b34789cd9f837e13e3cafd104fe13ab5155c9d9c839f00c71b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Chinese medicine</topic><topic>COVID-19</topic><topic>Enzymes</topic><topic>Infections</topic><topic>Pandemics</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Cheng-Han</creatorcontrib><creatorcontrib>Chang, Ho-Ju</creatorcontrib><creatorcontrib>Lin, Meng-Wei</creatorcontrib><creatorcontrib>Yang, Xin-Rui</creatorcontrib><creatorcontrib>Lee, Che-Hsiung</creatorcontrib><creatorcontrib>Lin, Chih-Sheng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Cheng-Han</au><au>Chang, Ho-Ju</au><au>Lin, Meng-Wei</au><au>Yang, Xin-Rui</au><au>Lee, Che-Hsiung</au><au>Lin, Chih-Sheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2024-03-02</date><risdate>2024</risdate><volume>25</volume><issue>5</issue><spage>2935</spage><pages>2935-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Blocking the interaction between the SARS-CoV-2 spike protein and the human angiotensin-converting enzyme II (hACE2) protein serves as a therapeutic strategy for treating COVID-19. Traditional Chinese medicine (TCM) treatments containing bioactive products could alleviate the symptoms of severe COVID-19. However, the emergence of SARS-CoV-2 variants has complicated the process of developing broad-spectrum drugs. As such, the aim of this study was to explore the efficacy of TCM treatments against SARS-CoV-2 variants through targeting the interaction of the viral spike protein with the hACE2 receptor. Antiviral activity was systematically evaluated using a pseudovirus system.
(
) was found to be effective against SARS-CoV-2 infection, as it mediated the interaction between the viral spike protein and the hACE2 protein. Moreover, the active molecules of
were identified and analyzed. Baicalein and baicalin, a flavone and a flavone glycoside found in
, respectively, exhibited strong inhibitory activities targeting the viral spike protein and the hACE2 protein, respectively. Under optimized conditions, virus infection was inhibited by 98% via baicalein-treated pseudovirus and baicalin-treated hACE2. In summary, we identified the potential SARS-CoV-2 inhibitors from
that mediate the interaction between the Omicron spike protein and the hACE2 receptor. Future studies on the therapeutic application of baicalein and baicalin against SARS-CoV-2 variants are needed.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38474182</pmid><doi>10.3390/ijms25052935</doi><orcidid>https://orcid.org/0000-0002-6164-4884</orcidid><orcidid>https://orcid.org/0000-0003-1140-7933</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Chinese medicine COVID-19 Enzymes Infections Pandemics Protein expression Proteins Severe acute respiratory syndrome coronavirus 2 |
| title | Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II |
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