Diurnal Oscillation of Amylolytic Activity in Spinach Chloroplasts
Chloroplasts isolated from spinach (Spinacia oleracea L., cv. $\text{vital}^{\text{R}}$) plants grown under controlled light/dark and temperature regimes, contained the phosphorolytic and amylolytic pathways for starch breakdown. The latter consists at least of α-and β-amylase and maltase. Only low...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1978-11, Vol.62 (5), p.687-689 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Pongratz, P Beck, E |
| description | Chloroplasts isolated from spinach (Spinacia oleracea L., cv. $\text{vital}^{\text{R}}$) plants grown under controlled light/dark and temperature regimes, contained the phosphorolytic and amylolytic pathways for starch breakdown. The latter consists at least of α-and β-amylase and maltase. Only low amylolytic activity was observed in chloroplasts isolated during the light phase. In chloroplasts prepared during the dark phase, this activity was almost twice as high. These diurnal oscillations of the amylolytic activity were maintained when the plants were kept in prolonged darkness or continuous light. The amylolytic system exhibited a sharp pH optimum between 5.8 and 6.0. Phosphorylase activity, when assayed with saturating concentrations of inorganic phosphate, did not show diurnal fluctuations. |
| doi_str_mv | 10.1104/pp.62.5.687 |
| format | Article |
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The latter consists at least of α-and β-amylase and maltase. Only low amylolytic activity was observed in chloroplasts isolated during the light phase. In chloroplasts prepared during the dark phase, this activity was almost twice as high. These diurnal oscillations of the amylolytic activity were maintained when the plants were kept in prolonged darkness or continuous light. The amylolytic system exhibited a sharp pH optimum between 5.8 and 6.0. Phosphorylase activity, when assayed with saturating concentrations of inorganic phosphate, did not show diurnal fluctuations.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.62.5.687</identifier><identifier>PMID: 16660584</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>Chloroplasts ; Dextrins ; Enzymes ; Hydrolysis ; Paper chromatography ; Phosphates ; Physiological regulation ; Plants ; Spinach ; Starches</subject><ispartof>Plant physiology (Bethesda), 1978-11, Vol.62 (5), p.687-689</ispartof><rights>Copyright 1978 The American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-cabebea15984350d37b09f08400643e986e988f651d8b36970f09a0c1daa0aab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4265507$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4265507$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16660584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pongratz, P</creatorcontrib><creatorcontrib>Beck, E</creatorcontrib><title>Diurnal Oscillation of Amylolytic Activity in Spinach Chloroplasts</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Chloroplasts isolated from spinach (Spinacia oleracea L., cv. $\text{vital}^{\text{R}}$) plants grown under controlled light/dark and temperature regimes, contained the phosphorolytic and amylolytic pathways for starch breakdown. The latter consists at least of α-and β-amylase and maltase. Only low amylolytic activity was observed in chloroplasts isolated during the light phase. In chloroplasts prepared during the dark phase, this activity was almost twice as high. These diurnal oscillations of the amylolytic activity were maintained when the plants were kept in prolonged darkness or continuous light. The amylolytic system exhibited a sharp pH optimum between 5.8 and 6.0. Phosphorylase activity, when assayed with saturating concentrations of inorganic phosphate, did not show diurnal fluctuations.</description><subject>Chloroplasts</subject><subject>Dextrins</subject><subject>Enzymes</subject><subject>Hydrolysis</subject><subject>Paper chromatography</subject><subject>Phosphates</subject><subject>Physiological regulation</subject><subject>Plants</subject><subject>Spinach</subject><subject>Starches</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><recordid>eNpVkE1rGzEURUVoiF2nq2xDmF0Wwe7T6GOkTcFx2qZg8CLtWmhkTSwjjyaSHPC_j4pNmi4eenAPV9JB6ArDDGOgX4dhxusZm3HRnKExZqSe1oyKT2gMUHYQQo7Q55S2AIAJphdohDnnwAQdo_sHt4-99tUqGee9zi70Veiq-e7ggz9kZ6q5ye7V5UPl-uppcL02m2qx8SGGweuU0yU677RP9svpnKA_P77_XjxOl6ufvxbz5dQQzvPU6Na2VmMmBSUM1qRpQXYgKACnxErBy4iOM7wWLeGygQ6kBoPXWoPWLZmgb8feYd_u7NrYPkft1RDdTseDCtqp_5PebdRzeFUYZI2lLAW3p4IYXvY2ZbVzydjy696GfVINIbTIKgYn6O5ImhhSirZ7vwWD-itdDYPitWKqSC_0zceH_WNPlgtwfQS2KYf4ntOaMwYNeQPUs4fC</recordid><startdate>19781101</startdate><enddate>19781101</enddate><creator>Pongratz, P</creator><creator>Beck, E</creator><general>American Society of Plant Physiologists</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19781101</creationdate><title>Diurnal Oscillation of Amylolytic Activity in Spinach Chloroplasts</title><author>Pongratz, P ; Beck, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-cabebea15984350d37b09f08400643e986e988f651d8b36970f09a0c1daa0aab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Chloroplasts</topic><topic>Dextrins</topic><topic>Enzymes</topic><topic>Hydrolysis</topic><topic>Paper chromatography</topic><topic>Phosphates</topic><topic>Physiological regulation</topic><topic>Plants</topic><topic>Spinach</topic><topic>Starches</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pongratz, P</creatorcontrib><creatorcontrib>Beck, E</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pongratz, P</au><au>Beck, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diurnal Oscillation of Amylolytic Activity in Spinach Chloroplasts</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1978-11-01</date><risdate>1978</risdate><volume>62</volume><issue>5</issue><spage>687</spage><epage>689</epage><pages>687-689</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Chloroplasts isolated from spinach (Spinacia oleracea L., cv. $\text{vital}^{\text{R}}$) plants grown under controlled light/dark and temperature regimes, contained the phosphorolytic and amylolytic pathways for starch breakdown. The latter consists at least of α-and β-amylase and maltase. Only low amylolytic activity was observed in chloroplasts isolated during the light phase. In chloroplasts prepared during the dark phase, this activity was almost twice as high. These diurnal oscillations of the amylolytic activity were maintained when the plants were kept in prolonged darkness or continuous light. The amylolytic system exhibited a sharp pH optimum between 5.8 and 6.0. Phosphorylase activity, when assayed with saturating concentrations of inorganic phosphate, did not show diurnal fluctuations.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>16660584</pmid><doi>10.1104/pp.62.5.687</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1978-11, Vol.62 (5), p.687-689 |
| issn | 0032-0889 1532-2548 |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | Chloroplasts Dextrins Enzymes Hydrolysis Paper chromatography Phosphates Physiological regulation Plants Spinach Starches |
| title | Diurnal Oscillation of Amylolytic Activity in Spinach Chloroplasts |
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