Genotype diversity and antibiotic resistance risk in Aeromonas hydrophila in Sichuan, China
Sichuan is a significant aquaculture province in China, with a total aquaculture output of 1.72 × 10 6 tons in 2022. One of the most significant microorganisms hurting the Sichuan aquaculture is Aeromonas hydrophila , whose genotype and antibiotic resistance are yet unknown. This study isolated a to...
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| Veröffentlicht in: | Brazilian journal of microbiology 2024-03, Vol.55 (1), p.901-910 |
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| container_title | Brazilian journal of microbiology |
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| creator | Peng, Kun Chen, Mengzhu Wang, Yilin Tian, Ziqi Deng, Longjun Li, Tiancai Feng, Yang Ouyang, Ping Huang, Xiaoli Chen, Defang Geng, Yi |
| description | Sichuan is a significant aquaculture province in China, with a total aquaculture output of 1.72 × 10
6
tons in 2022. One of the most significant microorganisms hurting the Sichuan aquaculture is
Aeromonas hydrophila
, whose genotype and antibiotic resistance are yet unknown. This study isolated a total of 64 strains of
A. hydrophila
from various regions during September 2019 to June 2021 within Sichuan province, China. The technique of Multi-Locus Sequence Typing (MLST) was used for the purpose of molecular typing. Meanwhile, identification of antibiotic resistance phenotype and antibiotic resistance gene was performed. The findings of the study revealed that 64 isolates exhibited 29 sequence types (ST) throughout different regions in Sichuan, with 25 of these ST types being newly identified. Notably, the ST251 emerged as the predominant sequence type responsible for the pandemic. The resistance rate of isolated strains to roxithromycin was as high as 98.3%, followed by co-trimoxazole (87.5%), sulfafurazole (87.5%),
imipenem
(80%), amoxicillin (60%), and clindamycin (57.8%). Fifteen strains of
A. hydrophila
exhibited resistance to medicines across a minimum of three categories, suggesting the development of multidrug resistance in these isolates. A total of 63 ARGs were detected from the isolates, which mediated a range of antibiotic resistance mechanisms, with deactivation and efflux potentially serving as the primary mechanisms of antibiotic resistance. This study revealed the diversity of
A. hydrophila
genotypes and the risk of antibiotic resistance in Sichuan, providing reference for scientific and effective control of
A. hydrophila
infection. |
| doi_str_mv | 10.1007/s42770-023-01187-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10920602</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2942204102</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-8f776baa55c3570daabebc31382e70a3b20740bf41795bb970eda92f712bdf393</originalsourceid><addsrcrecordid>eNp9kUtv3CAUhVGVqkmm_QNdRJa6ySJuLw8PZlVFozaNFKmLtqsu0AXjmMQDU7Ajzb8Pk0nTxyJICMT9ONzDIeQthfcUQH7IgkkJNTBeA6WtrNULckSXsq2FgOag7Bsq65a37JAc53wDwBoQ7BU55FKVQekR-XnhQpy2G1d1_s6l7KdthaErc_LGx8nbKrns84TBuir5fFv5UJ27FNcxYK6GbZfiZvAj7s6_eTvMGM6q1eADviYvexyze_O4LsiPz5--r77UV18vLlfnV7UtrU1120u5NIhNY3kjoUM0zlhOS9FJQG4YSAGmF1SqxhglwXWoWC8pM13PFV-Qj3vdzWzWrrMuTAlHvUl-jWmrI3r9byX4QV_HO01BMViW_1uQ00eFFH_NLk967bN144jBxTlr1ireCgBFC_ruP_QmzikUf5opwRgI-iDI9pRNMefk-qduKOhdeHofni6sfghP73yc_O3j6crvtArA90AupXDt0p-3n5G9BwVqplY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2942204102</pqid></control><display><type>article</type><title>Genotype diversity and antibiotic resistance risk in Aeromonas hydrophila in Sichuan, China</title><source>MEDLINE</source><source>SpringerNature Journals</source><source>PubMed Central</source><creator>Peng, Kun ; Chen, Mengzhu ; Wang, Yilin ; Tian, Ziqi ; Deng, Longjun ; Li, Tiancai ; Feng, Yang ; Ouyang, Ping ; Huang, Xiaoli ; Chen, Defang ; Geng, Yi</creator><creatorcontrib>Peng, Kun ; Chen, Mengzhu ; Wang, Yilin ; Tian, Ziqi ; Deng, Longjun ; Li, Tiancai ; Feng, Yang ; Ouyang, Ping ; Huang, Xiaoli ; Chen, Defang ; Geng, Yi</creatorcontrib><description>Sichuan is a significant aquaculture province in China, with a total aquaculture output of 1.72 × 10
6
tons in 2022. One of the most significant microorganisms hurting the Sichuan aquaculture is
Aeromonas hydrophila
, whose genotype and antibiotic resistance are yet unknown. This study isolated a total of 64 strains of
A. hydrophila
from various regions during September 2019 to June 2021 within Sichuan province, China. The technique of Multi-Locus Sequence Typing (MLST) was used for the purpose of molecular typing. Meanwhile, identification of antibiotic resistance phenotype and antibiotic resistance gene was performed. The findings of the study revealed that 64 isolates exhibited 29 sequence types (ST) throughout different regions in Sichuan, with 25 of these ST types being newly identified. Notably, the ST251 emerged as the predominant sequence type responsible for the pandemic. The resistance rate of isolated strains to roxithromycin was as high as 98.3%, followed by co-trimoxazole (87.5%), sulfafurazole (87.5%),
imipenem
(80%), amoxicillin (60%), and clindamycin (57.8%). Fifteen strains of
A. hydrophila
exhibited resistance to medicines across a minimum of three categories, suggesting the development of multidrug resistance in these isolates. A total of 63 ARGs were detected from the isolates, which mediated a range of antibiotic resistance mechanisms, with deactivation and efflux potentially serving as the primary mechanisms of antibiotic resistance. This study revealed the diversity of
A. hydrophila
genotypes and the risk of antibiotic resistance in Sichuan, providing reference for scientific and effective control of
A. hydrophila
infection.</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-023-01187-9</identifier><identifier>PMID: 37999911</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Aeromonas - genetics ; Aeromonas hydrophila ; Aeromonas hydrophila - genetics ; Amoxicillin ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; Aquaculture ; Biomedical and Life Sciences ; China ; Clindamycin ; Cotrimoxazole ; Drug resistance ; Drug Resistance, Microbial ; Efflux ; Food Microbiology ; Genotype ; Genotypes ; Imipenem ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Microorganisms ; Multidrug resistance ; Multilocus Sequence Typing ; Mycology ; Phenotype ; Phenotypes ; Roxithromycin ; Strains (organisms) ; Sulfisoxazole ; Veterinary Microbiology - Research Paper</subject><ispartof>Brazilian journal of microbiology, 2024-03, Vol.55 (1), p.901-910</ispartof><rights>The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c382t-8f776baa55c3570daabebc31382e70a3b20740bf41795bb970eda92f712bdf393</cites><orcidid>0000-0001-7833-6303</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10920602/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10920602/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37999911$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Kun</creatorcontrib><creatorcontrib>Chen, Mengzhu</creatorcontrib><creatorcontrib>Wang, Yilin</creatorcontrib><creatorcontrib>Tian, Ziqi</creatorcontrib><creatorcontrib>Deng, Longjun</creatorcontrib><creatorcontrib>Li, Tiancai</creatorcontrib><creatorcontrib>Feng, Yang</creatorcontrib><creatorcontrib>Ouyang, Ping</creatorcontrib><creatorcontrib>Huang, Xiaoli</creatorcontrib><creatorcontrib>Chen, Defang</creatorcontrib><creatorcontrib>Geng, Yi</creatorcontrib><title>Genotype diversity and antibiotic resistance risk in Aeromonas hydrophila in Sichuan, China</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Sichuan is a significant aquaculture province in China, with a total aquaculture output of 1.72 × 10
6
tons in 2022. One of the most significant microorganisms hurting the Sichuan aquaculture is
Aeromonas hydrophila
, whose genotype and antibiotic resistance are yet unknown. This study isolated a total of 64 strains of
A. hydrophila
from various regions during September 2019 to June 2021 within Sichuan province, China. The technique of Multi-Locus Sequence Typing (MLST) was used for the purpose of molecular typing. Meanwhile, identification of antibiotic resistance phenotype and antibiotic resistance gene was performed. The findings of the study revealed that 64 isolates exhibited 29 sequence types (ST) throughout different regions in Sichuan, with 25 of these ST types being newly identified. Notably, the ST251 emerged as the predominant sequence type responsible for the pandemic. The resistance rate of isolated strains to roxithromycin was as high as 98.3%, followed by co-trimoxazole (87.5%), sulfafurazole (87.5%),
imipenem
(80%), amoxicillin (60%), and clindamycin (57.8%). Fifteen strains of
A. hydrophila
exhibited resistance to medicines across a minimum of three categories, suggesting the development of multidrug resistance in these isolates. A total of 63 ARGs were detected from the isolates, which mediated a range of antibiotic resistance mechanisms, with deactivation and efflux potentially serving as the primary mechanisms of antibiotic resistance. This study revealed the diversity of
A. hydrophila
genotypes and the risk of antibiotic resistance in Sichuan, providing reference for scientific and effective control of
A. hydrophila
infection.</description><subject>Aeromonas - genetics</subject><subject>Aeromonas hydrophila</subject><subject>Aeromonas hydrophila - genetics</subject><subject>Amoxicillin</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Aquaculture</subject><subject>Biomedical and Life Sciences</subject><subject>China</subject><subject>Clindamycin</subject><subject>Cotrimoxazole</subject><subject>Drug resistance</subject><subject>Drug Resistance, Microbial</subject><subject>Efflux</subject><subject>Food Microbiology</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Imipenem</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Multidrug resistance</subject><subject>Multilocus Sequence Typing</subject><subject>Mycology</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Roxithromycin</subject><subject>Strains (organisms)</subject><subject>Sulfisoxazole</subject><subject>Veterinary Microbiology - Research Paper</subject><issn>1517-8382</issn><issn>1678-4405</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv3CAUhVGVqkmm_QNdRJa6ySJuLw8PZlVFozaNFKmLtqsu0AXjmMQDU7Ajzb8Pk0nTxyJICMT9ONzDIeQthfcUQH7IgkkJNTBeA6WtrNULckSXsq2FgOag7Bsq65a37JAc53wDwBoQ7BU55FKVQekR-XnhQpy2G1d1_s6l7KdthaErc_LGx8nbKrns84TBuir5fFv5UJ27FNcxYK6GbZfiZvAj7s6_eTvMGM6q1eADviYvexyze_O4LsiPz5--r77UV18vLlfnV7UtrU1120u5NIhNY3kjoUM0zlhOS9FJQG4YSAGmF1SqxhglwXWoWC8pM13PFV-Qj3vdzWzWrrMuTAlHvUl-jWmrI3r9byX4QV_HO01BMViW_1uQ00eFFH_NLk967bN144jBxTlr1ireCgBFC_ruP_QmzikUf5opwRgI-iDI9pRNMefk-qduKOhdeHofni6sfghP73yc_O3j6crvtArA90AupXDt0p-3n5G9BwVqplY</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Peng, Kun</creator><creator>Chen, Mengzhu</creator><creator>Wang, Yilin</creator><creator>Tian, Ziqi</creator><creator>Deng, Longjun</creator><creator>Li, Tiancai</creator><creator>Feng, Yang</creator><creator>Ouyang, Ping</creator><creator>Huang, Xiaoli</creator><creator>Chen, Defang</creator><creator>Geng, Yi</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7833-6303</orcidid></search><sort><creationdate>20240301</creationdate><title>Genotype diversity and antibiotic resistance risk in Aeromonas hydrophila in Sichuan, China</title><author>Peng, Kun ; 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6
tons in 2022. One of the most significant microorganisms hurting the Sichuan aquaculture is
Aeromonas hydrophila
, whose genotype and antibiotic resistance are yet unknown. This study isolated a total of 64 strains of
A. hydrophila
from various regions during September 2019 to June 2021 within Sichuan province, China. The technique of Multi-Locus Sequence Typing (MLST) was used for the purpose of molecular typing. Meanwhile, identification of antibiotic resistance phenotype and antibiotic resistance gene was performed. The findings of the study revealed that 64 isolates exhibited 29 sequence types (ST) throughout different regions in Sichuan, with 25 of these ST types being newly identified. Notably, the ST251 emerged as the predominant sequence type responsible for the pandemic. The resistance rate of isolated strains to roxithromycin was as high as 98.3%, followed by co-trimoxazole (87.5%), sulfafurazole (87.5%),
imipenem
(80%), amoxicillin (60%), and clindamycin (57.8%). Fifteen strains of
A. hydrophila
exhibited resistance to medicines across a minimum of three categories, suggesting the development of multidrug resistance in these isolates. A total of 63 ARGs were detected from the isolates, which mediated a range of antibiotic resistance mechanisms, with deactivation and efflux potentially serving as the primary mechanisms of antibiotic resistance. This study revealed the diversity of
A. hydrophila
genotypes and the risk of antibiotic resistance in Sichuan, providing reference for scientific and effective control of
A. hydrophila
infection.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>37999911</pmid><doi>10.1007/s42770-023-01187-9</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7833-6303</orcidid></addata></record> |
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| subjects | Aeromonas - genetics Aeromonas hydrophila Aeromonas hydrophila - genetics Amoxicillin Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Aquaculture Biomedical and Life Sciences China Clindamycin Cotrimoxazole Drug resistance Drug Resistance, Microbial Efflux Food Microbiology Genotype Genotypes Imipenem Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Microorganisms Multidrug resistance Multilocus Sequence Typing Mycology Phenotype Phenotypes Roxithromycin Strains (organisms) Sulfisoxazole Veterinary Microbiology - Research Paper |
| title | Genotype diversity and antibiotic resistance risk in Aeromonas hydrophila in Sichuan, China |
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