Transcriptome sequencing of circular RNA reveals the involvement of hsa‐SCMH1_0001 in the pathogenesis of Parkinson's disease
Background Parkinson's disease (PD) is the second most common neurodegenerative disease. Exosomes are endosome‐derived extracellular vesicles that can take part in intercellular communication. Circular RNAs (circRNAs) are noncoding RNAs characterized by covalently closed‐loop structures, which...
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creator | Wang, Qiao Wang, Huizhi Zhao, Xuemin Han, Chunlei Liu, Chong Li, Zhibao Du, Tingting Sui, Yunpeng Zhang, Xin Zhang, Jianguo Xiao, Yilei Cai, Guoen Meng, Fangang |
description | Background
Parkinson's disease (PD) is the second most common neurodegenerative disease. Exosomes are endosome‐derived extracellular vesicles that can take part in intercellular communication. Circular RNAs (circRNAs) are noncoding RNAs characterized by covalently closed‐loop structures, which perform a crucial function in many diseases.
Aim
To clarify the expression and function of exosomal circRNSs of PD patients and look for circRNAs that might be related to the pathogenesis of PD.
Materials and Methods
We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next‐generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction. Correlation analysis was performed between the expression levels of the circRNAs and the clinical characteristics of PD patients. The binding miRNAs and target genes were predicted using TargetScanHuman, miRDB, and miRTarBase. The predicted target genes were compared with the differentially expressed mRNAs in sequencing results.
Results
According to the NGS, 62 upregulated and 37 downregulated circRNAs in the PD group were screened out. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. We identified 17 potential binding miRNAs of hsa‐SCMH1_0001 with 149 potential target genes. ARID1A and C1orf115 belong to the intersection of the predicted target genes and the differentially expressed mRNAs obtained by sequencing.
Conclusion
This study suggested that hsa‐SCMH1_0001 and its target genes ARID1A and C1orf115 are downregulated in PD patients and may be involved in the occurrence of PD.
CircRNA expression profiles were examined in peripheral exosomes from PD patients and healthy controls using next‐generation sequencing. Ninety‐nine DEcircRNAs were screened out and three of them were selected for quantitative polymerase chain reaction. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. Seventeen binding miRNAs of hsa‐SCMH1_0001 and 149 target genes including ARID1A and C1orf115 were predicted using multiple databases. |
doi_str_mv | 10.1111/cns.14435 |
format | Article |
fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10916443</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A800054949</galeid><sourcerecordid>A800054949</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4715-acfb09c9c0c2ab98a272624b8c983f8a0e5f854b27c5a2b237693ef83d2746293</originalsourceid><addsrcrecordid>eNp1ks1u1DAQxyMEoqVw4AVQJA7AYbe2Yyf2Ca1WQCuVgmg5W453suuS2IudLOoJHoFn5Ek6u2lXFAn7YMvzm_98eLLsOSVTiuvY-jSlnBfiQXZIKyEmQnH1cH8vyEH2JKUrQkomlXycHRRVWXIpxWH28zIan2x06z50kCf4PoC3zi_z0OTWRTu0JuZfzmd5hA2YNuX9CnLnN6HdQAe-33KrZP78-n0x_3hCNSGEon2HrU2_CkvwkFzacp9N_OZ8Cv5VyhcugUnwNHvUoCo8uz2Psq_v313OTyZnnz6czmdnE8srKibGNjVRVllimamVNKxiJeO1tEoWjTQERCMFr1llhWE1wwJVAY0sFqziJVPFUfZ21F0PdQcLi5lH0-p1dJ2J1zoYp-9bvFvpZdhoShQtsbeo8PpWIQZsUup155KFtjUewpA0kyUpqaLVFn35D3oVhuixPl0QWqiK053gdKSWpgXtfBMwsMW9gM7Z4KFx-D6T2FHB8UPR4c3oYGNIKUKzT58SvR0EjYOgd4OA7Iu_692Tdz-PwPEI_MAo1_9X0vPzi1HyBpkBvkc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3013974143</pqid></control><display><type>article</type><title>Transcriptome sequencing of circular RNA reveals the involvement of hsa‐SCMH1_0001 in the pathogenesis of Parkinson's disease</title><source>Wiley Online Library Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Wang, Qiao ; Wang, Huizhi ; Zhao, Xuemin ; Han, Chunlei ; Liu, Chong ; Li, Zhibao ; Du, Tingting ; Sui, Yunpeng ; Zhang, Xin ; Zhang, Jianguo ; Xiao, Yilei ; Cai, Guoen ; Meng, Fangang</creator><creatorcontrib>Wang, Qiao ; Wang, Huizhi ; Zhao, Xuemin ; Han, Chunlei ; Liu, Chong ; Li, Zhibao ; Du, Tingting ; Sui, Yunpeng ; Zhang, Xin ; Zhang, Jianguo ; Xiao, Yilei ; Cai, Guoen ; Meng, Fangang</creatorcontrib><description>Background
Parkinson's disease (PD) is the second most common neurodegenerative disease. Exosomes are endosome‐derived extracellular vesicles that can take part in intercellular communication. Circular RNAs (circRNAs) are noncoding RNAs characterized by covalently closed‐loop structures, which perform a crucial function in many diseases.
Aim
To clarify the expression and function of exosomal circRNSs of PD patients and look for circRNAs that might be related to the pathogenesis of PD.
Materials and Methods
We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next‐generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction. Correlation analysis was performed between the expression levels of the circRNAs and the clinical characteristics of PD patients. The binding miRNAs and target genes were predicted using TargetScanHuman, miRDB, and miRTarBase. The predicted target genes were compared with the differentially expressed mRNAs in sequencing results.
Results
According to the NGS, 62 upregulated and 37 downregulated circRNAs in the PD group were screened out. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. We identified 17 potential binding miRNAs of hsa‐SCMH1_0001 with 149 potential target genes. ARID1A and C1orf115 belong to the intersection of the predicted target genes and the differentially expressed mRNAs obtained by sequencing.
Conclusion
This study suggested that hsa‐SCMH1_0001 and its target genes ARID1A and C1orf115 are downregulated in PD patients and may be involved in the occurrence of PD.
CircRNA expression profiles were examined in peripheral exosomes from PD patients and healthy controls using next‐generation sequencing. Ninety‐nine DEcircRNAs were screened out and three of them were selected for quantitative polymerase chain reaction. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. Seventeen binding miRNAs of hsa‐SCMH1_0001 and 149 target genes including ARID1A and C1orf115 were predicted using multiple databases.</description><identifier>ISSN: 1755-5930</identifier><identifier>ISSN: 1755-5949</identifier><identifier>EISSN: 1755-5949</identifier><identifier>DOI: 10.1111/cns.14435</identifier><identifier>PMID: 37664885</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Alzheimer's disease ; Analysis ; Antibodies ; Biomarkers ; Brain ; Circular RNA ; Correlation analysis ; Disease ; Diseases ; Down-regulation ; Endosomes ; Ethylenediaminetetraacetic acid ; Exosomes ; Extracellular vesicles ; Gene expression ; Genes ; Health savings accounts ; MicroRNA ; Movement disorders ; Nervous system ; Neurodegenerative diseases ; Next-generation sequencing ; Original ; Parkinson's disease ; Pathogenesis ; Software ; Transcriptomes</subject><ispartof>CNS neuroscience & therapeutics, 2024-03, Vol.30 (3), p.e14435-n/a</ispartof><rights>2023 The Authors. published by John Wiley & Sons Ltd.</rights><rights>2023 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd.</rights><rights>COPYRIGHT 2024 John Wiley & Sons, Inc.</rights><rights>2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4715-acfb09c9c0c2ab98a272624b8c983f8a0e5f854b27c5a2b237693ef83d2746293</cites><orcidid>0000-0002-0009-0574 ; 0000-0002-8577-6443 ; 0000-0001-7780-2480 ; 0000-0002-0180-1534</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10916443/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10916443/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37664885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Qiao</creatorcontrib><creatorcontrib>Wang, Huizhi</creatorcontrib><creatorcontrib>Zhao, Xuemin</creatorcontrib><creatorcontrib>Han, Chunlei</creatorcontrib><creatorcontrib>Liu, Chong</creatorcontrib><creatorcontrib>Li, Zhibao</creatorcontrib><creatorcontrib>Du, Tingting</creatorcontrib><creatorcontrib>Sui, Yunpeng</creatorcontrib><creatorcontrib>Zhang, Xin</creatorcontrib><creatorcontrib>Zhang, Jianguo</creatorcontrib><creatorcontrib>Xiao, Yilei</creatorcontrib><creatorcontrib>Cai, Guoen</creatorcontrib><creatorcontrib>Meng, Fangang</creatorcontrib><title>Transcriptome sequencing of circular RNA reveals the involvement of hsa‐SCMH1_0001 in the pathogenesis of Parkinson's disease</title><title>CNS neuroscience & therapeutics</title><addtitle>CNS Neurosci Ther</addtitle><description>Background
Parkinson's disease (PD) is the second most common neurodegenerative disease. Exosomes are endosome‐derived extracellular vesicles that can take part in intercellular communication. Circular RNAs (circRNAs) are noncoding RNAs characterized by covalently closed‐loop structures, which perform a crucial function in many diseases.
Aim
To clarify the expression and function of exosomal circRNSs of PD patients and look for circRNAs that might be related to the pathogenesis of PD.
Materials and Methods
We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next‐generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction. Correlation analysis was performed between the expression levels of the circRNAs and the clinical characteristics of PD patients. The binding miRNAs and target genes were predicted using TargetScanHuman, miRDB, and miRTarBase. The predicted target genes were compared with the differentially expressed mRNAs in sequencing results.
Results
According to the NGS, 62 upregulated and 37 downregulated circRNAs in the PD group were screened out. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. We identified 17 potential binding miRNAs of hsa‐SCMH1_0001 with 149 potential target genes. ARID1A and C1orf115 belong to the intersection of the predicted target genes and the differentially expressed mRNAs obtained by sequencing.
Conclusion
This study suggested that hsa‐SCMH1_0001 and its target genes ARID1A and C1orf115 are downregulated in PD patients and may be involved in the occurrence of PD.
CircRNA expression profiles were examined in peripheral exosomes from PD patients and healthy controls using next‐generation sequencing. Ninety‐nine DEcircRNAs were screened out and three of them were selected for quantitative polymerase chain reaction. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. Seventeen binding miRNAs of hsa‐SCMH1_0001 and 149 target genes including ARID1A and C1orf115 were predicted using multiple databases.</description><subject>Alzheimer's disease</subject><subject>Analysis</subject><subject>Antibodies</subject><subject>Biomarkers</subject><subject>Brain</subject><subject>Circular RNA</subject><subject>Correlation analysis</subject><subject>Disease</subject><subject>Diseases</subject><subject>Down-regulation</subject><subject>Endosomes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Exosomes</subject><subject>Extracellular vesicles</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Health savings accounts</subject><subject>MicroRNA</subject><subject>Movement disorders</subject><subject>Nervous system</subject><subject>Neurodegenerative diseases</subject><subject>Next-generation sequencing</subject><subject>Original</subject><subject>Parkinson's disease</subject><subject>Pathogenesis</subject><subject>Software</subject><subject>Transcriptomes</subject><issn>1755-5930</issn><issn>1755-5949</issn><issn>1755-5949</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>BENPR</sourceid><recordid>eNp1ks1u1DAQxyMEoqVw4AVQJA7AYbe2Yyf2Ca1WQCuVgmg5W453suuS2IudLOoJHoFn5Ek6u2lXFAn7YMvzm_98eLLsOSVTiuvY-jSlnBfiQXZIKyEmQnH1cH8vyEH2JKUrQkomlXycHRRVWXIpxWH28zIan2x06z50kCf4PoC3zi_z0OTWRTu0JuZfzmd5hA2YNuX9CnLnN6HdQAe-33KrZP78-n0x_3hCNSGEon2HrU2_CkvwkFzacp9N_OZ8Cv5VyhcugUnwNHvUoCo8uz2Psq_v313OTyZnnz6czmdnE8srKibGNjVRVllimamVNKxiJeO1tEoWjTQERCMFr1llhWE1wwJVAY0sFqziJVPFUfZ21F0PdQcLi5lH0-p1dJ2J1zoYp-9bvFvpZdhoShQtsbeo8PpWIQZsUup155KFtjUewpA0kyUpqaLVFn35D3oVhuixPl0QWqiK053gdKSWpgXtfBMwsMW9gM7Z4KFx-D6T2FHB8UPR4c3oYGNIKUKzT58SvR0EjYOgd4OA7Iu_692Tdz-PwPEI_MAo1_9X0vPzi1HyBpkBvkc</recordid><startdate>202403</startdate><enddate>202403</enddate><creator>Wang, Qiao</creator><creator>Wang, Huizhi</creator><creator>Zhao, Xuemin</creator><creator>Han, Chunlei</creator><creator>Liu, Chong</creator><creator>Li, Zhibao</creator><creator>Du, Tingting</creator><creator>Sui, Yunpeng</creator><creator>Zhang, Xin</creator><creator>Zhang, Jianguo</creator><creator>Xiao, Yilei</creator><creator>Cai, Guoen</creator><creator>Meng, Fangang</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0009-0574</orcidid><orcidid>https://orcid.org/0000-0002-8577-6443</orcidid><orcidid>https://orcid.org/0000-0001-7780-2480</orcidid><orcidid>https://orcid.org/0000-0002-0180-1534</orcidid></search><sort><creationdate>202403</creationdate><title>Transcriptome sequencing of circular RNA reveals the involvement of hsa‐SCMH1_0001 in the pathogenesis of Parkinson's disease</title><author>Wang, Qiao ; Wang, Huizhi ; Zhao, Xuemin ; Han, Chunlei ; Liu, Chong ; Li, Zhibao ; Du, Tingting ; Sui, Yunpeng ; Zhang, Xin ; Zhang, Jianguo ; Xiao, Yilei ; Cai, Guoen ; Meng, Fangang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4715-acfb09c9c0c2ab98a272624b8c983f8a0e5f854b27c5a2b237693ef83d2746293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Alzheimer's disease</topic><topic>Analysis</topic><topic>Antibodies</topic><topic>Biomarkers</topic><topic>Brain</topic><topic>Circular RNA</topic><topic>Correlation analysis</topic><topic>Disease</topic><topic>Diseases</topic><topic>Down-regulation</topic><topic>Endosomes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Exosomes</topic><topic>Extracellular vesicles</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Health savings accounts</topic><topic>MicroRNA</topic><topic>Movement disorders</topic><topic>Nervous system</topic><topic>Neurodegenerative diseases</topic><topic>Next-generation sequencing</topic><topic>Original</topic><topic>Parkinson's disease</topic><topic>Pathogenesis</topic><topic>Software</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Qiao</creatorcontrib><creatorcontrib>Wang, Huizhi</creatorcontrib><creatorcontrib>Zhao, Xuemin</creatorcontrib><creatorcontrib>Han, Chunlei</creatorcontrib><creatorcontrib>Liu, Chong</creatorcontrib><creatorcontrib>Li, Zhibao</creatorcontrib><creatorcontrib>Du, Tingting</creatorcontrib><creatorcontrib>Sui, Yunpeng</creatorcontrib><creatorcontrib>Zhang, Xin</creatorcontrib><creatorcontrib>Zhang, Jianguo</creatorcontrib><creatorcontrib>Xiao, Yilei</creatorcontrib><creatorcontrib>Cai, Guoen</creatorcontrib><creatorcontrib>Meng, Fangang</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>CNS neuroscience & therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Qiao</au><au>Wang, Huizhi</au><au>Zhao, Xuemin</au><au>Han, Chunlei</au><au>Liu, Chong</au><au>Li, Zhibao</au><au>Du, Tingting</au><au>Sui, Yunpeng</au><au>Zhang, Xin</au><au>Zhang, Jianguo</au><au>Xiao, Yilei</au><au>Cai, Guoen</au><au>Meng, Fangang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome sequencing of circular RNA reveals the involvement of hsa‐SCMH1_0001 in the pathogenesis of Parkinson's disease</atitle><jtitle>CNS neuroscience & therapeutics</jtitle><addtitle>CNS Neurosci Ther</addtitle><date>2024-03</date><risdate>2024</risdate><volume>30</volume><issue>3</issue><spage>e14435</spage><epage>n/a</epage><pages>e14435-n/a</pages><issn>1755-5930</issn><issn>1755-5949</issn><eissn>1755-5949</eissn><abstract>Background
Parkinson's disease (PD) is the second most common neurodegenerative disease. Exosomes are endosome‐derived extracellular vesicles that can take part in intercellular communication. Circular RNAs (circRNAs) are noncoding RNAs characterized by covalently closed‐loop structures, which perform a crucial function in many diseases.
Aim
To clarify the expression and function of exosomal circRNSs of PD patients and look for circRNAs that might be related to the pathogenesis of PD.
Materials and Methods
We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next‐generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction. Correlation analysis was performed between the expression levels of the circRNAs and the clinical characteristics of PD patients. The binding miRNAs and target genes were predicted using TargetScanHuman, miRDB, and miRTarBase. The predicted target genes were compared with the differentially expressed mRNAs in sequencing results.
Results
According to the NGS, 62 upregulated and 37 downregulated circRNAs in the PD group were screened out. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. We identified 17 potential binding miRNAs of hsa‐SCMH1_0001 with 149 potential target genes. ARID1A and C1orf115 belong to the intersection of the predicted target genes and the differentially expressed mRNAs obtained by sequencing.
Conclusion
This study suggested that hsa‐SCMH1_0001 and its target genes ARID1A and C1orf115 are downregulated in PD patients and may be involved in the occurrence of PD.
CircRNA expression profiles were examined in peripheral exosomes from PD patients and healthy controls using next‐generation sequencing. Ninety‐nine DEcircRNAs were screened out and three of them were selected for quantitative polymerase chain reaction. Correlation analysis revealed that hsa‐SCMH1_0001 has strong clinical relevance. Seventeen binding miRNAs of hsa‐SCMH1_0001 and 149 target genes including ARID1A and C1orf115 were predicted using multiple databases.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>37664885</pmid><doi>10.1111/cns.14435</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-0009-0574</orcidid><orcidid>https://orcid.org/0000-0002-8577-6443</orcidid><orcidid>https://orcid.org/0000-0001-7780-2480</orcidid><orcidid>https://orcid.org/0000-0002-0180-1534</orcidid><oa>free_for_read</oa></addata></record> |
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source | Wiley Online Library Open Access; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Alzheimer's disease Analysis Antibodies Biomarkers Brain Circular RNA Correlation analysis Disease Diseases Down-regulation Endosomes Ethylenediaminetetraacetic acid Exosomes Extracellular vesicles Gene expression Genes Health savings accounts MicroRNA Movement disorders Nervous system Neurodegenerative diseases Next-generation sequencing Original Parkinson's disease Pathogenesis Software Transcriptomes |
title | Transcriptome sequencing of circular RNA reveals the involvement of hsa‐SCMH1_0001 in the pathogenesis of Parkinson's disease |
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