Native collagen VI delays early muscle stem cell differentiation
Adult muscle stem cells (MuSCs) are critical for muscle homeostasis and regeneration, and their behavior relies on a finely regulated niche made of specific extracellular matrix (ECM) components and soluble factors. Among ECM proteins, collagen VI (Col6) influences the mechanical properties of the n...
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| Veröffentlicht in: | Journal of cell science 2024-02, Vol.137 (3) |
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| container_title | Journal of cell science |
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| creator | Metti, Samuele Da Ros, Francesco Toniato, Giorgia Cescon, Matilde Bonaldo, Paolo |
| description | Adult muscle stem cells (MuSCs) are critical for muscle homeostasis and regeneration, and their behavior relies on a finely regulated niche made of specific extracellular matrix (ECM) components and soluble factors. Among ECM proteins, collagen VI (Col6) influences the mechanical properties of the niche and, in turn, MuSC self-renewal capabilities. Here, we investigated whether Col6 can exert a direct function as a biochemical signal for regulating the stemness and differentiation of murine MuSCs and myoblasts. Native Col6, but not its pepsin-resistant fragment, counteracts the early differentiation of myogenic cells by reducing the expression of differentiation marker genes and preserving stemness features, with inhibition of the canonical Wnt pathway. Our data indicate that extracellular Col6 acts as a soluble ligand in delaying early myogenic differentiation by regulating intracellular signals involved in adult myogenesis. |
| doi_str_mv | 10.1242/jcs.261419 |
| format | Article |
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Among ECM proteins, collagen VI (Col6) influences the mechanical properties of the niche and, in turn, MuSC self-renewal capabilities. Here, we investigated whether Col6 can exert a direct function as a biochemical signal for regulating the stemness and differentiation of murine MuSCs and myoblasts. Native Col6, but not its pepsin-resistant fragment, counteracts the early differentiation of myogenic cells by reducing the expression of differentiation marker genes and preserving stemness features, with inhibition of the canonical Wnt pathway. 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Our data indicate that extracellular Col6 acts as a soluble ligand in delaying early myogenic differentiation by regulating intracellular signals involved in adult myogenesis.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Collagen - metabolism</subject><subject>Mice</subject><subject>Muscle Development - genetics</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscles</subject><subject>Satellite Cells, Skeletal Muscle</subject><subject>Short Report</subject><issn>0021-9533</issn><issn>1477-9137</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtOwzAQRS0EoqWw4QNQlggpxa_4sQJU8ahUwQbYWo4zKamcpMRJpf49iVoqWM3Cx3fuHIQuCZ4SyuntyoUpFYQTfYTGhEsZa8LkMRpjTEmsE8ZG6CyEFcZYUi1P0YgpSjlJ6Bjdv9q22EDkau_tEqrocx5l4O02RGAbv43KLjgPUWihjBx4H2VFnkMDVVv0P-vqHJ3k1ge42M8J-nh6fJ-9xIu35_nsYRE7xlkb9_W0EEJJIQm2LAcGeWoFV84CE5wqK3FOlRNYa8EUS3MlNRYAqUsykXI2QXe73HWXlpC5vkBjvVk3RWmbraltYf6_VMWXWdYbQ7AmhKoh4Xqf0NTfHYTWlEUYTrIV1F0wVBNOE4LlgN7sUNfUITSQH_YQbAbnpnduds57-OpvswP6K5n9AFRifNc</recordid><startdate>20240201</startdate><enddate>20240201</enddate><creator>Metti, Samuele</creator><creator>Da Ros, Francesco</creator><creator>Toniato, Giorgia</creator><creator>Cescon, Matilde</creator><creator>Bonaldo, Paolo</creator><general>The Company of Biologists Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9571-8140</orcidid></search><sort><creationdate>20240201</creationdate><title>Native collagen VI delays early muscle stem cell differentiation</title><author>Metti, Samuele ; Da Ros, Francesco ; Toniato, Giorgia ; Cescon, Matilde ; Bonaldo, Paolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-6149666876710a3fe3efba648cae36428a70f28c60996383bf87906eebc5d6b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Collagen - metabolism</topic><topic>Mice</topic><topic>Muscle Development - genetics</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscles</topic><topic>Satellite Cells, Skeletal Muscle</topic><topic>Short Report</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Metti, Samuele</creatorcontrib><creatorcontrib>Da Ros, Francesco</creatorcontrib><creatorcontrib>Toniato, Giorgia</creatorcontrib><creatorcontrib>Cescon, Matilde</creatorcontrib><creatorcontrib>Bonaldo, Paolo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Metti, Samuele</au><au>Da Ros, Francesco</au><au>Toniato, Giorgia</au><au>Cescon, Matilde</au><au>Bonaldo, Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Native collagen VI delays early muscle stem cell differentiation</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>2024-02-01</date><risdate>2024</risdate><volume>137</volume><issue>3</issue><issn>0021-9533</issn><issn>1477-9137</issn><eissn>1477-9137</eissn><abstract>Adult muscle stem cells (MuSCs) are critical for muscle homeostasis and regeneration, and their behavior relies on a finely regulated niche made of specific extracellular matrix (ECM) components and soluble factors. 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Company of Biologists |
| subjects | Animals Cell Differentiation Collagen - metabolism Mice Muscle Development - genetics Muscle, Skeletal - metabolism Muscles Satellite Cells, Skeletal Muscle Short Report |
| title | Native collagen VI delays early muscle stem cell differentiation |
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