Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings
Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography....
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| Veröffentlicht in: | Plant physiology (Bethesda) 1967-04, Vol.42 (4), p.520-524 |
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| container_end_page | 524 |
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| container_issue | 4 |
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| container_title | Plant physiology (Bethesda) |
| container_volume | 42 |
| creator | Rayle, David L. Purves, William K. |
| description | Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thin layer and gas chromatography, and ultraviolet and visible spectroscopy, as well as by physiological characteristics. |
| doi_str_mv | 10.1104/pp.42.4.520 |
| format | Article |
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Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. 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Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thin layer and gas chromatography, and ultraviolet and visible spectroscopy, as well as by physiological characteristics.</description><subject>Cucumbers</subject><subject>Ethyl ethers</subject><subject>Hypocotyls</subject><subject>Indoles</subject><subject>Plant growth</subject><subject>Plants</subject><subject>Reagents</subject><subject>Seedlings</subject><subject>Silica gel</subject><subject>Solvents</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><recordid>eNpVkUFr3DAQhUVpaDabnHotwbemBG9mJNmWL4WwpM3CQg7ZnIUsS1kvsuVIdiH_Pg67bNLTDPM-3gzzCPmOsEAEftP3C04XfJFR-EJmmDGa0oyLr2QGMPUgRHlKzmLcAQAy5N_IKeZ5lk_gjDytondqaHyXqK5OVrXphsY2ej_yNll1tXcmZendsFWdd8nVJrz2g--33v1KbPBtshz12FYmJI_G1K7pnuM5ObHKRXNxqHOy-XO3Wd6n64e_q-XtOtWc0SHVxqCuhC0YWJVbgZAVpdW1sRWIsjAZZlQzjcJWWpQVx9qWAgWnQlALJZuT33vbfqxaU-vp9qCc7EPTqvAqvWrk_0rXbOWz_ycRRJ4V-WTw82AQ_Mto4iDbJmrjnOqMH6MsGOOCcoSJvN6TOvgYg7HHLQjyPQbZ95JTyeUUw0Rffj7sgz38fQJ-7IFdHHw46pzmCEjZG1jFjc8</recordid><startdate>19670401</startdate><enddate>19670401</enddate><creator>Rayle, David L.</creator><creator>Purves, William K.</creator><general>American Society of Plant Physiologists</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19670401</creationdate><title>Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings</title><author>Rayle, David L. ; Purves, William K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-cee1cb8f730fa6f810579fcdefb0897e5152c3c18fbc89b41df981842882f093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>Cucumbers</topic><topic>Ethyl ethers</topic><topic>Hypocotyls</topic><topic>Indoles</topic><topic>Plant growth</topic><topic>Plants</topic><topic>Reagents</topic><topic>Seedlings</topic><topic>Silica gel</topic><topic>Solvents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rayle, David L.</creatorcontrib><creatorcontrib>Purves, William K.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rayle, David L.</au><au>Purves, William K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1967-04-01</date><risdate>1967</risdate><volume>42</volume><issue>4</issue><spage>520</spage><epage>524</epage><pages>520-524</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. 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| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1967-04, Vol.42 (4), p.520-524 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1086576 |
| source | Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Cucumbers Ethyl ethers Hypocotyls Indoles Plant growth Plants Reagents Seedlings Silica gel Solvents |
| title | Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings |
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