An efficient genetic screen in mammalian cultured cells
Genetic approaches in mammalian cultured cells had limited success because the isolation of mutants and the identification of the mutated genes were often difficult. In the present report, we describe the establishment of a novel genetic screen in Cos‐7 cells that allows rapid identification of poly...
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| Veröffentlicht in: | EMBO reports 2002-07, Vol.3 (7), p.682-687 |
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| creator | Schmelzl, Birgit Geli, M Isabel |
| description | Genetic approaches in mammalian cultured cells had limited success because the isolation of mutants and the identification of the mutated genes were often difficult. In the present report, we describe the establishment of a novel genetic screen in Cos‐7 cells that allows rapid identification of polypeptides whose overexpression inhibits a certain cellular process. We demonstrate that this approach can be used successfully to isolate partial cDNAs whose overexpression specifically interfered with the clathrin‐mediated endocytosis of transferrin. |
| doi_str_mv | 10.1093/embo-reports/kvf131 |
| format | Article |
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In the present report, we describe the establishment of a novel genetic screen in Cos‐7 cells that allows rapid identification of polypeptides whose overexpression inhibits a certain cellular process. 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In the present report, we describe the establishment of a novel genetic screen in Cos‐7 cells that allows rapid identification of polypeptides whose overexpression inhibits a certain cellular process. We demonstrate that this approach can be used successfully to isolate partial cDNAs whose overexpression specifically interfered with the clathrin‐mediated endocytosis of transferrin.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Brain Chemistry</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>Clathrin - metabolism</subject><subject>COS Cells</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - metabolism</subject><subject>Endocytosis - physiology</subject><subject>Flow Cytometry</subject><subject>Genes, Reporter</subject><subject>Genetic Testing - methods</subject><subject>Green Fluorescent Proteins</subject><subject>Humans</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>Mammals</subject><subject>Molecular Sequence Data</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Rats</subject><subject>Receptors, Transferrin - genetics</subject><subject>Receptors, Transferrin - metabolism</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Reproducibility of Results</subject><subject>Scientific Report</subject><subject>Scientific Reports</subject><subject>Transferrin - metabolism</subject><issn>1469-221X</issn><issn>1469-3178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkdFr1TAYxYMobk7_AkGKD77VfV_SNsmLsN3NKUwFmSi-hNz06zVbm16Tdrr_3l5aNvVBfEog53dyDoexpwgvEbQ4pG7d55G2fRzS4dV1gwLvsX0sKp0LlOr-cuccv-yxRyldAkCppXrI9pAjIALfZ_IoZNQ03nkKQ7ahQIN3WXKRKGQ-ZJ3tOtt6GzI3tsMYqc4ctW16zB40tk30ZDkP2KfXpxerN_n5h7O3q6Pz3JUVyNySXTcVrBWRUygFkIamwRpFUQoqhOOE0jlRNyRcUQEqoZ2odI0oZS20OGCvZt_tuO6odlPKaFuzjb6z8cb01ps_X4L_Zjb9tUFQBapyMnixGMT--0hpMJ1Puwo2UD8mI1EpzQEm4fO_hJf9GMNUznBQJQeud25iFrnYpxSpuU2CYHarmN0qZlnFzKtM1LPfS9wxywyTQM2CH76lm__xNKfvjj8ilxNazGiaqLCheBf735HyGfNpoJ-3P9p4ZSopZGk-vz8zcHJSHa8uSvNV_AJGocKj</recordid><startdate>200207</startdate><enddate>200207</enddate><creator>Schmelzl, Birgit</creator><creator>Geli, M Isabel</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200207</creationdate><title>An efficient genetic screen in mammalian cultured cells</title><author>Schmelzl, Birgit ; Geli, M Isabel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5607-aeabf60b8eec81730e90ff1d13453e43c2e17cc3dfe3c4601839c369d1177d393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Brain Chemistry</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>Clathrin - 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In the present report, we describe the establishment of a novel genetic screen in Cos‐7 cells that allows rapid identification of polypeptides whose overexpression inhibits a certain cellular process. We demonstrate that this approach can be used successfully to isolate partial cDNAs whose overexpression specifically interfered with the clathrin‐mediated endocytosis of transferrin.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>12101102</pmid><doi>10.1093/embo-reports/kvf131</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; PubMed Central |
| subjects | Amino Acid Sequence Animals Brain Chemistry Cell Separation Cells, Cultured Clathrin - metabolism COS Cells DNA, Complementary - genetics DNA, Complementary - metabolism Endocytosis - physiology Flow Cytometry Genes, Reporter Genetic Testing - methods Green Fluorescent Proteins Humans Luminescent Proteins - genetics Luminescent Proteins - metabolism Mammals Molecular Sequence Data Peptides - genetics Peptides - metabolism Rats Receptors, Transferrin - genetics Receptors, Transferrin - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Reproducibility of Results Scientific Report Scientific Reports Transferrin - metabolism |
| title | An efficient genetic screen in mammalian cultured cells |
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