An inner membrane platform in the type II secretion machinery of Gram-negative bacteria
The type II secretion machinery allows most Gram‐negative bacteria to deliver virulence factors into their surroundings. We report that in Erwinia chrysanthemi , GspE (the putative NTPase), GspF, GspL and GspM constitute a complex in the inner membrane that is presumably used as a platform for assem...
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| Veröffentlicht in: | EMBO reports 2001-03, Vol.2 (3), p.244-248 |
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| creator | Py, Béatrice Loiseau, Laurent Barras, Frédéric |
| description | The type II secretion machinery allows most Gram‐negative bacteria to deliver virulence factors into their surroundings. We report that in
Erwinia chrysanthemi
, GspE (the putative NTPase), GspF, GspL and GspM constitute a complex in the inner membrane that is presumably used as a platform for assembling other parts of the secretion machinery. The GspE–GspF–GspL–GspM complex was demonstrated by two methods: (i) co‐immunoprecipitation of GspE–GspF–GspL with antibodies raised against either GspE or GspF; (ii) interactions in the yeast two‐hybrid system between GspF and GspE, GspF and GspL, GspL and GspM. GspL was found to have an essential role in complex formation. We propose a model in which the GspE–GspF–GspL–GspM proteins constitute a building block within the secretion machinery on top of which another building block, referred to as a pseudopilus, assembles. By analogy, we predict that a similar platform is required for the biogenesis of the type IV pilus. |
| doi_str_mv | 10.1093/embo-reports/kve042 |
| format | Article |
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Erwinia chrysanthemi
, GspE (the putative NTPase), GspF, GspL and GspM constitute a complex in the inner membrane that is presumably used as a platform for assembling other parts of the secretion machinery. The GspE–GspF–GspL–GspM complex was demonstrated by two methods: (i) co‐immunoprecipitation of GspE–GspF–GspL with antibodies raised against either GspE or GspF; (ii) interactions in the yeast two‐hybrid system between GspF and GspE, GspF and GspL, GspL and GspM. GspL was found to have an essential role in complex formation. We propose a model in which the GspE–GspF–GspL–GspM proteins constitute a building block within the secretion machinery on top of which another building block, referred to as a pseudopilus, assembles. By analogy, we predict that a similar platform is required for the biogenesis of the type IV pilus.</description><identifier>ISSN: 1469-221X</identifier><identifier>EISSN: 1469-3178</identifier><identifier>DOI: 10.1093/embo-reports/kve042</identifier><identifier>PMID: 11266368</identifier><identifier>CODEN: ERMEAX</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Bacteria ; Bacterial Outer Membrane Proteins - metabolism ; Carrier Proteins - metabolism ; Cell Membrane - metabolism ; Gram-Negative Bacteria - genetics ; Gram-Negative Bacteria - metabolism ; Gram-Negative Bacteria - pathogenicity ; Life Sciences ; Models, Biological ; Pectobacterium chrysanthemi - genetics ; Pectobacterium chrysanthemi - metabolism ; Pectobacterium chrysanthemi - pathogenicity ; Scientific Report ; Scientific Reports ; Two-Hybrid System Techniques ; Virulence ; Yeasts</subject><ispartof>EMBO reports, 2001-03, Vol.2 (3), p.244-248</ispartof><rights>European Molecular Biology Organization 2001</rights><rights>Copyright © 2001 European Molecular Biology Organization</rights><rights>Copyright Oxford University Press(England) Mar 15, 2001</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2001 European Molecular Biology Organization 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6176-d6341f897e8d732851ac6d66cbd5747f57ea672e5c6ba12c9a101831aaf291ce3</citedby><cites>FETCH-LOGICAL-c6176-d6341f897e8d732851ac6d66cbd5747f57ea672e5c6ba12c9a101831aaf291ce3</cites><orcidid>0000-0002-9541-8084 ; 0000-0003-3458-2574</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1083838/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1083838/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11266368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://amu.hal.science/hal-03616250$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Py, Béatrice</creatorcontrib><creatorcontrib>Loiseau, Laurent</creatorcontrib><creatorcontrib>Barras, Frédéric</creatorcontrib><title>An inner membrane platform in the type II secretion machinery of Gram-negative bacteria</title><title>EMBO reports</title><addtitle>EMBO Rep</addtitle><addtitle>EMBO Rep</addtitle><description>The type II secretion machinery allows most Gram‐negative bacteria to deliver virulence factors into their surroundings. We report that in
Erwinia chrysanthemi
, GspE (the putative NTPase), GspF, GspL and GspM constitute a complex in the inner membrane that is presumably used as a platform for assembling other parts of the secretion machinery. The GspE–GspF–GspL–GspM complex was demonstrated by two methods: (i) co‐immunoprecipitation of GspE–GspF–GspL with antibodies raised against either GspE or GspF; (ii) interactions in the yeast two‐hybrid system between GspF and GspE, GspF and GspL, GspL and GspM. GspL was found to have an essential role in complex formation. We propose a model in which the GspE–GspF–GspL–GspM proteins constitute a building block within the secretion machinery on top of which another building block, referred to as a pseudopilus, assembles. By analogy, we predict that a similar platform is required for the biogenesis of the type IV pilus.</description><subject>Bacteria</subject><subject>Bacterial Outer Membrane Proteins - metabolism</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Gram-Negative Bacteria - genetics</subject><subject>Gram-Negative Bacteria - metabolism</subject><subject>Gram-Negative Bacteria - pathogenicity</subject><subject>Life Sciences</subject><subject>Models, Biological</subject><subject>Pectobacterium chrysanthemi - genetics</subject><subject>Pectobacterium chrysanthemi - metabolism</subject><subject>Pectobacterium chrysanthemi - pathogenicity</subject><subject>Scientific Report</subject><subject>Scientific Reports</subject><subject>Two-Hybrid System Techniques</subject><subject>Virulence</subject><subject>Yeasts</subject><issn>1469-221X</issn><issn>1469-3178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkV9v0zAUxS0EYqPwCZCQxQMSD2F2nNjJC1IZo61W_giBhnixXPem9ZbYwXYL_fa4StXBniY_2Lr-nXPtexB6TskbSmp2Bt3CZR5652M4u9kCKfIH6JQWvM4YFdXDwznP6Y8T9CSEa0JIWYvqMTqhNOec8eoUXY0tNtaCx13y88oC7lsVG-e7VMdxDTjuesCzGQ6gPUTjLO6UXpuk2WHX4IlXXWZhpaLZAl4oHcEb9RQ9alQb4NlhH6HvHy6-nU-z-efJ7Hw8zzSngmdLzgraVLWAailYXpVUab7kXC-WpShEUwpQXORQar5QNNe1ooRWjCrV5DXVwEbo7eDbbxYdLDXY6FUre2865XfSKSP_v7FmLVduKymp2H6N0OvBYH1HNh3P5b5GGKc8L8mWJvbVoZl3vzYQouxM0NC2aWxuE6QQJA2WkAS-vANeu423aRAyJ-mTQnCRIDZA2rsQPDTH9pTIfcJyn7A8JCyHhJPqxb8fvtUcIk1ANQC_TQu7-3jKi4_vvhZJPULFIA1JZVfgb599rydZFTceji33rB98swEwIcKf473yNzLNQZTy6tNETsVlcfn-y09ZsL_nt-bm</recordid><startdate>200103</startdate><enddate>200103</enddate><creator>Py, Béatrice</creator><creator>Loiseau, Laurent</creator><creator>Barras, Frédéric</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>EMBO Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9541-8084</orcidid><orcidid>https://orcid.org/0000-0003-3458-2574</orcidid></search><sort><creationdate>200103</creationdate><title>An inner membrane platform in the type II secretion machinery of Gram-negative bacteria</title><author>Py, Béatrice ; Loiseau, Laurent ; Barras, Frédéric</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6176-d6341f897e8d732851ac6d66cbd5747f57ea672e5c6ba12c9a101831aaf291ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bacteria</topic><topic>Bacterial Outer Membrane Proteins - metabolism</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Gram-Negative Bacteria - genetics</topic><topic>Gram-Negative Bacteria - metabolism</topic><topic>Gram-Negative Bacteria - pathogenicity</topic><topic>Life Sciences</topic><topic>Models, Biological</topic><topic>Pectobacterium chrysanthemi - genetics</topic><topic>Pectobacterium chrysanthemi - metabolism</topic><topic>Pectobacterium chrysanthemi - pathogenicity</topic><topic>Scientific Report</topic><topic>Scientific Reports</topic><topic>Two-Hybrid System Techniques</topic><topic>Virulence</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Py, Béatrice</creatorcontrib><creatorcontrib>Loiseau, Laurent</creatorcontrib><creatorcontrib>Barras, Frédéric</creatorcontrib><collection>Istex</collection><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>EMBO reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Py, Béatrice</au><au>Loiseau, Laurent</au><au>Barras, Frédéric</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An inner membrane platform in the type II secretion machinery of Gram-negative bacteria</atitle><jtitle>EMBO reports</jtitle><stitle>EMBO Rep</stitle><addtitle>EMBO Rep</addtitle><date>2001-03</date><risdate>2001</risdate><volume>2</volume><issue>3</issue><spage>244</spage><epage>248</epage><pages>244-248</pages><issn>1469-221X</issn><eissn>1469-3178</eissn><coden>ERMEAX</coden><abstract>The type II secretion machinery allows most Gram‐negative bacteria to deliver virulence factors into their surroundings. We report that in
Erwinia chrysanthemi
, GspE (the putative NTPase), GspF, GspL and GspM constitute a complex in the inner membrane that is presumably used as a platform for assembling other parts of the secretion machinery. The GspE–GspF–GspL–GspM complex was demonstrated by two methods: (i) co‐immunoprecipitation of GspE–GspF–GspL with antibodies raised against either GspE or GspF; (ii) interactions in the yeast two‐hybrid system between GspF and GspE, GspF and GspL, GspL and GspM. GspL was found to have an essential role in complex formation. We propose a model in which the GspE–GspF–GspL–GspM proteins constitute a building block within the secretion machinery on top of which another building block, referred to as a pseudopilus, assembles. By analogy, we predict that a similar platform is required for the biogenesis of the type IV pilus.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>11266368</pmid><doi>10.1093/embo-reports/kve042</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-9541-8084</orcidid><orcidid>https://orcid.org/0000-0003-3458-2574</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | EMBO reports, 2001-03, Vol.2 (3), p.244-248 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bacteria Bacterial Outer Membrane Proteins - metabolism Carrier Proteins - metabolism Cell Membrane - metabolism Gram-Negative Bacteria - genetics Gram-Negative Bacteria - metabolism Gram-Negative Bacteria - pathogenicity Life Sciences Models, Biological Pectobacterium chrysanthemi - genetics Pectobacterium chrysanthemi - metabolism Pectobacterium chrysanthemi - pathogenicity Scientific Report Scientific Reports Two-Hybrid System Techniques Virulence Yeasts |
| title | An inner membrane platform in the type II secretion machinery of Gram-negative bacteria |
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