Evaluation of the proline-rich antigen of Coccidioides immitis as a vaccine candidate in mice
We have expressed the proline-rich antigen (PRA) from Coccidioides immitis in Escherichia coli and evaluated its potential as a vaccine candidate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the recombinant protein (rPRA) revealed two bands, which exhibited virtually identi...
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| Veröffentlicht in: | Infection and immunity 1998-08, Vol.66 (8), p.3519-3522 |
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| creator | KIRKLAND, T. N FINLEY, F ORSBORN, K. I GALGIANI, J. N |
| description | We have expressed the proline-rich antigen (PRA) from Coccidioides immitis in Escherichia coli and evaluated its potential as a vaccine candidate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the recombinant protein (rPRA) revealed two bands, which exhibited virtually identical primary amino acid sequences. T cells from rPRA-immunized BALB/c mice showed a significant in vitro proliferative response to rPRA. A small but statistically significant proliferative response was also induced by rPRA in T cells from mice immunized with whole-cell coccidioidal vaccines. BALB/c mice immunized with rPRA and challenged intraperitoneally with virulent C. immitis had a greatly reduced fungal burden in their lungs and spleens compared to unvaccinated mice. The number of organisms in the lungs was reduced 500-fold, and similar reductions were observed in the spleens of immunized mice. These studies support the continued development of rPRA as a candidate vaccine for prevention of coccidioidomycosis. |
| doi_str_mv | 10.1128/IAI.66.8.3519-3522.1998 |
| format | Article |
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N ; FINLEY, F ; ORSBORN, K. I ; GALGIANI, J. N</creator><creatorcontrib>KIRKLAND, T. N ; FINLEY, F ; ORSBORN, K. I ; GALGIANI, J. N</creatorcontrib><description>We have expressed the proline-rich antigen (PRA) from Coccidioides immitis in Escherichia coli and evaluated its potential as a vaccine candidate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the recombinant protein (rPRA) revealed two bands, which exhibited virtually identical primary amino acid sequences. T cells from rPRA-immunized BALB/c mice showed a significant in vitro proliferative response to rPRA. A small but statistically significant proliferative response was also induced by rPRA in T cells from mice immunized with whole-cell coccidioidal vaccines. BALB/c mice immunized with rPRA and challenged intraperitoneally with virulent C. immitis had a greatly reduced fungal burden in their lungs and spleens compared to unvaccinated mice. The number of organisms in the lungs was reduced 500-fold, and similar reductions were observed in the spleens of immunized mice. These studies support the continued development of rPRA as a candidate vaccine for prevention of coccidioidomycosis.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.66.8.3519-3522.1998</identifier><identifier>PMID: 9673228</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Antigens, Fungal - genetics ; Antigens, Fungal - immunology ; Bacteriology ; Biological and medical sciences ; Cell Division ; Cells, Cultured ; Coccidioidomycosis - immunology ; Coccidioidomycosis - microbiology ; Coccidioidomycosis - prevention & control ; Coccidiosis ; Disease Models, Animal ; Drug Evaluation ; Female ; Fundamental and applied biological sciences. Psychology ; Fungal and Parasitic Infections ; Fungal Proteins ; Fungal Vaccines - genetics ; Fungal Vaccines - immunology ; Gene Expression ; Glycoproteins - genetics ; Glycoproteins - immunology ; Human protozoal diseases ; Infectious diseases ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Microbiology ; Parasitic diseases ; Proline - immunology ; Protozoal diseases ; T-Lymphocytes - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, Synthetic - immunology</subject><ispartof>Infection and immunity, 1998-08, Vol.66 (8), p.3519-3522</ispartof><rights>1998 INIST-CNRS</rights><rights>Copyright © 1998, American Society for Microbiology 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-fe2a1486c7752fc407d40f7ebd749dd85e8d092fbb273ad09852d9ec9c80549b3</citedby><cites>FETCH-LOGICAL-c471t-fe2a1486c7752fc407d40f7ebd749dd85e8d092fbb273ad09852d9ec9c80549b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC108381/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC108381/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2392198$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9673228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIRKLAND, T. N</creatorcontrib><creatorcontrib>FINLEY, F</creatorcontrib><creatorcontrib>ORSBORN, K. I</creatorcontrib><creatorcontrib>GALGIANI, J. N</creatorcontrib><title>Evaluation of the proline-rich antigen of Coccidioides immitis as a vaccine candidate in mice</title><title>Infection and immunity</title><addtitle>Infect Immun</addtitle><description>We have expressed the proline-rich antigen (PRA) from Coccidioides immitis in Escherichia coli and evaluated its potential as a vaccine candidate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the recombinant protein (rPRA) revealed two bands, which exhibited virtually identical primary amino acid sequences. T cells from rPRA-immunized BALB/c mice showed a significant in vitro proliferative response to rPRA. A small but statistically significant proliferative response was also induced by rPRA in T cells from mice immunized with whole-cell coccidioidal vaccines. BALB/c mice immunized with rPRA and challenged intraperitoneally with virulent C. immitis had a greatly reduced fungal burden in their lungs and spleens compared to unvaccinated mice. The number of organisms in the lungs was reduced 500-fold, and similar reductions were observed in the spleens of immunized mice. These studies support the continued development of rPRA as a candidate vaccine for prevention of coccidioidomycosis.</description><subject>Animals</subject><subject>Antigens, Fungal - genetics</subject><subject>Antigens, Fungal - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Coccidioidomycosis - immunology</subject><subject>Coccidioidomycosis - microbiology</subject><subject>Coccidioidomycosis - prevention & control</subject><subject>Coccidiosis</subject><subject>Disease Models, Animal</subject><subject>Drug Evaluation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal and Parasitic Infections</subject><subject>Fungal Proteins</subject><subject>Fungal Vaccines - genetics</subject><subject>Fungal Vaccines - immunology</subject><subject>Gene Expression</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - immunology</subject><subject>Human protozoal diseases</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Parasitic diseases</subject><subject>Proline - immunology</subject><subject>Protozoal diseases</subject><subject>T-Lymphocytes - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFrHCEUxiW0pNu0f0Koh5LbTNXRUQ89hCVpFgK9tMcijjrZV2Z0q7ML_e_rNsvSnAqC-r7vez75IfSBkpZSpj5tbjdt37eq7QTVTScYa6nW6gKtKNGqEbXwCq0IqaIWvXyD3pbys1455-oSXepedoypFfpxd7DT3i6QIk4jXrYB73KaIIYmg9tiGxd4Cn-1dXIOPCTwoWCYZ1igYFsXPtiqxICdjR68XQKGiGdw4R16PdqphPen_Qp9v7_7tn5oHr9-2axvHxvHJV2aMTBLueqdlIKNjhPpORllGLzk2nslgvJEs3EYmOxsPSrBvA5OO0UE10N3hT4_993thzl4F-KS7WR2GWabf5tkwbxUImzNUzoYSlSnaM3fnPI5_dqHspgZigvTZGNI-2IUIR2h9fH_GWnPNVNKVKN8NrqcSslhPA9DiTkSNJWg6XujzJGgORI0R4I1ef3vX865E7Kqfzzptjg7jdlGB-VsY51mtLb5Aw0hpeM</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>KIRKLAND, T. N</creator><creator>FINLEY, F</creator><creator>ORSBORN, K. I</creator><creator>GALGIANI, J. N</creator><general>American Society for Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980801</creationdate><title>Evaluation of the proline-rich antigen of Coccidioides immitis as a vaccine candidate in mice</title><author>KIRKLAND, T. N ; FINLEY, F ; ORSBORN, K. I ; GALGIANI, J. N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-fe2a1486c7752fc407d40f7ebd749dd85e8d092fbb273ad09852d9ec9c80549b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antigens, Fungal - genetics</topic><topic>Antigens, Fungal - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Coccidioidomycosis - immunology</topic><topic>Coccidioidomycosis - microbiology</topic><topic>Coccidioidomycosis - prevention & control</topic><topic>Coccidiosis</topic><topic>Disease Models, Animal</topic><topic>Drug Evaluation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal and Parasitic Infections</topic><topic>Fungal Proteins</topic><topic>Fungal Vaccines - genetics</topic><topic>Fungal Vaccines - immunology</topic><topic>Gene Expression</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - immunology</topic><topic>Human protozoal diseases</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Parasitic diseases</topic><topic>Proline - immunology</topic><topic>Protozoal diseases</topic><topic>T-Lymphocytes - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIRKLAND, T. N</creatorcontrib><creatorcontrib>FINLEY, F</creatorcontrib><creatorcontrib>ORSBORN, K. I</creatorcontrib><creatorcontrib>GALGIANI, J. 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N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the proline-rich antigen of Coccidioides immitis as a vaccine candidate in mice</atitle><jtitle>Infection and immunity</jtitle><addtitle>Infect Immun</addtitle><date>1998-08-01</date><risdate>1998</risdate><volume>66</volume><issue>8</issue><spage>3519</spage><epage>3522</epage><pages>3519-3522</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>We have expressed the proline-rich antigen (PRA) from Coccidioides immitis in Escherichia coli and evaluated its potential as a vaccine candidate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the recombinant protein (rPRA) revealed two bands, which exhibited virtually identical primary amino acid sequences. T cells from rPRA-immunized BALB/c mice showed a significant in vitro proliferative response to rPRA. 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| ispartof | Infection and immunity, 1998-08, Vol.66 (8), p.3519-3522 |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Antigens, Fungal - genetics Antigens, Fungal - immunology Bacteriology Biological and medical sciences Cell Division Cells, Cultured Coccidioidomycosis - immunology Coccidioidomycosis - microbiology Coccidioidomycosis - prevention & control Coccidiosis Disease Models, Animal Drug Evaluation Female Fundamental and applied biological sciences. Psychology Fungal and Parasitic Infections Fungal Proteins Fungal Vaccines - genetics Fungal Vaccines - immunology Gene Expression Glycoproteins - genetics Glycoproteins - immunology Human protozoal diseases Infectious diseases Medical sciences Mice Mice, Inbred BALB C Microbiology Parasitic diseases Proline - immunology Protozoal diseases T-Lymphocytes - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - immunology |
| title | Evaluation of the proline-rich antigen of Coccidioides immitis as a vaccine candidate in mice |
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