Messenger RNAs that are not synthesized by RNA polymerase II can be 3′ end cleaved and polyadenylated
The poly(A) tail of influenza virus mRNAs is synthesized by the viral RNA polymerase by reiterative copying of a U 5–7 sequence near the 5′ end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U 6 poly(A) site with a negative‐sense eukaryotic polyadenylatio...
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| creator | Fodor, Ervin Mikulasova, Andrea Mingay, Louise J Poon, Leo L M Brownlee, George G |
| description | The poly(A) tail of influenza virus mRNAs is synthesized by the viral RNA polymerase by reiterative copying of a U
5–7
sequence near the 5′ end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U
6
poly(A) site with a negative‐sense eukaryotic polyadenylation signal. The vRNA was transcribed by the viral RNA polymerase and the transcription product was processed by the cellular 3′ end processing machinery
in vivo
. According to the current model, 3′ end processing of eukaryotic pre‐mRNAs is coupled to cellular RNA polymerase II (pol II) transcription; thus only RNAs synthesized by pol II are believed to be polyadenylated efficiently. Our results show that the cellular polyadenylation machinery is nevertheless able to recognize and process RNA transcripts that are not synthesized by pol II, indicating that synthesis by pol II is not an absolute requirement for 3′ end processing
in vivo
. |
| doi_str_mv | 10.1093/embo-reports/kvd111 |
| format | Article |
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5–7
sequence near the 5′ end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U
6
poly(A) site with a negative‐sense eukaryotic polyadenylation signal. The vRNA was transcribed by the viral RNA polymerase and the transcription product was processed by the cellular 3′ end processing machinery
in vivo
. According to the current model, 3′ end processing of eukaryotic pre‐mRNAs is coupled to cellular RNA polymerase II (pol II) transcription; thus only RNAs synthesized by pol II are believed to be polyadenylated efficiently. Our results show that the cellular polyadenylation machinery is nevertheless able to recognize and process RNA transcripts that are not synthesized by pol II, indicating that synthesis by pol II is not an absolute requirement for 3′ end processing
in vivo
.</description><identifier>ISSN: 1469-221X</identifier><identifier>EISSN: 1469-3178</identifier><identifier>DOI: 10.1093/embo-reports/kvd111</identifier><identifier>PMID: 11263496</identifier><identifier>CODEN: ERMEAX</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Base Sequence ; Cell Line ; Cloning, Molecular ; Humans ; Influenza, Human - genetics ; Molecular Sequence Data ; Mutagenesis ; Neuraminidase - genetics ; Plasmids - metabolism ; Poly A - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Polymerase II - metabolism ; RNA, Messenger - metabolism ; RNA, Viral - physiology ; RNA, Viral - ultrastructure ; Scientific Report ; Scientific Reports ; Single-Strand Specific DNA and RNA Endonucleases - metabolism ; Time Factors ; Transcription, Genetic ; Transfection</subject><ispartof>EMBO reports, 2000-12, Vol.1 (6), p.513-518</ispartof><rights>European Molecular Biology Organization 2000</rights><rights>Copyright © 2000 European Molecular Biology Organization</rights><rights>Copyright Oxford University Press(England) Dec 15, 2000</rights><rights>Copyright © 2000 European Molecular Biology Organization 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5607-7a1cec2c3f8bdf06ea3a4212a25f73b001b81e42971b7d82b013aa084e66ac373</citedby><cites>FETCH-LOGICAL-c5607-7a1cec2c3f8bdf06ea3a4212a25f73b001b81e42971b7d82b013aa084e66ac373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1083783/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1083783/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,1412,1428,27905,27906,45555,45556,46390,46814,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11263496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fodor, Ervin</creatorcontrib><creatorcontrib>Mikulasova, Andrea</creatorcontrib><creatorcontrib>Mingay, Louise J</creatorcontrib><creatorcontrib>Poon, Leo L M</creatorcontrib><creatorcontrib>Brownlee, George G</creatorcontrib><title>Messenger RNAs that are not synthesized by RNA polymerase II can be 3′ end cleaved and polyadenylated</title><title>EMBO reports</title><addtitle>EMBO Rep</addtitle><addtitle>EMBO Rep</addtitle><description>The poly(A) tail of influenza virus mRNAs is synthesized by the viral RNA polymerase by reiterative copying of a U
5–7
sequence near the 5′ end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U
6
poly(A) site with a negative‐sense eukaryotic polyadenylation signal. The vRNA was transcribed by the viral RNA polymerase and the transcription product was processed by the cellular 3′ end processing machinery
in vivo
. According to the current model, 3′ end processing of eukaryotic pre‐mRNAs is coupled to cellular RNA polymerase II (pol II) transcription; thus only RNAs synthesized by pol II are believed to be polyadenylated efficiently. Our results show that the cellular polyadenylation machinery is nevertheless able to recognize and process RNA transcripts that are not synthesized by pol II, indicating that synthesis by pol II is not an absolute requirement for 3′ end processing
in vivo
.</description><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>Humans</subject><subject>Influenza, Human - genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Neuraminidase - genetics</subject><subject>Plasmids - metabolism</subject><subject>Poly A - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Polymerase II - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Viral - physiology</subject><subject>RNA, Viral - ultrastructure</subject><subject>Scientific Report</subject><subject>Scientific Reports</subject><subject>Single-Strand Specific DNA and RNA Endonucleases - metabolism</subject><subject>Time Factors</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><issn>1469-221X</issn><issn>1469-3178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkcFu1DAQhi0EoqXwBEjI4h7qsZPYuSCVVSkrtgUVKNysSTLZTZtNtnZ223DimXgknoREiVq4IE4eyd__zz_6GXsO4hWIRB3SOm0CR5vGtf7wapcDwAO2D2GcBAq0eTjNUsK3PfbE-0shRJRo85jtAchYhUm8z5an5D3VS3L8_OzI83aFLUdHvG5a7ru6XZEvv1PO024A-KapujU59MTnc55hzVPi6tePn5zqnGcV4a6HsZ8HEnOquwpbyp-yRwVWnp5N7wH78vb48-xdsPhwMp8dLYIsioUONEJGmcxUYdK8EDGhwlCCRBkVWqVCQGqAQploSHVuZCpAIQoTUhxjprQ6YK9H3802XVOeUd06rOzGlWt0nW2wtH__1OXKLpudBWGUNqo3eDkZuOZ6S761l83W1X1mK4WJINIq6iE1QplrvHdU3C0AYYdy7FCOncqxYzm96sWf2e41Uxs9YEbgpqyo-x9Pe3z65jySw93hKPW9aqjzPva_IwWjrPQt3d5tRHdlY610ZL-enVg1u_j4fvFJ2gv1G8BBxv4</recordid><startdate>200012</startdate><enddate>200012</enddate><creator>Fodor, Ervin</creator><creator>Mikulasova, Andrea</creator><creator>Mingay, Louise J</creator><creator>Poon, Leo L M</creator><creator>Brownlee, George G</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>200012</creationdate><title>Messenger RNAs that are not synthesized by RNA polymerase II can be 3′ end cleaved and polyadenylated</title><author>Fodor, Ervin ; Mikulasova, Andrea ; Mingay, Louise J ; Poon, Leo L M ; Brownlee, George G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5607-7a1cec2c3f8bdf06ea3a4212a25f73b001b81e42971b7d82b013aa084e66ac373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>Humans</topic><topic>Influenza, Human - 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5–7
sequence near the 5′ end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U
6
poly(A) site with a negative‐sense eukaryotic polyadenylation signal. The vRNA was transcribed by the viral RNA polymerase and the transcription product was processed by the cellular 3′ end processing machinery
in vivo
. According to the current model, 3′ end processing of eukaryotic pre‐mRNAs is coupled to cellular RNA polymerase II (pol II) transcription; thus only RNAs synthesized by pol II are believed to be polyadenylated efficiently. Our results show that the cellular polyadenylation machinery is nevertheless able to recognize and process RNA transcripts that are not synthesized by pol II, indicating that synthesis by pol II is not an absolute requirement for 3′ end processing
in vivo
.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>11263496</pmid><doi>10.1093/embo-reports/kvd111</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | EMBO reports, 2000-12, Vol.1 (6), p.513-518 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; PubMed Central |
| subjects | Base Sequence Cell Line Cloning, Molecular Humans Influenza, Human - genetics Molecular Sequence Data Mutagenesis Neuraminidase - genetics Plasmids - metabolism Poly A - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA Polymerase II - metabolism RNA, Messenger - metabolism RNA, Viral - physiology RNA, Viral - ultrastructure Scientific Report Scientific Reports Single-Strand Specific DNA and RNA Endonucleases - metabolism Time Factors Transcription, Genetic Transfection |
| title | Messenger RNAs that are not synthesized by RNA polymerase II can be 3′ end cleaved and polyadenylated |
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