Phenotypic and Genetic Characterization of Clinical Isolates of CDC Coryneform Group A-3: Proposal of a New Species of Cellulomonas, Cellulomonas denverensis sp. nov
CDC coryneform group A-3 bacteria are rare human pathogens. In this study, six group A-3 isolates (two from blood, one from cerebrospinal fluid, and one each from homograft valve, lip wound, and pilonidal cyst) were compared to the type strains of phenotypically related organisms, Cellulomonas fimi,...
Gespeichert in:
| Veröffentlicht in: | Journal of Clinical Microbiology 2005-04, Vol.43 (4), p.1732-1737 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1737 |
|---|---|
| container_issue | 4 |
| container_start_page | 1732 |
| container_title | Journal of Clinical Microbiology |
| container_volume | 43 |
| creator | Brown, June M Frazier, Rodrick P Morey, Roger E Steigerwalt, Arnold G Pellegrini, Gerald J Daneshvar, Maryam I Hollis, Dannie G McNeil, Michael M |
| description | CDC coryneform group A-3 bacteria are rare human pathogens. In this study, six group A-3 isolates (two from blood, one from cerebrospinal fluid, and one each from homograft valve, lip wound, and pilonidal cyst) were compared to the type strains of phenotypically related organisms, Cellulomonas fimi, Cellulomonas hominis, Oerskovia turbata, and Sanguibacter suarezii, and characterized by phenotypic, chemotaxonomic, and genotypic studies. DNA-DNA reassociation analysis identified two genomic groups, and phylogenetic analysis of the 16S rRNA gene sequence identified the taxonomic positions of these groups to genus level. Two groups were defined, and both were more closely related to Cellulomonas species: one group of three strains, for which we propose the new species Cellulomonas denverensis sp. nov., with the type strain W6929 (ATCC BAA-788[superscript T] or DSM 15764[superscript T]), was related to C. hominis ATCC 51964[superscript T] (98.5% 16S rRNA gene sequence similarity), and the second group of three strains was related to C. hominis ATCC 51964[superscript T] (99.8 to 99.9% 16S rRNA gene sequence similarity). The definition of this new Cellulomonas species and the confirmation of three strains as C. hominis serve to further clarify the complex taxonomy of CDC coryneform group A-3 bacteria and will assist in our understanding of the epidemiology and clinical significance of these microorganisms. |
| doi_str_mv | 10.1128/JCM.43.4.1732-1737.2005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1081325</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67713623</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-2cc8227443f974e7d9d23cc6e9d68fc93afa6deeb145369dcad750f934ea3c9d3</originalsourceid><addsrcrecordid>eNqFksFu1DAQhiMEokvhFag5wIkEO3bsuAekKsBSVKBSqcTNcp3JrqvEDnZ2q-V9eE-87IqlJy5jj_3Nb4_mz7ITggtCyvrNp-ZzwWjBCiJomacgihLj6kE2I1jWOef4-8NshrGsckKoOMqexHiLMWGsqh5nR6SqCZOSzrJfl0twftqM1iDtWjQHB1PaN0sdtJkg2J96st4h36Gmt84a3aPz6Hs9Qfxz-K5BjQ8bB50PA5oHvxrRWU5P0WXwo48JT5RGX-AOXY1g7L4M-n7V-8E7HV_fy1ALbg0BXLQRxbFAzq-fZo863Ud4tl-Ps-sP7781H_OLr_Pz5uwiN1XJp7w0pi5LwRjtpGAgWtmW1BgOsuV1ZyTVneYtwA1hFeWyNboVFe4kZaCpkS09zt7udMfVzQCtATcF3asx2EGHjfLaqvs3zi7Vwq8VwTWhZZUEXu0Fgv-xgjipwUaTutMO_CoqLgShvKT_BYlgklKOEyh2oAk-xgDd398QrLZeUMkLilHF1NYL2yDU1gup8vm_zRzq9sNPwMs9oGMaaxe0MzYeOM5rgWueuBc7bmkXyzsbQOk4qFszHJ5NzMmO6bRXehGSzvVViQlNHpS8xjX9DXpG1RQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17493360</pqid></control><display><type>article</type><title>Phenotypic and Genetic Characterization of Clinical Isolates of CDC Coryneform Group A-3: Proposal of a New Species of Cellulomonas, Cellulomonas denverensis sp. nov</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Brown, June M ; Frazier, Rodrick P ; Morey, Roger E ; Steigerwalt, Arnold G ; Pellegrini, Gerald J ; Daneshvar, Maryam I ; Hollis, Dannie G ; McNeil, Michael M</creator><creatorcontrib>Brown, June M ; Frazier, Rodrick P ; Morey, Roger E ; Steigerwalt, Arnold G ; Pellegrini, Gerald J ; Daneshvar, Maryam I ; Hollis, Dannie G ; McNeil, Michael M</creatorcontrib><description>CDC coryneform group A-3 bacteria are rare human pathogens. In this study, six group A-3 isolates (two from blood, one from cerebrospinal fluid, and one each from homograft valve, lip wound, and pilonidal cyst) were compared to the type strains of phenotypically related organisms, Cellulomonas fimi, Cellulomonas hominis, Oerskovia turbata, and Sanguibacter suarezii, and characterized by phenotypic, chemotaxonomic, and genotypic studies. DNA-DNA reassociation analysis identified two genomic groups, and phylogenetic analysis of the 16S rRNA gene sequence identified the taxonomic positions of these groups to genus level. Two groups were defined, and both were more closely related to Cellulomonas species: one group of three strains, for which we propose the new species Cellulomonas denverensis sp. nov., with the type strain W6929 (ATCC BAA-788[superscript T] or DSM 15764[superscript T]), was related to C. hominis ATCC 51964[superscript T] (98.5% 16S rRNA gene sequence similarity), and the second group of three strains was related to C. hominis ATCC 51964[superscript T] (99.8 to 99.9% 16S rRNA gene sequence similarity). The definition of this new Cellulomonas species and the confirmation of three strains as C. hominis serve to further clarify the complex taxonomy of CDC coryneform group A-3 bacteria and will assist in our understanding of the epidemiology and clinical significance of these microorganisms.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.43.4.1732-1737.2005</identifier><identifier>PMID: 15814993</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Actinomycetales Infections - microbiology ; Bacterial Typing Techniques ; Bacteriology ; Biological and medical sciences ; Cellulomonas - chemistry ; Cellulomonas - classification ; Cellulomonas - genetics ; Cellulomonas - isolation & purification ; Cellulomonas denverensis ; Cellulomonas fimi ; Cellulomonas hominis ; DNA, Ribosomal - analysis ; Fatty Acids - analysis ; Fundamental and applied biological sciences. Psychology ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Molecular Sequence Data ; Oerskovia turbata ; Phenotype ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sanguibacter suarezii ; Sequence Analysis, DNA</subject><ispartof>Journal of Clinical Microbiology, 2005-04, Vol.43 (4), p.1732-1737</ispartof><rights>2005 INIST-CNRS</rights><rights>2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-2cc8227443f974e7d9d23cc6e9d68fc93afa6deeb145369dcad750f934ea3c9d3</citedby><cites>FETCH-LOGICAL-c526t-2cc8227443f974e7d9d23cc6e9d68fc93afa6deeb145369dcad750f934ea3c9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081325/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081325/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16687086$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15814993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, June M</creatorcontrib><creatorcontrib>Frazier, Rodrick P</creatorcontrib><creatorcontrib>Morey, Roger E</creatorcontrib><creatorcontrib>Steigerwalt, Arnold G</creatorcontrib><creatorcontrib>Pellegrini, Gerald J</creatorcontrib><creatorcontrib>Daneshvar, Maryam I</creatorcontrib><creatorcontrib>Hollis, Dannie G</creatorcontrib><creatorcontrib>McNeil, Michael M</creatorcontrib><title>Phenotypic and Genetic Characterization of Clinical Isolates of CDC Coryneform Group A-3: Proposal of a New Species of Cellulomonas, Cellulomonas denverensis sp. nov</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>CDC coryneform group A-3 bacteria are rare human pathogens. In this study, six group A-3 isolates (two from blood, one from cerebrospinal fluid, and one each from homograft valve, lip wound, and pilonidal cyst) were compared to the type strains of phenotypically related organisms, Cellulomonas fimi, Cellulomonas hominis, Oerskovia turbata, and Sanguibacter suarezii, and characterized by phenotypic, chemotaxonomic, and genotypic studies. DNA-DNA reassociation analysis identified two genomic groups, and phylogenetic analysis of the 16S rRNA gene sequence identified the taxonomic positions of these groups to genus level. Two groups were defined, and both were more closely related to Cellulomonas species: one group of three strains, for which we propose the new species Cellulomonas denverensis sp. nov., with the type strain W6929 (ATCC BAA-788[superscript T] or DSM 15764[superscript T]), was related to C. hominis ATCC 51964[superscript T] (98.5% 16S rRNA gene sequence similarity), and the second group of three strains was related to C. hominis ATCC 51964[superscript T] (99.8 to 99.9% 16S rRNA gene sequence similarity). The definition of this new Cellulomonas species and the confirmation of three strains as C. hominis serve to further clarify the complex taxonomy of CDC coryneform group A-3 bacteria and will assist in our understanding of the epidemiology and clinical significance of these microorganisms.</description><subject>Actinomycetales Infections - microbiology</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cellulomonas - chemistry</subject><subject>Cellulomonas - classification</subject><subject>Cellulomonas - genetics</subject><subject>Cellulomonas - isolation & purification</subject><subject>Cellulomonas denverensis</subject><subject>Cellulomonas fimi</subject><subject>Cellulomonas hominis</subject><subject>DNA, Ribosomal - analysis</subject><subject>Fatty Acids - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Oerskovia turbata</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sanguibacter suarezii</subject><subject>Sequence Analysis, DNA</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksFu1DAQhiMEokvhFag5wIkEO3bsuAekKsBSVKBSqcTNcp3JrqvEDnZ2q-V9eE-87IqlJy5jj_3Nb4_mz7ITggtCyvrNp-ZzwWjBCiJomacgihLj6kE2I1jWOef4-8NshrGsckKoOMqexHiLMWGsqh5nR6SqCZOSzrJfl0twftqM1iDtWjQHB1PaN0sdtJkg2J96st4h36Gmt84a3aPz6Hs9Qfxz-K5BjQ8bB50PA5oHvxrRWU5P0WXwo48JT5RGX-AOXY1g7L4M-n7V-8E7HV_fy1ALbg0BXLQRxbFAzq-fZo863Ud4tl-Ps-sP7781H_OLr_Pz5uwiN1XJp7w0pi5LwRjtpGAgWtmW1BgOsuV1ZyTVneYtwA1hFeWyNboVFe4kZaCpkS09zt7udMfVzQCtATcF3asx2EGHjfLaqvs3zi7Vwq8VwTWhZZUEXu0Fgv-xgjipwUaTutMO_CoqLgShvKT_BYlgklKOEyh2oAk-xgDd398QrLZeUMkLilHF1NYL2yDU1gup8vm_zRzq9sNPwMs9oGMaaxe0MzYeOM5rgWueuBc7bmkXyzsbQOk4qFszHJ5NzMmO6bRXehGSzvVViQlNHpS8xjX9DXpG1RQ</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Brown, June M</creator><creator>Frazier, Rodrick P</creator><creator>Morey, Roger E</creator><creator>Steigerwalt, Arnold G</creator><creator>Pellegrini, Gerald J</creator><creator>Daneshvar, Maryam I</creator><creator>Hollis, Dannie G</creator><creator>McNeil, Michael M</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050401</creationdate><title>Phenotypic and Genetic Characterization of Clinical Isolates of CDC Coryneform Group A-3: Proposal of a New Species of Cellulomonas, Cellulomonas denverensis sp. nov</title><author>Brown, June M ; Frazier, Rodrick P ; Morey, Roger E ; Steigerwalt, Arnold G ; Pellegrini, Gerald J ; Daneshvar, Maryam I ; Hollis, Dannie G ; McNeil, Michael M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-2cc8227443f974e7d9d23cc6e9d68fc93afa6deeb145369dcad750f934ea3c9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Actinomycetales Infections - microbiology</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cellulomonas - chemistry</topic><topic>Cellulomonas - classification</topic><topic>Cellulomonas - genetics</topic><topic>Cellulomonas - isolation & purification</topic><topic>Cellulomonas denverensis</topic><topic>Cellulomonas fimi</topic><topic>Cellulomonas hominis</topic><topic>DNA, Ribosomal - analysis</topic><topic>Fatty Acids - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Oerskovia turbata</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sanguibacter suarezii</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, June M</creatorcontrib><creatorcontrib>Frazier, Rodrick P</creatorcontrib><creatorcontrib>Morey, Roger E</creatorcontrib><creatorcontrib>Steigerwalt, Arnold G</creatorcontrib><creatorcontrib>Pellegrini, Gerald J</creatorcontrib><creatorcontrib>Daneshvar, Maryam I</creatorcontrib><creatorcontrib>Hollis, Dannie G</creatorcontrib><creatorcontrib>McNeil, Michael M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, June M</au><au>Frazier, Rodrick P</au><au>Morey, Roger E</au><au>Steigerwalt, Arnold G</au><au>Pellegrini, Gerald J</au><au>Daneshvar, Maryam I</au><au>Hollis, Dannie G</au><au>McNeil, Michael M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and Genetic Characterization of Clinical Isolates of CDC Coryneform Group A-3: Proposal of a New Species of Cellulomonas, Cellulomonas denverensis sp. nov</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>43</volume><issue>4</issue><spage>1732</spage><epage>1737</epage><pages>1732-1737</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>CDC coryneform group A-3 bacteria are rare human pathogens. In this study, six group A-3 isolates (two from blood, one from cerebrospinal fluid, and one each from homograft valve, lip wound, and pilonidal cyst) were compared to the type strains of phenotypically related organisms, Cellulomonas fimi, Cellulomonas hominis, Oerskovia turbata, and Sanguibacter suarezii, and characterized by phenotypic, chemotaxonomic, and genotypic studies. DNA-DNA reassociation analysis identified two genomic groups, and phylogenetic analysis of the 16S rRNA gene sequence identified the taxonomic positions of these groups to genus level. Two groups were defined, and both were more closely related to Cellulomonas species: one group of three strains, for which we propose the new species Cellulomonas denverensis sp. nov., with the type strain W6929 (ATCC BAA-788[superscript T] or DSM 15764[superscript T]), was related to C. hominis ATCC 51964[superscript T] (98.5% 16S rRNA gene sequence similarity), and the second group of three strains was related to C. hominis ATCC 51964[superscript T] (99.8 to 99.9% 16S rRNA gene sequence similarity). The definition of this new Cellulomonas species and the confirmation of three strains as C. hominis serve to further clarify the complex taxonomy of CDC coryneform group A-3 bacteria and will assist in our understanding of the epidemiology and clinical significance of these microorganisms.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>15814993</pmid><doi>10.1128/JCM.43.4.1732-1737.2005</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0095-1137 |
| ispartof | Journal of Clinical Microbiology, 2005-04, Vol.43 (4), p.1732-1737 |
| issn | 0095-1137 1098-660X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1081325 |
| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Actinomycetales Infections - microbiology Bacterial Typing Techniques Bacteriology Biological and medical sciences Cellulomonas - chemistry Cellulomonas - classification Cellulomonas - genetics Cellulomonas - isolation & purification Cellulomonas denverensis Cellulomonas fimi Cellulomonas hominis DNA, Ribosomal - analysis Fatty Acids - analysis Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Microbiology Molecular Sequence Data Oerskovia turbata Phenotype Phylogeny RNA, Ribosomal, 16S - genetics Sanguibacter suarezii Sequence Analysis, DNA |
| title | Phenotypic and Genetic Characterization of Clinical Isolates of CDC Coryneform Group A-3: Proposal of a New Species of Cellulomonas, Cellulomonas denverensis sp. nov |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T02%3A39%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phenotypic%20and%20Genetic%20Characterization%20of%20Clinical%20Isolates%20of%20CDC%20Coryneform%20Group%20A-3:%20Proposal%20of%20a%20New%20Species%20of%20Cellulomonas,%20Cellulomonas%20denverensis%20sp.%20nov&rft.jtitle=Journal%20of%20Clinical%20Microbiology&rft.au=Brown,%20June%20M&rft.date=2005-04-01&rft.volume=43&rft.issue=4&rft.spage=1732&rft.epage=1737&rft.pages=1732-1737&rft.issn=0095-1137&rft.eissn=1098-660X&rft.coden=JCMIDW&rft_id=info:doi/10.1128/JCM.43.4.1732-1737.2005&rft_dat=%3Cproquest_pubme%3E67713623%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17493360&rft_id=info:pmid/15814993&rfr_iscdi=true |