Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats
Objectives Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics. Reference data have only been established for feline peripheral blood. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs)...
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| Veröffentlicht in: | Journal of feline medicine and surgery 2022-02, Vol.24 (2), p.77-90 |
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| creator | Rütgen, Barbara C Baszler, Elisabeth Weingand, Nicole Wolfesberger, Birgitt Baumgartner, Daniel Hammer, Sabine E Groiss, Sandra Fuchs-Baumgartinger, Andrea Saalmüller, Armin Schwendenwein, Ilse |
| description | Objectives
Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics. Reference data have only been established for feline peripheral blood. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs) are lacking. The aim of this prospective study was to establish reference data for lymphocyte subpopulations in normal and mildly reactive feline peripheral LNs using a standardised multicolour panel of antibodies.
Methods
Macroscopically inconspicuous mandibular and/or popliteal LNs from 31 adult cats, which were euthanased for reasons other than haematological diseases, were excised and processed within 5 h after death. Multicolour flow cytometry using eight different feline-specific, anti-canine and human cross-reactive monoclonal antibodies used in current diagnostic marker panels was performed after cytological exclusion of pathological states and complemented by lymphocyte clonality testing, histopathology and immunohistochemistry (IHC) to ensure the absence of lymphoid disease.
Results
Of 31 cats, the immunophenotyping data of 24 individuals could be included as histopathology and clonality testing excluded a pathological condition. Lymphocyte populations showed the following positive antibody reactions: CD18+ 86.3% ± 13.86%, CD3+ 54.81% ± 11.10%, CD5+ 57.39% ± 12.66%, CD21+ 40.42% ± 12.40%, CD79alphacy+ (CD79αcy) 30.41% ± 13.49% and CD14+ 0.75% ± 1.35%. There were 30.88% ± 13.48% CD4+ and 12.91% ± 6.68% CD8+ cells. Cytology revealed a mixed population of mostly lymphoid cells in all samples. The absence of a monoclonal/oligoclonal neoplastic population was confirmed by lymphocyte clonality testing. Histopathology and IHC showed a normal or mildly reactive pattern in all cases.
Conclusions and relevance
This study establishes FCM immunophenotyping data of lymphocyte populations of normal and mildly reactive feline peripheral LNs. For the first time, anti-CD5, CD4, CD8 and CD21 reference data in normal and mildly reactive feline peripheral LNs are presented. CD18, CD3, CD14 and CD79αcy have been used to establish reference data for the first time in any feline material. |
| doi_str_mv | 10.1177/1098612X211005310 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_AFRWT</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10812179</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_1098612X211005310</sage_id><sourcerecordid>2519311980</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-7b76d2ef9c940a20eb4c5e9baa423b7ada57898f58d182c2f822455a5b0a5c6d3</originalsourceid><addsrcrecordid>eNp9kU9r3DAQxUVJadK0H6CXoGMuTmek1Vo6hbCkfyDQSwu9CVkeZxVsy5HswH77arNpSCj0NIL3e28GPcY-IVwg1vVnBKPXKH4LRAAlEd6wE1RSVKJWcFTeRa_2wDF7n_MdABhpxDt2LKUBrRFOGG3iMMUc5hBHHjve74ZpG_1uJp6XZorT0ru9lnkY-RjT4HruxpYPoW_7HU_k_BweiE-UwrSlVOTHiMK29Gjybs4f2NvO9Zk-Ps1T9uvL9c_Nt-rmx9fvm6ubykuznqu6qdetoM54swIngJqVV2Qa51ZCNrVrnaq10Z3SLWrhRaeFWCnlVANO-XUrT9nlIXdamoFaT-NcLrJTCoNLOxtdsK-VMWztbXywCBoF1qYknD8lpHi_UJ7tELKnvncjxSVbodBIRKOhoHhAfYo5J-qe9yDYfT_2n36K5-zlgc-Ov4UU4OIAZHdL9i4uaSwf9p_EPwIzm5s</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2519311980</pqid></control><display><type>article</type><title>Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats</title><source>Sage Journals GOLD Open Access 2024</source><creator>Rütgen, Barbara C ; Baszler, Elisabeth ; Weingand, Nicole ; Wolfesberger, Birgitt ; Baumgartner, Daniel ; Hammer, Sabine E ; Groiss, Sandra ; Fuchs-Baumgartinger, Andrea ; Saalmüller, Armin ; Schwendenwein, Ilse</creator><creatorcontrib>Rütgen, Barbara C ; Baszler, Elisabeth ; Weingand, Nicole ; Wolfesberger, Birgitt ; Baumgartner, Daniel ; Hammer, Sabine E ; Groiss, Sandra ; Fuchs-Baumgartinger, Andrea ; Saalmüller, Armin ; Schwendenwein, Ilse</creatorcontrib><description>Objectives
Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics. Reference data have only been established for feline peripheral blood. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs) are lacking. The aim of this prospective study was to establish reference data for lymphocyte subpopulations in normal and mildly reactive feline peripheral LNs using a standardised multicolour panel of antibodies.
Methods
Macroscopically inconspicuous mandibular and/or popliteal LNs from 31 adult cats, which were euthanased for reasons other than haematological diseases, were excised and processed within 5 h after death. Multicolour flow cytometry using eight different feline-specific, anti-canine and human cross-reactive monoclonal antibodies used in current diagnostic marker panels was performed after cytological exclusion of pathological states and complemented by lymphocyte clonality testing, histopathology and immunohistochemistry (IHC) to ensure the absence of lymphoid disease.
Results
Of 31 cats, the immunophenotyping data of 24 individuals could be included as histopathology and clonality testing excluded a pathological condition. Lymphocyte populations showed the following positive antibody reactions: CD18+ 86.3% ± 13.86%, CD3+ 54.81% ± 11.10%, CD5+ 57.39% ± 12.66%, CD21+ 40.42% ± 12.40%, CD79alphacy+ (CD79αcy) 30.41% ± 13.49% and CD14+ 0.75% ± 1.35%. There were 30.88% ± 13.48% CD4+ and 12.91% ± 6.68% CD8+ cells. Cytology revealed a mixed population of mostly lymphoid cells in all samples. The absence of a monoclonal/oligoclonal neoplastic population was confirmed by lymphocyte clonality testing. Histopathology and IHC showed a normal or mildly reactive pattern in all cases.
Conclusions and relevance
This study establishes FCM immunophenotyping data of lymphocyte populations of normal and mildly reactive feline peripheral LNs. For the first time, anti-CD5, CD4, CD8 and CD21 reference data in normal and mildly reactive feline peripheral LNs are presented. CD18, CD3, CD14 and CD79αcy have been used to establish reference data for the first time in any feline material.</description><identifier>ISSN: 1098-612X</identifier><identifier>ISSN: 1532-2750</identifier><identifier>EISSN: 1532-2750</identifier><identifier>DOI: 10.1177/1098612X211005310</identifier><identifier>PMID: 33908810</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Antibodies, Monoclonal ; Cats ; Flow Cytometry - veterinary ; Immunophenotyping - veterinary ; Lymph Nodes - cytology ; Lymphocyte Subsets ; Original ; Prospective Studies</subject><ispartof>Journal of feline medicine and surgery, 2022-02, Vol.24 (2), p.77-90</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021 2021 International Society of Feline Medicine and American Association of Feline Practitioners</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-7b76d2ef9c940a20eb4c5e9baa423b7ada57898f58d182c2f822455a5b0a5c6d3</citedby><cites>FETCH-LOGICAL-c396t-7b76d2ef9c940a20eb4c5e9baa423b7ada57898f58d182c2f822455a5b0a5c6d3</cites><orcidid>0000-0001-9080-0164</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812179/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812179/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,21945,27830,27901,27902,44921,45309,53766,53768</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/1098612X211005310?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33908810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rütgen, Barbara C</creatorcontrib><creatorcontrib>Baszler, Elisabeth</creatorcontrib><creatorcontrib>Weingand, Nicole</creatorcontrib><creatorcontrib>Wolfesberger, Birgitt</creatorcontrib><creatorcontrib>Baumgartner, Daniel</creatorcontrib><creatorcontrib>Hammer, Sabine E</creatorcontrib><creatorcontrib>Groiss, Sandra</creatorcontrib><creatorcontrib>Fuchs-Baumgartinger, Andrea</creatorcontrib><creatorcontrib>Saalmüller, Armin</creatorcontrib><creatorcontrib>Schwendenwein, Ilse</creatorcontrib><title>Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats</title><title>Journal of feline medicine and surgery</title><addtitle>J Feline Med Surg</addtitle><description>Objectives
Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics. Reference data have only been established for feline peripheral blood. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs) are lacking. The aim of this prospective study was to establish reference data for lymphocyte subpopulations in normal and mildly reactive feline peripheral LNs using a standardised multicolour panel of antibodies.
Methods
Macroscopically inconspicuous mandibular and/or popliteal LNs from 31 adult cats, which were euthanased for reasons other than haematological diseases, were excised and processed within 5 h after death. Multicolour flow cytometry using eight different feline-specific, anti-canine and human cross-reactive monoclonal antibodies used in current diagnostic marker panels was performed after cytological exclusion of pathological states and complemented by lymphocyte clonality testing, histopathology and immunohistochemistry (IHC) to ensure the absence of lymphoid disease.
Results
Of 31 cats, the immunophenotyping data of 24 individuals could be included as histopathology and clonality testing excluded a pathological condition. Lymphocyte populations showed the following positive antibody reactions: CD18+ 86.3% ± 13.86%, CD3+ 54.81% ± 11.10%, CD5+ 57.39% ± 12.66%, CD21+ 40.42% ± 12.40%, CD79alphacy+ (CD79αcy) 30.41% ± 13.49% and CD14+ 0.75% ± 1.35%. There were 30.88% ± 13.48% CD4+ and 12.91% ± 6.68% CD8+ cells. Cytology revealed a mixed population of mostly lymphoid cells in all samples. The absence of a monoclonal/oligoclonal neoplastic population was confirmed by lymphocyte clonality testing. Histopathology and IHC showed a normal or mildly reactive pattern in all cases.
Conclusions and relevance
This study establishes FCM immunophenotyping data of lymphocyte populations of normal and mildly reactive feline peripheral LNs. For the first time, anti-CD5, CD4, CD8 and CD21 reference data in normal and mildly reactive feline peripheral LNs are presented. CD18, CD3, CD14 and CD79αcy have been used to establish reference data for the first time in any feline material.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Cats</subject><subject>Flow Cytometry - veterinary</subject><subject>Immunophenotyping - veterinary</subject><subject>Lymph Nodes - cytology</subject><subject>Lymphocyte Subsets</subject><subject>Original</subject><subject>Prospective Studies</subject><issn>1098-612X</issn><issn>1532-2750</issn><issn>1532-2750</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9r3DAQxUVJadK0H6CXoGMuTmek1Vo6hbCkfyDQSwu9CVkeZxVsy5HswH77arNpSCj0NIL3e28GPcY-IVwg1vVnBKPXKH4LRAAlEd6wE1RSVKJWcFTeRa_2wDF7n_MdABhpxDt2LKUBrRFOGG3iMMUc5hBHHjve74ZpG_1uJp6XZorT0ru9lnkY-RjT4HruxpYPoW_7HU_k_BweiE-UwrSlVOTHiMK29Gjybs4f2NvO9Zk-Ps1T9uvL9c_Nt-rmx9fvm6ubykuznqu6qdetoM54swIngJqVV2Qa51ZCNrVrnaq10Z3SLWrhRaeFWCnlVANO-XUrT9nlIXdamoFaT-NcLrJTCoNLOxtdsK-VMWztbXywCBoF1qYknD8lpHi_UJ7tELKnvncjxSVbodBIRKOhoHhAfYo5J-qe9yDYfT_2n36K5-zlgc-Ov4UU4OIAZHdL9i4uaSwf9p_EPwIzm5s</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Rütgen, Barbara C</creator><creator>Baszler, Elisabeth</creator><creator>Weingand, Nicole</creator><creator>Wolfesberger, Birgitt</creator><creator>Baumgartner, Daniel</creator><creator>Hammer, Sabine E</creator><creator>Groiss, Sandra</creator><creator>Fuchs-Baumgartinger, Andrea</creator><creator>Saalmüller, Armin</creator><creator>Schwendenwein, Ilse</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9080-0164</orcidid></search><sort><creationdate>20220201</creationdate><title>Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats</title><author>Rütgen, Barbara C ; Baszler, Elisabeth ; Weingand, Nicole ; Wolfesberger, Birgitt ; Baumgartner, Daniel ; Hammer, Sabine E ; Groiss, Sandra ; Fuchs-Baumgartinger, Andrea ; Saalmüller, Armin ; Schwendenwein, Ilse</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-7b76d2ef9c940a20eb4c5e9baa423b7ada57898f58d182c2f822455a5b0a5c6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Cats</topic><topic>Flow Cytometry - veterinary</topic><topic>Immunophenotyping - veterinary</topic><topic>Lymph Nodes - cytology</topic><topic>Lymphocyte Subsets</topic><topic>Original</topic><topic>Prospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rütgen, Barbara C</creatorcontrib><creatorcontrib>Baszler, Elisabeth</creatorcontrib><creatorcontrib>Weingand, Nicole</creatorcontrib><creatorcontrib>Wolfesberger, Birgitt</creatorcontrib><creatorcontrib>Baumgartner, Daniel</creatorcontrib><creatorcontrib>Hammer, Sabine E</creatorcontrib><creatorcontrib>Groiss, Sandra</creatorcontrib><creatorcontrib>Fuchs-Baumgartinger, Andrea</creatorcontrib><creatorcontrib>Saalmüller, Armin</creatorcontrib><creatorcontrib>Schwendenwein, Ilse</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of feline medicine and surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Rütgen, Barbara C</au><au>Baszler, Elisabeth</au><au>Weingand, Nicole</au><au>Wolfesberger, Birgitt</au><au>Baumgartner, Daniel</au><au>Hammer, Sabine E</au><au>Groiss, Sandra</au><au>Fuchs-Baumgartinger, Andrea</au><au>Saalmüller, Armin</au><au>Schwendenwein, Ilse</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats</atitle><jtitle>Journal of feline medicine and surgery</jtitle><addtitle>J Feline Med Surg</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>24</volume><issue>2</issue><spage>77</spage><epage>90</epage><pages>77-90</pages><issn>1098-612X</issn><issn>1532-2750</issn><eissn>1532-2750</eissn><abstract>Objectives
Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics. Reference data have only been established for feline peripheral blood. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs) are lacking. The aim of this prospective study was to establish reference data for lymphocyte subpopulations in normal and mildly reactive feline peripheral LNs using a standardised multicolour panel of antibodies.
Methods
Macroscopically inconspicuous mandibular and/or popliteal LNs from 31 adult cats, which were euthanased for reasons other than haematological diseases, were excised and processed within 5 h after death. Multicolour flow cytometry using eight different feline-specific, anti-canine and human cross-reactive monoclonal antibodies used in current diagnostic marker panels was performed after cytological exclusion of pathological states and complemented by lymphocyte clonality testing, histopathology and immunohistochemistry (IHC) to ensure the absence of lymphoid disease.
Results
Of 31 cats, the immunophenotyping data of 24 individuals could be included as histopathology and clonality testing excluded a pathological condition. Lymphocyte populations showed the following positive antibody reactions: CD18+ 86.3% ± 13.86%, CD3+ 54.81% ± 11.10%, CD5+ 57.39% ± 12.66%, CD21+ 40.42% ± 12.40%, CD79alphacy+ (CD79αcy) 30.41% ± 13.49% and CD14+ 0.75% ± 1.35%. There were 30.88% ± 13.48% CD4+ and 12.91% ± 6.68% CD8+ cells. Cytology revealed a mixed population of mostly lymphoid cells in all samples. The absence of a monoclonal/oligoclonal neoplastic population was confirmed by lymphocyte clonality testing. Histopathology and IHC showed a normal or mildly reactive pattern in all cases.
Conclusions and relevance
This study establishes FCM immunophenotyping data of lymphocyte populations of normal and mildly reactive feline peripheral LNs. For the first time, anti-CD5, CD4, CD8 and CD21 reference data in normal and mildly reactive feline peripheral LNs are presented. CD18, CD3, CD14 and CD79αcy have been used to establish reference data for the first time in any feline material.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>33908810</pmid><doi>10.1177/1098612X211005310</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-9080-0164</orcidid></addata></record> |
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| ispartof | Journal of feline medicine and surgery, 2022-02, Vol.24 (2), p.77-90 |
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| source | Sage Journals GOLD Open Access 2024 |
| subjects | Animals Antibodies, Monoclonal Cats Flow Cytometry - veterinary Immunophenotyping - veterinary Lymph Nodes - cytology Lymphocyte Subsets Original Prospective Studies |
| title | Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats |
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