Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells
To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adher...
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| Veröffentlicht in: | Infection and immunity 1998-04, Vol.66 (4), p.1783-1786 |
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| container_issue | 4 |
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| container_title | Infection and immunity |
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| creator | YUE FU RIEG, G FONZI, W. A BELANGER, P. H EDWARDS, J. E FILLER, S. G |
| description | To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1. These results indicate that ALS1 may encode a candidal adhesin. |
| doi_str_mv | 10.1128/iai.66.4.1783-1786.1998 |
| format | Article |
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These results indicate that ALS1 may encode a candidal adhesin.</description><subject>Adhesiveness</subject><subject>Biological and medical sciences</subject><subject>Candida albicans - genetics</subject><subject>Endothelium, Vascular - microbiology</subject><subject>Epithelial Cells - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal and Parasitic Infections</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Genes, Fungal</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - physiology</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUuP0zAQthBoKQs_AeED4pbgcZyHDxyqaoFKlTgsnK2JM9kapXax0xV74bfjaEsFJy4zmvkemtHH2BsQJYDs3jt0ZdOUqoS2q4pcmhK07p6wFQjdFXUt5VO2EgJ0oeumfc5epPQ9j0qp7opd6VpqALViv25-HiOl5ILnYeTznvgG_eAG5Dj1zqJP_I488fXuFrjz_Bat3WMMhwdLiVuKdO-SQ8rYcFpWOOzz0lvic-Dkh5A9J4cTz7acju7PaGma0kv2bMQp0atzv2bfPt583Xwudl8-bTfrXWFVC3MB0iLKbhyGVjZKVrbrLAjsbStIiqpXYIfeZkY99n01jLKVetSiIiWznqi6Zh8efY-n_kCDJT9HnMwxugPGBxPQmX8R7_bmLtwbEB1IyPp3Z30MP06UZnNwafkAPYVTMq1ua91J-V8iNDUIUS_E9pFoY0gp0ng5BoRZIjbb9dY0jVFmiXgpjVkizsrXf_9y0Z0zzfjbM47J4jRG9NalCy0fCZlZ_QZwYrK7</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>YUE FU</creator><creator>RIEG, G</creator><creator>FONZI, W. 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| ispartof | Infection and immunity, 1998-04, Vol.66 (4), p.1783-1786 |
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| language | eng |
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| source | Open Access: PubMed Central; MEDLINE; American Society for Microbiology Journals; Free E-Journal (出版社公開部分のみ） |
| subjects | Adhesiveness Biological and medical sciences Candida albicans - genetics Endothelium, Vascular - microbiology Epithelial Cells - microbiology Fundamental and applied biological sciences. Psychology Fungal and Parasitic Infections Fungal Proteins - chemistry Fungal Proteins - genetics Genes, Fungal Growth, nutrition, metabolism, transports, enzymes. Molecular biology Humans Microbiology Mycology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - physiology |
| title | Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells |
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