Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var Columbia
Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, isoleucine, and valine, is inhibited by imidazolinone herbicides. To understand the molecular basis of imidazolinone resistance, we isolated the ALS gene from an imazapyr-resistant mutant GH90 of Arabidopsis thalia...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1991-11, Vol.97 (3), p.1044-1050 |
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| creator | Sathasivan, K. (The University of Texas at Austin, Austin, TX) Haughn, G.W Murai, N |
| description | Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, isoleucine, and valine, is inhibited by imidazolinone herbicides. To understand the molecular basis of imidazolinone resistance, we isolated the ALS gene from an imazapyr-resistant mutant GH90 of Arabidopsis thaliana. DNA sequence analysis of the mutant ALS gene demonstrated a single-point mutation from G to A at nucleotide 1958 of the ALS-coding sequence. This would result in Ser to Asn substitution at residue 653 near the carboxyl terminal of the matured ALS. The mutant ALS gene was introduced into tobacco using Agrobacterium-mediated transformation. Imidazolinone-resistant growth of transformed calli and leaves of transgenic plants was 100-fold greater than that of nontransformed control plants. The relative levels of imidazolinone-resistant ALS activity correlated with the amount of herbicide-resistant growth in the leaves of transgenic plants. Southern hybridization analysis confirmed the existence of transferred ALS gene in the transformant showing high imazapyr resistance. The results demonstrate that the mutant ALS gene confers resistance to imidazolinone herbicides. This is the first report, to our knowledge, of the molecular basis of imidazolinone resistance in plants |
| doi_str_mv | 10.1104/pp.97.3.1044 |
| format | Article |
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(The University of Texas at Austin, Austin, TX) ; Haughn, G.W ; Murai, N</creator><creatorcontrib>Sathasivan, K. (The University of Texas at Austin, Austin, TX) ; Haughn, G.W ; Murai, N</creatorcontrib><description>Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, isoleucine, and valine, is inhibited by imidazolinone herbicides. To understand the molecular basis of imidazolinone resistance, we isolated the ALS gene from an imazapyr-resistant mutant GH90 of Arabidopsis thaliana. DNA sequence analysis of the mutant ALS gene demonstrated a single-point mutation from G to A at nucleotide 1958 of the ALS-coding sequence. This would result in Ser to Asn substitution at residue 653 near the carboxyl terminal of the matured ALS. The mutant ALS gene was introduced into tobacco using Agrobacterium-mediated transformation. Imidazolinone-resistant growth of transformed calli and leaves of transgenic plants was 100-fold greater than that of nontransformed control plants. The relative levels of imidazolinone-resistant ALS activity correlated with the amount of herbicide-resistant growth in the leaves of transgenic plants. Southern hybridization analysis confirmed the existence of transferred ALS gene in the transformant showing high imazapyr resistance. The results demonstrate that the mutant ALS gene confers resistance to imidazolinone herbicides. This is the first report, to our knowledge, of the molecular basis of imidazolinone resistance in plants</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.97.3.1044</identifier><identifier>PMID: 16668488</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Agronomy. Soil science and plant productions ; AMELIORATION DES PLANTES ; Biological and medical sciences ; Callus ; Cross resistance ; CRUCIFERAE ; DNA ; FITOMEJORAMIENTO ; Fundamental and applied biological sciences. Psychology ; Genetic mutation ; Genetics and breeding of economic plants ; HERBICIDAS ; HERBICIDE ; Herbicide resistance ; Herbicides ; Leaves ; Molecular Biology and Gene Regulation ; Mutagenesis ; MUTANT ; MUTANTES ; Plant breeding: fundamental aspects and methodology ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants ; Plasmids ; RESISTANCE AUX PRODUITS CHIMIQUES ; RESISTENCIA QUIMICA ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Transgenic plants ; VARIACION GENETICA ; VARIATION GENETIQUE</subject><ispartof>Plant physiology (Bethesda), 1991-11, Vol.97 (3), p.1044-1050</ispartof><rights>Copyright 1991 American Society of Plant Physiologists</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-e792eca6cbafd8accafb8d6756df3588083b4d706522083359ad0ff9e41e68aa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4273948$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4273948$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5555727$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16668488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sathasivan, K. (The University of Texas at Austin, Austin, TX)</creatorcontrib><creatorcontrib>Haughn, G.W</creatorcontrib><creatorcontrib>Murai, N</creatorcontrib><title>Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var Columbia</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, isoleucine, and valine, is inhibited by imidazolinone herbicides. To understand the molecular basis of imidazolinone resistance, we isolated the ALS gene from an imazapyr-resistant mutant GH90 of Arabidopsis thaliana. DNA sequence analysis of the mutant ALS gene demonstrated a single-point mutation from G to A at nucleotide 1958 of the ALS-coding sequence. This would result in Ser to Asn substitution at residue 653 near the carboxyl terminal of the matured ALS. The mutant ALS gene was introduced into tobacco using Agrobacterium-mediated transformation. Imidazolinone-resistant growth of transformed calli and leaves of transgenic plants was 100-fold greater than that of nontransformed control plants. The relative levels of imidazolinone-resistant ALS activity correlated with the amount of herbicide-resistant growth in the leaves of transgenic plants. Southern hybridization analysis confirmed the existence of transferred ALS gene in the transformant showing high imazapyr resistance. The results demonstrate that the mutant ALS gene confers resistance to imidazolinone herbicides. This is the first report, to our knowledge, of the molecular basis of imidazolinone resistance in plants</description><subject>Agronomy. Soil science and plant productions</subject><subject>AMELIORATION DES PLANTES</subject><subject>Biological and medical sciences</subject><subject>Callus</subject><subject>Cross resistance</subject><subject>CRUCIFERAE</subject><subject>DNA</subject><subject>FITOMEJORAMIENTO</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic mutation</subject><subject>Genetics and breeding of economic plants</subject><subject>HERBICIDAS</subject><subject>HERBICIDE</subject><subject>Herbicide resistance</subject><subject>Herbicides</subject><subject>Leaves</subject><subject>Molecular Biology and Gene Regulation</subject><subject>Mutagenesis</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants</subject><subject>Plasmids</subject><subject>RESISTANCE AUX PRODUITS CHIMIQUES</subject><subject>RESISTENCIA QUIMICA</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>Transgenic plants</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNpVkc9rFDEcxYNY7Fq9eRKROQhe3DWZJDPJRShLq4WKB-3FS_hOfnRTMpNpMlPQv74ZdlltLnnwPnnf8H0IvSF4Qwhmn8dxI9sN3RTNnqEV4bRe15yJ52iFcdFYCHmKXuZ8hzEmlLAX6JQ0TSOYECv0-3sMVs8BUtVB9rmKrvK9N_A3Bj_EwVY7mzqvvbFVsgWYYNC28kN1nqDzJo7Lo2kHwcMA1UPJ2cYw952HV-jEQcj29eE-QzeXF7-239bXP75ebc-v15rzelrbVtZWQ6M7cEaA1uA6YZqWN8ZRLgQWtGOmxQ2v66Ipl2Cwc9IyYhsBQM_Ql33uOHe9NdoOU4KgxuR7SH9UBK-eOoPfqdv4oAgWhNSkBHw8BKR4P9s8qd5nbUOAwcY5q5ZSJhlhC_lpT-oUc07WHacQrJY21Dgq2SqqljYK_v7_n_2DD-svwIcDAFlDcKks1-cjx8tp67Zg7_bYXZ5iOtqsWJItKW_3toOo4DaVhJufkjSkNE8fAbwEpp8</recordid><startdate>19911101</startdate><enddate>19911101</enddate><creator>Sathasivan, K. (The University of Texas at Austin, Austin, TX)</creator><creator>Haughn, G.W</creator><creator>Murai, N</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19911101</creationdate><title>Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var Columbia</title><author>Sathasivan, K. (The University of Texas at Austin, Austin, TX) ; Haughn, G.W ; Murai, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c552t-e792eca6cbafd8accafb8d6756df3588083b4d706522083359ad0ff9e41e68aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>AMELIORATION DES PLANTES</topic><topic>Biological and medical sciences</topic><topic>Callus</topic><topic>Cross resistance</topic><topic>CRUCIFERAE</topic><topic>DNA</topic><topic>FITOMEJORAMIENTO</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic mutation</topic><topic>Genetics and breeding of economic plants</topic><topic>HERBICIDAS</topic><topic>HERBICIDE</topic><topic>Herbicide resistance</topic><topic>Herbicides</topic><topic>Leaves</topic><topic>Molecular Biology and Gene Regulation</topic><topic>Mutagenesis</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants</topic><topic>Plasmids</topic><topic>RESISTANCE AUX PRODUITS CHIMIQUES</topic><topic>RESISTENCIA QUIMICA</topic><topic>TRANSFERENCIA DE GENES</topic><topic>TRANSFERT DE GENE</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>Transgenic plants</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sathasivan, K. (The University of Texas at Austin, Austin, TX)</creatorcontrib><creatorcontrib>Haughn, G.W</creatorcontrib><creatorcontrib>Murai, N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sathasivan, K. (The University of Texas at Austin, Austin, TX)</au><au>Haughn, G.W</au><au>Murai, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var Columbia</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1991-11-01</date><risdate>1991</risdate><volume>97</volume><issue>3</issue><spage>1044</spage><epage>1050</epage><pages>1044-1050</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, isoleucine, and valine, is inhibited by imidazolinone herbicides. To understand the molecular basis of imidazolinone resistance, we isolated the ALS gene from an imazapyr-resistant mutant GH90 of Arabidopsis thaliana. DNA sequence analysis of the mutant ALS gene demonstrated a single-point mutation from G to A at nucleotide 1958 of the ALS-coding sequence. This would result in Ser to Asn substitution at residue 653 near the carboxyl terminal of the matured ALS. The mutant ALS gene was introduced into tobacco using Agrobacterium-mediated transformation. Imidazolinone-resistant growth of transformed calli and leaves of transgenic plants was 100-fold greater than that of nontransformed control plants. The relative levels of imidazolinone-resistant ALS activity correlated with the amount of herbicide-resistant growth in the leaves of transgenic plants. Southern hybridization analysis confirmed the existence of transferred ALS gene in the transformant showing high imazapyr resistance. The results demonstrate that the mutant ALS gene confers resistance to imidazolinone herbicides. This is the first report, to our knowledge, of the molecular basis of imidazolinone resistance in plants</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16668488</pmid><doi>10.1104/pp.97.3.1044</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1991-11, Vol.97 (3), p.1044-1050 |
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| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Agronomy. Soil science and plant productions AMELIORATION DES PLANTES Biological and medical sciences Callus Cross resistance CRUCIFERAE DNA FITOMEJORAMIENTO Fundamental and applied biological sciences. Psychology Genetic mutation Genetics and breeding of economic plants HERBICIDAS HERBICIDE Herbicide resistance Herbicides Leaves Molecular Biology and Gene Regulation Mutagenesis MUTANT MUTANTES Plant breeding: fundamental aspects and methodology PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants Plasmids RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA QUIMICA TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transgenic plants VARIACION GENETICA VARIATION GENETIQUE |
| title | Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var Columbia |
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