A recombinant live attenuated strain of Vibrio cholerae induces immunity against tetanus toxin and Bordetella pertussis tracheal colonization factor
An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor from Bordetella pertussis (Tcf). In vitro, high levels of protein were obtained when the Escherichia coli nirB promo...
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| Veröffentlicht in: | Infection and immunity 1998-04, Vol.66 (4), p.1648-1653 |
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| creator | CHEN, I FINN, T. M LIU YANQING QI GUOMING RAPPUOLI, R PIZZA, M |
| description | An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor from Bordetella pertussis (Tcf). In vitro, high levels of protein were obtained when the Escherichia coli nirB promoter was used and the bacteria were grown with low aeration. Intranasal immunization of mice with IEM101 expressing TetC elicited serum vibriocidal activity and induced antibodies against tetanus toxin which were protective against lethal challenge with 10 times the 50% lethal dose of tetanus toxin. Bacterial viability was essential for the induction of anti-TetC antibodies. Intranasal administration of IEM101 expressing Tcf induced a significant reduction in bacterial colonization of the tracheas of mice challenged with wild-type B. pertussis. These data are in agreement with the putative role of Tcf in Bordetella tracheal colonization. In conclusion, we have demonstrated that V. cholerae may be used as a live vector to deliver heterologous antigens in vivo and that protection to both systemic and local challenge may be achieved. |
| doi_str_mv | 10.1128/iai.66.4.1648-1653.1998 |
| format | Article |
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M ; LIU YANQING ; QI GUOMING ; RAPPUOLI, R ; PIZZA, M</creator><creatorcontrib>CHEN, I ; FINN, T. M ; LIU YANQING ; QI GUOMING ; RAPPUOLI, R ; PIZZA, M</creatorcontrib><description>An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor from Bordetella pertussis (Tcf). In vitro, high levels of protein were obtained when the Escherichia coli nirB promoter was used and the bacteria were grown with low aeration. Intranasal immunization of mice with IEM101 expressing TetC elicited serum vibriocidal activity and induced antibodies against tetanus toxin which were protective against lethal challenge with 10 times the 50% lethal dose of tetanus toxin. Bacterial viability was essential for the induction of anti-TetC antibodies. Intranasal administration of IEM101 expressing Tcf induced a significant reduction in bacterial colonization of the tracheas of mice challenged with wild-type B. pertussis. These data are in agreement with the putative role of Tcf in Bordetella tracheal colonization. In conclusion, we have demonstrated that V. cholerae may be used as a live vector to deliver heterologous antigens in vivo and that protection to both systemic and local challenge may be achieved.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/iai.66.4.1648-1653.1998</identifier><identifier>PMID: 9529093</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Administration, Intranasal ; Animals ; Antibodies, Bacterial - biosynthesis ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Bacterial Proteins - physiology ; Bacteriology ; Biological and medical sciences ; Female ; Fundamental and applied biological sciences. Psychology ; Host Response and Inflammation ; Immunization ; Mice ; Mice, Inbred BALB C ; Microbiology ; Peptide Fragments - genetics ; Peptide Fragments - immunology ; Recombinant Proteins - immunology ; Tetanus Toxin - genetics ; Tetanus Toxin - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vibrio cholerae - genetics ; Virulence Factors, Bordetella</subject><ispartof>Infection and immunity, 1998-04, Vol.66 (4), p.1648-1653</ispartof><rights>1998 INIST-CNRS</rights><rights>Copyright © 1998, American Society for Microbiology 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-a18a7ff0452c2916e4e91efb7c07d6c568a918a6e24c7b0b6006f50841692b543</citedby><cites>FETCH-LOGICAL-c471t-a18a7ff0452c2916e4e91efb7c07d6c568a918a6e24c7b0b6006f50841692b543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC108100/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC108100/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2228953$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9529093$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHEN, I</creatorcontrib><creatorcontrib>FINN, T. M</creatorcontrib><creatorcontrib>LIU YANQING</creatorcontrib><creatorcontrib>QI GUOMING</creatorcontrib><creatorcontrib>RAPPUOLI, R</creatorcontrib><creatorcontrib>PIZZA, M</creatorcontrib><title>A recombinant live attenuated strain of Vibrio cholerae induces immunity against tetanus toxin and Bordetella pertussis tracheal colonization factor</title><title>Infection and immunity</title><addtitle>Infect Immun</addtitle><description>An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor from Bordetella pertussis (Tcf). In vitro, high levels of protein were obtained when the Escherichia coli nirB promoter was used and the bacteria were grown with low aeration. Intranasal immunization of mice with IEM101 expressing TetC elicited serum vibriocidal activity and induced antibodies against tetanus toxin which were protective against lethal challenge with 10 times the 50% lethal dose of tetanus toxin. Bacterial viability was essential for the induction of anti-TetC antibodies. Intranasal administration of IEM101 expressing Tcf induced a significant reduction in bacterial colonization of the tracheas of mice challenged with wild-type B. pertussis. These data are in agreement with the putative role of Tcf in Bordetella tracheal colonization. In conclusion, we have demonstrated that V. cholerae may be used as a live vector to deliver heterologous antigens in vivo and that protection to both systemic and local challenge may be achieved.</description><subject>Administration, Intranasal</subject><subject>Animals</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Host Response and Inflammation</subject><subject>Immunization</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Peptide Fragments - genetics</subject><subject>Peptide Fragments - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Tetanus Toxin - genetics</subject><subject>Tetanus Toxin - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vibrio cholerae - genetics</subject><subject>Virulence Factors, Bordetella</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcFu1DAURSMEKkPhExBeIHYZbMd27AWLoaIwUiU2wNZ6cV46Rok92E5F-Q4-uIk6GsHKsu659ns6VfWG0S1jXL_34LdKbcWWKaFrpmSzZcboJ9WGUaNrKTl_Wm0oZaY2UrXPqxc5_1yuQgh9UV0YyQ01zab6uyMJXZw6HyAUMvo7JFAKhhkK9iSXBD6QOJAfvks-EneIIyZA4kM_O8zET9McfLkncLuQuZCCBcKcSYm_lyaEnnyMqceC4wjkiKnMOfslTuAOCCNxcYzB_4HiYyADuBLTy-rZAGPGV6fzsvp-_enb1Zf65uvn_dXupnaiZaUGpqEdBiokd9wwhQINw6FrHW175aTSYBZEIReu7WinKFWDpFowZXgnRXNZfXh89zh3E_YOwzLVaI_JT5DubQRv_0-CP9jbeGcZ1YzSpf_u1E_x14y52Mlnty4aMM7ZMsVN03C2gO0j6FLMOeFw_oNRu_q0-93eKmWFXX3a1addfS7N1_-OeO6dBC7521MO2cE4JAjO5zPGOddGNs0DEgCukg</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>CHEN, I</creator><creator>FINN, T. 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M ; LIU YANQING ; QI GUOMING ; RAPPUOLI, R ; PIZZA, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-a18a7ff0452c2916e4e91efb7c07d6c568a918a6e24c7b0b6006f50841692b543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Administration, Intranasal</topic><topic>Animals</topic><topic>Antibodies, Bacterial - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Proteins - physiology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Host Response and Inflammation</topic><topic>Immunization</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Peptide Fragments - genetics</topic><topic>Peptide Fragments - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Tetanus Toxin - genetics</topic><topic>Tetanus Toxin - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vibrio cholerae - genetics</topic><topic>Virulence Factors, Bordetella</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHEN, I</creatorcontrib><creatorcontrib>FINN, T. 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M</au><au>LIU YANQING</au><au>QI GUOMING</au><au>RAPPUOLI, R</au><au>PIZZA, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A recombinant live attenuated strain of Vibrio cholerae induces immunity against tetanus toxin and Bordetella pertussis tracheal colonization factor</atitle><jtitle>Infection and immunity</jtitle><addtitle>Infect Immun</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>66</volume><issue>4</issue><spage>1648</spage><epage>1653</epage><pages>1648-1653</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor from Bordetella pertussis (Tcf). In vitro, high levels of protein were obtained when the Escherichia coli nirB promoter was used and the bacteria were grown with low aeration. Intranasal immunization of mice with IEM101 expressing TetC elicited serum vibriocidal activity and induced antibodies against tetanus toxin which were protective against lethal challenge with 10 times the 50% lethal dose of tetanus toxin. Bacterial viability was essential for the induction of anti-TetC antibodies. Intranasal administration of IEM101 expressing Tcf induced a significant reduction in bacterial colonization of the tracheas of mice challenged with wild-type B. pertussis. These data are in agreement with the putative role of Tcf in Bordetella tracheal colonization. In conclusion, we have demonstrated that V. cholerae may be used as a live vector to deliver heterologous antigens in vivo and that protection to both systemic and local challenge may be achieved.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9529093</pmid><doi>10.1128/iai.66.4.1648-1653.1998</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Infection and immunity, 1998-04, Vol.66 (4), p.1648-1653 |
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| language | eng |
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| source | MEDLINE; American Society for Microbiology Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Administration, Intranasal Animals Antibodies, Bacterial - biosynthesis Bacterial Proteins - genetics Bacterial Proteins - immunology Bacterial Proteins - physiology Bacteriology Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Host Response and Inflammation Immunization Mice Mice, Inbred BALB C Microbiology Peptide Fragments - genetics Peptide Fragments - immunology Recombinant Proteins - immunology Tetanus Toxin - genetics Tetanus Toxin - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vibrio cholerae - genetics Virulence Factors, Bordetella |
| title | A recombinant live attenuated strain of Vibrio cholerae induces immunity against tetanus toxin and Bordetella pertussis tracheal colonization factor |
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