Symport of proton and sucrose in plasma membrane vesicles isolated from spinach leaves
The mechanism of sucrose transport was investigated in plasma membrane (PM) vesicles isolated from spinach (Spinacia oleracea L.) leaves. PM vesicles were isolated by aqueous two-phase partitioning and were equilibrated in pH 7.8 buffer containing K+. The vesicles rapidly accumulated sucrose in the...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1991-06, Vol.96 (2), p.615-618 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Slone, J.H. (Climate Stress Laboratory, Beltsville Agricultural Research Center, USDA, ARS, Beltsville, MD) Buckout, T.J VanDerWoude, W.J |
| description | The mechanism of sucrose transport was investigated in plasma membrane (PM) vesicles isolated from spinach (Spinacia oleracea L.) leaves. PM vesicles were isolated by aqueous two-phase partitioning and were equilibrated in pH 7.8 buffer containing K+. The vesicles rapidly accumulated sucrose in the presence of a transmembrane pH gradient (deltapH) with external pH set at 5.8. The uptake rate was slow at pH 7.8. The K+-selective ionophore, valinomycin, stimulated uptake in the presence of a deltapH, and the protonophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP), greatly inhibited deltapH-dependent sucrose uptake. Addition of sucrose to the vesicles resulted in immediate alkalization of the medium. Alkalization was stimulated by valinomycin, was abolished by CCCP, and was sucrose-specific. These results demonstrate the presence of a tightly coupled H+/sucrose symporter in PM vesicles isolated from spinach leaves |
| doi_str_mv | 10.1104/pp.96.2.615 |
| format | Article |
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Addition of sucrose to the vesicles resulted in immediate alkalization of the medium. Alkalization was stimulated by valinomycin, was abolished by CCCP, and was sucrose-specific. These results demonstrate the presence of a tightly coupled H+/sucrose symporter in PM vesicles isolated from spinach leaves</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.96.2.615</identifier><identifier>PMID: 16668230</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Biological and medical sciences ; CARBOHIDRATOS ; Cell membranes ; Cell physiology ; ESTRUCTURA CELULAR ; Ethanol ; FEUILLE ; FISIOLOGIA VEGETAL ; FOTOSINTESIS ; Fundamental and applied biological sciences. Psychology ; GLUCIDE ; HOJAS ; Leaves ; membrane vesicles ; Membranes and Bioenergetics ; Phloem loading ; PHOTOSYNTHESE ; PHYSIOLOGIE VEGETALE ; Plant physiology and development ; Plant roots ; Plants ; Plasma membrane and permeation ; plasma membranes ; Protons ; SACCHAROSE ; Spinach ; SPINACIA OLERACEA ; STRUCTURE CELLULAIRE ; SUCROSA ; sucrose ; Sugar beets ; Sugars ; TRANSPORT DES SUBSTANCES NUTRITIVES ; TRANSPORTE DE NUTRIENTES</subject><ispartof>Plant physiology (Bethesda), 1991-06, Vol.96 (2), p.615-618</ispartof><rights>Copyright 1991 American Society of Plant Physiologists</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-f849b89ca9b0b3affb9a2a620e2e134c67379a4bb6df7748ac6485f21f780aa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4273647$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4273647$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5026269$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16668230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Slone, J.H. (Climate Stress Laboratory, Beltsville Agricultural Research Center, USDA, ARS, Beltsville, MD)</creatorcontrib><creatorcontrib>Buckout, T.J</creatorcontrib><creatorcontrib>VanDerWoude, W.J</creatorcontrib><title>Symport of proton and sucrose in plasma membrane vesicles isolated from spinach leaves</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The mechanism of sucrose transport was investigated in plasma membrane (PM) vesicles isolated from spinach (Spinacia oleracea L.) leaves. PM vesicles were isolated by aqueous two-phase partitioning and were equilibrated in pH 7.8 buffer containing K+. The vesicles rapidly accumulated sucrose in the presence of a transmembrane pH gradient (deltapH) with external pH set at 5.8. The uptake rate was slow at pH 7.8. The K+-selective ionophore, valinomycin, stimulated uptake in the presence of a deltapH, and the protonophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP), greatly inhibited deltapH-dependent sucrose uptake. Addition of sucrose to the vesicles resulted in immediate alkalization of the medium. Alkalization was stimulated by valinomycin, was abolished by CCCP, and was sucrose-specific. These results demonstrate the presence of a tightly coupled H+/sucrose symporter in PM vesicles isolated from spinach leaves</description><subject>Biological and medical sciences</subject><subject>CARBOHIDRATOS</subject><subject>Cell membranes</subject><subject>Cell physiology</subject><subject>ESTRUCTURA CELULAR</subject><subject>Ethanol</subject><subject>FEUILLE</subject><subject>FISIOLOGIA VEGETAL</subject><subject>FOTOSINTESIS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLUCIDE</subject><subject>HOJAS</subject><subject>Leaves</subject><subject>membrane vesicles</subject><subject>Membranes and Bioenergetics</subject><subject>Phloem loading</subject><subject>PHOTOSYNTHESE</subject><subject>PHYSIOLOGIE VEGETALE</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>Plants</subject><subject>Plasma membrane and permeation</subject><subject>plasma membranes</subject><subject>Protons</subject><subject>SACCHAROSE</subject><subject>Spinach</subject><subject>SPINACIA OLERACEA</subject><subject>STRUCTURE CELLULAIRE</subject><subject>SUCROSA</subject><subject>sucrose</subject><subject>Sugar beets</subject><subject>Sugars</subject><subject>TRANSPORT DES SUBSTANCES NUTRITIVES</subject><subject>TRANSPORTE DE NUTRIENTES</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9kc9rFDEcxYNY7Lp68iYiOYgeZNf8mkxyEaSoLRQ8tHoN38kmbUpmkiazhf73Td1l1YunBN6Hx_u-h9ArStaUEvEp57WWa7aWtHuCFrTjbMU6oZ6iBSHtT5TSx-h5rTeEEMqpeIaOqZRSMU4W6NfF_ZhTmXHyOJc0pwnDtMF1a0uqDocJ5wh1BDy6cSgwOXznarDRVRxqijC7DfYljbjmMIG9xtFBI16gIw-xupf7d4kuv329PDldnf_4fnby5XxlO8rnlVdCD0pb0AMZOHg_aGAgGXHMUS6s7HmvQQyD3Pi-FwqsFKrzjPpeEQC-RJ93tnk7jG5j3TQXiCaXMEK5NwmC-VeZwrW5SneGEkVUq2qJPuwNSrrdujqbMVTrYmyXpm01PedCC01FI9__l6Sd1lpR1cCPO_CxwVqcP8ShxDwOZnI2Whpm5O8Ab_--4A-7X6gB7_YAVAvRtw1sqAeuI0wyqRv2Zofd1DmVgyxYz6Xom_x6J3tIBq5Kc_h5oVvFvCV-AJhNsWU</recordid><startdate>19910601</startdate><enddate>19910601</enddate><creator>Slone, J.H. (Climate Stress Laboratory, Beltsville Agricultural Research Center, USDA, ARS, Beltsville, MD)</creator><creator>Buckout, T.J</creator><creator>VanDerWoude, W.J</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910601</creationdate><title>Symport of proton and sucrose in plasma membrane vesicles isolated from spinach leaves</title><author>Slone, J.H. (Climate Stress Laboratory, Beltsville Agricultural Research Center, USDA, ARS, Beltsville, MD) ; Buckout, T.J ; VanDerWoude, W.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-f849b89ca9b0b3affb9a2a620e2e134c67379a4bb6df7748ac6485f21f780aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Biological and medical sciences</topic><topic>CARBOHIDRATOS</topic><topic>Cell membranes</topic><topic>Cell physiology</topic><topic>ESTRUCTURA CELULAR</topic><topic>Ethanol</topic><topic>FEUILLE</topic><topic>FISIOLOGIA VEGETAL</topic><topic>FOTOSINTESIS</topic><topic>Fundamental and applied biological sciences. 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(Climate Stress Laboratory, Beltsville Agricultural Research Center, USDA, ARS, Beltsville, MD)</creatorcontrib><creatorcontrib>Buckout, T.J</creatorcontrib><creatorcontrib>VanDerWoude, W.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slone, J.H. (Climate Stress Laboratory, Beltsville Agricultural Research Center, USDA, ARS, Beltsville, MD)</au><au>Buckout, T.J</au><au>VanDerWoude, W.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Symport of proton and sucrose in plasma membrane vesicles isolated from spinach leaves</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>96</volume><issue>2</issue><spage>615</spage><epage>618</epage><pages>615-618</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The mechanism of sucrose transport was investigated in plasma membrane (PM) vesicles isolated from spinach (Spinacia oleracea L.) leaves. PM vesicles were isolated by aqueous two-phase partitioning and were equilibrated in pH 7.8 buffer containing K+. The vesicles rapidly accumulated sucrose in the presence of a transmembrane pH gradient (deltapH) with external pH set at 5.8. The uptake rate was slow at pH 7.8. The K+-selective ionophore, valinomycin, stimulated uptake in the presence of a deltapH, and the protonophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP), greatly inhibited deltapH-dependent sucrose uptake. Addition of sucrose to the vesicles resulted in immediate alkalization of the medium. Alkalization was stimulated by valinomycin, was abolished by CCCP, and was sucrose-specific. These results demonstrate the presence of a tightly coupled H+/sucrose symporter in PM vesicles isolated from spinach leaves</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16668230</pmid><doi>10.1104/pp.96.2.615</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1991-06, Vol.96 (2), p.615-618 |
| issn | 0032-0889 1532-2548 |
| language | eng |
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| source | Jstor Complete Legacy; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Biological and medical sciences CARBOHIDRATOS Cell membranes Cell physiology ESTRUCTURA CELULAR Ethanol FEUILLE FISIOLOGIA VEGETAL FOTOSINTESIS Fundamental and applied biological sciences. Psychology GLUCIDE HOJAS Leaves membrane vesicles Membranes and Bioenergetics Phloem loading PHOTOSYNTHESE PHYSIOLOGIE VEGETALE Plant physiology and development Plant roots Plants Plasma membrane and permeation plasma membranes Protons SACCHAROSE Spinach SPINACIA OLERACEA STRUCTURE CELLULAIRE SUCROSA sucrose Sugar beets Sugars TRANSPORT DES SUBSTANCES NUTRITIVES TRANSPORTE DE NUTRIENTES |
| title | Symport of proton and sucrose in plasma membrane vesicles isolated from spinach leaves |
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