MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment
Reconstruction of irregular oral-maxillofacial bone defects with an inflammatory microenvironment remains a challenge, as chronic local inflammation can largely impair bone healing. Here, we used magnesium silicate nanospheres (MSNs) to load microRNA-146a-5p (miR-146a) to fabricate a nanobiomaterial...
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| Veröffentlicht in: | Bone Research 2024-01, Vol.12 (1), p.2-2, Article 2 |
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| creator | Yang, Jiakang Shuai, Jing Siow, Lixuen Lu, Jingyi Sun, Miao An, Wenyue Yu, Mengfei Wang, Baixiang Chen, Qianming |
| description | Reconstruction of irregular oral-maxillofacial bone defects with an inflammatory microenvironment remains a challenge, as chronic local inflammation can largely impair bone healing. Here, we used magnesium silicate nanospheres (MSNs) to load microRNA-146a-5p (miR-146a) to fabricate a nanobiomaterial, MSN+miR-146a, which showed synergistic promoting effects on the osteogenic differentiation of human dental pulp stem cells (hDPSCs). In addition, miR-146a exhibited an anti-inflammatory effect on mouse bone marrow-derived macrophages (BMMs) under lipopolysaccharide (LPS) stimulation by inhibiting the NF-κB pathway via targeting tumor necrosis factor receptor-associated factor 6 (TRAF6), and MSNs could simultaneously promote M2 polarization of BMMs. MiR-146a was also found to inhibit osteoclast formation. Finally, the dual osteogenic-promoting and immunoregulatory effects of MSN+miR-146a were further validated in a stimulated infected mouse mandibular bone defect model via delivery by a photocuring hydrogel. Collectively, the MSN+miR-146a complex revealed good potential in treating inflammatory irregular oral-maxillofacial bone defects. |
| doi_str_mv | 10.1038/s41413-023-00299-0 |
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Here, we used magnesium silicate nanospheres (MSNs) to load microRNA-146a-5p (miR-146a) to fabricate a nanobiomaterial, MSN+miR-146a, which showed synergistic promoting effects on the osteogenic differentiation of human dental pulp stem cells (hDPSCs). In addition, miR-146a exhibited an anti-inflammatory effect on mouse bone marrow-derived macrophages (BMMs) under lipopolysaccharide (LPS) stimulation by inhibiting the NF-κB pathway via targeting tumor necrosis factor receptor-associated factor 6 (TRAF6), and MSNs could simultaneously promote M2 polarization of BMMs. MiR-146a was also found to inhibit osteoclast formation. Finally, the dual osteogenic-promoting and immunoregulatory effects of MSN+miR-146a were further validated in a stimulated infected mouse mandibular bone defect model via delivery by a photocuring hydrogel. Collectively, the MSN+miR-146a complex revealed good potential in treating inflammatory irregular oral-maxillofacial bone defects.</description><identifier>ISSN: 2095-4700</identifier><identifier>ISSN: 2095-6231</identifier><identifier>EISSN: 2095-6231</identifier><identifier>DOI: 10.1038/s41413-023-00299-0</identifier><identifier>PMID: 38221522</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/443/63 ; 631/443/811 ; Arthritis ; Bone marrow ; Bone surgery ; Defects ; Gum disease ; Inflammation ; Internal Medicine ; Kinases ; Medicine ; Medicine & Public Health ; MicroRNAs ; Microscopy ; Morphology ; Nanomaterials ; Orthopedics ; Silica ; Stem cells</subject><ispartof>Bone Research, 2024-01, Vol.12 (1), p.2-2, Article 2</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-aeff8331a76b853b5a6ee7a003386783ac609d86561452bda80c2e795c1aa2dd3</citedby><cites>FETCH-LOGICAL-c541t-aeff8331a76b853b5a6ee7a003386783ac609d86561452bda80c2e795c1aa2dd3</cites><orcidid>0000-0003-2922-7615 ; 0000-0002-7034-4189</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10788347/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10788347/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38221522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Jiakang</creatorcontrib><creatorcontrib>Shuai, Jing</creatorcontrib><creatorcontrib>Siow, Lixuen</creatorcontrib><creatorcontrib>Lu, Jingyi</creatorcontrib><creatorcontrib>Sun, Miao</creatorcontrib><creatorcontrib>An, Wenyue</creatorcontrib><creatorcontrib>Yu, Mengfei</creatorcontrib><creatorcontrib>Wang, Baixiang</creatorcontrib><creatorcontrib>Chen, Qianming</creatorcontrib><title>MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment</title><title>Bone Research</title><addtitle>Bone Res</addtitle><addtitle>Bone Res</addtitle><description>Reconstruction of irregular oral-maxillofacial bone defects with an inflammatory microenvironment remains a challenge, as chronic local inflammation can largely impair bone healing. Here, we used magnesium silicate nanospheres (MSNs) to load microRNA-146a-5p (miR-146a) to fabricate a nanobiomaterial, MSN+miR-146a, which showed synergistic promoting effects on the osteogenic differentiation of human dental pulp stem cells (hDPSCs). In addition, miR-146a exhibited an anti-inflammatory effect on mouse bone marrow-derived macrophages (BMMs) under lipopolysaccharide (LPS) stimulation by inhibiting the NF-κB pathway via targeting tumor necrosis factor receptor-associated factor 6 (TRAF6), and MSNs could simultaneously promote M2 polarization of BMMs. MiR-146a was also found to inhibit osteoclast formation. Finally, the dual osteogenic-promoting and immunoregulatory effects of MSN+miR-146a were further validated in a stimulated infected mouse mandibular bone defect model via delivery by a photocuring hydrogel. Collectively, the MSN+miR-146a complex revealed good potential in treating inflammatory irregular oral-maxillofacial bone defects.</description><subject>631/443/63</subject><subject>631/443/811</subject><subject>Arthritis</subject><subject>Bone marrow</subject><subject>Bone surgery</subject><subject>Defects</subject><subject>Gum disease</subject><subject>Inflammation</subject><subject>Internal Medicine</subject><subject>Kinases</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MicroRNAs</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Nanomaterials</subject><subject>Orthopedics</subject><subject>Silica</subject><subject>Stem cells</subject><issn>2095-4700</issn><issn>2095-6231</issn><issn>2095-6231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNp9kk1v1DAQhiMEolXpH-CAInHhEhh_Jc4JVRUflQpICM7WxJmkXiX21s5W6r_Hu1kK5cDBHxo_845n9BbFSwZvGQj9LkkmmaiA5wW8bSt4UpxyaFVVc8GeHu-yATgpzlPaAADjWrZaPC9OhOacKc5Pi9svzsbw_etFxWSN1RSwp76ccfSU3G4uk5ucxYVKjz6k7Q1FSuU2hjnkWBc8lZFG8hRxccGXzpe434cJ5xmXEO_LeV-A_J2Lwc_klxfFswGnROfH86z4-fHDj8vP1fW3T1eXF9eVVZItFdIwaCEYNnWnlegU1kQNAgih60YLtDW0va5VzaTiXY8aLKemVZYh8r4XZ8XVqtsH3JhtdDPGexPQmUMgxNFgXJydyFio-4E6zQlaCb3KBUFZ0QyEjVWCstb7VWu762bqbW4j4vRI9PGLdzdmDHeGQaO1kE1WeHNUiOF2R2kxs0uWpgk9hV0yvGWSq1rxNqOv_0E3YRd9ntWByv03WmaKr1SebkqRhoffMDB7h5jVISY7xBwcYiAnvfq7j4eU337IgFiBlJ_8SPFP7f_I_gKIsshF</recordid><startdate>20240115</startdate><enddate>20240115</enddate><creator>Yang, Jiakang</creator><creator>Shuai, Jing</creator><creator>Siow, Lixuen</creator><creator>Lu, Jingyi</creator><creator>Sun, Miao</creator><creator>An, Wenyue</creator><creator>Yu, Mengfei</creator><creator>Wang, Baixiang</creator><creator>Chen, Qianming</creator><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2922-7615</orcidid><orcidid>https://orcid.org/0000-0002-7034-4189</orcidid></search><sort><creationdate>20240115</creationdate><title>MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment</title><author>Yang, Jiakang ; 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Collectively, the MSN+miR-146a complex revealed good potential in treating inflammatory irregular oral-maxillofacial bone defects.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>38221522</pmid><doi>10.1038/s41413-023-00299-0</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2922-7615</orcidid><orcidid>https://orcid.org/0000-0002-7034-4189</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 631/443/63 631/443/811 Arthritis Bone marrow Bone surgery Defects Gum disease Inflammation Internal Medicine Kinases Medicine Medicine & Public Health MicroRNAs Microscopy Morphology Nanomaterials Orthopedics Silica Stem cells |
| title | MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment |
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