Stimulation of Pro-inflammatory Cytokines in Mixed Cultures of Peripheral Blood Mononuclear Cells and Anaerobic Bacteria
In the last couple of decades, much progress has been made in studying bacteria living in humans. However, there is much more to learn about bacteria immune cell interactions. Here, we show that anaerobic bacteria do not grow when cultured overnight with human cells under atmospheric air. Air contai...
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| Veröffentlicht in: | Curēus (Palo Alto, CA) CA), 2023-12, Vol.15 (12), p.e50586-e50586 |
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| creator | Torres, Anthony R Morris, Shayne Benson, Michael Wilkinson, Craig Lyon, Rachael |
| description | In the last couple of decades, much progress has been made in studying bacteria living in humans. However, there is much more to learn about bacteria immune cell interactions. Here, we show that anaerobic bacteria do not grow when cultured overnight with human cells under atmospheric air. Air contains about 18% oxygen, which inhibits the growth of these bacteria while supporting the cultivation of human cells. The bacteria cultured with human peripheral blood mononuclear cells (PBMCs) inflamed with phytohemagglutinin (PHA) greatly increased the production of proinflammatory cytokines like tumor necrosis factor-alpha (TNFα) while inhibiting the production of monocyte chemoattractant protein-1 (MCP-1), an important chemokine. |
| doi_str_mv | 10.7759/cureus.50586 |
| format | Article |
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| subjects | Allergy/Immunology Bacteria Cell culture Chemokines Cytokines DNA fingerprints Females Genetics Inflammation Metabolites Microbiota Microorganisms Probiotics Sheep Variance analysis |
| title | Stimulation of Pro-inflammatory Cytokines in Mixed Cultures of Peripheral Blood Mononuclear Cells and Anaerobic Bacteria |
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