Lactate dehydrogenase in Oryza sativa L. seedlings and roots. Identification and partial characterization
A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots. Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots. In 30 day old roots, the activity is increased two to three times by an anoxic or...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1991-03, Vol.95 (3), p.682-686 |
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| creator | Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI) Ricard, B Pradet, A |
| description | A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots. Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots. In 30 day old roots, the activity is increased two to three times by an anoxic or hypoxic treatment and can be detected on immunoblots by an antiserum raised against barley lactate dehydrogenase. The activity present in aerobic seedlings was partially purified. The native enzyme has a molecular mass of 160 kilodaltons, and is a tetramer of 2 subunit (38 and 39 kilodaltons) randomly associated. Studies of substrate specificity, native gel electrophoresis, and immunoblot analysis indicate that the partially purified enzyme is a typical lactate dehydrogenase. However, no increase of lactate dehydrogenase activity or protein was observed in seedlings transferred to anoxia |
| doi_str_mv | 10.1104/pp.95.3.682 |
| format | Article |
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Identification and partial characterization</title><source>Jstor Complete Legacy</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI) ; Ricard, B ; Pradet, A</creator><creatorcontrib>Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI) ; Ricard, B ; Pradet, A</creatorcontrib><description>A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots. Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots. In 30 day old roots, the activity is increased two to three times by an anoxic or hypoxic treatment and can be detected on immunoblots by an antiserum raised against barley lactate dehydrogenase. The activity present in aerobic seedlings was partially purified. The native enzyme has a molecular mass of 160 kilodaltons, and is a tetramer of 2 subunit (38 and 39 kilodaltons) randomly associated. Studies of substrate specificity, native gel electrophoresis, and immunoblot analysis indicate that the partially purified enzyme is a typical lactate dehydrogenase. However, no increase of lactate dehydrogenase activity or protein was observed in seedlings transferred to anoxia</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.95.3.682</identifier><identifier>PMID: 16668039</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ANAEROBIOSE ; ANAEROBIOSIS ; Barley ; Biological and medical sciences ; CONTENIDO PROTEICO ; Dehydrogenases ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Gels ; Genetics ; Hypoxia ; LACTATE DESHYDROGENASE ; Lactates ; LACTATO DESHIDROGENASA ; Life Sciences ; Membranes and Bioenergetics ; Metabolism ; ORYZA SATIVA ; OXIGENO ; OXYGENE ; Plant physiology and development ; Plant roots ; Plants ; Plants genetics ; PLANTULAS ; PLANTULE ; RACINE ; RAICES ; Rice ; Seedlings ; TENEUR EN PROTEINES</subject><ispartof>Plant physiology (Bethesda), 1991-03, Vol.95 (3), p.682-686</ispartof><rights>Copyright 1991 American Society of Plant Physiologists</rights><rights>1991 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4273445$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4273445$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19813171$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16668039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02707360$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI)</creatorcontrib><creatorcontrib>Ricard, B</creatorcontrib><creatorcontrib>Pradet, A</creatorcontrib><title>Lactate dehydrogenase in Oryza sativa L. seedlings and roots. Identification and partial characterization</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots. Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots. In 30 day old roots, the activity is increased two to three times by an anoxic or hypoxic treatment and can be detected on immunoblots by an antiserum raised against barley lactate dehydrogenase. The activity present in aerobic seedlings was partially purified. The native enzyme has a molecular mass of 160 kilodaltons, and is a tetramer of 2 subunit (38 and 39 kilodaltons) randomly associated. Studies of substrate specificity, native gel electrophoresis, and immunoblot analysis indicate that the partially purified enzyme is a typical lactate dehydrogenase. However, no increase of lactate dehydrogenase activity or protein was observed in seedlings transferred to anoxia</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ANAEROBIOSE</subject><subject>ANAEROBIOSIS</subject><subject>Barley</subject><subject>Biological and medical sciences</subject><subject>CONTENIDO PROTEICO</subject><subject>Dehydrogenases</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genetics</subject><subject>Hypoxia</subject><subject>LACTATE DESHYDROGENASE</subject><subject>Lactates</subject><subject>LACTATO DESHIDROGENASA</subject><subject>Life Sciences</subject><subject>Membranes and Bioenergetics</subject><subject>Metabolism</subject><subject>ORYZA SATIVA</subject><subject>OXIGENO</subject><subject>OXYGENE</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>Plants</subject><subject>Plants genetics</subject><subject>PLANTULAS</subject><subject>PLANTULE</subject><subject>RACINE</subject><subject>RAICES</subject><subject>Rice</subject><subject>Seedlings</subject><subject>TENEUR EN PROTEINES</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9ks9v0zAUxyPExMrgxA0hlAsghBrs-PcFaZpgmxRpB9jZerWd1lMaZ3ZaqfvrcZtqAw6cbL_vx9_3rK-L4g1GFcaIfh2GSrGKVFzWz4oZZqSe14zK58UMobxHUqrT4mVKdwghTDB9UZxizrlERM0K34AZYXSldaudjWHpekiu9H15E3cPUCYY_RbKpiqTc7bz_TKV0NsyhjCmqry2rh99603GQn9QBoijh640K4jZ20X_cBBfFSctdMm9Pq5nxe2P778urubNzeX1xXkzN5SrcV5bjlvcIpA1MyCsNJYztHALYiljzAhqgCtDGMNsQRRZEOewkZQ4gfOpJmfFt8l32CzWzpo8YIROD9GvIe50AK__Vnq_0suw1RgJwRTOBp8ng9U_167OG72voVogQTja7tlPx2Yx3G9cGvXaJ-O6DnoXNkkLQqiimNNMfvwviTnKoR3af5lAE0NK0bWPM2Ck94HrYdCKaaJz4Jl-_-drn9hjwhn4cAQgGejaCL3x6YlTMv8JsW_7buLu0hjio05rQShlWX47yS0EDcuYLW5_KlxTwST5DS17xd0</recordid><startdate>19910301</startdate><enddate>19910301</enddate><creator>Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI)</creator><creator>Ricard, B</creator><creator>Pradet, A</creator><general>American Society of Plant Physiologists</general><general>Oxford University Press ; American Society of Plant Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>19910301</creationdate><title>Lactate dehydrogenase in Oryza sativa L. seedlings and roots. Identification and partial characterization</title><author>Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI) ; Ricard, B ; Pradet, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-2d61f1f0a825ca7d8cd650beb3d4555c74ca69c35515b393b3ee1c843e7193b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ANAEROBIOSE</topic><topic>ANAEROBIOSIS</topic><topic>Barley</topic><topic>Biological and medical sciences</topic><topic>CONTENIDO PROTEICO</topic><topic>Dehydrogenases</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genetics</topic><topic>Hypoxia</topic><topic>LACTATE DESHYDROGENASE</topic><topic>Lactates</topic><topic>LACTATO DESHIDROGENASA</topic><topic>Life Sciences</topic><topic>Membranes and Bioenergetics</topic><topic>Metabolism</topic><topic>ORYZA SATIVA</topic><topic>OXIGENO</topic><topic>OXYGENE</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>Plants</topic><topic>Plants genetics</topic><topic>PLANTULAS</topic><topic>PLANTULE</topic><topic>RACINE</topic><topic>RAICES</topic><topic>Rice</topic><topic>Seedlings</topic><topic>TENEUR EN PROTEINES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI)</creatorcontrib><creatorcontrib>Ricard, B</creatorcontrib><creatorcontrib>Pradet, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rivoal, J. (MSU-DOE Plant Research Laboratory, East Lansing, MI)</au><au>Ricard, B</au><au>Pradet, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactate dehydrogenase in Oryza sativa L. seedlings and roots. Identification and partial characterization</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1991-03-01</date><risdate>1991</risdate><volume>95</volume><issue>3</issue><spage>682</spage><epage>686</epage><pages>682-686</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A lactate dehydrogenase activity is present in rice (Oryza sativa L.) seedlings and roots. Under aerobic conditions, lactate dehydrogenase activity is barely detectable in rice seedlings and is very low in rice roots. In 30 day old roots, the activity is increased two to three times by an anoxic or hypoxic treatment and can be detected on immunoblots by an antiserum raised against barley lactate dehydrogenase. The activity present in aerobic seedlings was partially purified. The native enzyme has a molecular mass of 160 kilodaltons, and is a tetramer of 2 subunit (38 and 39 kilodaltons) randomly associated. Studies of substrate specificity, native gel electrophoresis, and immunoblot analysis indicate that the partially purified enzyme is a typical lactate dehydrogenase. However, no increase of lactate dehydrogenase activity or protein was observed in seedlings transferred to anoxia</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16668039</pmid><doi>10.1104/pp.95.3.682</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Jstor Complete Legacy; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ANAEROBIOSE ANAEROBIOSIS Barley Biological and medical sciences CONTENIDO PROTEICO Dehydrogenases Enzymes Fundamental and applied biological sciences. Psychology Gels Genetics Hypoxia LACTATE DESHYDROGENASE Lactates LACTATO DESHIDROGENASA Life Sciences Membranes and Bioenergetics Metabolism ORYZA SATIVA OXIGENO OXYGENE Plant physiology and development Plant roots Plants Plants genetics PLANTULAS PLANTULE RACINE RAICES Rice Seedlings TENEUR EN PROTEINES |
| title | Lactate dehydrogenase in Oryza sativa L. seedlings and roots. Identification and partial characterization |
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