High levels of tryptamine accumulation in transgenic tobacco expressing tryptophan decarboxylase
A full-length complementary DNA clone encoding tryptophan decarboxylase (TDC; EC 4.1.1.28) from Catharanthus roseus (De Luca V, Marineou C, Brisson N [1989] Proc Nad Acad Sci USA 86: 2582-2586) driven by the CaMV 35S promoter was introduced into tobacco (Nicotiana tabacum) to direct the synthesis of...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1990-11, Vol.94 (3), p.1410-1413 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Songstad, D.D. (Monsanto Company, Chesterfield, MO) De Luca, V Brisson, N Kurz, W.G.W Nessier, C.L |
| description | A full-length complementary DNA clone encoding tryptophan decarboxylase (TDC; EC 4.1.1.28) from Catharanthus roseus (De Luca V, Marineou C, Brisson N [1989] Proc Nad Acad Sci USA 86: 2582-2586) driven by the CaMV 35S promoter was introduced into tobacco (Nicotiana tabacum) to direct the synthesis of the protoalkaloid tryptamine from endogenous tryptophan. Young, fully expanded leaves of CaMV 35S-TDC transformed plants had from four to 45 times greater TDC activity than did controls. Tryptamine accumulated in transgenic plants to levels that were directly proportional to their TDC specific activity. Despite their increased tryptamine content, the growth and development of the CaMV 35S-TDC plants appeared normal with no significant differences in indole-3-acetic acid levels between high tryptamine and control plants. Plants with the highest TDC activity contained more than 1 milligram of tryptamine per gram fresh weight, a 260-fold increase over controls |
| doi_str_mv | 10.1104/pp.94.3.1410 |
| format | Article |
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(Monsanto Company, Chesterfield, MO) ; De Luca, V ; Brisson, N ; Kurz, W.G.W ; Nessier, C.L</creator><creatorcontrib>Songstad, D.D. (Monsanto Company, Chesterfield, MO) ; De Luca, V ; Brisson, N ; Kurz, W.G.W ; Nessier, C.L</creatorcontrib><description>A full-length complementary DNA clone encoding tryptophan decarboxylase (TDC; EC 4.1.1.28) from Catharanthus roseus (De Luca V, Marineou C, Brisson N [1989] Proc Nad Acad Sci USA 86: 2582-2586) driven by the CaMV 35S promoter was introduced into tobacco (Nicotiana tabacum) to direct the synthesis of the protoalkaloid tryptamine from endogenous tryptophan. Young, fully expanded leaves of CaMV 35S-TDC transformed plants had from four to 45 times greater TDC activity than did controls. Tryptamine accumulated in transgenic plants to levels that were directly proportional to their TDC specific activity. Despite their increased tryptamine content, the growth and development of the CaMV 35S-TDC plants appeared normal with no significant differences in indole-3-acetic acid levels between high tryptamine and control plants. Plants with the highest TDC activity contained more than 1 milligram of tryptamine per gram fresh weight, a 260-fold increase over controls</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.94.3.1410</identifier><identifier>PMID: 16667846</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; AGROBACTERIUM TUMEFACIENS ; Alkaloids ; Auxins ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; Biosynthesis ; Biotechnology ; Complementary DNA ; DNA ; EXPRESION GENICA ; EXPRESSION DES GENES ; Flower buds ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; GENETICA ; GENETIQUE ; Leaves ; LIASAS ; LYASE ; Methods. Procedures. 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(Monsanto Company, Chesterfield, MO)</creatorcontrib><creatorcontrib>De Luca, V</creatorcontrib><creatorcontrib>Brisson, N</creatorcontrib><creatorcontrib>Kurz, W.G.W</creatorcontrib><creatorcontrib>Nessier, C.L</creatorcontrib><title>High levels of tryptamine accumulation in transgenic tobacco expressing tryptophan decarboxylase</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A full-length complementary DNA clone encoding tryptophan decarboxylase (TDC; EC 4.1.1.28) from Catharanthus roseus (De Luca V, Marineou C, Brisson N [1989] Proc Nad Acad Sci USA 86: 2582-2586) driven by the CaMV 35S promoter was introduced into tobacco (Nicotiana tabacum) to direct the synthesis of the protoalkaloid tryptamine from endogenous tryptophan. Young, fully expanded leaves of CaMV 35S-TDC transformed plants had from four to 45 times greater TDC activity than did controls. Tryptamine accumulated in transgenic plants to levels that were directly proportional to their TDC specific activity. Despite their increased tryptamine content, the growth and development of the CaMV 35S-TDC plants appeared normal with no significant differences in indole-3-acetic acid levels between high tryptamine and control plants. Plants with the highest TDC activity contained more than 1 milligram of tryptamine per gram fresh weight, a 260-fold increase over controls</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>AGROBACTERIUM TUMEFACIENS</subject><subject>Alkaloids</subject><subject>Auxins</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Complementary DNA</subject><subject>DNA</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Flower buds</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Leaves</subject><subject>LIASAS</subject><subject>LYASE</subject><subject>Methods. Procedures. Technologies</subject><subject>Natural Products</subject><subject>NICOTIANA TABACUM</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants</subject><subject>RNA</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>TRIPTOFANO</subject><subject>TRYPTOPHANE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpVkc9rFDEcxYModq3ePInIXMSLuyaT35eCFLVCwYP2HDPZb2ZTZpMxmSnd_94ss7R6SuB98t4jD6HXBG8IwezTOG4029ANYQQ_QSvCabtuOVNP0QrjesdK6TP0opRbjDGhhD1HZ0QIIRUTK_T7KvS7ZoA7GEqTfDPlwzjZfYjQWOfm_TzYKaTYhFglG0sPMbhmSl1VUwP3Y4ZSQuyXh2nc2dhswdncpfvDYAu8RM-8HQq8Op3n6Obrl1-XV-vrH9--X36-XjuuxbT2FrDiiilKRSu09wJ7L7fWYqmp5J3jaqup5rTrlGdeAufggVJpPaPSaXqOLhbfce72sHUQa9_BjDnsbT6YZIP5X4lhZ_p0ZwiWkmpSDT6cDHL6M0OZzD4UB8NgI6S5GEkpqw0Ur-THhXQ5lZLBP6QQbI6bmHE0mhlqjptU_N2_zR7h0wgVeH8CbHF28PWfXSiPXM3kgsnKvV242zKl_KCzVtKWH3PeLLK3ydg-V4ubn5qQVlBN_wLMqKmJ</recordid><startdate>19901101</startdate><enddate>19901101</enddate><creator>Songstad, D.D. 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(Monsanto Company, Chesterfield, MO) ; De Luca, V ; Brisson, N ; Kurz, W.G.W ; Nessier, C.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-fae085848336269ff60ff7daa079375bc58d93953bb8f4f7e55efe337af437c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>AGROBACTERIUM TUMEFACIENS</topic><topic>Alkaloids</topic><topic>Auxins</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Complementary DNA</topic><topic>DNA</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Flower buds</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Leaves</topic><topic>LIASAS</topic><topic>LYASE</topic><topic>Methods. Procedures. Technologies</topic><topic>Natural Products</topic><topic>NICOTIANA TABACUM</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants</topic><topic>RNA</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>TRIPTOFANO</topic><topic>TRYPTOPHANE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Songstad, D.D. (Monsanto Company, Chesterfield, MO)</creatorcontrib><creatorcontrib>De Luca, V</creatorcontrib><creatorcontrib>Brisson, N</creatorcontrib><creatorcontrib>Kurz, W.G.W</creatorcontrib><creatorcontrib>Nessier, C.L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Songstad, D.D. (Monsanto Company, Chesterfield, MO)</au><au>De Luca, V</au><au>Brisson, N</au><au>Kurz, W.G.W</au><au>Nessier, C.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High levels of tryptamine accumulation in transgenic tobacco expressing tryptophan decarboxylase</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1990-11-01</date><risdate>1990</risdate><volume>94</volume><issue>3</issue><spage>1410</spage><epage>1413</epage><pages>1410-1413</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A full-length complementary DNA clone encoding tryptophan decarboxylase (TDC; EC 4.1.1.28) from Catharanthus roseus (De Luca V, Marineou C, Brisson N [1989] Proc Nad Acad Sci USA 86: 2582-2586) driven by the CaMV 35S promoter was introduced into tobacco (Nicotiana tabacum) to direct the synthesis of the protoalkaloid tryptamine from endogenous tryptophan. Young, fully expanded leaves of CaMV 35S-TDC transformed plants had from four to 45 times greater TDC activity than did controls. Tryptamine accumulated in transgenic plants to levels that were directly proportional to their TDC specific activity. Despite their increased tryptamine content, the growth and development of the CaMV 35S-TDC plants appeared normal with no significant differences in indole-3-acetic acid levels between high tryptamine and control plants. Plants with the highest TDC activity contained more than 1 milligram of tryptamine per gram fresh weight, a 260-fold increase over controls</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16667846</pmid><doi>10.1104/pp.94.3.1410</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1990-11, Vol.94 (3), p.1410-1413 |
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| language | eng |
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| source | Alma/SFX Local Collection; JSTOR; EZB Electronic Journals Library |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE AGROBACTERIUM TUMEFACIENS Alkaloids Auxins Biological and medical sciences BIOSINTESIS BIOSYNTHESE Biosynthesis Biotechnology Complementary DNA DNA EXPRESION GENICA EXPRESSION DES GENES Flower buds Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics GENETICA GENETIQUE Leaves LIASAS LYASE Methods. Procedures. Technologies Natural Products NICOTIANA TABACUM PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants RNA Transgenic animals and transgenic plants Transgenic plants TRIPTOFANO TRYPTOPHANE |
| title | High levels of tryptamine accumulation in transgenic tobacco expressing tryptophan decarboxylase |
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