Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves
Under progressive drought stress, Brassica napus displays differential leaf modifications. The oldest leaves, developed before the onset of water deficit, wilt gradually, whereas the youngest leaves harden. Hardening was distinguished by leaf turgor and bluish wax bloom when the shoot water potentia...
Gespeichert in:
| Veröffentlicht in: | Plant physiology (Bethesda) 1992-11, Vol.100 (3), p.1486-1493 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1493 |
|---|---|
| container_issue | 3 |
| container_start_page | 1486 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 100 |
| creator | Reviron, M.P Vartanian, N Sallantin, M Huet, J.C Pernollet, J.C Vienne, D. de |
| description | Under progressive drought stress, Brassica napus displays differential leaf modifications. The oldest leaves, developed before the onset of water deficit, wilt gradually, whereas the youngest leaves harden. Hardening was distinguished by leaf turgor and bluish wax bloom when the shoot water potential was below -3 MPa and the leaf water saturation deficit was about 60%. This adaptive change was accompanied by modifications in two-dimensional protein profiles. Ten percent of the polypeptides had altered abundance or were unique to drought-stressed plants. Two-dimensional analysis of in vitro translation products did not reveal a general decrease in mRNA population. A 22-kD double polypeptide was increased by progressive or rapid water stress and salinity and disappeared upon rehydration. These polypeptides have a common N-terminal sequence, which does not reveal homology with any known water-stress protein but which contains the signature motif of soybean Kunitz trypsin inhibitors. Immunoprecipitation allowed these polypeptides to be identified on two-dimensional gels of in vitro translation products. They appeared to be synthesized as a 24-kD precursor, and their transcript was present in the control well-watered leaves, where the polypeptides were never detected, indicating a possible translational regulation. A putative function of this protein, named BnD22, in the retardation of drought-induced leaf senescence is discussed |
| doi_str_mv | 10.1104/pp.100.3.1486 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1075810</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4274814</jstor_id><sourcerecordid>4274814</sourcerecordid><originalsourceid>FETCH-LOGICAL-c524t-a288990339bfdb3c9066e66eda643a3e9066c2a5b2cda2736d30feb19bbb6d1c3</originalsourceid><addsrcrecordid>eNpdkcGL1DAUxoso7rh69CIiOQjioeNL0qbtZWEd1BUGPOiew2uSzmTpJN2kLYx_vSkz7KoQyJf3_d6XhJdlrymsKYXi0zCsKcCar2lRiyfZipac5aws6qfZCiBpqOvmInsR4x0AUE6L59kFFaJMql5l95s9BlSjCfY3jtY74juCxPnZ9GQIfjTWEev0pIwm7XEp7YKJ0c6G-EACDlYTHfy0248EnSYRe-vseExN5HPARKoUh8MUSW9wNvFl9qzDPppX5_0yu_365dfmJt_--PZ9c73NVcmKMUeW3t0A503b6ZarBoQwaWkUBUdulrNiWLZMaWQVF5pDZ1ratG0rNFX8Mrs65Q5TezBaGTcG7OUQ7AHDUXq08l_H2b3c-VlSqMqaQgr4eArY_9d2c72VSw1YRVkF9UwT--F8WfD3k4mjPNioTN-jM36KsuK8qDlQlsj8RKrgYwyme4imIJeJymFIEiSXy0QT_-7vbzzS5xEm4P0ZwKiw7wI6ZeMDVwigolpy3p6wuzj68Gizqqhpkew3J7tDL3EXUsLtzya5AA3_AzxCvK0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733483012</pqid></control><display><type>article</type><title>Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves</title><source>Jstor Complete Legacy</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Reviron, M.P ; Vartanian, N ; Sallantin, M ; Huet, J.C ; Pernollet, J.C ; Vienne, D. de</creator><creatorcontrib>Reviron, M.P ; Vartanian, N ; Sallantin, M ; Huet, J.C ; Pernollet, J.C ; Vienne, D. de</creatorcontrib><description>Under progressive drought stress, Brassica napus displays differential leaf modifications. The oldest leaves, developed before the onset of water deficit, wilt gradually, whereas the youngest leaves harden. Hardening was distinguished by leaf turgor and bluish wax bloom when the shoot water potential was below -3 MPa and the leaf water saturation deficit was about 60%. This adaptive change was accompanied by modifications in two-dimensional protein profiles. Ten percent of the polypeptides had altered abundance or were unique to drought-stressed plants. Two-dimensional analysis of in vitro translation products did not reveal a general decrease in mRNA population. A 22-kD double polypeptide was increased by progressive or rapid water stress and salinity and disappeared upon rehydration. These polypeptides have a common N-terminal sequence, which does not reveal homology with any known water-stress protein but which contains the signature motif of soybean Kunitz trypsin inhibitors. Immunoprecipitation allowed these polypeptides to be identified on two-dimensional gels of in vitro translation products. They appeared to be synthesized as a 24-kD precursor, and their transcript was present in the control well-watered leaves, where the polypeptides were never detected, indicating a possible translational regulation. A putative function of this protein, named BnD22, in the retardation of drought-induced leaf senescence is discussed</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.100.3.1486</identifier><identifier>PMID: 16653148</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ADAPTACION ; ADAPTATION ; ARN MENSAJERO ; ARN MESSAGER ; BALANCE HIDRICO DEL SUELO ; BILAN HYDRIQUE DU SOL ; Biological and medical sciences ; BRASSICA NAPUS ; Canola ; Dehydration ; Drought ; Environmental and Stress Physiology ; ESTRES DE SEQUIA ; EXPRESION GENICA ; EXPRESSION DES GENES ; FEUILLE ; Fundamental and applied biological sciences. Psychology ; Gels ; Genetics ; HOJAS ; Leaves ; Life Sciences ; Oral rehydration ; Plant physiology and development ; Plants ; Plants genetics ; PROTEINAS VEGETALES ; PROTEINE VEGETALE ; Proteins ; SALINIDAD ; SALINITE ; Salinity ; SECHERESSE ; SEQUIA ; STRESS DU A LA SECHERESSE ; Turgor pressure ; Water and solutes. Absorption, translocation and permeability</subject><ispartof>Plant physiology (Bethesda), 1992-11, Vol.100 (3), p.1486-1493</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-a288990339bfdb3c9066e66eda643a3e9066c2a5b2cda2736d30feb19bbb6d1c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4274814$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4274814$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4601676$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16653148$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02712708$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Reviron, M.P</creatorcontrib><creatorcontrib>Vartanian, N</creatorcontrib><creatorcontrib>Sallantin, M</creatorcontrib><creatorcontrib>Huet, J.C</creatorcontrib><creatorcontrib>Pernollet, J.C</creatorcontrib><creatorcontrib>Vienne, D. de</creatorcontrib><title>Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Under progressive drought stress, Brassica napus displays differential leaf modifications. The oldest leaves, developed before the onset of water deficit, wilt gradually, whereas the youngest leaves harden. Hardening was distinguished by leaf turgor and bluish wax bloom when the shoot water potential was below -3 MPa and the leaf water saturation deficit was about 60%. This adaptive change was accompanied by modifications in two-dimensional protein profiles. Ten percent of the polypeptides had altered abundance or were unique to drought-stressed plants. Two-dimensional analysis of in vitro translation products did not reveal a general decrease in mRNA population. A 22-kD double polypeptide was increased by progressive or rapid water stress and salinity and disappeared upon rehydration. These polypeptides have a common N-terminal sequence, which does not reveal homology with any known water-stress protein but which contains the signature motif of soybean Kunitz trypsin inhibitors. Immunoprecipitation allowed these polypeptides to be identified on two-dimensional gels of in vitro translation products. They appeared to be synthesized as a 24-kD precursor, and their transcript was present in the control well-watered leaves, where the polypeptides were never detected, indicating a possible translational regulation. A putative function of this protein, named BnD22, in the retardation of drought-induced leaf senescence is discussed</description><subject>ADAPTACION</subject><subject>ADAPTATION</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>BALANCE HIDRICO DEL SUELO</subject><subject>BILAN HYDRIQUE DU SOL</subject><subject>Biological and medical sciences</subject><subject>BRASSICA NAPUS</subject><subject>Canola</subject><subject>Dehydration</subject><subject>Drought</subject><subject>Environmental and Stress Physiology</subject><subject>ESTRES DE SEQUIA</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FEUILLE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genetics</subject><subject>HOJAS</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Oral rehydration</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Plants genetics</subject><subject>PROTEINAS VEGETALES</subject><subject>PROTEINE VEGETALE</subject><subject>Proteins</subject><subject>SALINIDAD</subject><subject>SALINITE</subject><subject>Salinity</subject><subject>SECHERESSE</subject><subject>SEQUIA</subject><subject>STRESS DU A LA SECHERESSE</subject><subject>Turgor pressure</subject><subject>Water and solutes. Absorption, translocation and permeability</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpdkcGL1DAUxoso7rh69CIiOQjioeNL0qbtZWEd1BUGPOiew2uSzmTpJN2kLYx_vSkz7KoQyJf3_d6XhJdlrymsKYXi0zCsKcCar2lRiyfZipac5aws6qfZCiBpqOvmInsR4x0AUE6L59kFFaJMql5l95s9BlSjCfY3jtY74juCxPnZ9GQIfjTWEev0pIwm7XEp7YKJ0c6G-EACDlYTHfy0248EnSYRe-vseExN5HPARKoUh8MUSW9wNvFl9qzDPppX5_0yu_365dfmJt_--PZ9c73NVcmKMUeW3t0A503b6ZarBoQwaWkUBUdulrNiWLZMaWQVF5pDZ1ratG0rNFX8Mrs65Q5TezBaGTcG7OUQ7AHDUXq08l_H2b3c-VlSqMqaQgr4eArY_9d2c72VSw1YRVkF9UwT--F8WfD3k4mjPNioTN-jM36KsuK8qDlQlsj8RKrgYwyme4imIJeJymFIEiSXy0QT_-7vbzzS5xEm4P0ZwKiw7wI6ZeMDVwigolpy3p6wuzj68Gizqqhpkew3J7tDL3EXUsLtzya5AA3_AzxCvK0</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Reviron, M.P</creator><creator>Vartanian, N</creator><creator>Sallantin, M</creator><creator>Huet, J.C</creator><creator>Pernollet, J.C</creator><creator>Vienne, D. de</creator><general>American Society of Plant Physiologists</general><general>Oxford University Press ; American Society of Plant Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>19921101</creationdate><title>Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves</title><author>Reviron, M.P ; Vartanian, N ; Sallantin, M ; Huet, J.C ; Pernollet, J.C ; Vienne, D. de</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-a288990339bfdb3c9066e66eda643a3e9066c2a5b2cda2736d30feb19bbb6d1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ADAPTACION</topic><topic>ADAPTATION</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>BALANCE HIDRICO DEL SUELO</topic><topic>BILAN HYDRIQUE DU SOL</topic><topic>Biological and medical sciences</topic><topic>BRASSICA NAPUS</topic><topic>Canola</topic><topic>Dehydration</topic><topic>Drought</topic><topic>Environmental and Stress Physiology</topic><topic>ESTRES DE SEQUIA</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FEUILLE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genetics</topic><topic>HOJAS</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Oral rehydration</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Plants genetics</topic><topic>PROTEINAS VEGETALES</topic><topic>PROTEINE VEGETALE</topic><topic>Proteins</topic><topic>SALINIDAD</topic><topic>SALINITE</topic><topic>Salinity</topic><topic>SECHERESSE</topic><topic>SEQUIA</topic><topic>STRESS DU A LA SECHERESSE</topic><topic>Turgor pressure</topic><topic>Water and solutes. Absorption, translocation and permeability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reviron, M.P</creatorcontrib><creatorcontrib>Vartanian, N</creatorcontrib><creatorcontrib>Sallantin, M</creatorcontrib><creatorcontrib>Huet, J.C</creatorcontrib><creatorcontrib>Pernollet, J.C</creatorcontrib><creatorcontrib>Vienne, D. de</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reviron, M.P</au><au>Vartanian, N</au><au>Sallantin, M</au><au>Huet, J.C</au><au>Pernollet, J.C</au><au>Vienne, D. de</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>100</volume><issue>3</issue><spage>1486</spage><epage>1493</epage><pages>1486-1493</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Under progressive drought stress, Brassica napus displays differential leaf modifications. The oldest leaves, developed before the onset of water deficit, wilt gradually, whereas the youngest leaves harden. Hardening was distinguished by leaf turgor and bluish wax bloom when the shoot water potential was below -3 MPa and the leaf water saturation deficit was about 60%. This adaptive change was accompanied by modifications in two-dimensional protein profiles. Ten percent of the polypeptides had altered abundance or were unique to drought-stressed plants. Two-dimensional analysis of in vitro translation products did not reveal a general decrease in mRNA population. A 22-kD double polypeptide was increased by progressive or rapid water stress and salinity and disappeared upon rehydration. These polypeptides have a common N-terminal sequence, which does not reveal homology with any known water-stress protein but which contains the signature motif of soybean Kunitz trypsin inhibitors. Immunoprecipitation allowed these polypeptides to be identified on two-dimensional gels of in vitro translation products. They appeared to be synthesized as a 24-kD precursor, and their transcript was present in the control well-watered leaves, where the polypeptides were never detected, indicating a possible translational regulation. A putative function of this protein, named BnD22, in the retardation of drought-induced leaf senescence is discussed</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16653148</pmid><doi>10.1104/pp.100.3.1486</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1992-11, Vol.100 (3), p.1486-1493 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1075810 |
| source | Jstor Complete Legacy; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ADAPTACION ADAPTATION ARN MENSAJERO ARN MESSAGER BALANCE HIDRICO DEL SUELO BILAN HYDRIQUE DU SOL Biological and medical sciences BRASSICA NAPUS Canola Dehydration Drought Environmental and Stress Physiology ESTRES DE SEQUIA EXPRESION GENICA EXPRESSION DES GENES FEUILLE Fundamental and applied biological sciences. Psychology Gels Genetics HOJAS Leaves Life Sciences Oral rehydration Plant physiology and development Plants Plants genetics PROTEINAS VEGETALES PROTEINE VEGETALE Proteins SALINIDAD SALINITE Salinity SECHERESSE SEQUIA STRESS DU A LA SECHERESSE Turgor pressure Water and solutes. Absorption, translocation and permeability |
| title | Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T19%3A49%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20a%20novel%20protein%20induced%20by%20progressive%20or%20rapid%20drought%20and%20salinity%20in%20Brassica%20napus%20leaves&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Reviron,%20M.P&rft.date=1992-11-01&rft.volume=100&rft.issue=3&rft.spage=1486&rft.epage=1493&rft.pages=1486-1493&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.100.3.1486&rft_dat=%3Cjstor_pubme%3E4274814%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733483012&rft_id=info:pmid/16653148&rft_jstor_id=4274814&rfr_iscdi=true |