Involvement of the oxidative burst in phytoalexin accumulation and the hypersensitive reaction
The role of the oxidative burst, transient production of activated oxygen species such as H2O2 and superoxide (O2-) in elicitation of phytoalexins and the hypersensitive reaction (HR) was investigated in white clover (Trifolium repens L.) and tobacco (Nicotiana tabacum L.). H2O2 and O2- production w...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1992-11, Vol.100 (3), p.1189-1195 |
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| creator | DEVLIN, W. S GUSTINE, D. L |
| description | The role of the oxidative burst, transient production of activated oxygen species such as H2O2 and superoxide (O2-) in elicitation of phytoalexins and the hypersensitive reaction (HR) was investigated in white clover (Trifolium repens L.) and tobacco (Nicotiana tabacum L.). H2O2 and O2- production was measured as chemiluminescence (CL) mediated by luminol, which was added to suspension-cultured white clover just before measurement in an out-of-coincidence mode scintillation counter. Maximum CL occurred between 10 and 20 min after addition of 0.4 X 10(8) colony-forming units/mL of incompatible Pseudomonas corrugata or 158 micromolar HgCl2. Autoclaved P. corrugata produced a slightly higher response. Elicitation of cells with 25 micromolar HgCl2 did not produce CL. Preincubation of plant cells in superoxide dismutase, which converts O2- to H2O2, for 2 min before addition of bacteria did not significantly increase maximum CL levels (P less than or equal to 0.05). Preincubation of plant cells with catalase for 2 min before addition of bacteria prevented the increase in CL, confirming that H2O2 is the substrate for the luminol reaction. Addition of live bacteria or HgCl2 (25 and 158 micromolar) to white clover increased levels of the phytoalexin medicarpin during a 24-h period, but addition of autoclaved bacteria did not elicit formation of medicarpin. Preincubation of plant cells with catalase, which quenched the bacteria-induced oxidative burst, did not decrease phytoalexin accumulation. Live bacteria infiltrated into Havana 44 tobacco leaf panels induced development of the HR, but autoclaved bacteria did not. Incubation of live bacteria with superoxide dismutase and catalase before infiltration into tobacco leaves did not interfere with development of the HR. Tobacco leaf panels infiltrated with up to 158 micromolar HgCl2 did not develop an HR. These results suggest that an oxidative burst consisting of and O2- does occur during these two plant defense responses. |
| doi_str_mv | 10.1104/pp.100.3.1189 |
| format | Article |
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S ; GUSTINE, D. L</creator><creatorcontrib>DEVLIN, W. S ; GUSTINE, D. L</creatorcontrib><description>The role of the oxidative burst, transient production of activated oxygen species such as H2O2 and superoxide (O2-) in elicitation of phytoalexins and the hypersensitive reaction (HR) was investigated in white clover (Trifolium repens L.) and tobacco (Nicotiana tabacum L.). H2O2 and O2- production was measured as chemiluminescence (CL) mediated by luminol, which was added to suspension-cultured white clover just before measurement in an out-of-coincidence mode scintillation counter. Maximum CL occurred between 10 and 20 min after addition of 0.4 X 10(8) colony-forming units/mL of incompatible Pseudomonas corrugata or 158 micromolar HgCl2. Autoclaved P. corrugata produced a slightly higher response. Elicitation of cells with 25 micromolar HgCl2 did not produce CL. Preincubation of plant cells in superoxide dismutase, which converts O2- to H2O2, for 2 min before addition of bacteria did not significantly increase maximum CL levels (P less than or equal to 0.05). Preincubation of plant cells with catalase for 2 min before addition of bacteria prevented the increase in CL, confirming that H2O2 is the substrate for the luminol reaction. Addition of live bacteria or HgCl2 (25 and 158 micromolar) to white clover increased levels of the phytoalexin medicarpin during a 24-h period, but addition of autoclaved bacteria did not elicit formation of medicarpin. Preincubation of plant cells with catalase, which quenched the bacteria-induced oxidative burst, did not decrease phytoalexin accumulation. Live bacteria infiltrated into Havana 44 tobacco leaf panels induced development of the HR, but autoclaved bacteria did not. Incubation of live bacteria with superoxide dismutase and catalase before infiltration into tobacco leaves did not interfere with development of the HR. Tobacco leaf panels infiltrated with up to 158 micromolar HgCl2 did not develop an HR. These results suggest that an oxidative burst consisting of and O2- does occur during these two plant defense responses.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.100.3.1189</identifier><identifier>PMID: 16653104</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Agronomy. Soil science and plant productions ; Bacteria ; Bacterial plant pathogens ; Biological and medical sciences ; biosintesis ; biosynthese ; biosynthesis ; Callus ; catalasa ; catalase ; Cell membranes ; Chemical suspensions ; chlorure mercurique ; cloruro mercurico ; Clover ; contaminantes ; Enzymes ; fitoalexina ; Fundamental and applied biological sciences. Psychology ; hydrogen peroxide ; Hypersensitive response ; mercuric chloride ; Microbe-Plant Interactions ; nicotiana tabacum ; optical properties ; oxigeno ; Oxygen ; oxygene ; Pathology. Damages, economic importance ; peroxido de hidrogeno ; peroxyde d' hydrogene ; phytoalexine ; phytoalexins ; Phytopathology. Animal pests. Plant and forest protection ; Plant cells ; polluant ; pollutants ; propiedades opticas ; propriete optique ; pseudomonas ; Reactive oxygen species ; superoxide dismutase ; superoxido dismutasa ; superoxyde dismutase ; trifolium repens</subject><ispartof>Plant physiology (Bethesda), 1992-11, Vol.100 (3), p.1189-1195</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-680b029bbf74f90c799ab070c0b41d69bad8b7083cdf3dc29fb34b498fd291433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4274769$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4274769$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4601704$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16653104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DEVLIN, W. S</creatorcontrib><creatorcontrib>GUSTINE, D. L</creatorcontrib><title>Involvement of the oxidative burst in phytoalexin accumulation and the hypersensitive reaction</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The role of the oxidative burst, transient production of activated oxygen species such as H2O2 and superoxide (O2-) in elicitation of phytoalexins and the hypersensitive reaction (HR) was investigated in white clover (Trifolium repens L.) and tobacco (Nicotiana tabacum L.). H2O2 and O2- production was measured as chemiluminescence (CL) mediated by luminol, which was added to suspension-cultured white clover just before measurement in an out-of-coincidence mode scintillation counter. Maximum CL occurred between 10 and 20 min after addition of 0.4 X 10(8) colony-forming units/mL of incompatible Pseudomonas corrugata or 158 micromolar HgCl2. Autoclaved P. corrugata produced a slightly higher response. Elicitation of cells with 25 micromolar HgCl2 did not produce CL. Preincubation of plant cells in superoxide dismutase, which converts O2- to H2O2, for 2 min before addition of bacteria did not significantly increase maximum CL levels (P less than or equal to 0.05). Preincubation of plant cells with catalase for 2 min before addition of bacteria prevented the increase in CL, confirming that H2O2 is the substrate for the luminol reaction. Addition of live bacteria or HgCl2 (25 and 158 micromolar) to white clover increased levels of the phytoalexin medicarpin during a 24-h period, but addition of autoclaved bacteria did not elicit formation of medicarpin. Preincubation of plant cells with catalase, which quenched the bacteria-induced oxidative burst, did not decrease phytoalexin accumulation. Live bacteria infiltrated into Havana 44 tobacco leaf panels induced development of the HR, but autoclaved bacteria did not. Incubation of live bacteria with superoxide dismutase and catalase before infiltration into tobacco leaves did not interfere with development of the HR. Tobacco leaf panels infiltrated with up to 158 micromolar HgCl2 did not develop an HR. These results suggest that an oxidative burst consisting of and O2- does occur during these two plant defense responses.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Bacteria</subject><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>biosintesis</subject><subject>biosynthese</subject><subject>biosynthesis</subject><subject>Callus</subject><subject>catalasa</subject><subject>catalase</subject><subject>Cell membranes</subject><subject>Chemical suspensions</subject><subject>chlorure mercurique</subject><subject>cloruro mercurico</subject><subject>Clover</subject><subject>contaminantes</subject><subject>Enzymes</subject><subject>fitoalexina</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hydrogen peroxide</subject><subject>Hypersensitive response</subject><subject>mercuric chloride</subject><subject>Microbe-Plant Interactions</subject><subject>nicotiana tabacum</subject><subject>optical properties</subject><subject>oxigeno</subject><subject>Oxygen</subject><subject>oxygene</subject><subject>Pathology. Damages, economic importance</subject><subject>peroxido de hidrogeno</subject><subject>peroxyde d' hydrogene</subject><subject>phytoalexine</subject><subject>phytoalexins</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant cells</subject><subject>polluant</subject><subject>pollutants</subject><subject>propiedades opticas</subject><subject>propriete optique</subject><subject>pseudomonas</subject><subject>Reactive oxygen species</subject><subject>superoxide dismutase</subject><subject>superoxido dismutasa</subject><subject>superoxyde dismutase</subject><subject>trifolium repens</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpVkc1v3CAQxVHUKtluc-ytinyI1JO3g8EGLpWiqB-RIvXQ5hoEGLJEtnHAXmX_-7IfStITb_R-MwM8hD5hWGEM9Os4rjDAiuSKixO0wDWpyqqm_B1aAGQNnIsz9CGlRwDABNNTdIabpia5e4Hub4ZN6Da2t8NUBFdMa1uEZ9-qyW9soeeYpsIPxbjeTkF19jlrZczcz10mQi6Gdt-z3o42Jjskv2-MVpmd_xG9d6pL9vx4LtHdj-9_r3-Vt79_3lxf3ZambqqpbDhoqITWjlEnwDAhlAYGBjTFbSO0arlmwIlpHWlNJZwmVFPBXVsJTAlZom-HueOse9ua_JqoOjlG36u4lUF5-b8z-LV8CBuJgdUsf8YSfTkOiOFptmmSvU_Gdp0abJiTZIRQXgHZkeWBNDGkFK172YJB7iKR45glSCJ3kWT-4u3VXuljBhm4PAIqGdW5qAbj0wtHG8Bsj30-YI9pCvHVrhhlzZs1TgWpHmKecPcHC0EBg2gYkH_NQale</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>DEVLIN, W. S</creator><creator>GUSTINE, D. L</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19921101</creationdate><title>Involvement of the oxidative burst in phytoalexin accumulation and the hypersensitive reaction</title><author>DEVLIN, W. S ; GUSTINE, D. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-680b029bbf74f90c799ab070c0b41d69bad8b7083cdf3dc29fb34b498fd291433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Bacteria</topic><topic>Bacterial plant pathogens</topic><topic>Biological and medical sciences</topic><topic>biosintesis</topic><topic>biosynthese</topic><topic>biosynthesis</topic><topic>Callus</topic><topic>catalasa</topic><topic>catalase</topic><topic>Cell membranes</topic><topic>Chemical suspensions</topic><topic>chlorure mercurique</topic><topic>cloruro mercurico</topic><topic>Clover</topic><topic>contaminantes</topic><topic>Enzymes</topic><topic>fitoalexina</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hydrogen peroxide</topic><topic>Hypersensitive response</topic><topic>mercuric chloride</topic><topic>Microbe-Plant Interactions</topic><topic>nicotiana tabacum</topic><topic>optical properties</topic><topic>oxigeno</topic><topic>Oxygen</topic><topic>oxygene</topic><topic>Pathology. Damages, economic importance</topic><topic>peroxido de hidrogeno</topic><topic>peroxyde d' hydrogene</topic><topic>phytoalexine</topic><topic>phytoalexins</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant cells</topic><topic>polluant</topic><topic>pollutants</topic><topic>propiedades opticas</topic><topic>propriete optique</topic><topic>pseudomonas</topic><topic>Reactive oxygen species</topic><topic>superoxide dismutase</topic><topic>superoxido dismutasa</topic><topic>superoxyde dismutase</topic><topic>trifolium repens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DEVLIN, W. S</creatorcontrib><creatorcontrib>GUSTINE, D. L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DEVLIN, W. S</au><au>GUSTINE, D. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of the oxidative burst in phytoalexin accumulation and the hypersensitive reaction</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>100</volume><issue>3</issue><spage>1189</spage><epage>1195</epage><pages>1189-1195</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The role of the oxidative burst, transient production of activated oxygen species such as H2O2 and superoxide (O2-) in elicitation of phytoalexins and the hypersensitive reaction (HR) was investigated in white clover (Trifolium repens L.) and tobacco (Nicotiana tabacum L.). H2O2 and O2- production was measured as chemiluminescence (CL) mediated by luminol, which was added to suspension-cultured white clover just before measurement in an out-of-coincidence mode scintillation counter. Maximum CL occurred between 10 and 20 min after addition of 0.4 X 10(8) colony-forming units/mL of incompatible Pseudomonas corrugata or 158 micromolar HgCl2. Autoclaved P. corrugata produced a slightly higher response. Elicitation of cells with 25 micromolar HgCl2 did not produce CL. Preincubation of plant cells in superoxide dismutase, which converts O2- to H2O2, for 2 min before addition of bacteria did not significantly increase maximum CL levels (P less than or equal to 0.05). Preincubation of plant cells with catalase for 2 min before addition of bacteria prevented the increase in CL, confirming that H2O2 is the substrate for the luminol reaction. Addition of live bacteria or HgCl2 (25 and 158 micromolar) to white clover increased levels of the phytoalexin medicarpin during a 24-h period, but addition of autoclaved bacteria did not elicit formation of medicarpin. Preincubation of plant cells with catalase, which quenched the bacteria-induced oxidative burst, did not decrease phytoalexin accumulation. Live bacteria infiltrated into Havana 44 tobacco leaf panels induced development of the HR, but autoclaved bacteria did not. Incubation of live bacteria with superoxide dismutase and catalase before infiltration into tobacco leaves did not interfere with development of the HR. Tobacco leaf panels infiltrated with up to 158 micromolar HgCl2 did not develop an HR. These results suggest that an oxidative burst consisting of and O2- does occur during these two plant defense responses.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16653104</pmid><doi>10.1104/pp.100.3.1189</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Bacteria Bacterial plant pathogens Biological and medical sciences biosintesis biosynthese biosynthesis Callus catalasa catalase Cell membranes Chemical suspensions chlorure mercurique cloruro mercurico Clover contaminantes Enzymes fitoalexina Fundamental and applied biological sciences. Psychology hydrogen peroxide Hypersensitive response mercuric chloride Microbe-Plant Interactions nicotiana tabacum optical properties oxigeno Oxygen oxygene Pathology. Damages, economic importance peroxido de hidrogeno peroxyde d' hydrogene phytoalexine phytoalexins Phytopathology. Animal pests. Plant and forest protection Plant cells polluant pollutants propiedades opticas propriete optique pseudomonas Reactive oxygen species superoxide dismutase superoxido dismutasa superoxyde dismutase trifolium repens |
| title | Involvement of the oxidative burst in phytoalexin accumulation and the hypersensitive reaction |
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