Seed effects on gibberellin metabolism in pea pericarp
Pea fruit (Pisum sativum L.) is a model system for studying the effect of seeds on fruit growth in order to understand coordination of organ development. The metabolism of 14C-labeled gibberellin A12 (Ga12) by pea pericarp was followed using a method that allows access to the seeds while maintaining...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1992-09, Vol.100 (1), p.88-94 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Ozga, J.A Brenner, M.L Reinecke, D.M |
| description | Pea fruit (Pisum sativum L.) is a model system for studying the effect of seeds on fruit growth in order to understand coordination of organ development. The metabolism of 14C-labeled gibberellin A12 (Ga12) by pea pericarp was followed using a method that allows access to the seeds while maintaining pericarp growth in situ. Identification and quantitation of GAs in pea pericarp was accomplished by combined gas chromatography-mass spectrometry following extensive purification of the putative GAs. Here we report for the first time that the metabolism of [14C]GA12 to [14C]GA19 and [14C]GA20 occurs in pericarp of seeded pea fruit. Removal of seeds from the pericarp inhibited the conversion of radiolabeled GA19 to GA20 and caused the accumulation of radiolabeled and endogenous GA19. Deseeded pericarp contained no detectable GA20, GA1, or GA8, whereas pericarp with seeds contained endogenous and radiolabeled GA20 and endogenous GA1. These data strongly suggest that seeds are required for normal GA biosynthesis in the pericarp, specifically the conversion of GA19 to GA20 |
| doi_str_mv | 10.1104/pp.100.1.88 |
| format | Article |
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The metabolism of 14C-labeled gibberellin A12 (Ga12) by pea pericarp was followed using a method that allows access to the seeds while maintaining pericarp growth in situ. Identification and quantitation of GAs in pea pericarp was accomplished by combined gas chromatography-mass spectrometry following extensive purification of the putative GAs. Here we report for the first time that the metabolism of [14C]GA12 to [14C]GA19 and [14C]GA20 occurs in pericarp of seeded pea fruit. Removal of seeds from the pericarp inhibited the conversion of radiolabeled GA19 to GA20 and caused the accumulation of radiolabeled and endogenous GA19. Deseeded pericarp contained no detectable GA20, GA1, or GA8, whereas pericarp with seeds contained endogenous and radiolabeled GA20 and endogenous GA1. These data strongly suggest that seeds are required for normal GA biosynthesis in the pericarp, specifically the conversion of GA19 to GA20</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.100.1.88</identifier><identifier>PMID: 16653006</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACIDO GIBERELICO ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biosynthesis ; Development and Growth Regulation ; Fruits ; Fundamental and applied biological sciences. Psychology ; GIBBERELLINE ; Gibberellins ; GRAINE ; Growth regulators ; Metabolism ; METABOLISME ; METABOLISMO ; Ovaries ; Peas ; Pericarp ; PERICARPE ; PERICARPIO ; PISUM SATIVUM ; Plant physiology and development ; Plants ; Radioactive decay ; Seeds ; SEMILLA</subject><ispartof>Plant physiology (Bethesda), 1992-09, Vol.100 (1), p.88-94</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-6d626fde98eb56763b0e06e45649eff90167f89cce46f3e0fd87dd65c3fb9c4f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4274597$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4274597$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27915,27916,58008,58241</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4535304$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16653006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozga, J.A</creatorcontrib><creatorcontrib>Brenner, M.L</creatorcontrib><creatorcontrib>Reinecke, D.M</creatorcontrib><title>Seed effects on gibberellin metabolism in pea pericarp</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Pea fruit (Pisum sativum L.) is a model system for studying the effect of seeds on fruit growth in order to understand coordination of organ development. The metabolism of 14C-labeled gibberellin A12 (Ga12) by pea pericarp was followed using a method that allows access to the seeds while maintaining pericarp growth in situ. Identification and quantitation of GAs in pea pericarp was accomplished by combined gas chromatography-mass spectrometry following extensive purification of the putative GAs. Here we report for the first time that the metabolism of [14C]GA12 to [14C]GA19 and [14C]GA20 occurs in pericarp of seeded pea fruit. Removal of seeds from the pericarp inhibited the conversion of radiolabeled GA19 to GA20 and caused the accumulation of radiolabeled and endogenous GA19. Deseeded pericarp contained no detectable GA20, GA1, or GA8, whereas pericarp with seeds contained endogenous and radiolabeled GA20 and endogenous GA1. These data strongly suggest that seeds are required for normal GA biosynthesis in the pericarp, specifically the conversion of GA19 to GA20</description><subject>ACIDO GIBERELICO</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Development and Growth Regulation</subject><subject>Fruits</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GIBBERELLINE</subject><subject>Gibberellins</subject><subject>GRAINE</subject><subject>Growth regulators</subject><subject>Metabolism</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>Ovaries</subject><subject>Peas</subject><subject>Pericarp</subject><subject>PERICARPE</subject><subject>PERICARPIO</subject><subject>PISUM SATIVUM</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Radioactive decay</subject><subject>Seeds</subject><subject>SEMILLA</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpVkM1r3DAQxUVoabZpT7mVUHwo5FB2O7I-LF8KJSRpIdBDmrOQ5dFWwbYcyRvof98JuyTtQeiJ9-PN0zB2ymHDOcgv87zhQHpjzBFbcSXqda2kecVWAKTBmPaYvS3lHgC44PINO-ZaKwGgV0zfIvYVhoB-KVWaqm3sOsw4DHGqRlxcl4ZYxopeMzo6OXqX53fsdXBDwfeH-4TdXV3-uvi-vvl5_ePi283aS1Mva93rWoceW4Od0o0WHSBolErLlma2wHUTTOs9Sh0EQuhN0_daeRG61ssgTtjXfe6860bsPU5LdoOdcxxd_mOTi_Z_Z4q_7TY9Wg6NUjWngPNDQE4POyyLHWPx9D03YdoV2whBTbVoiPy8J31OpWQMz1M42KdF23kmSdoaQ_THf4u9sIfNEvDpALji3RCym3wsz5xUgjhJ2Nkeuy9Lyi923UjVPpX6sLeDS9ZtMyXc3bbkGtWIvz_wl_k</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Ozga, J.A</creator><creator>Brenner, M.L</creator><creator>Reinecke, D.M</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920901</creationdate><title>Seed effects on gibberellin metabolism in pea pericarp</title><author>Ozga, J.A ; Brenner, M.L ; Reinecke, D.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-6d626fde98eb56763b0e06e45649eff90167f89cce46f3e0fd87dd65c3fb9c4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACIDO GIBERELICO</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Development and Growth Regulation</topic><topic>Fruits</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GIBBERELLINE</topic><topic>Gibberellins</topic><topic>GRAINE</topic><topic>Growth regulators</topic><topic>Metabolism</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>Ovaries</topic><topic>Peas</topic><topic>Pericarp</topic><topic>PERICARPE</topic><topic>PERICARPIO</topic><topic>PISUM SATIVUM</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Radioactive decay</topic><topic>Seeds</topic><topic>SEMILLA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozga, J.A</creatorcontrib><creatorcontrib>Brenner, M.L</creatorcontrib><creatorcontrib>Reinecke, D.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozga, J.A</au><au>Brenner, M.L</au><au>Reinecke, D.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seed effects on gibberellin metabolism in pea pericarp</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>100</volume><issue>1</issue><spage>88</spage><epage>94</epage><pages>88-94</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Pea fruit (Pisum sativum L.) is a model system for studying the effect of seeds on fruit growth in order to understand coordination of organ development. The metabolism of 14C-labeled gibberellin A12 (Ga12) by pea pericarp was followed using a method that allows access to the seeds while maintaining pericarp growth in situ. Identification and quantitation of GAs in pea pericarp was accomplished by combined gas chromatography-mass spectrometry following extensive purification of the putative GAs. Here we report for the first time that the metabolism of [14C]GA12 to [14C]GA19 and [14C]GA20 occurs in pericarp of seeded pea fruit. Removal of seeds from the pericarp inhibited the conversion of radiolabeled GA19 to GA20 and caused the accumulation of radiolabeled and endogenous GA19. Deseeded pericarp contained no detectable GA20, GA1, or GA8, whereas pericarp with seeds contained endogenous and radiolabeled GA20 and endogenous GA1. These data strongly suggest that seeds are required for normal GA biosynthesis in the pericarp, specifically the conversion of GA19 to GA20</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16653006</pmid><doi>10.1104/pp.100.1.88</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1992-09, Vol.100 (1), p.88-94 |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | ACIDO GIBERELICO Agronomy. Soil science and plant productions Biological and medical sciences Biosynthesis Development and Growth Regulation Fruits Fundamental and applied biological sciences. Psychology GIBBERELLINE Gibberellins GRAINE Growth regulators Metabolism METABOLISME METABOLISMO Ovaries Peas Pericarp PERICARPE PERICARPIO PISUM SATIVUM Plant physiology and development Plants Radioactive decay Seeds SEMILLA |
| title | Seed effects on gibberellin metabolism in pea pericarp |
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