Solution phase refinement of active site structure using 2D NMR and judiciously 13C-labeled cytochrome P450

The Cytochrome P450 (CYP450) superfamily has been the subject of intense research for over six decades. Here the HU227 strain of E. coli, lacking the δ-aminolevulinic acid (δ-ALA) synthase gene, was employed, along with [5-13C] δ-ALA, in the heterologous expression of P450cam harboring a prosthetic...

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Veröffentlicht in:	Journal of inorganic biochemistry 2023-04, Vol.241, p.112126-112126, Article 112126
Hauptverfasser:	Usai, Remigio, Kaluka, Daniel, Cai, Sheng, Sem, Daniel S., Kincaid, James R.
Format:	Artikel
Sprache:	eng
Schlagworte:	2D 13C-filtered NOESY-HMQC ALA synthesis Cytochrome P450 Docking procedures Heme proteins HU227 E. coli strain
Online-Zugang:	Volltext
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