TRPM8-mediated cutaneous stimulation modulates motor neuron activity during treadmill stepping in mice
Motor units are generally recruited from the smallest to the largest following the size principle, while cutaneous stimulation has the potential to affect spinal motor control. We aimed to examine the effects of stimulating transient receptor potential channel sub-family M8 (TRPM8) combined with exe...
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| Veröffentlicht in: | The journal of physiological sciences 2019-11, Vol.69 (6), p.931-938 |
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| creator | Tamura, Kotaro Sugita, Satoshi Tokunaga, Tadayuki Minegishi, Yoshihiko Ota, Noriyasu |
| description | Motor units are generally recruited from the smallest to the largest following the size principle, while cutaneous stimulation has the potential to affect spinal motor control. We aimed to examine the effects of stimulating transient receptor potential channel sub-family M8 (TRPM8) combined with exercise on the modulation of spinal motor neuron (MN) excitability. Mice were topically administrated 1.5% icilin on the hindlimbs, followed by treadmill stepping. Spinal cord sections were immunostained with antibodies against c-fos and choline acetyltransferase. Icilin stimulation did not change the number of c-fos+ MNs, but increased the average soma size of the c-fos+ MNs during low-speed treadmill stepping. Furthermore, icilin stimulation combined with stepping increased c-fos+ cholinergic interneurons near the central canal, which are thought to modulate MN excitability. These findings suggest that TRPM8-mediated cutaneous stimulation with low-load exercise promotes preferential recruitment of large MNs and is potentially useful as a new training method for rehabilitation. |
| doi_str_mv | 10.1007/s12576-019-00707-3 |
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We aimed to examine the effects of stimulating transient receptor potential channel sub-family M8 (TRPM8) combined with exercise on the modulation of spinal motor neuron (MN) excitability. Mice were topically administrated 1.5% icilin on the hindlimbs, followed by treadmill stepping. Spinal cord sections were immunostained with antibodies against c-fos and choline acetyltransferase. Icilin stimulation did not change the number of c-fos+ MNs, but increased the average soma size of the c-fos+ MNs during low-speed treadmill stepping. Furthermore, icilin stimulation combined with stepping increased c-fos+ cholinergic interneurons near the central canal, which are thought to modulate MN excitability. These findings suggest that TRPM8-mediated cutaneous stimulation with low-load exercise promotes preferential recruitment of large MNs and is potentially useful as a new training method for rehabilitation.</description><identifier>ISSN: 1880-6546</identifier><identifier>EISSN: 1880-6562</identifier><identifier>DOI: 10.1007/s12576-019-00707-3</identifier><identifier>PMID: 31482469</identifier><language>eng</language><publisher>Japan: Elsevier Inc</publisher><subject>Acetyltransferase ; Animals ; c-fos ; c-Fos protein ; Choline ; Choline O-acetyltransferase ; Excitability ; Exercise equipment ; Exercise Test ; Experiments ; Fitness equipment ; Gene Expression Regulation - drug effects ; Interneurons ; Male ; Mice ; Mice, Inbred C57BL ; Motor neuron ; Motor Neurons - physiology ; Motor task performance ; Motor units ; Muscle function ; Neuromodulation ; Neurons ; Original Paper ; Physical Conditioning, Animal ; Proto-Oncogene Proteins c-fos - genetics ; Proto-Oncogene Proteins c-fos - metabolism ; Pyrimidinones - pharmacology ; Recruitment ; Rehabilitation ; Skin - drug effects ; Somatosensory ; Spinal cord ; Transient receptor potential proteins ; TRPM Cation Channels - genetics ; TRPM Cation Channels - metabolism ; TRPM8</subject><ispartof>The journal of physiological sciences, 2019-11, Vol.69 (6), p.931-938</ispartof><rights>2019 The Author(s)</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Copyright Springer Nature B.V. 2019</rights><rights>2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.</rights><rights>The Author(s) 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c706t-fc9c919e73ef107730b4dc261994b7951fe4bd5ea4d39ed0474462ad71590dc83</citedby><cites>FETCH-LOGICAL-c706t-fc9c919e73ef107730b4dc261994b7951fe4bd5ea4d39ed0474462ad71590dc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10717255/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10717255/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31482469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tamura, Kotaro</creatorcontrib><creatorcontrib>Sugita, Satoshi</creatorcontrib><creatorcontrib>Tokunaga, Tadayuki</creatorcontrib><creatorcontrib>Minegishi, Yoshihiko</creatorcontrib><creatorcontrib>Ota, Noriyasu</creatorcontrib><title>TRPM8-mediated cutaneous stimulation modulates motor neuron activity during treadmill stepping in mice</title><title>The journal of physiological sciences</title><addtitle>J Physiol Sci</addtitle><description>Motor units are generally recruited from the smallest to the largest following the size principle, while cutaneous stimulation has the potential to affect spinal motor control. We aimed to examine the effects of stimulating transient receptor potential channel sub-family M8 (TRPM8) combined with exercise on the modulation of spinal motor neuron (MN) excitability. Mice were topically administrated 1.5% icilin on the hindlimbs, followed by treadmill stepping. Spinal cord sections were immunostained with antibodies against c-fos and choline acetyltransferase. Icilin stimulation did not change the number of c-fos+ MNs, but increased the average soma size of the c-fos+ MNs during low-speed treadmill stepping. Furthermore, icilin stimulation combined with stepping increased c-fos+ cholinergic interneurons near the central canal, which are thought to modulate MN excitability. These findings suggest that TRPM8-mediated cutaneous stimulation with low-load exercise promotes preferential recruitment of large MNs and is potentially useful as a new training method for rehabilitation.</description><subject>Acetyltransferase</subject><subject>Animals</subject><subject>c-fos</subject><subject>c-Fos protein</subject><subject>Choline</subject><subject>Choline O-acetyltransferase</subject><subject>Excitability</subject><subject>Exercise equipment</subject><subject>Exercise Test</subject><subject>Experiments</subject><subject>Fitness equipment</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Interneurons</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Motor neuron</subject><subject>Motor Neurons - physiology</subject><subject>Motor task performance</subject><subject>Motor units</subject><subject>Muscle function</subject><subject>Neuromodulation</subject><subject>Neurons</subject><subject>Original Paper</subject><subject>Physical Conditioning, Animal</subject><subject>Proto-Oncogene Proteins c-fos - genetics</subject><subject>Proto-Oncogene Proteins c-fos - metabolism</subject><subject>Pyrimidinones - pharmacology</subject><subject>Recruitment</subject><subject>Rehabilitation</subject><subject>Skin - drug effects</subject><subject>Somatosensory</subject><subject>Spinal cord</subject><subject>Transient receptor potential proteins</subject><subject>TRPM Cation Channels - genetics</subject><subject>TRPM Cation Channels - metabolism</subject><subject>TRPM8</subject><issn>1880-6546</issn><issn>1880-6562</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9Ul1rFTEQXUSxtfoHfJAFn7cm2Ww-QJBS_IKKIvU55Caz15Td5JpkL_TfO9etF4VS8pCZyTlnkslpmpeUnFNC5JtC2SBFR6juMCWy6x81p1Qp0olBsMfHmIuT5lkpN4RwoZl62pz0lCuGyWkzXn__9kV1M_hgK_jWLdVGSEtpSw3zMtkaUmzn5A8hFIxqym2EJWPZuhr2od62fskhbtuawfo5TBOSYbc7lAKSg4PnzZPRTgVe3O1nzY8P768vP3VXXz9-vry46pwkonaj005TDbKHkRIpe7Lh3jFBteYbqQc6At_4ASz3vQZPuORcMOslHTTxTvVnzbtVd7ds8E0OYs12MrscZptvTbLB_H8Sw0-zTXuD3ahkw4AKr-8Ucvq1QKnmJi054qUNw0kqSoTWD6J6RpRWSnBEdStqaycwIY4Je7otRMDWKcIYsHwhxdBjZ0IQf34PHpcHHOK9BLYSXE6lZBiPL6XEHDxiVo8Y9Ij54xHTI-nVvzM6Uv6aAgFvVwDgT-0DZFNcgOjQIxlcNT6Fh_R_AysTy5w</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Tamura, Kotaro</creator><creator>Sugita, Satoshi</creator><creator>Tokunaga, Tadayuki</creator><creator>Minegishi, Yoshihiko</creator><creator>Ota, Noriyasu</creator><general>Elsevier Inc</general><general>Springer</general><general>BioMed Central</general><general>Springer Japan</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20191101</creationdate><title>TRPM8-mediated cutaneous stimulation modulates motor neuron activity during treadmill stepping in mice</title><author>Tamura, Kotaro ; Sugita, Satoshi ; Tokunaga, Tadayuki ; Minegishi, Yoshihiko ; Ota, Noriyasu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c706t-fc9c919e73ef107730b4dc261994b7951fe4bd5ea4d39ed0474462ad71590dc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetyltransferase</topic><topic>Animals</topic><topic>c-fos</topic><topic>c-Fos protein</topic><topic>Choline</topic><topic>Choline O-acetyltransferase</topic><topic>Excitability</topic><topic>Exercise equipment</topic><topic>Exercise Test</topic><topic>Experiments</topic><topic>Fitness equipment</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Interneurons</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Motor neuron</topic><topic>Motor Neurons - physiology</topic><topic>Motor task performance</topic><topic>Motor units</topic><topic>Muscle function</topic><topic>Neuromodulation</topic><topic>Neurons</topic><topic>Original Paper</topic><topic>Physical Conditioning, Animal</topic><topic>Proto-Oncogene Proteins c-fos - genetics</topic><topic>Proto-Oncogene Proteins c-fos - metabolism</topic><topic>Pyrimidinones - pharmacology</topic><topic>Recruitment</topic><topic>Rehabilitation</topic><topic>Skin - drug effects</topic><topic>Somatosensory</topic><topic>Spinal cord</topic><topic>Transient receptor potential proteins</topic><topic>TRPM Cation Channels - genetics</topic><topic>TRPM Cation Channels - metabolism</topic><topic>TRPM8</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tamura, Kotaro</creatorcontrib><creatorcontrib>Sugita, Satoshi</creatorcontrib><creatorcontrib>Tokunaga, Tadayuki</creatorcontrib><creatorcontrib>Minegishi, Yoshihiko</creatorcontrib><creatorcontrib>Ota, Noriyasu</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of physiological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tamura, Kotaro</au><au>Sugita, Satoshi</au><au>Tokunaga, Tadayuki</au><au>Minegishi, Yoshihiko</au><au>Ota, Noriyasu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRPM8-mediated cutaneous stimulation modulates motor neuron activity during treadmill stepping in mice</atitle><jtitle>The journal of physiological sciences</jtitle><addtitle>J Physiol Sci</addtitle><date>2019-11-01</date><risdate>2019</risdate><volume>69</volume><issue>6</issue><spage>931</spage><epage>938</epage><pages>931-938</pages><issn>1880-6546</issn><eissn>1880-6562</eissn><abstract>Motor units are generally recruited from the smallest to the largest following the size principle, while cutaneous stimulation has the potential to affect spinal motor control. We aimed to examine the effects of stimulating transient receptor potential channel sub-family M8 (TRPM8) combined with exercise on the modulation of spinal motor neuron (MN) excitability. Mice were topically administrated 1.5% icilin on the hindlimbs, followed by treadmill stepping. Spinal cord sections were immunostained with antibodies against c-fos and choline acetyltransferase. Icilin stimulation did not change the number of c-fos+ MNs, but increased the average soma size of the c-fos+ MNs during low-speed treadmill stepping. Furthermore, icilin stimulation combined with stepping increased c-fos+ cholinergic interneurons near the central canal, which are thought to modulate MN excitability. These findings suggest that TRPM8-mediated cutaneous stimulation with low-load exercise promotes preferential recruitment of large MNs and is potentially useful as a new training method for rehabilitation.</abstract><cop>Japan</cop><pub>Elsevier Inc</pub><pmid>31482469</pmid><doi>10.1007/s12576-019-00707-3</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetyltransferase Animals c-fos c-Fos protein Choline Choline O-acetyltransferase Excitability Exercise equipment Exercise Test Experiments Fitness equipment Gene Expression Regulation - drug effects Interneurons Male Mice Mice, Inbred C57BL Motor neuron Motor Neurons - physiology Motor task performance Motor units Muscle function Neuromodulation Neurons Original Paper Physical Conditioning, Animal Proto-Oncogene Proteins c-fos - genetics Proto-Oncogene Proteins c-fos - metabolism Pyrimidinones - pharmacology Recruitment Rehabilitation Skin - drug effects Somatosensory Spinal cord Transient receptor potential proteins TRPM Cation Channels - genetics TRPM Cation Channels - metabolism TRPM8 |
| title | TRPM8-mediated cutaneous stimulation modulates motor neuron activity during treadmill stepping in mice |
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