Global Urinary Volatolomics with (GC×)GC-TOF-MS
Urinary volatolomics offers a noninvasive approach for disease detection and monitoring. Herein we present an improved methodology for global volatolomic profiling. Wide coverage was achieved by utilizing a multiphase sorbent for volatile organic compound (VOC) extraction. A single, midpolar column...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2023-11, Vol.95 (47), p.17170-17176 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Myridakis, Antonis Wen, Qing Boshier, Piers R Parker, Aaron G Belluomo, Ilaria Handakas, Evangelos Hanna, George B |
| description | Urinary volatolomics offers a noninvasive approach for disease detection and monitoring. Herein we present an improved methodology for global volatolomic profiling. Wide coverage was achieved by utilizing a multiphase sorbent for volatile organic compound (VOC) extraction. A single, midpolar column gas chromatography (GC) assay yielded substantially higher numbers of monitored VOCs compared to our previously reported single-sorbent method. Multidimensional GC (GC×GC) enhanced further biomarker discovery while data analysis was simplified by using a tile-based approach. At the same time, the required urine volume was reduced 5-fold from 2 to 0.4 mL. The applicability of the methodology was demonstrated in a pancreatic ductal adenocarcinoma cohort where previous findings were confirmed while a series of additional VOCs with diagnostic potential were discovered. |
| doi_str_mv | 10.1021/acs.analchem.3c02523 |
| format | Article |
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Herein we present an improved methodology for global volatolomic profiling. Wide coverage was achieved by utilizing a multiphase sorbent for volatile organic compound (VOC) extraction. A single, midpolar column gas chromatography (GC) assay yielded substantially higher numbers of monitored VOCs compared to our previously reported single-sorbent method. Multidimensional GC (GC×GC) enhanced further biomarker discovery while data analysis was simplified by using a tile-based approach. At the same time, the required urine volume was reduced 5-fold from 2 to 0.4 mL. 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Herein we present an improved methodology for global volatolomic profiling. Wide coverage was achieved by utilizing a multiphase sorbent for volatile organic compound (VOC) extraction. A single, midpolar column gas chromatography (GC) assay yielded substantially higher numbers of monitored VOCs compared to our previously reported single-sorbent method. Multidimensional GC (GC×GC) enhanced further biomarker discovery while data analysis was simplified by using a tile-based approach. At the same time, the required urine volume was reduced 5-fold from 2 to 0.4 mL. 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| subjects | Adenocarcinoma Biomarkers Chromatography, Gas Column chromatography Data analysis Disease detection Gas chromatography Gas Chromatography-Mass Spectrometry - methods Humans Organic compounds Sorbents Technical Note VOCs Volatile organic compounds Volatile Organic Compounds - analysis |
| title | Global Urinary Volatolomics with (GC×)GC-TOF-MS |
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