Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has been widely used for analyses of biomolecules and industrial materials. Surface-assisted laser desorption/ionization (SALDI) is studied to complement the ionization ability for the MALDI/MS. In this study, lab-made mist c...
Gespeichert in:
| Veröffentlicht in: | Mass Spectrometry 2023/11/25, Vol.12(1), pp.A0135-A0135 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | A0135 |
|---|---|
| container_issue | 1 |
| container_start_page | A0135 |
| container_title | Mass Spectrometry |
| container_volume | 12 |
| creator | Takata, Riko Nakabayashi, Yuji Hashimoto, Kotaro Miyazato, Akio Osaka, Issey |
| description | Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has been widely used for analyses of biomolecules and industrial materials. Surface-assisted laser desorption/ionization (SALDI) is studied to complement the ionization ability for the MALDI/MS. In this study, lab-made mist chemical vapor deposition (mist CVD) system was used to produce metal films as ionization assistance materials for SALDI/MS. The system could give Ag film from inexpensive silver trifluoroacetate solution rapidly and simply under atmospheric pressure. Phosphatidylcholines could be detected high sensitively and diacylglycerols (DAGs) could not be detected in MALDI/MS. In the SALDI/MS and the MS imaging with Ag film by mist CVD, both the phosphatidylcholines and the DAGs could be detected and the localized images. In the Ag film-SALDI/MS of lipids, not only Ag-adducted ions but also Na- and K-adducted ions were detected. The Ag film formed by the mist CVD to act as an ionization-assistance material and a cationization agent in SALDI would be useful in MS imaging of biological tissue sections. |
| doi_str_mv | 10.5702/massspectrometry.A0135 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10686700</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2914475400</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4175-fdfa86562d5a2512063fb3cc20e327496edfed292f4e42ddbc5ee489dd589e443</originalsourceid><addsrcrecordid>eNplkd1q3DAQhU1poSHJKxRBr51YsuWfq7Jsuu3CLi2kKb0TsjS2FWTL1Whb3Efr08XeDUt_bqSBOefTQSeK3tDkhhcJu-0lIo6ggnc9BD_drBKa8hfRBaNlHnNK85fHuYhpWnx7HV0jmjphOa8KyvlF9Hvby9YMLVkN0k5okLiGfO4cjp0MRk9Wdc6aAZDIQZM7I9VkWzsp8M4iecDFen_wjVQQr2Y2BtBkJxE8uQN0fgzGDbdbN5hfchnJfg5M7v9ITH6a0JE9BGnJxtiebJzvZ0g9kf2MI-sOeqPm5Vc5uoU6OjQL6ip61UiLcP18X0YPm_df1h_j3acP2_VqF6uMFjxudCPLnOdMc8k4ZUmeNnWqFEsgZUVW5aAb0KxiTQYZ07pWHCArK615WUGWpZfRuxN3PNRzMAVD8NKK0Zte-kk4acTfm8F0onU_BE3yMi-SZCa8fSZ49_0AGMSjO_j5w1GwimZZwbOjKj-plHeIHprzEzQRS9ni37LFsezZuDsZHzHIFs426YNRFv63USbochztZ5nqpBcwpE_Up8a6</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2914475400</pqid></control><display><type>article</type><title>Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition</title><source>J-STAGE Free</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Takata, Riko ; Nakabayashi, Yuji ; Hashimoto, Kotaro ; Miyazato, Akio ; Osaka, Issey</creator><creatorcontrib>Takata, Riko ; Nakabayashi, Yuji ; Hashimoto, Kotaro ; Miyazato, Akio ; Osaka, Issey</creatorcontrib><description>Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has been widely used for analyses of biomolecules and industrial materials. Surface-assisted laser desorption/ionization (SALDI) is studied to complement the ionization ability for the MALDI/MS. In this study, lab-made mist chemical vapor deposition (mist CVD) system was used to produce metal films as ionization assistance materials for SALDI/MS. The system could give Ag film from inexpensive silver trifluoroacetate solution rapidly and simply under atmospheric pressure. Phosphatidylcholines could be detected high sensitively and diacylglycerols (DAGs) could not be detected in MALDI/MS. In the SALDI/MS and the MS imaging with Ag film by mist CVD, both the phosphatidylcholines and the DAGs could be detected and the localized images. In the Ag film-SALDI/MS of lipids, not only Ag-adducted ions but also Na- and K-adducted ions were detected. The Ag film formed by the mist CVD to act as an ionization-assistance material and a cationization agent in SALDI would be useful in MS imaging of biological tissue sections.</description><identifier>ISSN: 2187-137X</identifier><identifier>EISSN: 2186-5116</identifier><identifier>DOI: 10.5702/massspectrometry.A0135</identifier><language>eng</language><publisher>Tokyo: The Mass Spectrometry Society of Japan</publisher><subject>Biomolecules ; Chemical vapor deposition ; Desorption ; diacylglycerols ; Imaging ; Ionization ; Lasers ; Lipids ; Mass spectrometry ; metal film ; Metal films ; Mist ; mist CVD ; Original ; phosphatidylcholines ; SALDI/MS imaging ; Scientific imaging ; Silver ; Tissues</subject><ispartof>Mass Spectrometry, 2023/11/25, Vol.12(1), pp.A0135-A0135</ispartof><rights>2023 Takata, Riko, Nakabayashi, Yuji, Hashimoto, Kotaro, Miyazato, Akio, and Osaka, Issey</rights><rights>2023. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2023 Riko Takata, Yuji Nakabayashi, Kotaro Hashimoto, Akio Miyazato, and Issey Osaka 2023 Riko Takata, Yuji Nakabayashi, Kotaro Hashimoto, Akio Miyazato, and Issey Osaka.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4175-fdfa86562d5a2512063fb3cc20e327496edfed292f4e42ddbc5ee489dd589e443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686700/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686700/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Takata, Riko</creatorcontrib><creatorcontrib>Nakabayashi, Yuji</creatorcontrib><creatorcontrib>Hashimoto, Kotaro</creatorcontrib><creatorcontrib>Miyazato, Akio</creatorcontrib><creatorcontrib>Osaka, Issey</creatorcontrib><title>Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition</title><title>Mass Spectrometry</title><addtitle>Mass Spectrometry</addtitle><description>Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has been widely used for analyses of biomolecules and industrial materials. Surface-assisted laser desorption/ionization (SALDI) is studied to complement the ionization ability for the MALDI/MS. In this study, lab-made mist chemical vapor deposition (mist CVD) system was used to produce metal films as ionization assistance materials for SALDI/MS. The system could give Ag film from inexpensive silver trifluoroacetate solution rapidly and simply under atmospheric pressure. Phosphatidylcholines could be detected high sensitively and diacylglycerols (DAGs) could not be detected in MALDI/MS. In the SALDI/MS and the MS imaging with Ag film by mist CVD, both the phosphatidylcholines and the DAGs could be detected and the localized images. In the Ag film-SALDI/MS of lipids, not only Ag-adducted ions but also Na- and K-adducted ions were detected. The Ag film formed by the mist CVD to act as an ionization-assistance material and a cationization agent in SALDI would be useful in MS imaging of biological tissue sections.</description><subject>Biomolecules</subject><subject>Chemical vapor deposition</subject><subject>Desorption</subject><subject>diacylglycerols</subject><subject>Imaging</subject><subject>Ionization</subject><subject>Lasers</subject><subject>Lipids</subject><subject>Mass spectrometry</subject><subject>metal film</subject><subject>Metal films</subject><subject>Mist</subject><subject>mist CVD</subject><subject>Original</subject><subject>phosphatidylcholines</subject><subject>SALDI/MS imaging</subject><subject>Scientific imaging</subject><subject>Silver</subject><subject>Tissues</subject><issn>2187-137X</issn><issn>2186-5116</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNplkd1q3DAQhU1poSHJKxRBr51YsuWfq7Jsuu3CLi2kKb0TsjS2FWTL1Whb3Efr08XeDUt_bqSBOefTQSeK3tDkhhcJu-0lIo6ggnc9BD_drBKa8hfRBaNlHnNK85fHuYhpWnx7HV0jmjphOa8KyvlF9Hvby9YMLVkN0k5okLiGfO4cjp0MRk9Wdc6aAZDIQZM7I9VkWzsp8M4iecDFen_wjVQQr2Y2BtBkJxE8uQN0fgzGDbdbN5hfchnJfg5M7v9ITH6a0JE9BGnJxtiebJzvZ0g9kf2MI-sOeqPm5Vc5uoU6OjQL6ip61UiLcP18X0YPm_df1h_j3acP2_VqF6uMFjxudCPLnOdMc8k4ZUmeNnWqFEsgZUVW5aAb0KxiTQYZ07pWHCArK615WUGWpZfRuxN3PNRzMAVD8NKK0Zte-kk4acTfm8F0onU_BE3yMi-SZCa8fSZ49_0AGMSjO_j5w1GwimZZwbOjKj-plHeIHprzEzQRS9ni37LFsezZuDsZHzHIFs426YNRFv63USbochztZ5nqpBcwpE_Up8a6</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>Takata, Riko</creator><creator>Nakabayashi, Yuji</creator><creator>Hashimoto, Kotaro</creator><creator>Miyazato, Akio</creator><creator>Osaka, Issey</creator><general>The Mass Spectrometry Society of Japan</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>2023</creationdate><title>Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition</title><author>Takata, Riko ; Nakabayashi, Yuji ; Hashimoto, Kotaro ; Miyazato, Akio ; Osaka, Issey</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4175-fdfa86562d5a2512063fb3cc20e327496edfed292f4e42ddbc5ee489dd589e443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biomolecules</topic><topic>Chemical vapor deposition</topic><topic>Desorption</topic><topic>diacylglycerols</topic><topic>Imaging</topic><topic>Ionization</topic><topic>Lasers</topic><topic>Lipids</topic><topic>Mass spectrometry</topic><topic>metal film</topic><topic>Metal films</topic><topic>Mist</topic><topic>mist CVD</topic><topic>Original</topic><topic>phosphatidylcholines</topic><topic>SALDI/MS imaging</topic><topic>Scientific imaging</topic><topic>Silver</topic><topic>Tissues</topic><toplevel>online_resources</toplevel><creatorcontrib>Takata, Riko</creatorcontrib><creatorcontrib>Nakabayashi, Yuji</creatorcontrib><creatorcontrib>Hashimoto, Kotaro</creatorcontrib><creatorcontrib>Miyazato, Akio</creatorcontrib><creatorcontrib>Osaka, Issey</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takata, Riko</au><au>Nakabayashi, Yuji</au><au>Hashimoto, Kotaro</au><au>Miyazato, Akio</au><au>Osaka, Issey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition</atitle><jtitle>Mass Spectrometry</jtitle><addtitle>Mass Spectrometry</addtitle><date>2023</date><risdate>2023</risdate><volume>12</volume><issue>1</issue><spage>A0135</spage><epage>A0135</epage><pages>A0135-A0135</pages><artnum>A0135</artnum><issn>2187-137X</issn><eissn>2186-5116</eissn><abstract>Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has been widely used for analyses of biomolecules and industrial materials. Surface-assisted laser desorption/ionization (SALDI) is studied to complement the ionization ability for the MALDI/MS. In this study, lab-made mist chemical vapor deposition (mist CVD) system was used to produce metal films as ionization assistance materials for SALDI/MS. The system could give Ag film from inexpensive silver trifluoroacetate solution rapidly and simply under atmospheric pressure. Phosphatidylcholines could be detected high sensitively and diacylglycerols (DAGs) could not be detected in MALDI/MS. In the SALDI/MS and the MS imaging with Ag film by mist CVD, both the phosphatidylcholines and the DAGs could be detected and the localized images. In the Ag film-SALDI/MS of lipids, not only Ag-adducted ions but also Na- and K-adducted ions were detected. The Ag film formed by the mist CVD to act as an ionization-assistance material and a cationization agent in SALDI would be useful in MS imaging of biological tissue sections.</abstract><cop>Tokyo</cop><pub>The Mass Spectrometry Society of Japan</pub><doi>10.5702/massspectrometry.A0135</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2187-137X |
| ispartof | Mass Spectrometry, 2023/11/25, Vol.12(1), pp.A0135-A0135 |
| issn | 2187-137X 2186-5116 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10686700 |
| source | J-STAGE Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Biomolecules Chemical vapor deposition Desorption diacylglycerols Imaging Ionization Lasers Lipids Mass spectrometry metal film Metal films Mist mist CVD Original phosphatidylcholines SALDI/MS imaging Scientific imaging Silver Tissues |
| title | Imaging Analysis of Phosphatidylcholines and Diacylglycerols Using Surface-Assisted Laser Desorption/Ionization Mass Spectrometry with Metal Film Formed by Mist Chemical Vapor Deposition |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T11%3A09%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Imaging%20Analysis%20of%20Phosphatidylcholines%20and%20Diacylglycerols%20Using%20Surface-Assisted%20Laser%20Desorption/Ionization%20Mass%20Spectrometry%20with%20Metal%20Film%20Formed%20by%20Mist%20Chemical%20Vapor%20Deposition&rft.jtitle=Mass%20Spectrometry&rft.au=Takata,%20Riko&rft.date=2023&rft.volume=12&rft.issue=1&rft.spage=A0135&rft.epage=A0135&rft.pages=A0135-A0135&rft.artnum=A0135&rft.issn=2187-137X&rft.eissn=2186-5116&rft_id=info:doi/10.5702/massspectrometry.A0135&rft_dat=%3Cproquest_pubme%3E2914475400%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2914475400&rft_id=info:pmid/&rfr_iscdi=true |