Iron Oxide Nanoparticles Conjugated to Thiosemicarbazone Reduce the Survival of Cancer Cells by Increasing the Gene Expression of MicroRNA let-7c in Lung Cancer A549 Cells
Cancer cells have a higher demand for iron to grow and proliferate. A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray...
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| Veröffentlicht in: | Archives of Iranian medicine 2022-12, Vol.25 (12), p.807-816 |
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| creator | Habibi, Alireza Bakhshi, Nesa Moradi Shoili, Zeinab Amirmozafari, Nour |
| description | Cancer cells have a higher demand for iron to grow and proliferate. A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and zeta potential.
MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe
O
@Glu/BTSC on changes in gene expression of
(
), respectively. The specifications of Fe
O
@ Glu/BTSC were confirmed at 5 nm.
Fe3O4@Glu/BTSC was more effective than BTSC and Fe
O
on A549 cells (IC
=166.77 µg/mL) but its effect on healthy cells was smaller (CC
=189.15 µg/mL). The drug selectivity index (SI) was calculated to be 1.13. The initial apoptosis rate was 46.33% for Fe
O
@Glu/BTSC, 28.27% for BTSC and 26.02% for Fe
O
. BTSC and BTSC@Fe
O
inhibited the cell cycle progression in the Sub-G1 and S phases.
expression was 6.9 times higher in treated cells compared to the control group. The expression rate was 2.2 with BTSC compared to the control group and 1.6 times for Fe
O
.
Fe
O
@Glu/BTSC has proper anti-proliferative effects against lung cancer cells by increasing the expression of
and inhibiting the cell cycle with the apoptosis activation pathway. |
| doi_str_mv | 10.34172/aim.2022.126 |
| format | Article |
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MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe
O
@Glu/BTSC on changes in gene expression of
(
), respectively. The specifications of Fe
O
@ Glu/BTSC were confirmed at 5 nm.
Fe3O4@Glu/BTSC was more effective than BTSC and Fe
O
on A549 cells (IC
=166.77 µg/mL) but its effect on healthy cells was smaller (CC
=189.15 µg/mL). The drug selectivity index (SI) was calculated to be 1.13. The initial apoptosis rate was 46.33% for Fe
O
@Glu/BTSC, 28.27% for BTSC and 26.02% for Fe
O
. BTSC and BTSC@Fe
O
inhibited the cell cycle progression in the Sub-G1 and S phases.
expression was 6.9 times higher in treated cells compared to the control group. The expression rate was 2.2 with BTSC compared to the control group and 1.6 times for Fe
O
.
Fe
O
@Glu/BTSC has proper anti-proliferative effects against lung cancer cells by increasing the expression of
and inhibiting the cell cycle with the apoptosis activation pathway.</description><identifier>ISSN: 1029-2977</identifier><identifier>EISSN: 1735-3947</identifier><identifier>DOI: 10.34172/aim.2022.126</identifier><identifier>PMID: 37543908</identifier><language>eng</language><publisher>Iran: Academy of Medical Sciences of I.R. Iran</publisher><subject>A549 Cells ; Apoptosis ; Cell cycle ; Gene Expression ; Humans ; Iron ; Lung cancer ; Lung Neoplasms - genetics ; Magnetic Iron Oxide Nanoparticles ; MicroRNAs ; MicroRNAs - genetics ; Nanoparticles ; Original ; Thiosemicarbazones - pharmacology</subject><ispartof>Archives of Iranian medicine, 2022-12, Vol.25 (12), p.807-816</ispartof><rights>2022 The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>2022. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 The Author(s). 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-daf7e96be07f6c2266aea2a6e6cdbff959a9ddbe9e031e0b60591df58401ca213</citedby><orcidid>0000-0001-5186-8406 ; 0000-0002-3672-5869</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685841/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685841/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37543908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Habibi, Alireza</creatorcontrib><creatorcontrib>Bakhshi, Nesa</creatorcontrib><creatorcontrib>Moradi Shoili, Zeinab</creatorcontrib><creatorcontrib>Amirmozafari, Nour</creatorcontrib><title>Iron Oxide Nanoparticles Conjugated to Thiosemicarbazone Reduce the Survival of Cancer Cells by Increasing the Gene Expression of MicroRNA let-7c in Lung Cancer A549 Cells</title><title>Archives of Iranian medicine</title><addtitle>Arch Iran Med</addtitle><description>Cancer cells have a higher demand for iron to grow and proliferate. A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and zeta potential.
MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe
O
@Glu/BTSC on changes in gene expression of
(
), respectively. The specifications of Fe
O
@ Glu/BTSC were confirmed at 5 nm.
Fe3O4@Glu/BTSC was more effective than BTSC and Fe
O
on A549 cells (IC
=166.77 µg/mL) but its effect on healthy cells was smaller (CC
=189.15 µg/mL). The drug selectivity index (SI) was calculated to be 1.13. The initial apoptosis rate was 46.33% for Fe
O
@Glu/BTSC, 28.27% for BTSC and 26.02% for Fe
O
. BTSC and BTSC@Fe
O
inhibited the cell cycle progression in the Sub-G1 and S phases.
expression was 6.9 times higher in treated cells compared to the control group. The expression rate was 2.2 with BTSC compared to the control group and 1.6 times for Fe
O
.
Fe
O
@Glu/BTSC has proper anti-proliferative effects against lung cancer cells by increasing the expression of
and inhibiting the cell cycle with the apoptosis activation pathway.</description><subject>A549 Cells</subject><subject>Apoptosis</subject><subject>Cell cycle</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Iron</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - genetics</subject><subject>Magnetic Iron Oxide Nanoparticles</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>Nanoparticles</subject><subject>Original</subject><subject>Thiosemicarbazones - pharmacology</subject><issn>1029-2977</issn><issn>1735-3947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpVkV1v0zAUhiMEYmNwyS2yxHWKPxK7vkJVNEalskljXFsnzknrKrWLnVQbf4k_ibeWCa5syc953mO9RfGe0ZmomOKfwO1mnHI-Y1y-KM6ZEnUpdKVe5jvluuRaqbPiTUpbSitRM_G6OBOqroSm8_Pi9zIGT27uXYfkGnzYQxydHTCRJvjttIYROzIGcrdxIeHOWYgt_AoeyS12k0UybpB8n-LBHWAgoScNeIuRNDgMibQPZOltREjOr5_QK8yjl_f7iCm5nJwnvjkbw-31ggw4lsoS58lqyvjJtKgrfdS9LV71MCR8dzovih9fLu-ar-Xq5mrZLFalrZgcyw56hVq2SFUvLedSAgIHidJ2bd_rWoPuuhY1UsGQtpLWmnV9Pa8os8CZuCg-H737qd1hZ9GPEQazj24H8cEEcOb_F-82Zh0OhlE5z5pHw8eTIYafE6bRbMMUfV7a8Bwja85UlanySOX_pxSxf45g1DyVa3K55rFck8vN_Id_93qm_7Yp_gCjm6MM</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Habibi, Alireza</creator><creator>Bakhshi, Nesa</creator><creator>Moradi Shoili, Zeinab</creator><creator>Amirmozafari, Nour</creator><general>Academy of Medical Sciences of I.R. 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A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and zeta potential.
MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe
O
@Glu/BTSC on changes in gene expression of
(
), respectively. The specifications of Fe
O
@ Glu/BTSC were confirmed at 5 nm.
Fe3O4@Glu/BTSC was more effective than BTSC and Fe
O
on A549 cells (IC
=166.77 µg/mL) but its effect on healthy cells was smaller (CC
=189.15 µg/mL). The drug selectivity index (SI) was calculated to be 1.13. The initial apoptosis rate was 46.33% for Fe
O
@Glu/BTSC, 28.27% for BTSC and 26.02% for Fe
O
. BTSC and BTSC@Fe
O
inhibited the cell cycle progression in the Sub-G1 and S phases.
expression was 6.9 times higher in treated cells compared to the control group. The expression rate was 2.2 with BTSC compared to the control group and 1.6 times for Fe
O
.
Fe
O
@Glu/BTSC has proper anti-proliferative effects against lung cancer cells by increasing the expression of
and inhibiting the cell cycle with the apoptosis activation pathway.</abstract><cop>Iran</cop><pub>Academy of Medical Sciences of I.R. Iran</pub><pmid>37543908</pmid><doi>10.34172/aim.2022.126</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5186-8406</orcidid><orcidid>https://orcid.org/0000-0002-3672-5869</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Archives of Iranian medicine, 2022-12, Vol.25 (12), p.807-816 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | A549 Cells Apoptosis Cell cycle Gene Expression Humans Iron Lung cancer Lung Neoplasms - genetics Magnetic Iron Oxide Nanoparticles MicroRNAs MicroRNAs - genetics Nanoparticles Original Thiosemicarbazones - pharmacology |
| title | Iron Oxide Nanoparticles Conjugated to Thiosemicarbazone Reduce the Survival of Cancer Cells by Increasing the Gene Expression of MicroRNA let-7c in Lung Cancer A549 Cells |
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